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Heinemann, Agnes; Fietzke, Jan; Melzner, Frank; Böhm, Florian; Thomsen, Jörn; Garbe-Schönberg, Carl-Dieter; Eisenhauer, Anton (2012): Seawater carbonate chemistry and conditions of Mytilus edulis extracellular body fluids and shell composition in a pH-treatment experiment: Acid-base status, trace elements and delta11B, 2012. doi:10.1594/PANGAEA.778194,
Supplement to: Heinemann, A et al. (2012): Conditions of Mytilus edulis extracellular body fluids and shell composition in a pH-treatment experiment: Acid-base status, trace elements and d11B. Geochemistry, Geophysics, Geosystems, 13, Q01005, doi:10.1029/2011GC003790

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Abstract:
Mytilus edulis were cultured for 3 months under six different seawater pCO2 levels ranging from 380 to 4000 µatm. Specimen were taken from Kiel Fjord (Western Baltic Sea, Germany) which is a habitat with high and variable seawater pCO2 and related shifts in carbonate system speciation (e.g., low pH and low CaCO3 saturation state). Hemolymph (HL) and extrapallial fluid (EPF) samples were analyzed for pH and total dissolved inorganic carbon (CT) to calculate pCO2 and [HCO3]. A second experiment was conducted for 2 months with three different pCO2 levels (380, 1400 and 4000 µatm). Boron isotopes (delta11B) were investigated by LA-MC-ICP-MS (Laser Ablation-Multicollector-Inductively Coupled Plasma-Mass Spectrometry) in shell portions precipitated during experimental treatment time. Additionally, elemental ratios (B/Ca, Mg/Ca and Sr/Ca) in the EPF of specimen from the second experiment were measured via ICP-OES (Inductively Coupled Plasma-Optical Emission Spectrometry). Extracellular pH was not significantly different in HL and EPF but systematically lower than ambient water pH. This is due to high extracellular pCO2 values, a prerequisite for metabolic CO2 excretion. No accumulation of extracellular [HCO3] was measured. Elemental ratios (B/Ca, Mg/Ca and Sr/Ca) in the EPF increased slightly with pH which is in accordance with increasing growth and calcification rates at higher seawater pH values. Boron isotope ratios were highly variable between different individuals but also within single shells. This corresponds to a high individual variability in fluid B/Ca ratios and may be due to high boron concentrations in the organic parts of the shell. The mean delta11B value shows no trend with pH but appears to represent internal pH (EPF) rather than ambient water pH.
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1IdentificationIDFietzke, Jan
2Experimental treatmentExp trtmFietzke, Jan
3SalinitySalFietzke, JanConductivity meter (WTW, Weilheim, Gemany)
4Salinity, standard deviationSal std dev±Fietzke, Jan
5Temperature, waterTemp°CFietzke, JanConductivity meter (WTW, Weilheim, Gemany)
6Temperature, standard deviationT std dev±Fietzke, Jan
7pHpHFietzke, JanConductivity meter (WTW, Weilheim, Gemany)NBS scale
8pH, standard deviationpH std dev±Fietzke, Jan
9Alkalinity, totalATµmol/kgFietzke, JanMeasured
10Alkalinity, total, standard deviationAT std dev±Fietzke, Jan
11Carbon, inorganic, dissolvedDICµmol/kgFietzke, JanAutomated CO2 analyzer (CIBA-Corning 965, UK)
12Carbon, inorganic, dissolved, standard deviationDIC std dev±Fietzke, Jan
13Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmFietzke, JanCalculated using CO2SYS
14Carbon dioxide, partial pressure, standard deviationpCO2 std dev±Fietzke, Jan
15Calcite saturation stateOmega CalFietzke, JanCalculated using CO2SYS
16Calcite saturation state, standard deviationOmega Cal std dev±Fietzke, Jan
17Aragonite saturation stateOmega ArgFietzke, JanCalculated using CO2SYS
18Aragonite saturation state, standard deviationOmega Arg std dev±Fietzke, Jan
19Mytilus edulis, extrapallial fluid pHM. edulis pH (EPF)Fietzke, JanConductivity meter (WTW, Weilheim, Gemany)
20Mytilus edulis, extrapallial fluid pH, standard deviationM. edulis pH (EPF) std dev±Fietzke, Jan
21ReplicatesRepl#Fietzke, JanEPF pH
22Mytilus edulis, haemolymph, pHM. edulis pH (ha)Fietzke, JanConductivity meter (WTW, Weilheim, Gemany)
23Mytilus edulis, haemolymph, pH, standard deviationM. edulis pH (ha) std dev±Fietzke, Jan
24ReplicatesRepl#Fietzke, JanHL pH
25pHpHFietzke, JanOn sampling day in water
26Mytilus edulis, extrapallial fluid total carbonM. edulis DIC (EPF)mmol/lFietzke, JanAutomated CO2 analyzer (CIBA-Corning 965, UK)
27Mytilus edulis, extrapallial fluid total carbon, standard deviationM. edulis DIC (EPF) std dev±Fietzke, Jan
28ReplicatesRepl#Fietzke, JanEPF DIC
29Mytilus edulis, haemolymph, total dissolved inorganic carbonM. edulis DIC (ha)mmol/lFietzke, JanAutomated CO2 analyzer (CIBA-Corning 965, UK)
30Mytilus edulis, haemolymph, total dissolved inorganic carbon, standard deviationM. edulis DIC (ha) std dev±Fietzke, Jan
31ReplicatesRepl#Fietzke, JanHL DIC
32Carbon, inorganic, dissolvedDICµmol/lFietzke, JanAutomated CO2 analyzer (CIBA-Corning 965, UK)On sampling day in water
33Mytilus edulis, extrapallial fluid partial pressure of carbon dioxideM. edulis pCO2 (EPF)µatmFietzke, JanCalculated, see reference(s)
34Mytilus edulis, extrapallial fluid partial pressure of carbon dioxide, standard deviationM. edulis pCO2 (EPF) std dev±Fietzke, Jan
35ReplicatesRepl#Fietzke, JanEPF pCO2
36Mytilus edulis, haemolymph, partial pressure of carbon dioxideM. edulis pCO2 (ha)µatmFietzke, JanCalculated, see reference(s)
37Mytilus edulis, haemolymph, partial pressure of carbon dioxide, standard deviationM. edulis pCO2 (ha) std dev±Fietzke, Jan
38ReplicatesRepl#Fietzke, JanHL pCO2
39Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmFietzke, JanCalculated using CO2SYSOn sampling day in water
40Mytilus edulis, extrapallial fluid bicarbonateM. edulis HCO3 (EPF)mmol/lFietzke, JanCalculated, see reference(s)
41Mytilus edulis, extrapallial fluid bicarbonate, standard deviationM. edulis HCO3 (EPF) std dev±Fietzke, Jan
42ReplicatesRepl#Fietzke, JanEPF HCO3
43Mytilus edulis, haemolymph, bicarbonate ionM. edulis [HCO3]- (ha)mmol/lFietzke, JanCalculated, see reference(s)
44Mytilus edulis, haemolymph, bicarbonate ion, standard deviationM. edulis [HCO3]- (ha) std dev±Fietzke, Jan
45ReplicatesRepl#Fietzke, JanHL HCO3
46Bicarbonate[HCO3]-mmol/lFietzke, JanCalculated using CO2SYSOn sampling day in water
47Mytilus edulis, extrapallial fluid carbonate ionM. edulis CO3 (EPF)µmol/lFietzke, JanCalculated, see reference(s)
48Mytilus edulis, extrapallial fluid carbonate ion, standard deviationM. edulis CO3 (EPF) std dev±Fietzke, Jan
49ReplicatesRepl#Fietzke, JanEPF CO3
50Mytilus edulis, haemolymph, carbonate ionM. edulis [CO3]2- (ha)µmol/lFietzke, JanCalculated, see reference(s)
51Mytilus edulis, haemolymph, carbonate ion, standard deviationM. edulis [CO3]2- (ha) std dev±Fietzke, Jan
52ReplicatesRepl#Fietzke, JanHL CO3
53Carbonate ion[CO3]2-µmol/lFietzke, JanCalculated using CO2SYSOn sampling day in water
54Mytilus edulis, extrapallial fluid pHM. edulis pH (EPF)Fietzke, JanConductivity meter (WTW, Weilheim, Gemany)
55Mytilus edulis, shell mass growthM. edulis Wµ%Fietzke, Jan
56Mytilus edulis, extrapallial fluid, boron/calcium ratioM. edulis B/Ca (EPF)mmol/molFietzke, JanICP-OES, Inductively coupled plasma - optical emission spectrometry
57Mytilus edulis, extrapallial fluid, boron/calcium ratio, standard deviationM. edulis B/Ca (EPF) std dev±Fietzke, Jan
58ReplicatesRepl#Fietzke, JanEPF B/Ca
59Boron/Calcium ratioB/Caµmol/molFietzke, JanICP-OES, Inductively coupled plasma - optical emission spectrometryOn sampling day in water
60Boron/Calcium ratio, standard deviationB/Ca std dev±Fietzke, Jan
61ReplicatesRepl#Fietzke, JanWater B/Ca
62Mytilus edulis, extrapallial fluid, strontium/calcium ratioM. edulis Sr/Ca (EPF)mmol/molFietzke, JanICP-OES, Inductively coupled plasma - optical emission spectrometry
63Mytilus edulis, extrapallial fluid, strontium/calcium ratio, standard deviationM. edulis Sr/Ca (EPF) std dev±Fietzke, Jan
64ReplicatesRepl#Fietzke, JanEPF Sr/Ca
65Strontium/Calcium ratioSr/Cammol/molFietzke, JanICP-OES, Inductively coupled plasma - optical emission spectrometryOn sampling day in water
66Strontium/Calcium ratio, standard deviationSr/Ca std dev±Fietzke, Jan
67ReplicatesRepl#Fietzke, JanWater Sr/Ca
68Mytilus edulis, extrapallial fluid, magnesium/calcium ratioM. edulis Mg/Ca (EPF)mmol/molFietzke, JanICP-OES, Inductively coupled plasma - optical emission spectrometry
69Mytilus edulis, extrapallial fluid, magnesium/calcium ratio, standard deviationM. edulis Mg/Ca (EPF) std dev±Fietzke, Jan
70ReplicatesRepl#Fietzke, JanEPF Mg/Ca
71Magnesium/Calcium ratioMg/Cammol/molFietzke, JanICP-OES, Inductively coupled plasma - optical emission spectrometryOn sampling day in water
72Magnesium/Calcium ratio, standard deviationMg/Ca std dev±Fietzke, Jan
73ReplicatesRepl#Fietzke, JanWater Mg/Ca
74Mytilus edulis, shell, d11BM. edulis d11Bper milFietzke, Jan
75Mytilus edulis, shell, d11B, standard deviationM. edulis d11B std dev±Fietzke, Jan
76ReplicatesRepl#Fietzke, Jandelta11B
77Mytilus edulis, length shellM. edulis L shellmmFietzke, Jan
78Mytilus edulis, shell length, standard deviationM. edulis shell l std dev±Fietzke, Jan
79ReplicatesRepl#Fietzke, JanM. edulis L
80Experimental treatmentExp trtmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
81pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale
82Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
83Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
84Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
85Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
86Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
87Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
88Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
Size:
531 data points

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