Dorey, Narimane; Lançon, Pauline; Thorndyke, Mike; Dupont, Sam (2013): Data S1. Results of the analyses considering developmental time, supplementary figures and tables & Video S1. PANGAEA, https://doi.org/10.1594/PANGAEA.819167, Supplement to: Dorey, N et al. (2013): Assessing physiological tipping point of sea urchin larvae exposed to a broad range of pH. Global Change Biology, 19(11), 3355-3367, https://doi.org/10.1111/gcb.12276
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Our ability to project the impact of global change on marine ecosystem is limited by our poor understanding on how to predict species sensitivity. For example, the impact of ocean acidification is highly species-specific, even in closely related taxa. The aim of this study was to test the hypothesis that the tolerance range of a given species to decreased pH corresponds to their natural range of exposure. Larvae of the green sea urchin Strongylocentrotus droebachiensis were cultured from fertilization to metamorphic competence (29 days) under a wide range of pH (from pHT = 8.0/pCO2 ~ 480 ?atm to pHT = 6.5/pCO2 ~ 20 000 ?atm) covering present (from pHT 8.7 to 7.6), projected near-future variability (from pHT 8.3 to 7.2) and beyond. Decreasing pH impacted all tested parameters (mortality, symmetry, growth, morphometry and respiration). Development of normal, although showing morphological plasticity, swimming larvae was possible as low as pHT >= 7.0. Within that range, decreasing pH increased mortality and asymmetry and decreased body length (BL) growth rate. Larvae raised at lowered pH and with similar BL had shorter arms and a wider body. Relative to a given BL, respiration rates and stomach volume both increased with decreasing pH suggesting changes in energy budget. At the lowest pHs (pHT <= 6.5), all the tested parameters were strongly negatively affected and no larva survived past 13 days post fertilization. In conclusion, sea urchin larvae appeared to be highly plastic when exposed to decreased pH until a physiological tipping point at pHT = 7.0. However, this plasticity was associated with direct (increased mortality) and indirect (decreased growth) consequences for fitness.
Video S1. Animated image of the morphology of an average Strongylocentrotus droebachiensis 350 µm larvae, as extrapolated using the results of this experiment (upper right corner: pH treatment and days of development needed to reach a 350 µm BL). File to open with a web browser or any other software supporting animated images.