Citation:

bg-7-1615-2010

Name: Seawater carbonate chemistry and microbial polysaccharide degradation during experiments with phytoplankton Emiliania huxleyi (strain PML B92/11) and natural bacteria community, 2010
Published: 2010
License: https://creativecommons.org/licenses/by/3.0/

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Abstract:

With the accumulation of anthropogenic carbon dioxide (CO2), a proceeding decline in seawater pH has been induced that is referred to as ocean acidification. The ocean's capacity for CO2 storage is strongly affected by biological processes, whose feedback potential is difficult to evaluate. The main source of CO2 in the ocean is the decomposition and subsequent respiration of organic molecules by heterotrophic bacteria. However, very little is known about potential effects of ocean acidification on bacterial degradation activity. This study reveals that the degradation of polysaccharides, a major component of marine organic matter, by bacterial extracellular enzymes was significantly accelerated during experimental simulation of ocean acidification. Results were obtained from pH perturbation experiments, where rates of extracellular alpha- and beta-glucosidase were measured and the loss of neutral and acidic sugars from phytoplankton-derived polysaccharides was determined. Our study suggests that a faster bacterial turnover of polysaccharides at lowered ocean pH has the potential to reduce carbon export and to enhance the respiratory CO2 production in the future ocean.

Project(s):

European Project on Ocean Acidification (EPOCA)

Funding:

Seventh Framework Programme (FP7), grant/award no. 211384 : European Project on Ocean Acidification

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Experimental treatment	Exp treat		Piontek, Judith
2	Sample ID	Sample ID		Piontek, Judith
3	Light:Dark cycle	L:D	hh:hh	Piontek, Judith	Measured
4	Radiation, photosynthetically active	PAR	µmol/m²/s	Piontek, Judith
5	Time, incubation	T incubation	day	Piontek, Judith
6	Temperature, water	Temp	°C	Piontek, Judith
7	pH	pH		Piontek, Judith	WTW 340i pH-analyzer and WTW SenTix 81-electrode	NBS scale
8	Bacteria	Bact	#/ml	Piontek, Judith	FACSCalibur flow-cytometer (Becton Dickinson)
9	Bacteria, abundance, standard deviation	Bact abund std dev	±	Piontek, Judith
10	Carbon, organic, particulate	POC	µmol/l	Piontek, Judith	Element analyser CNS, EURO EA
11	Carbon, organic, particulate, standard deviation	POC std dev	±	Piontek, Judith
12	Combined glucose loss	Glc loss	%	Piontek, Judith
13	Combined glucose loss, standard deviation	Glc loss std dev	±	Piontek, Judith
14	Combined glucose loss	Glc loss	µmol/l	Piontek, Judith
15	Combined glucose loss, standard deviation	Glc loss std dev	±	Piontek, Judith
16	Polysacchrides loss	Polysacch loss	%	Piontek, Judith	High Performance anion-exchange chromatography
17	Polysacchrides loss, standard deviation	Polysacch loss std dev	±	Piontek, Judith
18	Polysacchrides loss	Polysacch loss	µmol/l	Piontek, Judith	High Performance anion-exchange chromatography
19	Polysacchrides loss, standard deviation	Polysacch loss std dev	±	Piontek, Judith
20	Particulate organic carbon loss	POC loss	%	Piontek, Judith	Element analyser CNS, EURO EA
21	Particulate organic carbon loss, standard deviation	POC loss	±	Piontek, Judith
22	Particulate organic carbon loss	POC loss	µmol/l	Piontek, Judith	Element analyser CNS, EURO EA
23	Particulate organic carbon loss, standard deviation	POC loss	±	Piontek, Judith
24	Cell-specific glucosidase activity	GLcell	nmol/l/h	Piontek, Judith
25	Cell-specific glucosidase activity, standard deviation	GLcell std dev	±	Piontek, Judith
26	alpha-glucosidase activity per cell	aGL/cell	amol/#day	Piontek, Judith	see reference(s)
27	beta-glucosidase activity per cell	bGL/cell	amol/#/day	Piontek, Judith	see reference(s)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Size:

452 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Exp treat	2 Sample ID	3 L:D [hh:hh] (Measured)	4 PAR [µmol/m²/s]	5 T incubation [day]	6 Temp [°C]	7 pH (NBS scale, WTW 340i pH-analyz...)	8 Bact [#/ml] (FACSCalibur flow-cytometer (B...)	9 Bact abund std dev [±]	10 POC [µmol/l] (Element analyser CNS, EURO EA)	11 POC std dev [±]	12 Glc loss [%]	13 Glc loss std dev [±]	14 Glc loss [µmol/l]	15 Glc loss std dev [±]	16 Polysacch loss [%] (High Performance anion-exchan...)	17 Polysacch loss std dev [±]	18 Polysacch loss [µmol/l] (High Performance anion-exchan...)	19 Polysacch loss std dev [±]	20 POC loss [%] (Element analyser CNS, EURO EA)	21 POC loss [±]	22 POC loss [µmol/l] (Element analyser CNS, EURO EA)	23 POC loss [±]	24 GLcell [nmol/l/h]	25 GLcell std dev [±]	26 aGL/cell [amol/#day] (see reference(s))	27 bGL/cell [amol/#/day] (see reference(s))
Present day	CultExp I	16:08	200	30	14	8.3	21400000	5300000	522	168	50.5	7.3			50.4	5.2			5.0	7.1			9.00	0.72	67.4	144.8
Present day	CultExp I	16:08	200	30	14	8.3	21400000	5300000	522	168															101.7	129.8
Present day	CultExp I	16:08	200	30	14	8.3	21400000	5300000	522	168															231.6	239.6
Present day	CultExp I	16:08	200	30	14	8.3	21400000	5300000	522	168															258.3	251.0
Present day	CultExp I	16:08	200	30	14	8.3	21400000	5300000	522	168															469.2	464.9
Future ocean	CultExp I	16:08	200	30	14	8.1	21400000	5300000	522	168	72.1	11.1			70.2	16.8			41.0	12.7			17.68	0.97	71.9	232.2
Future ocean	CultExp I	16:08	200	30	14	8.1	21400000	5300000	522	168															162.6	243.0
Future ocean	CultExp I	16:08	200	30	14	8.1	21400000	5300000	522	168															266.4	604.0
Future ocean	CultExp I	16:08	200	30	14	8.1	21400000	5300000	522	168															538.5	789.4
Future ocean	CultExp I	16:08	200	30	14	8.1	21400000	5300000	522	168															924.3	1763.7
Present day	CultExp II	16:08	300	13	15	7.9	4900000	1800000	840	86	87.0				59.0				67.0				2.81		20.5	48.4
Present day	CultExp II	16:08	300	13	15	7.9	4900000	1800000	840	86															28.6	66.1
Present day	CultExp II	16:08	300	13	15	7.9	4900000	1800000	840	86															17.2	39.4
Present day	CultExp II	16:08	300	13	15	7.9	4900000	1800000	840	86															15.4	36.1
Present day	CultExp II	16:08	300	13	15	7.9	4900000	1800000	840	86															5.1	27.9
Present day	CultExp II	16:08	300	13	15	7.9	4900000	1800000	840	86															4.4	20.7
Present day	CultExp II	16:08	300	13	15	7.9	4900000	1800000	840	86															6.2	26.2
Future ocean	CultExp II	16:08	300	13	15	7.7	4900000	1800000	840	86	95.0				73.0				74.0				12.11		106.9	229.7
Future ocean	CultExp II	16:08	300	13	15	7.7	4900000	1800000	840	86															110.7	252.7
Future ocean	CultExp II	16:08	300	13	15	7.7	4900000	1800000	840	86															107.0	206.7
Future ocean	CultExp II	16:08	300	13	15	7.7	4900000	1800000	840	86															78.5	141.5
Future ocean	CultExp II	16:08	300	13	15	7.7	4900000	1800000	840	86															43.8	159.8
Future ocean	CultExp II	16:08	300	13	15	7.7	4900000	1800000	840	86															36.8	88.5
Future ocean	CultExp II	16:08	300	13	15	7.7	4900000	1800000	840	86															88.3	177.5
Present day	FieldAssay I	16:08	200	12	10	8.2	80000		14		35.0				6.0								0.39	0.06	7.8	11.4
Present day	FieldAssay I	16:08	200	12	10	8.2	80000		14																17.6	11.9
Present day	FieldAssay I	16:08	200	12	10	8.2	80000		14																5.9	10.1
Future ocean	FieldAssay I	16:08	200	12	10	7.9	80000		14		67.0				32.0								0.77	0.28	11.1	49.9
Future ocean	FieldAssay I	16:08	200	12	10	7.9	80000		14																29.1	30.3
Future ocean	FieldAssay I	16:08	200	12	10	7.9	80000		14																11.0	46.6
Present day	FieldAssay II	16:08	200	8	16	7.9	6200000	2500000	48	12			5.6	0.9			7.0	1.1			56.6	3.2	17.68	1.06	26.8	69.6
Future ocean	FieldAssay II	16:08	200	8	16	7.6	6200000	2500000	48	12			3.0	1.7			4.4	1.5			40.2	15.4	1.61	5.03	719.2	924.0