Data Description

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Citation:
Heinemann, A et al. (2012): Seawater carbonate chemistry and conditions of Mytilus edulis extracellular body fluids and shell composition in a pH-treatment experiment: Acid-base status, trace elements and delta11B, 2012. doi:10.1594/PANGAEA.778194,
Supplement to: Heinemann, Agnes; Fietzke, Jan; Melzner, Frank; Böhm, Florian; Thomsen, Jörn; Garbe-Schönberg, Carl-Dieter; Eisenhauer, Anton (2012): Conditions of Mytilus edulis extracellular body fluids and shell composition in a pH-treatment experiment: Acid-base status, trace elements and d11B. Geochemistry, Geophysics, Geosystems, 13, Q01005, doi:10.1029/2011GC003790
Abstract:
Mytilus edulis were cultured for 3 months under six different seawater pCO2 levels ranging from 380 to 4000 µatm. Specimen were taken from Kiel Fjord (Western Baltic Sea, Germany) which is a habitat with high and variable seawater pCO2 and related shifts in carbonate system speciation (e.g., low pH and low CaCO3 saturation state). Hemolymph (HL) and extrapallial fluid (EPF) samples were analyzed for pH and total dissolved inorganic carbon (CT) to calculate pCO2 and [HCO3]. A second experiment was conducted for 2 months with three different pCO2 levels (380, 1400 and 4000 µatm). Boron isotopes (delta11B) were investigated by LA-MC-ICP-MS (Laser Ablation-Multicollector-Inductively Coupled Plasma-Mass Spectrometry) in shell portions precipitated during experimental treatment time. Additionally, elemental ratios (B/Ca, Mg/Ca and Sr/Ca) in the EPF of specimen from the second experiment were measured via ICP-OES (Inductively Coupled Plasma-Optical Emission Spectrometry). Extracellular pH was not significantly different in HL and EPF but systematically lower than ambient water pH. This is due to high extracellular pCO2 values, a prerequisite for metabolic CO2 excretion. No accumulation of extracellular [HCO3] was measured. Elemental ratios (B/Ca, Mg/Ca and Sr/Ca) in the EPF increased slightly with pH which is in accordance with increasing growth and calcification rates at higher seawater pH values. Boron isotope ratios were highly variable between different individuals but also within single shells. This corresponds to a high individual variability in fluid B/Ca ratios and may be due to high boron concentrations in the organic parts of the shell. The mean delta11B value shows no trend with pH but appears to represent internal pH (EPF) rather than ambient water pH.
Project(s):
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1Identification *IDFietzke, Jan *
2Experimental treatment *Exp trtmFietzke, Jan *
3Salinity *SalFietzke, Jan *Conductivity meter (WTW, Weilheim, Gemany) *
4Salinity, standard deviation *Sal std dev±Fietzke, Jan *
5Temperature, water *Temp°CFietzke, Jan *Conductivity meter (WTW, Weilheim, Gemany) *
6Temperature, standard deviation *T std dev±Fietzke, Jan *
7pH *pHFietzke, Jan *Conductivity meter (WTW, Weilheim, Gemany) *NBS scale
8pH, standard deviation *pH std dev±Fietzke, Jan *
9Alkalinity, total *ATµmol/kgFietzke, Jan *Measured *
10Alkalinity, total, standard deviation *AT std dev±Fietzke, Jan *
11Carbon, inorganic, dissolved *DICµmol/kgFietzke, Jan *Automated CO2 analyzer (CIBA-Corning 965, UK) *
12Carbon, inorganic, dissolved, standard deviation *DIC std dev±Fietzke, Jan *
13Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmFietzke, Jan *Calculated using CO2SYS *
14Carbon dioxide, partial pressure, standard deviation *pCO2 std dev±Fietzke, Jan *
15Calcite saturation state *Omega CalFietzke, Jan *Calculated using CO2SYS *
16Calcite saturation state, standard deviation *Omega Cal std dev±Fietzke, Jan *
17Aragonite saturation state *Omega ArgFietzke, Jan *Calculated using CO2SYS *
18Aragonite saturation state, standard deviation *Omega Arg std dev±Fietzke, Jan *
19Mytilus edulis, extrapallial fluid pH *M. edulis pH (EPF)Fietzke, Jan *Conductivity meter (WTW, Weilheim, Gemany) *
20Mytilus edulis, extrapallial fluid pH, standard deviation *M. edulis pH (EPF) std dev±Fietzke, Jan *
21Replicates *Repl#Fietzke, Jan *EPF pH
22Mytilus edulis, haemolymph, pH *M. edulis pH (ha)Fietzke, Jan *Conductivity meter (WTW, Weilheim, Gemany) *
23Mytilus edulis, haemolymph, pH, standard deviation *M. edulis pH (ha) std dev±Fietzke, Jan *
24Replicates *Repl#Fietzke, Jan *HL pH
25pH *pHFietzke, Jan *On sampling day in water
26Mytilus edulis, extrapallial fluid total carbon *M. edulis DIC (EPF)mmol/lFietzke, Jan *Automated CO2 analyzer (CIBA-Corning 965, UK) *
27Mytilus edulis, extrapallial fluid total carbon, standard deviation *M. edulis DIC (EPF) std dev±Fietzke, Jan *
28Replicates *Repl#Fietzke, Jan *EPF DIC
29Mytilus edulis, haemolymph, total dissolved inorganic carbon *M. edulis DIC (ha)mmol/lFietzke, Jan *Automated CO2 analyzer (CIBA-Corning 965, UK) *
30Mytilus edulis, haemolymph, total dissolved inorganic carbon, standard deviation *M. edulis DIC (ha) std dev±Fietzke, Jan *
31Replicates *Repl#Fietzke, Jan *HL DIC
32Carbon, inorganic, dissolved *DICµmol/lFietzke, Jan *Automated CO2 analyzer (CIBA-Corning 965, UK) *On sampling day in water
33Mytilus edulis, extrapallial fluid partial pressure of carbon dioxide *M. edulis pCO2 (EPF)µatmFietzke, Jan *Calculated, see reference(s) *
34Mytilus edulis, extrapallial fluid partial pressure of carbon dioxide, standard deviation *M. edulis pCO2 (EPF) std dev±Fietzke, Jan *
35Replicates *Repl#Fietzke, Jan *EPF pCO2
36Mytilus edulis, haemolymph, partial pressure of carbon dioxide *M. edulis pCO2 (ha)µatmFietzke, Jan *Calculated, see reference(s) *
37Mytilus edulis, haemolymph, partial pressure of carbon dioxide, standard deviation *M. edulis pCO2 (ha) std dev±Fietzke, Jan *
38Replicates *Repl#Fietzke, Jan *HL pCO2
39Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmFietzke, Jan *Calculated using CO2SYS *On sampling day in water
40Mytilus edulis, extrapallial fluid bicarbonate *M. edulis HCO3 (EPF)mmol/lFietzke, Jan *Calculated, see reference(s) *
41Mytilus edulis, extrapallial fluid bicarbonate, standard deviation *M. edulis HCO3 (EPF) std dev±Fietzke, Jan *
42Replicates *Repl#Fietzke, Jan *EPF HCO3
43Mytilus edulis, haemolymph, bicarbonate ion *M. edulis [HCO3]- (ha)mmol/lFietzke, Jan *Calculated, see reference(s) *
44Mytilus edulis, haemolymph, bicarbonate ion, standard deviation *M. edulis [HCO3]- (ha) std dev±Fietzke, Jan *
45Replicates *Repl#Fietzke, Jan *HL HCO3
46Bicarbonate *[HCO3]-mmol/lFietzke, Jan *Calculated using CO2SYS *On sampling day in water
47Mytilus edulis, extrapallial fluid carbonate ion *M. edulis CO3 (EPF)µmol/lFietzke, Jan *Calculated, see reference(s) *
48Mytilus edulis, extrapallial fluid carbonate ion, standard deviation *M. edulis CO3 (EPF) std dev±Fietzke, Jan *
49Replicates *Repl#Fietzke, Jan *EPF CO3
50Mytilus edulis, haemolymph, carbonate ion *M. edulis [CO3]2- (ha)µmol/lFietzke, Jan *Calculated, see reference(s) *
51Mytilus edulis, haemolymph, carbonate ion, standard deviation *M. edulis [CO3]2- (ha) std dev±Fietzke, Jan *
52Replicates *Repl#Fietzke, Jan *HL CO3
53Carbonate ion *[CO3]2-µmol/lFietzke, Jan *Calculated using CO2SYS *On sampling day in water
54Mytilus edulis, extrapallial fluid pH *M. edulis pH (EPF)Fietzke, Jan *Conductivity meter (WTW, Weilheim, Gemany) *
55Mytilus edulis, shell mass growth *M. edulis Wµ%Fietzke, Jan *
56Mytilus edulis, extrapallial fluid, boron/calcium ratio *M. edulis B/Ca (EPF)mmol/molFietzke, Jan *ICP-OES, Inductively coupled plasma - optical emission spectrometry *
57Mytilus edulis, extrapallial fluid, boron/calcium ratio, standard deviation *M. edulis B/Ca (EPF) std dev±Fietzke, Jan *
58Replicates *Repl#Fietzke, Jan *EPF B/Ca
59Boron/Calcium ratio *B/Caµmol/molFietzke, Jan *ICP-OES, Inductively coupled plasma - optical emission spectrometry *On sampling day in water
60Boron/Calcium ratio, standard deviation *B/Ca std dev±Fietzke, Jan *
61Replicates *Repl#Fietzke, Jan *Water B/Ca
62Mytilus edulis, extrapallial fluid, strontium/calcium ratio *M. edulis Sr/Ca (EPF)mmol/molFietzke, Jan *ICP-OES, Inductively coupled plasma - optical emission spectrometry *
63Mytilus edulis, extrapallial fluid, strontium/calcium ratio, standard deviation *M. edulis Sr/Ca (EPF) std dev±Fietzke, Jan *
64Replicates *Repl#Fietzke, Jan *EPF Sr/Ca
65Strontium/Calcium ratio *Sr/Cammol/molFietzke, Jan *ICP-OES, Inductively coupled plasma - optical emission spectrometry *On sampling day in water
66Strontium/Calcium, standard deviation *Sr/Ca std dev±Fietzke, Jan *
67Replicates *Repl#Fietzke, Jan *Water Sr/Ca
68Mytilus edulis, extrapallial fluid, magnesium/calcium ratio *M. edulis Mg/Ca (EPF)mmol/molFietzke, Jan *ICP-OES, Inductively coupled plasma - optical emission spectrometry *
69Mytilus edulis, extrapallial fluid, magnesium/calcium ratio, standard deviation *M. edulis Mg/Ca (EPF) std dev±Fietzke, Jan *
70Replicates *Repl#Fietzke, Jan *EPF Mg/Ca
71Magnesium/Calcium ratio *Mg/Cammol/molFietzke, Jan *ICP-OES, Inductively coupled plasma - optical emission spectrometry *On sampling day in water
72Magnesium/Calcium ratio, standard deviation *Mg/Ca std dev±Fietzke, Jan *
73Replicates *Repl#Fietzke, Jan *Water Mg/Ca
74Mytilus edulis, shell, d11B *M. edulis d11Bper milFietzke, Jan *
75Mytilus edulis, shell, d11B, standard deviation *M. edulis d11B std dev±Fietzke, Jan *
76Replicates *Repl#Fietzke, Jan *delta11B
77Mytilus edulis, length shell *M. edulis L shellmmFietzke, Jan *
78Mytilus edulis, shell length, standard deviation *M. edulis shell l std dev±Fietzke, Jan *
79Replicates *Repl#Fietzke, Jan *M. edulis L
80Experimental treatment *Exp trtmNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
81pH *pHNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *Total scale
82Carbon dioxide *CO2µmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
83Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
84Fugacity of carbon dioxide (water) at sea surface temperature (wet air) *fCO2water_SST_wetµatmNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
85Bicarbonate ion *[HCO3]-µmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
86Carbonate ion *[CO3]2-µmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
87Aragonite saturation state *Omega ArgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
88Calcite saturation state *Omega CalNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
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