Citation:

pp.110.159285

Name: Seawater carbonate chemistry and combined mechanistic effects of CO2 and light on the N2-fixing cyanobacterium Trichodesmium IMS101, 2010
Published: 2010
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Bacteria; Bottles or small containers/Aquaria (<20 L); Cyanobacteria; Laboratory experiment; Laboratory strains; Light; Not applicable; Other metabolic rates; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Trichodesmium sp.

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Abstract:

The marine diazotrophic cyanobacterium Trichodesmium responds to elevated atmospheric CO2 partial pressure (pCO2) with higher N2 fixation and growth rates. To unveil the underlying mechanisms, we examined the combined influence of pCO2(150 and 900 µatm) and light (50 and 200 µmol photons m-2 s-1) on TrichodesmiumIMS101. We expand on a complementary study that demonstrated that while elevated pCO2 enhanced N2 fixation and growth, oxygen evolution and carbon fixation increased mainly as a response to high light. Here, we investigated changes in the photosynthetic fluorescence parameters of photosystem II, in ratios of the photosynthetic units (photosystem I:photosystem II), and in the pool sizes of key proteins involved in the fixation of carbon and nitrogen as well as their subsequent assimilation. We show that the combined elevation in pCO2 and light controlled the operation of the CO2-concentrating mechanism and enhanced protein activity without increasing their pool size. Moreover, elevated pCO2 and high light decreased the amounts of several key proteins (NifH, PsbA, and PsaC), while amounts of AtpB and RbcL did not significantly change. Reduced investment in protein biosynthesis, without notably changing photosynthetic fluxes, could free up energy that can be reallocated to increase N2 fixation and growth at elevated pCO2 and light. We suggest that changes in the redox state of the photosynthetic electron transportchain and posttranslational regulation of key proteins mediate the high flexibility in resources and energy allocation in Trichodesmium. This strategy should enableTrichodesmium to flourish in future surface oceans characterized by elevated pCO2, higher temperatures, and high light.

Keyword(s):

Bacteria; Bottles or small containers/Aquaria (<20 L); Cyanobacteria; Laboratory experiment; Laboratory strains; Light; Not applicable; Other metabolic rates; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Trichodesmium sp.

Project(s):

European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)

European Project on Ocean Acidification (EPOCA)

Ocean Acidification International Coordination Centre (OA-ICC)

Funding:

Seventh Framework Programme (FP7), grant/award no. 211384 : European Project on Ocean Acidification

Sixth Framework Programme (FP6), grant/award no. 511106 : European network of excellence for Ocean Ecosystems Analysis

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Comment	Comment		Levitan, Orly
2	Time in hours	Time	h	Levitan, Orly
3	Radiation, photosynthetically active	PAR	µmol/m²/s	Levitan, Orly
4	Experimental treatment	Exp treat		Levitan, Orly
5	Photosynthetic protein PsbA	PsbA	pmol/µg	Levitan, Orly	see reference(s)
6	Photosynthetic protein, PsbA, standard deviation	PsbA std dev	±	Levitan, Orly
7	Photosynthetic protein, PsbC	PsaC	pmol/µg	Levitan, Orly	see reference(s)
8	Photosynthetic protein, PsbC, standard deviation	PsaC std dev	±	Levitan, Orly
9	Photosynthetic protein Rubisco	RbcL	pmol/mg	Levitan, Orly	see reference(s)
10	Photosynthetic protein Rubisco, standard deviation	RbcL std dev	±	Levitan, Orly
11	CF1 subunit of ATP synthase protein	AtpB	pmol/mg	Levitan, Orly	see reference(s)
12	CF1 subunit of ATP synthase protein, standard deviation	AtpB std dev	±	Levitan, Orly
13	Iron protein of nitrogenase	NifH	pmol/mg	Levitan, Orly	see reference(s)
14	Iron protein of nitrogenase, standard deviation	NifH std dev	±	Levitan, Orly
15	GlnA subunit of Gln synthetase	GlnA	pmol/mg	Levitan, Orly	see reference(s)
16	GlnA subunit of Gln synthetase, standard deviation	GlnA std dev	±	Levitan, Orly
17	Fluorescence, intrinsic	Fo		Levitan, Orly	see reference(s)
18	Fluorescence, intrinsic, standard deviation	Fo std dev	±	Levitan, Orly
19	Fluorescence, maximum	Fm		Levitan, Orly	see reference(s)
20	Fluorescence, maximum, standard deviation	Fm std dev	±	Levitan, Orly
21	Fluorescence, variable	Fv		Levitan, Orly	see reference(s)
22	Fluorescence, variable, standard deviation	Fv std dev	±	Levitan, Orly
23	Maximum photochemical quantum yield of photosystem II	Fv/Fm		Levitan, Orly	see reference(s)
24	Maximum photochemical quantum yield of photosystem II, standard deviation	Fv/Fm std dev	±	Levitan, Orly
25	Effective absorbance cross-section of photosystem II	sigma PSII	A²/quanta	Levitan, Orly	see reference(s)
26	Effective absorbance cross-section of photosystem II, standard deviation	sigma PSII std dev	±	Levitan, Orly
27	Open photosystem II reaction centers	PSIIopen	#	Levitan, Orly	see reference(s)
28	Open photosystem II reaction centers, standard deviation	PSIIopen std dev	±	Levitan, Orly
29	Electron transport rate of photosystem II, per cell	ETR PSII/cell	#/#/s	Levitan, Orly	see reference(s)
30	Electron transport rate of photosystem II, standard deviation	ETR PSII std dev	±	Levitan, Orly
31	Carbonate system computation flag	CSC flag		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
32	Salinity	Sal		Levitan, Orly
33	Temperature, water	Temp	°C	Levitan, Orly
34	pH	pH		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)	Total scale
35	Carbon dioxide	CO2	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
36	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
37	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
38	Bicarbonate ion	[HCO3]-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
39	Carbonate ion	[CO3]2-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
40	Alkalinity, total	AT	µmol/kg	Levitan, Orly	Alkalinity, Gran titration (Gran, 1950)
41	Carbon, inorganic, dissolved	DIC	µmol/kg	Levitan, Orly	Calculated using CO2SYS
42	Aragonite saturation state	Omega Arg		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
43	Calcite saturation state	Omega Cal		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

608 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Comment	2 Time [h]	3 PAR [µmol/m²/s]	4 Exp treat	5 PsbA [pmol/µg]	6 PsbA std dev [±]	7 PsaC [pmol/µg]	8 PsaC std dev [±]	9 RbcL [pmol/mg]	10 RbcL std dev [±]	11 AtpB [pmol/mg]	12 AtpB std dev [±]	13 NifH [pmol/mg]	14 NifH std dev [±]	15 GlnA [pmol/mg]	16 GlnA std dev [±]	17 Fo	18 Fo std dev [±]	19 Fm	20 Fm std dev [±]	21 Fv	22 Fv std dev [±]	23 Fv/Fm	24 Fv/Fm std dev [±]	25 sigma PSII [A²/quanta]	26 sigma PSII std dev [±]	27 PSIIopen [#]	28 PSIIopen std dev [±]	29 ETR PSII/cell [#/#/s]	30 ETR PSII std dev [±]	31 CSC flag	32 Sal	33 Temp [°C]	34 pH	35 CO2 [µmol/kg]	36 pCO2water_SST_wet [µatm]	37 fCO2water_SST_wet [µatm]	38 [HCO3]- [µmol/kg]	39 [CO3]2- [µmol/kg]	40 AT [µmol/kg]	41 DIC [µmol/kg]	42 Omega Arg	43 Omega Cal
	1	50	pCO2=150	83	22	113	10	0.504	0.059	0.103	0.021	0.165	0.017	0.111	0.012															26	36	25	8.43	3.68	130.570	130.160	1432.600	431.020	2487.0	1867.3	6.79	10.28
	1	50	pCO2=900	65	21	136	10	0.600	0.085	0.114	0.023	0.163	0.032	0.097	0.012															26	36	25	7.80	23.08	819.700	817.120	2108.580	148.740	2470.0	2280.4	2.34	3.55
	1	200	pCO2=150	61	10	98	14	0.625	0.046	0.137	0.016	0.255	0.060	0.129	0.007															26	36	25	8.43	3.68	130.570	130.160	1432.600	431.020	2487.0	1867.3	6.79	10.28
	1	200	pCO2=900	40	0	92	16	0.553	0.072	0.116	0.057	0.173	0.025	0.107	0.014															26	36	25	7.80	23.08	819.700	817.120	2108.580	148.740	2470.0	2280.4	2.34	3.55
	5	50	pCO2=150	88	5	123	19	0.683	0.075	0.214	0.034	0.256	0.009	0.120	0.000															26	36	25	8.43	3.68	130.570	130.160	1432.600	431.020	2487.0	1867.3	6.79	10.28
	5	50	pCO2=900	75	12	120	10	0.709	0.065	0.142	0.010	0.270	0.057	0.090	0.024															26	36	25	7.80	23.08	819.700	817.120	2108.580	148.740	2470.0	2280.4	2.34	3.55
	5	200	pCO2=150	68	18	87	8	0.563	0.048	0.159	0.061	0.349	0.035	0.139	0.013															26	36	25	8.43	3.68	130.570	130.160	1432.600	431.020	2487.0	1867.3	6.79	10.28
	5	200	pCO2=900	56	17	84	5	0.529	0.054	0.128	0.035	0.167	0.025	0.108	0.004															26	36	25	7.80	23.08	819.700	817.120	2108.580	148.740	2470.0	2280.4	2.34	3.55
0	1	50	pCO2=150													209.9	16.0	361.7	40.1	151.8	30.5	0.42	0.04	244.1	9.0					26	36	25	8.43	3.68	130.570	130.160	1432.600	431.020	2487.0	1867.3	6.79	10.28
0	1	50	pCO2=900													183.4	58.4	286.6	86.8	103.2	29.7	0.36	0.03	247.5	18.4					26	36	25	7.80	23.08	819.700	817.120	2108.580	148.740	2470.0	2280.4	2.34	3.55
0	1	200	pCO2=150													262.1	97.0	350.1	151.0	131.6	55.6	0.38	0.04	206.0	2404.0					26	36	25	8.43	3.68	130.570	130.160	1432.600	431.020	2487.0	1867.3	6.79	10.28
0	1	200	pCO2=900													236.4	65.0	338.0	66.7	101.7	16.1	0.31	0.06	242.1	14.4					26	36	25	7.80	23.08	819.700	817.120	2108.580	148.740	2470.0	2280.4	2.34	3.55
0	5	50	pCO2=150													273.9	44.0	409.5	68.3	135.6	26.5	0.33	0.02	225.7	9.7					26	36	25	8.43	3.68	130.570	130.160	1432.600	431.020	2487.0	1867.3	6.79	10.28
0	5	50	pCO2=900													234.8	51.9	332.6	71.2	97.8	20.0	0.30	0.02	232.0	12.0					26	36	25	7.80	23.08	819.700	817.120	2108.580	148.740	2470.0	2280.4	2.34	3.55
0	5	200	pCO2=150													265.9	23.4	353.1	25.4	87.2	14.4	0.25	0.04	212.7	14.6					26	36	25	8.43	3.68	130.570	130.160	1432.600	431.020	2487.0	1867.3	6.79	10.28
0	5	200	pCO2=900													218.1	52.0	272.6	56.2	54.6	6.5	0.20	0.03	197.7	22.2					26	36	25	7.80	23.08	819.700	817.120	2108.580	148.740	2470.0	2280.4	2.34	3.55
acclimation	1	50	pCO2=150																							0.72	0.03	7.15	1.95	26	36	25	8.43	3.68	130.570	130.160	1432.600	431.020	2487.0	1867.3	6.79	10.28
acclimation	1	50	pCO2=900																							0.69	0.05	4.28	0.88	26	36	25	7.80	23.08	819.700	817.120	2108.580	148.740	2470.0	2280.4	2.34	3.55
acclimation	1	200	pCO2=150																							0.64	0.12	42.99	21.47	26	36	25	8.43	3.68	130.570	130.160	1432.600	431.020	2487.0	1867.3	6.79	10.28
acclimation	1	200	pCO2=900																							0.77	0.29	17.60	5.79	26	36	25	7.80	23.08	819.700	817.120	2108.580	148.740	2470.0	2280.4	2.34	3.55
acclimation	5	50	pCO2=150																							0.88	0.14	9.32	1.74	26	36	25	8.43	3.68	130.570	130.160	1432.600	431.020	2487.0	1867.3	6.79	10.28
acclimation	5	50	pCO2=900																							0.76	0.09	7.29	1.75	26	36	25	7.80	23.08	819.700	817.120	2108.580	148.740	2470.0	2280.4	2.34	3.55
acclimation	5	200	pCO2=150																							0.78	0.07	57.43	14.50	26	36	25	8.43	3.68	130.570	130.160	1432.600	431.020	2487.0	1867.3	6.79	10.28
acclimation	5	200	pCO2=900																							0.57	0.51	29.98	19.28	26	36	25	7.80	23.08	819.700	817.120	2108.580	148.740	2470.0	2280.4	2.34	3.55