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Piontek, Judith; Lunau, Mirko; Händel, Nicole; Borchard, Corinna; Wurst, Mascha; Engel, Anja (2010): Seawater carbonate chemistry and microbial polysaccharide degradation during experiments with phytoplankton Emiliania huxleyi (strain PML B92/11) and natural bacteria community, 2010. PANGAEA,, Supplement to: Piontek, J et al. (2010): Acidification increases microbial polysaccharide degradation in the ocean. Biogeosciences, 7(5), 1615-1625,

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With the accumulation of anthropogenic carbon dioxide (CO2), a proceeding decline in seawater pH has been induced that is referred to as ocean acidification. The ocean's capacity for CO2 storage is strongly affected by biological processes, whose feedback potential is difficult to evaluate. The main source of CO2 in the ocean is the decomposition and subsequent respiration of organic molecules by heterotrophic bacteria. However, very little is known about potential effects of ocean acidification on bacterial degradation activity. This study reveals that the degradation of polysaccharides, a major component of marine organic matter, by bacterial extracellular enzymes was significantly accelerated during experimental simulation of ocean acidification. Results were obtained from pH perturbation experiments, where rates of extracellular alpha- and beta-glucosidase were measured and the loss of neutral and acidic sugars from phytoplankton-derived polysaccharides was determined. Our study suggests that a faster bacterial turnover of polysaccharides at lowered ocean pH has the potential to reduce carbon export and to enhance the respiratory CO2 production in the future ocean.
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Experimental treatmentExp treatPiontek, Judith
2Sample IDSample IDPiontek, Judith
3Light:Dark cycleL:Dhh:hhPiontek, JudithMeasured
4Radiation, photosynthetically activePARµmol/m2/sPiontek, Judith
5Time, incubationT incubationdayPiontek, Judith
6Temperature, waterTemp°CPiontek, Judith
7pHpHPiontek, JudithWTW 340i pH-analyzer and WTW SenTix 81-electrodeNBS scale
8BacteriaBact#/mlPiontek, JudithFACSCalibur flow-cytometer (Becton Dicinson)
9Bacteria, abundance, standard deviationBact abund std dev±Piontek, Judith
10Carbon, organic, particulatePOCµmol/lPiontek, JudithElement analyser CNS, EURO EA
11Carbon, organic, particulate, standard deviationPOC std dev±Piontek, Judith
12Combined glucose lossGlucose loss%Piontek, Judith
13Combined glucose loss, standard deviationGlucose loss std dev±Piontek, Judith
14Combined glucose lossGlucose lossµmol/lPiontek, Judith
15Combined glucose loss, standard deviationGlucose loss std dev±Piontek, Judith
16Polysacchrides lossPolysacch loss%Piontek, JudithHigh Performance anion-exchange chromatography
17Polysacchrides loss, standard deviationPolysacch loss std dev±Piontek, Judith
18Polysacchrides lossPolysacch lossµmol/lPiontek, JudithHigh Performance anion-exchange chromatography
19Polysacchrides loss, standard deviationPolysacch loss std dev±Piontek, Judith
20Particulate organic carbon lossPOC loss%Piontek, JudithElement analyser CNS, EURO EA
21Particulate organic carbon loss, standard deviationPOC loss±Piontek, Judith
22Particulate organic carbon lossPOC lossµmol/lPiontek, JudithElement analyser CNS, EURO EA
23Particulate organic carbon loss, standard deviationPOC loss±Piontek, Judith
24Cell-specific glucosidase activityGLcellnmol/l/hPiontek, Judith
25Cell-specific glucosidase activity, standard deviationGLcell std dev±Piontek, Judith
26alpha-glucosidase activity per cellaGLamol/#dayPiontek, Judithsee reference(s)
27beta-glucosidase activity per cellbGLamol/#/dayPiontek, Judithsee reference(s)
452 data points

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