Not logged in
PANGAEA.
Data Publisher for Earth & Environmental Science

de Kluijver, Anna; Soetaert, Karline; Schulz, Kai Georg; Riebesell, Ulf; Bellerby, Richard GJ; Middelburg, Jack J (2010): Phytoplankton-bacteria coupling under elevated CO2 levels: a stable isotope labelling study, 2010. doi:10.1594/PANGAEA.758223,
Supplement to: de Kluijver, A et al. (2010): Phytoplankton-bacteria coupling under elevated CO2 levels: a stable isotope labelling study. Biogeosciences, 7(11), 3783-3797, doi:10.5194/bg-7-3783-2010

Always quote above citation when using data! You can download the citation in several formats below.

RIS CitationBibTeX Citation

Abstract:
The potential impact of rising carbon dioxide (CO2) on carbon transfer from phytoplankton to bacteria was investigated during the 2005 PeECE III mesocosm study in Bergen, Norway. Sets of mesocosms, in which a phytoplankton bloom was induced by nutrient addition, were incubated under 1x (~350 µatm), 2x (~700 µatm), and 3x present day CO2 (~1050 µatm) initial seawater and sustained atmospheric CO2 levels for 3 weeks. 13C labelled bicarbonate was added to all mesocosms to follow the transfer of carbon from dissolved inorganic carbon (DIC) into phytoplankton and subsequently heterotrophic bacteria, and settling particles. Isotope ratios of polar-lipid-derived fatty acids (PLFA) were used to infer the biomass and production of phytoplankton and bacteria. Phytoplankton PLFA were enriched within one day after label addition, whilst it took another 3 days before bacteria showed substantial enrichment. Group-specific primary production measurements revealed that coccolithophores showed higher primary production than green algae and diatoms. Elevated CO2 had a significant positive effect on post-bloom biomass of green algae, diatoms, and bacteria. A simple model based on measured isotope ratios of phytoplankton and bacteria revealed that CO2 had no significant effect on the carbon transfer efficiency from phytoplankton to bacteria during the bloom. There was no indication of CO2 effects on enhanced settling based on isotope mixing models during the phytoplankton bloom, but this could not be determined in the post-bloom phase. Our results suggest that CO2effects are most pronounced in the post-bloom phase, under nutrient limitation.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1Experimental treatmentExp trtmde Kluijver, Anna
2Experiment dayExp daydayde Kluijver, Anna
3Phytoplankton, biomass as carbonPhytopl Cµg/lde Kluijver, AnnaTotal
4Phytoplankton, biomass as carbonPhytopl Cµg/lde Kluijver, Anna13C labelled
5Bacteria, biomass as carbonBact Cµg/lde Kluijver, AnnaTotal
6Bacteria, biomass as carbonBact Cµg/lde Kluijver, Anna13C labelled
7Coccolithophoridae, biomass as carbonCoccolith Cµg/lde Kluijver, AnnaTotal
8Coccolithophoridae, biomass as carbonCoccolith Cµg/lde Kluijver, Anna13C labelled
9Diatoms, biomass as carbonDiatom Cµg/lde Kluijver, AnnaTotal
10Diatoms, biomass as carbonDiatom Cµg/lde Kluijver, Anna13C labelled
11Green algae, biomass as carbonGreen algae Cµg/lde Kluijver, AnnaTotal
12Green algae, biomass as carbonGreen algae Cµg/lde Kluijver, Anna13 labelled
13Growth rateµ#/dayde Kluijver, AnnaCalculated, see reference(s)Coccolithophoridae
14Growth rateµ#/dayde Kluijver, AnnaCalculated, see reference(s)Diatoms
15Growth rateµ#/dayde Kluijver, AnnaCalculated, see reference(s)Green algae
16Growth rateµ#/dayde Kluijver, AnnaCalculated, see reference(s)Total phytoplankton
17Carbon, inorganic, dissolvedDICµmol/lde Kluijver, Anna
18Labelled phytoplankton in sediment traps relative to labelled phytoplankoton inPhytopl labelledde Kluijver, Anna
19Labelled bacteria in sediment traps relative to labelled bacteria in water columBact labelledde Kluijver, Anna
20Phytoplankton, delta-delta, weighedPhytopl delta-deltaper milde Kluijver, Annamodelled
21Bacteria, delta-delta, weighedBact delta-deltaper milde Kluijver, Annamodelled
Size:
3090 data points

Download Data

Download dataset as tab-delimited text (use the following character encoding: )

View dataset as HTML (shows only first 2000 rows)