Data Description

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Citation:
de Kluijver, A et al. (2010): Phytoplankton-bacteria coupling under elevated CO2 levels: a stable isotope labelling study, 2010. doi:10.1594/PANGAEA.758223,
Supplement to: de Kluijver, Anna; Soetaert, Karline; Schulz, Kai Georg; Riebesell, Ulf; Bellerby, Richard GJ; Middelburg, Jack J (2010): Phytoplankton-bacteria coupling under elevated CO2 levels: a stable isotope labelling study. Biogeosciences, 7(11), 3783-3797, doi:10.5194/bg-7-3783-2010
Abstract:
The potential impact of rising carbon dioxide (CO2) on carbon transfer from phytoplankton to bacteria was investigated during the 2005 PeECE III mesocosm study in Bergen, Norway. Sets of mesocosms, in which a phytoplankton bloom was induced by nutrient addition, were incubated under 1x (~350 µatm), 2x (~700 µatm), and 3x present day CO2 (~1050 µatm) initial seawater and sustained atmospheric CO2 levels for 3 weeks. 13C labelled bicarbonate was added to all mesocosms to follow the transfer of carbon from dissolved inorganic carbon (DIC) into phytoplankton and subsequently heterotrophic bacteria, and settling particles. Isotope ratios of polar-lipid-derived fatty acids (PLFA) were used to infer the biomass and production of phytoplankton and bacteria. Phytoplankton PLFA were enriched within one day after label addition, whilst it took another 3 days before bacteria showed substantial enrichment. Group-specific primary production measurements revealed that coccolithophores showed higher primary production than green algae and diatoms. Elevated CO2 had a significant positive effect on post-bloom biomass of green algae, diatoms, and bacteria. A simple model based on measured isotope ratios of phytoplankton and bacteria revealed that CO2 had no significant effect on the carbon transfer efficiency from phytoplankton to bacteria during the bloom. There was no indication of CO2 effects on enhanced settling based on isotope mixing models during the phytoplankton bloom, but this could not be determined in the post-bloom phase. Our results suggest that CO2effects are most pronounced in the post-bloom phase, under nutrient limitation.
Project(s):
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1Experimental treatment *Exp trtmde Kluijver, Anna *
2Experiment day *Exp daydayde Kluijver, Anna *
3Phytoplankton, biomass as carbon *Phytopl Cµg/lde Kluijver, Anna *Total
4Phytoplankton, biomass as carbon *Phytopl Cµg/lde Kluijver, Anna *13C labelled
5Bacteria, biomass as carbon *Bact Cµg/lde Kluijver, Anna *Total
6Bacteria, biomass as carbon *Bact Cµg/lde Kluijver, Anna *13C labelled
7Coccolithophoridae, biomass as carbon *Coccolith Cµg/lde Kluijver, Anna *Total
8Coccolithophoridae, biomass as carbon *Coccolith Cµg/lde Kluijver, Anna *13C labelled
9Diatoms, biomass as carbon *Diatom Cµg/lde Kluijver, Anna *Total
10Diatoms, biomass as carbon *Diatom Cµg/lde Kluijver, Anna *13C labelled
11Green algae, biomass as carbon *Green algae Cµg/lde Kluijver, Anna *Total
12Green algae, biomass as carbon *Green algae Cµg/lde Kluijver, Anna *13 labelled
13Growth rate *µ#/dayde Kluijver, Anna *Calculated, see reference(s) *Coccolithophoridae
14Growth rate *µ#/dayde Kluijver, Anna *Calculated, see reference(s) *Diatoms
15Growth rate *µ#/dayde Kluijver, Anna *Calculated, see reference(s) *Green algae
16Growth rate *µ#/dayde Kluijver, Anna *Calculated, see reference(s) *Total phytoplankton
17Carbon, inorganic, dissolved *DICµmol/lde Kluijver, Anna *
18Labelled phytoplankton in sediment traps relative to labelled phytoplankoton in *Phytopl labelledde Kluijver, Anna *
19Labelled bacteria in sediment traps relative to labelled bacteria in water colum *Bact labelledde Kluijver, Anna *
20Phytoplankton, delta-delta, weighed *Phytopl delta-deltaper milde Kluijver, Anna *modelled *
21Bacteria, delta-delta, weighed *Bact delta-deltaper milde Kluijver, Anna *modelled *
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