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Felden, Janine; Boetius, Antje (2013): Pore water geochemistry of Håkon Mosby mud volcano sediments measured at station PS74/169-1_PUC-15 (Sample 1, new flow surface). Max-Planck-Institut für Marine Mikrobiologie, PANGAEA,, In supplement to: Ruff, S Emil; Felden, Janine; Gruber-Vodicka, Harald R; Marcon, Yann; Knittel, Katrin; Ramette, Alban; Boetius, Antje (2018): In situ development of a methanotrophic microbiome in deep-sea sediments. The ISME Journal,

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Pore water and turnover rates were determined for surface sediment cores obtained in 2009 and 2010. The pore water was extracted with Rhizons (Rhizon CSS: length 5 cm, pore diameter 0.15 µm; Rhizosphere Research Products, Wageningen, Netherlands) in 1 cm-resolution and immediately fixed in 5% zinc acetate (ZnAc) solution for sulfate, and sulfide analyses. The samples were diluted, filtered and the concentrations measured with non-suppressed anion exchange chromatography (Waters IC-Pak anion exchange column, waters 430 conductivity detector). The total sulfide concentrations (H2S + HS- + S**2-) were determined using the diamine complexation method (doi:10.4319/lo.1969.14.3.0454). Samples for dissolved inorganic carbon (DIC) and alkalinity measurements were preserved by adding 2 µl saturated mercury chloride (HgCl2) solution and stored headspace-free in gas-tight glass vials. DIC and alkalinity were measured using the flow injection method (detector VWR scientific model 1054) (doi:10.4319/lo.1992.37.5.1113). Dissolved sulfide was eliminated prior to the DIC measurement by adding 0.5 M molybdate solution (doi:10.4319/lo.1995.40.5.1011). Nutrient subsamples (10 - 15 ml) were stored at – 20 °C prior to concentration measurements with a Skalar Continuous-Flow Analyzer (doi:10.1002/9783527613984).
Latitude: 72.005000 * Longitude: 14.726402
Date/Time Start: 2009-07-23T18:22:43 * Date/Time End: 2009-07-23T18:22:43
Minimum DEPTH, sediment/rock: 0.005 m * Maximum DEPTH, sediment/rock: 0.110 m
PS74/169-1_PUC-15 * Latitude: 72.005000 * Longitude: 14.726402 * Date/Time: 2009-07-23T18:22:43 * Elevation: -1261.0 m * Location: Håkon Mosby Mud Volcano * Campaign: ARK-XXIV/2 (PS74) * Basis: Polarstern * Method/Device: Push corer (PUC)
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1DEPTH, sediment/rockDepthmGeocode
2SulfideS2-µmol/lFelden, JaninePhotometer, methylene blue (Cline 1969)
3Sulfate[SO4]2-mmol/lFelden, JanineIon chromatography
4Carbon, inorganic, dissolvedDICmmol/lFelden, JanineFlow injection analysis (Hall and Aller 1992)CO2 + HCO3- + CO32-
5Alkalinity, totalATmmol(eq)/lFelden, JanineTwo-point titration (Edmond 1970)
6SilicateSi(OH)4µmol/lFelden, JanineSeawater analysis after Grasshoff et al., 1983 (Verlag Chemie GmbH Weinheim)
7Phosphate[PO4]3-µmol/lFelden, JanineSeawater analysis after Grasshoff et al., 1983 (Verlag Chemie GmbH Weinheim)
8Ammonium[NH4]+µmol/lFelden, JanineSeawater analysis after Grasshoff et al., 1983 (Verlag Chemie GmbH Weinheim)
74 data points

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