Data Description

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Citation:
Bach, LT et al. (2011): Seawater carbonate chemistry, growth rate and PIC and POC production during experiments with Emiliania huxleyi (B92/11), 2011. doi:10.1594/PANGAEA.771288,
Supplement to: Bach, Lennart Thomas; Riebesell, Ulf; Schulz, Kai Georg (2011): Distinguishing between the effects of ocean acidification and ocean carbonation in the coccolithophore Emiliania huxleyi. Limnology and Oceanography, 56(6), 2040-2050, doi:10.4319/lo.2011.56.6.2040
Abstract:
The coccolithophore Emiliania huxleyi was cultured under a broad range of carbonate chemistry conditions to distinguish the effects of individual carbonate system parameters on growth, primary production, and calcification. In the first experiment, alkalinity was kept constant and the fugacity of CO2(fCO2) varied from 2 to 600 Pa (1Pa ~ 10 µatm). In the second experiment, pH was kept constant (pHfree = 8) with fCO2 varying from 4 to 370 Pa. Results of the constant-alkalinity approach revealed physiological optima for growth, calcification, and organic carbon production at fCO2 values of ~20Pa, ~40 Pa, and ~80 Pa, respectively. Comparing this with the constant-pH approach showed that growth and organic carbon production increased similarly from low to intermediate CO2 levels but started to diverge towards higher CO2 levels. In the high CO2 range, growth rates and organic carbon production decreased steadily with declining pH at constant alkalinity while remaining consistently higher at constant pH. This suggests that growth and organic carbon production rates are directly related to CO2 at low (sub-saturating) concentrations, whereas towards higher CO2 levels they are adversely affected by the associated decrease in pH. A pH dependence at high fCO2 is also indicated for calcification rates, while the key carbonate system parameter determining calcification at low fCO2 remains unclear. These results imply that key metabolic processes in coccolithophores have their optima at different carbonate chemistry conditions and are influenced by different parameters of the carbonate system at both sides of the optimum.
Project(s):
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1Experimental treatment *Exp trtmBach, Lennart Thomas *
2Salinity *SalBach, Lennart Thomas *
3Temperature, water *Temp°CBach, Lennart Thomas *
4Radiation, photosynthetically active *PARµE/m2/sBach, Lennart Thomas *
5Light:Dark cycle *L:Dhh:hhBach, Lennart Thomas *Measured *
6Fugacity of carbon dioxide in seawater, standard deviation *fCO2 std dev±Bach, Lennart Thomas *Calculated using CO2SYS *
7Fugacity of carbon dioxide in seawater, standard deviation *fCO2 std dev±Bach, Lennart Thomas *Calculated using CO2SYS *
8Carbon, inorganic, dissolved *DICµmol/kgBach, Lennart Thomas *Photometry *
9Carbon, inorganic, dissolved *DICµmol/kgBach, Lennart Thomas *Photometry *
10Alkalinity, total *ATµmol/kgBach, Lennart Thomas *Potentiometric open-cell titration *
11Alkalinity, total *ATµmol/kgBach, Lennart Thomas *Potentiometric open-cell titration *
12pH *pHBach, Lennart Thomas *Titration potentiometric *T0; Free scale
13pH *pHBach, Lennart Thomas *Titration potentiometric *Tfin; Free scale
14Growth rate *µ#/dayBach, Lennart Thomas *Calculated *
15Emiliania huxleyi, diameter *E. huxleyi diamµmBach, Lennart Thomas *Scanning electron microscope (SEM) *
16Production of particulate organic carbon per cell *POC prodpg/#/dayBach, Lennart Thomas *Calculated *
17Particulate inorganic carbon production per cell *PIC prodpg/#/dayBach, Lennart Thomas *Calculated *
18Chlorophyll a, production, per cell *Chl a prodpg/#/dayBach, Lennart Thomas *Pigments, Turner fluorometer *
19Particulate inorganic carbon/particulate organic carbon ratio *PIC/POC ratioBach, Lennart Thomas *
20Carbonate system computation flag *CSC flagNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
21Carbon dioxide *CO2µmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *T0
22Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *T0
23Fugacity of carbon dioxide (water) at sea surface temperature (wet air) *fCO2water_SST_wetµatmNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *T0
24Bicarbonate ion *[HCO3]-µmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *T0
25Carbonate ion *[CO3]2-µmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *T0
26Alkalinity, total *ATµmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *T0
27Aragonite saturation state *Omega ArgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *T0
28Calcite saturation state *Omega CalNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *T0
29pH *pHNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *T0; Total scale
30Carbon dioxide *CO2µmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *Tfin
31Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *Tfin
32Fugacity of carbon dioxide (water) at sea surface temperature (wet air) *fCO2water_SST_wetµatmNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *Tfin
33Bicarbonate ion *[HCO3]-µmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *Tfin
34Carbonate ion *[CO3]2-µmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *Tfin
35Alkalinity, total *ATµmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *Tfin
36Aragonite saturation state *Omega ArgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *Tfin
37Calcite saturation state *Omega CalNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *Tfin
38pH *pHNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *Tfin; Total scale
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