Heinemann, Agnes; Fietzke, Jan; Melzner, Frank; Böhm, Florian; Thomsen, Jörn; Garbe-Schönberg, Dieter; Eisenhauer, Anton (2012): Seawater carbonate chemistry and conditions of Mytilus edulis extracellular body fluids and shell composition in a pH-treatment experiment: Acid-base status, trace elements and delta11B, 2012 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.778194, Supplement to: Heinemann, A et al. (2012): Conditions of Mytilus edulis extracellular body fluids and shell composition in a pH-treatment experiment: Acid-base status, trace elements and d11B. Geochemistry, Geophysics, Geosystems, 13, Q01005, https://doi.org/10.1029/2011GC003790
Always quote citation above when using data! You can download the citation in several formats below.
Abstract:
Mytilus edulis were cultured for 3 months under six different seawater pCO2 levels ranging from 380 to 4000 µatm. Specimen were taken from Kiel Fjord (Western Baltic Sea, Germany) which is a habitat with high and variable seawater pCO2 and related shifts in carbonate system speciation (e.g., low pH and low CaCO3 saturation state). Hemolymph (HL) and extrapallial fluid (EPF) samples were analyzed for pH and total dissolved inorganic carbon (CT) to calculate pCO2 and [HCO3]. A second experiment was conducted for 2 months with three different pCO2 levels (380, 1400 and 4000 µatm). Boron isotopes (delta11B) were investigated by LA-MC-ICP-MS (Laser Ablation-Multicollector-Inductively Coupled Plasma-Mass Spectrometry) in shell portions precipitated during experimental treatment time. Additionally, elemental ratios (B/Ca, Mg/Ca and Sr/Ca) in the EPF of specimen from the second experiment were measured via ICP-OES (Inductively Coupled Plasma-Optical Emission Spectrometry). Extracellular pH was not significantly different in HL and EPF but systematically lower than ambient water pH. This is due to high extracellular pCO2 values, a prerequisite for metabolic CO2 excretion. No accumulation of extracellular [HCO3] was measured. Elemental ratios (B/Ca, Mg/Ca and Sr/Ca) in the EPF increased slightly with pH which is in accordance with increasing growth and calcification rates at higher seawater pH values. Boron isotope ratios were highly variable between different individuals but also within single shells. This corresponds to a high individual variability in fluid B/Ca ratios and may be due to high boron concentrations in the organic parts of the shell. The mean delta11B value shows no trend with pH but appears to represent internal pH (EPF) rather than ambient water pH.
Keyword(s):
Acid-base regulation; Animalia; Baltic Sea; Benthic animals; Benthos; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Mollusca; Mytilus edulis; Single species; Temperate
Project(s):
Funding:
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
531 data points
Data
1 ID | 2 Exp treat | 3 Sal (Conductivity meter (WTW, Weil...) | 4 Sal std dev [±] | 5 Temp [°C] (Conductivity meter (WTW, Weil...) | 6 T std dev [±] | 7 pH (NBS scale, Conductivity meter...) | 8 pH std dev [±] | 9 AT [µmol/kg] (Measured) | 10 AT std dev [±] | 11 DIC [µmol/kg] (Automated CO2 analyzer (CIBA-...) | 12 DIC std dev [±] | 13 pCO2water_SST_wet [µatm] (Calculated using CO2SYS) | 14 pCO2 std dev [±] | 15 Omega Cal (Calculated using CO2SYS) | 16 Omega Cal std dev [±] | 17 Omega Arg (Calculated using CO2SYS) | 18 Omega Arg std dev [±] | 19 M. edulis pH (epf) (Conductivity meter (WTW, Weil...) | 20 M. edulis pH (epf) std dev [±] | 21 Repl [#] (EPF pH) | 22 M. edulis pH (ha) (Conductivity meter (WTW, Weil...) | 23 M. edulis pH (ha) std dev [±] | 24 Repl [#] (HL pH) | 25 pH (On sampling day in water) | 26 M. edulis DIC (epf) [mmol/l] (Automated CO2 analyzer (CIBA-...) | 27 M. edulis DIC (epf) std dev [±] | 28 Repl [#] (EPF DIC) | 29 M. edulis DIC (ha) [mmol/l] (Automated CO2 analyzer (CIBA-...) | 30 M. edulis DIC (ha) std dev [±] | 31 Repl [#] (HL DIC) | 32 DIC [µmol/l] (On sampling day in water, Aut...) | 33 M. edulis pCO2 (epf) [µatm] (Calculated, see reference(s)) | 34 M. edulis pCO2 (epf) std dev [±] | 35 Repl [#] (EPF pCO2) | 36 M. edulis pCO2 (ha) [µatm] (Calculated, see reference(s)) | 37 M. edulis pCO2 (ha) std dev [±] | 38 Repl [#] (HL pCO2) | 39 pCO2water_SST_wet [µatm] (On sampling day in water, Cal...) | 40 M. edulis HCO3 (epf) [mmol/l] (Calculated, see reference(s)) | 41 M. edulis HCO3 (epf) std dev [±] | 42 Repl [#] (EPF HCO3) | 43 M. edulis [HCO3]- (ha) [mmol/l] (Calculated, see reference(s)) | 44 M. edulis [HCO3]- (ha) std dev [±] | 45 Repl [#] (HL HCO3) | 46 [HCO3]- [µmol/l] (On sampling day in water) | 47 M. edulis CO3 (epf) [µmol/l] (Calculated, see reference(s)) | 48 M. edulis CO3 (epf) std dev [±] | 49 Repl [#] (EPF CO3) | 50 M. edulis [CO3]2- (ha) [µmol/l] (Calculated, see reference(s)) | 51 M. edulis [CO3]2- (ha) std dev [±] | 52 Repl [#] (HL CO3) | 53 [CO3]2- [µmol/l] (On sampling day in water, Cal...) | 54 M. edulis pH (epf) (Conductivity meter (WTW, Weil...) | 55 M. edulis Wµ [%] | 56 M. edulis B/Ca (epf) [mmol/mol] (ICP-OES, Inductively coupled ...) | 57 M. edulis B/Ca (epf) std dev [±] | 58 Repl [#] (EPF B/Ca) | 59 B/Ca [µmol/mol] (On sampling day in water, ICP...) | 60 B/Ca std dev [±] | 61 Repl [#] (Water B/Ca) | 62 M. edulis Sr/Ca (epf) [mmol/mol] (ICP-OES, Inductively coupled ...) | 63 M. edulis Sr/Ca (epf) std dev [±] | 64 Repl [#] (EPF Sr/Ca) | 65 Sr/Ca [mmol/mol] (On sampling day in water, ICP...) | 66 Sr/Ca std dev [±] | 67 Repl [#] (Water Sr/Ca) | 68 M. edulis Mg/Ca (epf) [mmol/mol] (ICP-OES, Inductively coupled ...) | 69 M. edulis Mg/Ca (epf) std dev [±] | 70 Repl [#] (EPF Mg/Ca) | 71 Mg/Ca [mmol/mol] (On sampling day in water, ICP...) | 72 Mg/Ca std dev [±] | 73 Repl [#] (Water Mg/Ca) | 74 M. edulis δ11B [‰] | 75 M. edulis δ11B std dev [±] | 76 Repl [#] (delta11B) | 77 M. edulis shell L [mm] | 78 M. edulis sl std dev [±] | 79 Repl [#] (M. edulis L) | 80 Exp treat (Calculated using seacarb afte...) | 81 pH (Total scale, Calculated using...) | 82 CO2 [µmol/kg] (Calculated using seacarb afte...) | 83 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...) | 84 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...) | 85 [HCO3]- [µmol/kg] (Calculated using seacarb afte...) | 86 [CO3]2- [µmol/kg] (Calculated using seacarb afte...) | 87 Omega Arg (Calculated using seacarb afte...) | 88 Omega Cal (Calculated using seacarb afte...) |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Experiment 1 | pCO2=387 | 18.8 | 1.6 | 12.1 | 0.6 | 8.02 | 0.08 | 2031 | 74 | 1965 | 63 | 577 | 95 | 1.80 | 0.30 | 1.10 | 0.20 | 7.52 | 0.04 | 3 | 7.54 | 0.05 | 3 | 8.10 | 1.79 | 0.18 | 3 | 1.63 | 0.31 | 3 | 1960 | 1938 | 93 | 3 | 1648 | 183 | 3 | 452 | 1.70 | 0.18 | 3 | 1.55 | 0.30 | 3 | 1850 | 0.028 | 0.005 | 3 | 0.027 | 0.009 | 3 | 85.00 | 15 | 7.92 | 25.40 | 567.11 | 565.01 | 1872.22 | 67.39 | 1.05 | 1.76 | ||||||||||||||||||||||||||
Experiment 1 | pCO2=560 | 18.8 | 1.6 | 12.1 | 0.6 | 7.93 | 0.07 | 2031 | 74 | 1988 | 62 | 722 | 97 | 1.50 | 0.20 | 0.90 | 0.10 | 7.50 | 0.09 | 4 | 7.53 | 0.05 | 3 | 8.01 | 1.41 | 0.03 | 4 | 1.62 | 0.24 | 3 | 1980 | 1717 | 427 | 4 | 1623 | 190 | 3 | 574 | 1.33 | 0.04 | 4 | 1.54 | 0.23 | 3 | 1890 | 0.020 | 0.005 | 4 | 0.027 | 0.005 | 3 | 70.00 | 15 | 7.83 | 31.95 | 713.52 | 710.88 | 1900.84 | 55.21 | 0.86 | 1.44 | ||||||||||||||||||||||||||
Experiment 1 | pCO2=840 | 18.8 | 1.6 | 12.1 | 0.6 | 7.86 | 0.05 | 2032 | 72 | 2004 | 65 | 839 | 80 | 1.30 | 0.20 | 0.80 | 0.10 | 7.46 | 0.05 | 3 | 7.52 | 0.04 | 3 | 7.91 | 1.71 | 0.18 | 3 | 1.79 | 0.21 | 3 | 2010 | 2297 | 284 | 3 | 1987 | 383 | 3 | 731 | 1.61 | 0.17 | 3 | 1.70 | 0.19 | 3 | 1920 | 0.022 | 0.004 | 3 | 0.027 | 0.003 | 3 | 56.00 | 15 | 7.77 | 37.35 | 834.03 | 830.95 | 1918.53 | 48.12 | 0.75 | 1.26 | ||||||||||||||||||||||||||
Experiment 1 | pCO2=1120 | 18.8 | 1.6 | 12.1 | 0.6 | 7.77 | 0.05 | 2033 | 73 | 2026 | 66 | 1041 | 93 | 1.10 | 0.10 | 0.50 | 0.10 | 7.45 | 0.02 | 3 | 7.47 | 0.05 | 4 | 7.82 | 1.55 | 0.07 | 3 | 1.77 | 0.14 | 4 | 2030 | 2033 | 169 | 3 | 2226 | 348 | 4 | 906 | 1.46 | 0.07 | 3 | 1.67 | 0.13 | 4 | 1940 | 0.020 | 0.001 | 3 | 0.024 | 0.003 | 4 | 47.00 | 15 | 7.68 | 46.59 | 1040.40 | 1036.55 | 1939.97 | 39.44 | 0.62 | 1.03 | ||||||||||||||||||||||||||
Experiment 1 | pCO2=1400 | 18.8 | 1.6 | 12.1 | 0.6 | 7.71 | 0.05 | 2033 | 73 | 2040 | 66 | 1195 | 98 | 0.90 | 0.10 | 0.60 | 0.10 | 7.41 | 0.04 | 4 | 7.47 | 0.07 | 4 | 7.73 | 1.85 | 0.05 | 3 | 1.61 | 0.34 | 4 | 2050 | 2611 | 181 | 4 | 2199 | 744 | 4 | 1123 | 1.73 | 0.06 | 4 | 1.52 | 0.31 | 4 | 1960 | 0.023 | 0.003 | 4 | 0.021 | 0.003 | 4 | 38.00 | 15 | 7.62 | 53.90 | 1203.55 | 1199.10 | 1951.60 | 34.50 | 0.54 | 0.90 | ||||||||||||||||||||||||||
Experiment 1 | pCO2=4000 | 18.8 | 1.6 | 12.1 | 0.6 | 7.28 | 0.08 | 2040 | 72 | 2172 | 66 | 3352 | 566 | 0.40 | 0.10 | 0.20 | 0.04 | 7.34 | 0.09 | 4 | 7.37 | 0.02 | 4 | 7.39 | 1.91 | 0.07 | 4 | 1.89 | 0.31 | 4 | 2150 | 3430 | 696 | 4 | 3122 | 578 | 4 | 2724 | 1.77 | 0.08 | 4 | 1.75 | 0.28 | 4 | 2020 | 0.019 | 0.004 | 4 | 0.019 | 0.003 | 4 | 17.00 | 15 | 7.18 | 149.84 | 3345.94 | 3333.57 | 2009.01 | 13.15 | 0.21 | 0.34 | ||||||||||||||||||||||||||
Experiment 2 | pCO2=387 | 18.8 | 1.6 | 12.1 | 0.6 | 8.13 | 0.02 | 1966 | 3 | 1891 | 5 | 500 | 30 | 1.94 | 0.04 | 1.14 | 0.04 | 7.546 | 242 | 56.20 | 1.08 | 5 | 0.0543 | 0.0015 | 3 | 8.10 | 0.09 | 6 | 8.09 | 0.05 | 3 | 4947 | 54 | 6 | 4859 | 22 | 3 | 17.4 | 3.1 | 10 | 22.9 | 2.4 | 10 | 15 | 7.97 | 22.20 | 495.73 | 493.90 | 1797.72 | 71.08 | 1.11 | 1.86 | |||||||||||||||||||||||||||||||||||
Experiment 2 | pCO2=1400 | 18.8 | 1.6 | 12.1 | 0.6 | 7.72 | 0.06 | 1968 | 5 | 1984 | 12 | 1350 | 200 | 0.81 | 0.09 | 0.48 | 0.06 | 7.396 | 236 | 55.00 | 3.56 | 5 | 0.0539 | 0.0037 | 4 | 8.05 | 0.12 | 5 | 8.13 | 0.04 | 4 | 4878 | 125 | 5 | 4974 | 124 | 4 | 18.3 | 4.8 | 11 | 24.0 | 1.4 | 11 | 15 | 7.57 | 57.71 | 1288.61 | 1283.84 | 1895.88 | 30.41 | 0.48 | 0.79 | |||||||||||||||||||||||||||||||||||
Experiment 2 | pCO2=4000 | 18.8 | 1.6 | 12.1 | 0.6 | 7.26 | 0.04 | 1970 | 4 | 2126 | 13 | 3950 | 220 | 0.28 | 0.02 | 0.17 | 0.01 | 7.221 | 158 | 52.70 | 2.71 | 6 | 0.0531 | 0.0021 | 4 | 8.06 | 0.08 | 6 | 8.13 | 0.12 | 4 | 4817 | 62 | 6 | 4898 | 116 | 4 | 17.9 | 5.5 | 11 | 20.7 | 1.9 | 11 | 15 | 7.11 | 170.78 | 3813.56 | 3799.46 | 1944.41 | 10.81 | 0.17 | 0.28 |