Anderson, Kelli; Elizur, Abigail (2011): qPCR counts of mRNA for hepatic reference gene selection in juvenile female Atlantic salmon from perturbation experiments under artificial temperature conditions [dataset]. University of the Sunshine Coast, PANGAEA, https://doi.org/10.1594/PANGAEA.772213, In supplement to: Anderson, K; Elizur, A (2012): Hepatic reference gene selection in adult and juvenile female Atlantic salmon at normal and elevated temperatures. BMC Research Notes, 5(21), 1-9, https://doi.org/10.1186/1756-0500-5-21
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Abstract:
The dataset contains raw data (quantification cycle) for a study which determined the most suitable hepatic reference genes for normalisation of qPCR data orginating from juvenile Atlantic salmon (14 days) exposed to 14 and 22 degrees C. These results will be useful for anyone wanting to study the effects of climate change/elevated temperature on reproductive physiology of fish (and perhaphs other vertebrates).
Coverage:
Date/Time Start: 2009-01-02T00:00:00 * Date/Time End: 2009-01-02T00:00:00
Event(s):
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | ||||
| 2 | DATE/TIME | Date/Time | Geocode | |||
| 3 | Treatment: duration | T:duration | days | Anderson, Kelli | Duration of the treatment | |
| 4 | Treatment: hormones | T:hormones | Anderson, Kelli | Hormones used as treatment | ||
| 5 | Treatment: temperature | T:temp | °C | Anderson, Kelli | Temperature used as treatment | |
| 6 | Life Cycle: state | LC:state | Anderson, Kelli | State of the Life cycle | ||
| 7 | qPCR counts, mean | Cq | # | Anderson, Kelli | Fluorescence-based quantitative real-time PCR (qPCR) | of mRNA [vernacular: mRNA abundance] expressing gene Tbp [GenBank: BT059217] in liver of Salmo salar [WoRMS ID: 127186; vernacular: Atantic salmon] |
| 8 | qPCR counts, standard error of mean | Cq SEM | ± | Anderson, Kelli | Fluorescence-based quantitative real-time PCR (qPCR) | of mRNA [vernacular: mRNA abundance] expressing gene Tbp [GenBank: BT059217] in liver of Salmo salar [WoRMS ID: 127186; vernacular: Atantic salmon] |
| 9 | qPCR counts, mean | Cq | # | Anderson, Kelli | Fluorescence-based quantitative real-time PCR (qPCR) | of mRNA [vernacular: mRNA abundance] expressing gene Hprt1 [GenBank: BT043501] in liver of Salmo salar [WoRMS ID: 127186; vernacular: Atantic salmon] |
| 10 | qPCR counts, standard error of mean | Cq SEM | ± | Anderson, Kelli | Fluorescence-based quantitative real-time PCR (qPCR) | of mRNA [vernacular: mRNA abundance] expressing gene Hprt1 [GenBank: BT043501] in liver of Salmo salar [WoRMS ID: 127186; vernacular: Atantic salmon] |
| 11 | qPCR counts, mean | Cq | # | Anderson, Kelli | Fluorescence-based quantitative real-time PCR (qPCR) | of mRNA [vernacular: mRNA abundance] expressing gene Efla [GenBank: AF321836] in liver of Salmo salar [WoRMS ID: 127186; vernacular: Atantic salmon] |
| 12 | qPCR counts, standard error of mean | Cq SEM | ± | Anderson, Kelli | Fluorescence-based quantitative real-time PCR (qPCR) | of mRNA [vernacular: mRNA abundance] expressing gene Efla [GenBank: AF321836] in liver of Salmo salar [WoRMS ID: 127186; vernacular: Atantic salmon] |
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Size:
120 data points
Data
| 1 Event | 2 Date/Time | 3 T:duration [days] (Duration of the treatment) | 4 T:hormones (Hormones used as treatment) | 5 T:temp [°C] (Temperature used as treatment) | 6 LC:state (State of the Life cycle) | 7 Cq [#] (of mRNA [vernacular: mRNA abu...) | 8 Cq SEM [±] (of mRNA [vernacular: mRNA abu...) | 9 Cq [#] (of mRNA [vernacular: mRNA abu...) | 10 Cq SEM [±] (of mRNA [vernacular: mRNA abu...) | 11 Cq [#] (of mRNA [vernacular: mRNA abu...) | 12 Cq SEM [±] (of mRNA [vernacular: mRNA abu...) |
|---|---|---|---|---|---|---|---|---|---|---|---|
| SALTAS_Experimental_Tank(14degC)_February_2009 | 2009-01-02T00:00 | 3.00 | E2 silastic implant | 14.00 | female, juvenile | 26.47 | 0.95 | 19.73 | 0.63 | 14.43 | 0.39 |
| SALTAS_Experimental_Tank(14degC)_February_2009 | 2009-01-02T00:00 | 3.00 | Blank | 14.00 | female, juvenile | 27.67 | 0.75 | 19.93 | 0.89 | 14.13 | 0.33 |
| SALTAS_Experimental_Tank(14degC)_February_2009 | 2009-01-02T00:00 | 7.00 | E2 silastic implant | 14.00 | female, juvenile | 26.51 | 0.49 | 20.63 | 0.36 | 14.94 | 0.35 |
| SALTAS_Experimental_Tank(14degC)_February_2009 | 2009-01-02T00:00 | 7.00 | Blank | 14.00 | female, juvenile | 28.09 | 0.23 | 20.74 | 0.16 | 14.98 | 0.09 |
| SALTAS_Experimental_Tank(14degC)_February_2009 | 2009-01-02T00:00 | 14.00 | E2 silastic implant | 14.00 | female, juvenile | 26.88 | 0.34 | 20.87 | 0.31 | 14.97 | 0.24 |
| SALTAS_Experimental_Tank(14degC)_February_2009 | 2009-01-02T00:00 | 14.00 | Blank | 14.00 | female, juvenile | 26.82 | 0.31 | 20.42 | 0.26 | 14.91 | 0.27 |
| SALTAS_Experimental_Tank(22degC)_February_2009 | 2009-01-02T00:00 | 3.00 | E2 silastic implant | 22.00 | female, juvenile | 26.71 | 0.45 | 20.82 | 0.17 | 14.82 | 0.17 |
| SALTAS_Experimental_Tank(22degC)_February_2009 | 2009-01-02T00:00 | 3.00 | Blank | 22.00 | female, juvenile | 27.15 | 0.51 | 21.22 | 0.56 | 14.06 | 0.36 |
| SALTAS_Experimental_Tank(22degC)_February_2009 | 2009-01-02T00:00 | 7.00 | E2 silastic implant | 22.00 | female, juvenile | 27.85 | 0.12 | 21.26 | 0.23 | 14.92 | 0.36 |
| SALTAS_Experimental_Tank(22degC)_February_2009 | 2009-01-02T00:00 | 7.00 | Blank | 22.00 | female, juvenile | 26.82 | 0.26 | 20.62 | 0.34 | 14.23 | 0.25 |
| SALTAS_Experimental_Tank(22degC)_February_2009 | 2009-01-02T00:00 | 14.00 | E2 silastic implant | 22.00 | female, juvenile | 27.52 | 0.64 | 21.89 | 0.58 | 14.85 | 0.15 |
| SALTAS_Experimental_Tank(22degC)_February_2009 | 2009-01-02T00:00 | 14.00 | Blank | 22.00 | female, juvenile | 28.52 | 0.31 | 21.17 | 0.17 | 14.84 | 0.23 |