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Hoogstraten, Astrid; Timmermans, Klaas R; de Baar, Hein J W (2012): Seawater carbonate chemistry, nutrients, chlorophyll and diatom Proboscia alata during experiments, 2012. PANGAEA,, Supplement to: Hoogstraten, A et al. (2012): Morphological and physiological effects in Proboscia alata (bacillariophyceae) grown under different light and Co2 conditions of the modern Southern Ocean. Journal of Phycology, 48(3), 559-568,

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The combined effects of different light and aqueous CO2 conditions were assessed for the Southern Ocean diatom Proboscia alata(Brightwell) Sundström in laboratory experiments. Selected culture conditions (light and CO2(aq)) were representative for the natural ranges in the modern Southern Ocean. Light conditions were 40 (low) and 240 (high) µmol photons/m**2/s. The three CO2(aq) conditions ranged from 8 to 34 µmol/kg CO2(aq) (equivalent to a pCO2 from 137 to 598 µatm, respectively). Clear morphological changes were induced by these different CO2(aq) conditions. Cells in low [CO2(aq)] formed spirals, while many cells in high [CO2(aq)] disintegrated. Cell size and volume were significantly affected by the different CO2(aq) concentrations. Increasing CO2(aq) concentrations led to an increase in particulate organic carbon concentrations per cell in the high light cultures, with exactly the opposite happening in the low light cultures. However, other parameters measured were not influenced by the range of CO2(aq) treatments. This included growth rates, chlorophyll aconcentration and photosynthetic yield (FV/FM). Different light treatments had a large effect on nutrient uptake. High light conditions caused an increased nutrient uptake rate compared to cells grown in low light conditions. Light and CO2 conditions co-determined in various ways the response of P. alata to changing environmental conditions. Overall P. alata appeared to be well adapted to the natural variability in light availability and CO2(aq) concentration of the modern Southern Ocean. Nevertheless, our results showed that P. alata is susceptible to future changes in inorganic carbon concentrations in the Southern Ocean.
Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Light; North Atlantic; Ochrophyta; Pelagos; Phytoplankton; Primary production/Photosynthesis; Proboscia alata; Single species
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). Data extracted in the frame of a joint ICSTI/PANGAEA IPY effort, see
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Experimental treatmentExp treatHoogstraten, Astrid
2SalinitySalHoogstraten, Astrid
3Temperature, waterTemp°CHoogstraten, Astrid
4Temperature, standard deviationT std dev±Hoogstraten, Astrid
5Light:Dark cycleL:Dhh:hhHoogstraten, Astrid
6Radiation, photosynthetically activePARµmol/m2/sHoogstraten, Astrid
7Alkalinity, totalATµmol/kgHoogstraten, AstridPotentiometric titration, VINDTA (marianda)
8Alkalinity, total, standard deviationAT std dev±Hoogstraten, Astrid
9Carbon, inorganic, dissolvedDICµmol/kgHoogstraten, Astrid
10Carbon, inorganic, dissolved, standard deviationDIC std dev±Hoogstraten, Astrid
11Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmHoogstraten, AstridCalculated using CO2SYS
12Carbon dioxide, partial pressure, standard deviationpCO2 std dev±Hoogstraten, Astrid
13Carbon dioxideCO2µmol/kgHoogstraten, AstridCalculated using CO2SYS
14Carbon dioxide, standard deviationCO2 std dev±Hoogstraten, Astrid
15Bicarbonate ion[HCO3]-µmol/kgHoogstraten, AstridCalculated using CO2SYS
16Bicarbonate ion, standard deviation[HCO3]- std dev±Hoogstraten, Astrid
17Carbonate ion[CO3]2-µmol/kgHoogstraten, AstridCalculated using CO2SYS
18Carbonate ion, standard deviation[CO3]2- std dev±Hoogstraten, Astrid
19pHpHHoogstraten, AstridCalculated using CO2SYS
20pH, standard deviationpH std dev±Hoogstraten, Astrid
21ReplicatesRepl#Hoogstraten, Astrid
22Proboscia alata, lengthP. alata lµmHoogstraten, AstridMicroscopy
23Proboscia alata, length, standard deviationP. alata l std dev±Hoogstraten, Astrid
24Proboscia alata, diameterP. alata diamµmHoogstraten, AstridMicroscopy
25Proboscia alata, diameter, standard deviationP. alata diam std dev±Hoogstraten, Astrid
26Proboscia alata, volumeP. alata volµm3Hoogstraten, AstridMicroscopy
27Proboscia alata, volume, standard deviationP. alata vol std dev±Hoogstraten, Astrid
28Photosynthetic efficiencyFV/FMHoogstraten, Astridsee reference(s)
29Photosynthetic efficiency, standard deviationalpha std dev±Hoogstraten, Astrid
30Chlorophyll a per cellChl apg/#Hoogstraten, AstridSpectrofluorometry
31Chlorophyll a, standard deviationChl a std dev±Hoogstraten, Astrid
32Nitrate uptake rate per cellNO3 upt ratefmol/#/dayHoogstraten, AstridAuto-analyzer, Technicon Traacs 800
33Nitrate uptake rate, standard deviationNO3 upt rate std dev±Hoogstraten, Astrid
34Phosphate uptake rate per cellPO4 upt ratefmol/#/dayHoogstraten, AstridAuto-analyzer, Technicon Traacs 800
35Phosphate uptake rate, standard deviationPO4 upt rate std dev±Hoogstraten, Astrid
36Silicon uptake rate per cellSi upt ratepmol/#/dayHoogstraten, AstridAuto-analyzer, Technicon Traacs 800
37Silicon uptake, standard deviationSi upt std dev±Hoogstraten, Astrid
38Nitrogen/Phosphorus ratioN/PHoogstraten, Astrid
39Nitrogen/Phosphorus ratio, standard deviationN/P std dev±Hoogstraten, Astrid
40Nitrogen/Silicon ratioN/SiHoogstraten, Astrid
41Standard deviationStd dev±Hoogstraten, AstridN/Si ratio
42Silicon/Phosphorus ratioSi/PHoogstraten, Astrid
43Silicon/Phosphorus ratio, standard deviationSi/P std dev±Hoogstraten, Astrid
44Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
45pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale
46Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
47Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
48Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
49Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
50Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
51Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
52Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
304 data points

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