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Levitan, Orly; Kranz, Sven A; Spungin, D; Prasil, O; Rost, Bjoern; Beran-Frank, Ilana (2010): Seawater carbonate chemistry and combined mechanistic effects of CO2 and light on the N2-fixing cyanobacterium Trichodesmium IMS101, 2010. PANGAEA,, Supplement to: Levitan, O et al. (2010): Combined effects of CO2 and light on the N2-fixing cyanobacterium Trichodesmium IMS101: A mechanistic view. Plant Physiology, 154, 346-356,

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The marine diazotrophic cyanobacterium Trichodesmium responds to elevated atmospheric CO2 partial pressure (pCO2) with higher N2 fixation and growth rates. To unveil the underlying mechanisms, we examined the combined influence of pCO2(150 and 900 µatm) and light (50 and 200 µmol photons m-2 s-1) on TrichodesmiumIMS101. We expand on a complementary study that demonstrated that while elevated pCO2 enhanced N2 fixation and growth, oxygen evolution and carbon fixation increased mainly as a response to high light. Here, we investigated changes in the photosynthetic fluorescence parameters of photosystem II, in ratios of the photosynthetic units (photosystem I:photosystem II), and in the pool sizes of key proteins involved in the fixation of carbon and nitrogen as well as their subsequent assimilation. We show that the combined elevation in pCO2 and light controlled the operation of the CO2-concentrating mechanism and enhanced protein activity without increasing their pool size. Moreover, elevated pCO2 and high light decreased the amounts of several key proteins (NifH, PsbA, and PsaC), while amounts of AtpB and RbcL did not significantly change. Reduced investment in protein biosynthesis, without notably changing photosynthetic fluxes, could free up energy that can be reallocated to increase N2 fixation and growth at elevated pCO2 and light. We suggest that changes in the redox state of the photosynthetic electron transportchain and posttranslational regulation of key proteins mediate the high flexibility in resources and energy allocation in Trichodesmium. This strategy should enableTrichodesmium to flourish in future surface oceans characterized by elevated pCO2, higher temperatures, and high light.
Bacteria; Bottles or small containers/Aquaria (<20 L); Cyanobacteria; Laboratory experiment; Laboratory strains; Light; Not applicable; Other metabolic rates; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Trichodesmium sp.
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1CommentCommentLevitan, Orly
2Time in hoursTimehLevitan, Orly
3Radiation, photosynthetically activePARµmol/m2/sLevitan, Orly
4Experimental treatmentExp treatLevitan, Orly
5Photosynthetic protein PsbAPsbApmol/µgLevitan, Orlysee reference(s)
6Photosynthetic protein PsbA standard deviationPsbA std dev±Levitan, Orly
7Photosynthetic protein PsbCPsaCpmol/µgLevitan, Orlysee reference(s)
8Photosynthetic protein PsbC standard deviationPsaC std dev±Levitan, Orly
9Photosynthetic protein RubiscoRbcLpmol/mgLevitan, Orlysee reference(s)
10Photosynthetic protein Rubisco, standard deviationRbcL std dev±Levitan, Orly
11CF1 subunit of ATP synthase proteinAtpBpmol/mgLevitan, Orlysee reference(s)
12CF1 subunit of ATP synthase protein, standard deviationAtpB std dev±Levitan, Orly
13Iron protein of nitrogenaseNifHpmol/mgLevitan, Orlysee reference(s)
14Iron protein of nitrogenase, standard deviationNifH std dev±Levitan, Orly
15GlnA subunit of Gln synthetaseGlnApmol/mgLevitan, Orlysee reference(s)
16GlnA subunit of Gln synthetase, standard deviationGlnA std dev±Levitan, Orly
17Fluorescence, intrinsicFoLevitan, Orlysee reference(s)
18Fluorescence, intrinsic, standard deviationFo std dev±Levitan, Orly
19Fluorescence, maximumFmLevitan, Orlysee reference(s)
20Fluorescence, maximum, standard deviationFm std dev±Levitan, Orly
21Fluorescence, variableFvLevitan, Orlysee reference(s)
22Fluorescence, variable, standard deviationFv std dev±Levitan, Orly
23Maximum photochemical quantum yield of photosystem IIFv/FmLevitan, Orlysee reference(s)
24Maximum photochemical quantum yield of photosystem II, standard deviationFv/Fm std dev±Levitan, Orly
25Effective absorbance cross-section of photosystem IIsigma PSIIA2/quantaLevitan, Orlysee reference(s)
26Effective absorbance cross-section of photosystem II, standard deviationsigma PSII std dev±Levitan, Orly
27Open photosystem II reaction centersPSIIopen#Levitan, Orlysee reference(s)
28Open photosystem II reaction centers, standard deviationPSIIopen std dev±Levitan, Orly
29Electron transport rate of photosystem II per cellETR PSII#/#/sLevitan, Orlysee reference(s)
30Electron transport rate of photosystem II, standard deviationETR PSII std dev±Levitan, Orly
31Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
32SalinitySalLevitan, Orly
33Temperature, waterTemp°CLevitan, Orly
34pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale
35Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
36Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
37Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
38Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
39Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
40Alkalinity, totalATµmol/kgLevitan, OrlyAlkalinity, Gran titration (Gran, 1950)
41Carbon, inorganic, dissolvedDICµmol/kgLevitan, OrlyCalculated using CO2SYS
42Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
43Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
608 data points

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