Citation:

j.1529-8817.2012.01148.x

Name: Seawater carbonate chemistry, nutrients, chlorophyll and diatom Proboscia alata during experiments, 2012
Published: 2012-03-29
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Light; North Atlantic; Ochrophyta; Pelagos; Phytoplankton; Primary production/Photosynthesis; Proboscia alata; Single species

Always quote citation above when using data! You can download the citation in several formats below.

RIS Citation BibTeX Citation Text Citation Facebook Twitter

Abstract:

The combined effects of different light and aqueous CO2 conditions were assessed for the Southern Ocean diatom Proboscia alata(Brightwell) Sundström in laboratory experiments. Selected culture conditions (light and CO2(aq)) were representative for the natural ranges in the modern Southern Ocean. Light conditions were 40 (low) and 240 (high) µmol photons/m**2/s. The three CO2(aq) conditions ranged from 8 to 34 µmol/kg CO2(aq) (equivalent to a pCO2 from 137 to 598 µatm, respectively). Clear morphological changes were induced by these different CO2(aq) conditions. Cells in low [CO2(aq)] formed spirals, while many cells in high [CO2(aq)] disintegrated. Cell size and volume were significantly affected by the different CO2(aq) concentrations. Increasing CO2(aq) concentrations led to an increase in particulate organic carbon concentrations per cell in the high light cultures, with exactly the opposite happening in the low light cultures. However, other parameters measured were not influenced by the range of CO2(aq) treatments. This included growth rates, chlorophyll aconcentration and photosynthetic yield (FV/FM). Different light treatments had a large effect on nutrient uptake. High light conditions caused an increased nutrient uptake rate compared to cells grown in low light conditions. Light and CO2 conditions co-determined in various ways the response of P. alata to changing environmental conditions. Overall P. alata appeared to be well adapted to the natural variability in light availability and CO2(aq) concentration of the modern Southern Ocean. Nevertheless, our results showed that P. alata is susceptible to future changes in inorganic carbon concentrations in the Southern Ocean.

Keyword(s):

Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Light; North Atlantic; Ochrophyta; Pelagos; Phytoplankton; Primary production/Photosynthesis; Proboscia alata; Single species

Project(s):

European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)

European Project on Ocean Acidification (EPOCA)

International Polar Year (2007-2008) (IPY)

Ocean Acidification International Coordination Centre (OA-ICC)

Funding:

Seventh Framework Programme (FP7), grant/award no. 211384 : European Project on Ocean Acidification

Sixth Framework Programme (FP6), grant/award no. 511106 : European network of excellence for Ocean Ecosystems Analysis

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). Data extracted in the frame of a joint ICSTI/PANGAEA IPY effort, see http://doi.pangaea.de/10.1594/PANGAEA.150150

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Experimental treatment	Exp treat		Hoogstraten, Astrid
2	Salinity	Sal		Hoogstraten, Astrid
3	Temperature, water	Temp	°C	Hoogstraten, Astrid
4	Temperature, standard deviation	T std dev	±	Hoogstraten, Astrid
5	Light:Dark cycle	L:D	hh:hh	Hoogstraten, Astrid
6	Radiation, photosynthetically active	PAR	µmol/m²/s	Hoogstraten, Astrid
7	Alkalinity, total	AT	µmol/kg	Hoogstraten, Astrid	Potentiometric titration, VINDTA (marianda)
8	Alkalinity, total, standard deviation	AT std dev	±	Hoogstraten, Astrid
9	Carbon, inorganic, dissolved	DIC	µmol/kg	Hoogstraten, Astrid
10	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Hoogstraten, Astrid
11	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Hoogstraten, Astrid	Calculated using CO2SYS
12	Carbon dioxide, partial pressure, standard deviation	pCO2 std dev	±	Hoogstraten, Astrid
13	Carbon dioxide	CO2	µmol/kg	Hoogstraten, Astrid	Calculated using CO2SYS
14	Carbon dioxide, standard deviation	CO2 std dev	±	Hoogstraten, Astrid
15	Bicarbonate ion	[HCO3]-	µmol/kg	Hoogstraten, Astrid	Calculated using CO2SYS
16	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Hoogstraten, Astrid
17	Carbonate ion	[CO3]2-	µmol/kg	Hoogstraten, Astrid	Calculated using CO2SYS
18	Carbonate ion, standard deviation	[CO3]2- std dev	±	Hoogstraten, Astrid
19	pH	pH		Hoogstraten, Astrid	Calculated using CO2SYS
20	pH, standard deviation	pH std dev	±	Hoogstraten, Astrid
21	Replicates	Repl	#	Hoogstraten, Astrid
22	Proboscia alata, length	P. alata l	µm	Hoogstraten, Astrid	Microscopy
23	Proboscia alata, length, standard deviation	P. alata l std dev	±	Hoogstraten, Astrid
24	Proboscia alata, diameter	P. alata diam	µm	Hoogstraten, Astrid	Microscopy
25	Proboscia alata, diameter, standard deviation	P. alata diam std dev	±	Hoogstraten, Astrid
26	Proboscia alata, volume	P. alata vol	µm³	Hoogstraten, Astrid	Microscopy
27	Proboscia alata, volume, standard deviation	P. alata vol std dev	±	Hoogstraten, Astrid
28	Photosynthetic efficiency	FV/FM		Hoogstraten, Astrid	see reference(s)
29	Photosynthetic efficiency, standard deviation	alpha std dev	±	Hoogstraten, Astrid
30	Chlorophyll a per cell	Chl a/cell	pg/#	Hoogstraten, Astrid	Spectrofluorometry
31	Chlorophyll a, standard deviation	Chl a std dev	±	Hoogstraten, Astrid
32	Nitrate uptake rate per cell	NO3 upt rate/cell	fmol/#/day	Hoogstraten, Astrid	Auto-analyzer, Technicon Traacs 800
33	Nitrate uptake rate, standard deviation	NO3 upt rate std dev	±	Hoogstraten, Astrid
34	Phosphate uptake rate per cell	PO4 upt rate/cell	fmol/#/day	Hoogstraten, Astrid	Auto-analyzer, Technicon Traacs 800
35	Phosphate uptake rate, standard deviation	PO4 upt rate std dev	±	Hoogstraten, Astrid
36	Silicon uptake rate per cell	Si upt rate/cell	pmol/#/day	Hoogstraten, Astrid	Auto-analyzer, Technicon Traacs 800
37	Silicon uptake, standard deviation	Si upt std dev	±	Hoogstraten, Astrid
38	Nitrogen/Phosphorus ratio	N/P		Hoogstraten, Astrid
39	Nitrogen/Phosphorus ratio, standard deviation	N/P std dev	±	Hoogstraten, Astrid
40	Nitrogen/Silicon ratio	N/Si		Hoogstraten, Astrid
41	Standard deviation	Std dev	±	Hoogstraten, Astrid		N/Si ratio
42	Silicon/Phosphorus ratio	Si/P		Hoogstraten, Astrid
43	Silicon/Phosphorus ratio, standard deviation	Si/P std dev	±	Hoogstraten, Astrid
44	Carbonate system computation flag	CSC flag		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
45	pH	pH		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)	Total scale
46	Carbon dioxide	CO2	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
47	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
48	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
49	Bicarbonate ion	[HCO3]-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
50	Carbonate ion	[CO3]2-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
51	Aragonite saturation state	Omega Arg		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
52	Calcite saturation state	Omega Cal		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

304 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Exp treat	2 Sal	3 Temp [°C]	4 T std dev [±]	5 L:D [hh:hh]	6 PAR [µmol/m²/s]	7 AT [µmol/kg] (Potentiometric titration, VIN...)	8 AT std dev [±]	9 DIC [µmol/kg]	10 DIC std dev [±]	11 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	12 pCO2 std dev [±]	13 CO2 [µmol/kg] (Calculated using CO2SYS)	14 CO2 std dev [±]	15 [HCO3]- [µmol/kg] (Calculated using CO2SYS)	16 [HCO3]- std dev [±]	17 [CO3]2- [µmol/kg] (Calculated using CO2SYS)	18 [CO3]2- std dev [±]	19 pH (Calculated using CO2SYS)	20 pH std dev [±]	21 Repl [#]	22 P. alata l [µm] (Microscopy)	23 P. alata l std dev [±]	24 P. alata diam [µm] (Microscopy)	25 P. alata diam std dev [±]	26 P. alata vol [µm³] (Microscopy)	27 P. alata vol std dev [±]	28 FV/FM (see reference(s))	29 alpha std dev [±]	30 Chl a/cell [pg/#] (Spectrofluorometry)	31 Chl a std dev [±]	32 NO3 upt rate/cell [fmol/#/day] (Auto-analyzer, Technicon Traa...)	33 NO3 upt rate std dev [±]	34 PO4 upt rate/cell [fmol/#/day] (Auto-analyzer, Technicon Traa...)	35 PO4 upt rate std dev [±]	36 Si upt rate/cell [pmol/#/day] (Auto-analyzer, Technicon Traa...)	37 Si upt std dev [±]	38 N/P	39 N/P std dev [±]	40 N/Si	41 Std dev [±] (N/Si ratio)	42 Si/P	43 Si/P std dev [±]	44 CSC flag (Calculated using seacarb afte...)	45 pH (Total scale, Calculated using...)	46 CO2 [µmol/kg] (Calculated using seacarb afte...)	47 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	48 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	49 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	50 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	51 Omega Arg (Calculated using seacarb afte...)	52 Omega Cal (Calculated using seacarb afte...)
Low CO2 HL	35.5	3	1	16:08	240	2518	4	2141	6	135	5	8	0	1875	11	258	6	8.47	0.01	97	219.9	54.2	28.5	2.0	142400	46100	0.67	0.02	97.1	6.600	108000	67000	12000	5000	157	69	8.33	1.54	0.66	0.12	12.73	1.22	15	8.47	7.46	133.90	133.34	1872.41	261.13	3.92	6.22
Intermediate CO2 HL	35.5	3	1	16:08	240	2520	4	2229	12	200	9	11	1	2016	18	202	6	8.33	0.02	111	257.4	68.7	27.9	2.3	159700	54300	0.69	0.03	99.1	14.600	124000	64000	14000	8000	195	95	9.46	2.28	0.62	0.05	15.01	3.60	15	8.34	11.00	197.55	196.73	2013.35	204.65	3.07	4.88
High CO2 HL	35.5	3	1	16:08	240	2528	3	2428	7	599	40	33	2	2307	10	88	5	7.91	0.03	121	223.7	54.4	27.8	2.0	137200	42900	0.68	0.03	104.9	12.400	144000	51000	17000	6000	225	83	8.66	1.19	0.64	0.03	13.47	1.32	15	7.92	32.91	591.06	588.60	2305.41	89.68	1.35	2.14
Low CO2 LL	35.5	3	1	16:08	40	2517	4	2167	6	152	6	8	0	1919	12	240	6	8.43	0.02	130	263.5	97.5	28.5	2.4	170700	73400	0.69	0.05	109.8	12.900	57000	23000	3000	2000	140	111	20.04	5.40	0.53	0.21	47.37	31.62	15	8.43	8.38	150.46	149.84	1915.43	243.19	3.65	5.79
Intermediate CO2 LL	35.5	3	1	16:08	40	2515	3	2266	3	246	6	14	0	2078	5	175	3	8.25	0.01	110	229.5	78.2	29.1	2.7	154100	59500	0.69	0.05	91.4	16.500	49000	22000	3000	3000	106	42	10.98	5.06	0.45	0.08	23.97	11.35	15	8.26	13.47	241.90	240.89	2075.01	177.52	2.67	4.23
High CO2 LL	35.5	3	1	16:08	40	2532		2407		506		28		2277		102		7.98		43	271.2	56.3	29.5	2.2	188000	54400	0.67	0.04	540.3		428000	367000	31000	31000	392	289	13.43	2.41	1.02	0.18	13.49	4.09	15	7.99	27.86	500.29	498.21	2275.88	103.26	1.55	2.46