Citation:

bg-7-3783-2010

Name: Phytoplankton-bacteria coupling under elevated CO2 levels: a stable isotope labelling study, 2010
Published: 2010
License: https://creativecommons.org/licenses/by/3.0/

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Abstract:

The potential impact of rising carbon dioxide (CO2) on carbon transfer from phytoplankton to bacteria was investigated during the 2005 PeECE III mesocosm study in Bergen, Norway. Sets of mesocosms, in which a phytoplankton bloom was induced by nutrient addition, were incubated under 1x (~350 µatm), 2x (~700 µatm), and 3x present day CO2 (~1050 µatm) initial seawater and sustained atmospheric CO2 levels for 3 weeks. 13C labelled bicarbonate was added to all mesocosms to follow the transfer of carbon from dissolved inorganic carbon (DIC) into phytoplankton and subsequently heterotrophic bacteria, and settling particles. Isotope ratios of polar-lipid-derived fatty acids (PLFA) were used to infer the biomass and production of phytoplankton and bacteria. Phytoplankton PLFA were enriched within one day after label addition, whilst it took another 3 days before bacteria showed substantial enrichment. Group-specific primary production measurements revealed that coccolithophores showed higher primary production than green algae and diatoms. Elevated CO2 had a significant positive effect on post-bloom biomass of green algae, diatoms, and bacteria. A simple model based on measured isotope ratios of phytoplankton and bacteria revealed that CO2 had no significant effect on the carbon transfer efficiency from phytoplankton to bacteria during the bloom. There was no indication of CO2 effects on enhanced settling based on isotope mixing models during the phytoplankton bloom, but this could not be determined in the post-bloom phase. Our results suggest that CO2effects are most pronounced in the post-bloom phase, under nutrient limitation.

Project(s):

European Project on Ocean Acidification (EPOCA)

Funding:

Seventh Framework Programme (FP7), grant/award no. 211384 : European Project on Ocean Acidification

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Experimental treatment	Exp treat		de Kluijver, Anna
2	Experiment day	Exp day	day	de Kluijver, Anna
3	Phytoplankton, biomass as carbon	Phytopl C	µg/l	de Kluijver, Anna		Total
4	Phytoplankton, biomass as carbon	Phytopl C	µg/l	de Kluijver, Anna		13C labelled
5	Bacteria, biomass as carbon	Bact C	µg/l	de Kluijver, Anna		Total
6	Bacteria, biomass as carbon	Bact C	µg/l	de Kluijver, Anna		13C labelled
7	Coccolithophoridae, biomass as carbon	Coccolith C	µg/l	de Kluijver, Anna		Total
8	Coccolithophoridae, biomass as carbon	Coccolith C	µg/l	de Kluijver, Anna		13C labelled
9	Diatoms, biomass as carbon	Diatom C	µg/l	de Kluijver, Anna		Total
10	Diatoms, biomass as carbon	Diatom C	µg/l	de Kluijver, Anna		13C labelled
11	Green algae, biomass as carbon	Green algae C	µg/l	de Kluijver, Anna		Total
12	Green algae, biomass as carbon	Green algae C	µg/l	de Kluijver, Anna		13 labelled
13	Growth rate	µ	#/day	de Kluijver, Anna	Calculated, see reference(s)	Coccolithophoridae
14	Growth rate	µ	#/day	de Kluijver, Anna	Calculated, see reference(s)	Diatoms
15	Growth rate	µ	#/day	de Kluijver, Anna	Calculated, see reference(s)	Green algae
16	Growth rate	µ	#/day	de Kluijver, Anna	Calculated, see reference(s)	Total phytoplankton
17	Carbon, inorganic, dissolved	DIC	µmol/l	de Kluijver, Anna
18	Labelled phytoplankton in sediment traps relative to labelled phytoplankoton in water column	Phytopl labelled		de Kluijver, Anna
19	Labelled bacteria in sediment traps relative to labelled bacteria in water column	Bact labelled		de Kluijver, Anna
20	Phytoplankton, delta-delta, weighted	Phytopl delta-delta	‰	de Kluijver, Anna	modelled
21	Bacteria, delta-delta, weighted	Bact delta-delta	‰	de Kluijver, Anna	modelled

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Size:

3090 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Exp treat	2 Exp day [day]	3 Phytopl C [µg/l] (Total)	4 Phytopl C [µg/l] (13C labelled)	5 Bact C [µg/l] (Total)	6 Bact C [µg/l] (13C labelled)	7 Coccolith C [µg/l] (Total)	8 Coccolith C [µg/l] (13C labelled)	9 Diatom C [µg/l] (Total)	10 Diatom C [µg/l] (13C labelled)	11 Green algae C [µg/l] (Total)	12 Green algae C [µg/l] (13 labelled)	13 µ [#/day] (Coccolithophoridae, Calculate...)	14 µ [#/day] (Diatoms, Calculated, see refe...)	15 µ [#/day] (Green algae, Calculated, see ...)	16 µ [#/day] (Total phytoplankton, Calculat...)	17 DIC [µmol/l]	18 Phytopl labelled	19 Bact labelled	20 Phytopl delta-delta [‰] (modelled)	21 Bact delta-delta [‰] (modelled)
Bag_1	0	35.65	0.000	32.242	0.000	4.876	0.000	20.99	0.00	6.215	0.000					0.000			0.00	0.00
Bag_1	1	72.93	0.006	78.199	0.000	9.539	0.001	43.78	0.00	12.305	0.001					2.292			7.65	0.00
Bag_1	2	61.00	0.017	55.730	0.001	6.863	0.003	39.62	0.01	8.430	0.004					2.305			25.91	1.20
Bag_1	3	121.86	0.048	77.646	0.000	12.889	0.008	81.81	0.02	12.547	0.007					2.300			36.50	0.00
Bag_1	4	150.82	0.073	34.226	0.004	16.714	0.013	129.47	0.05	9.066	0.006					2.284	0.250	2.169	44.98	10.75
Bag_1	5	101.19	0.066	16.586	0.002	18.279	0.015	81.66	0.04	5.004	0.004					2.225			60.12	10.21
Bag_1	6	271.51	0.201	34.131	0.006	50.889	0.048	224.04	0.15	15.835	0.015					2.147			68.27	15.79
Bag_1	7	199.52	0.167	125.632	0.050	34.935	0.033	144.75	0.11	10.784	0.010					2.088	0.278	0.194	77.53	36.61
Bag_1	8	616.13	0.561	240.973	0.119	141.512	0.145	462.52	0.39	38.056	0.036					2.019			84.10	45.59
Bag_1	9	806.80	0.762	44.471	0.029	227.090	0.237	605.66	0.50	56.709	0.057					1.966			87.29	59.76
Bag_1	10	116.74	0.110	36.039	0.025	27.313	0.028	83.29	0.07	8.613	0.009					1.911	0.319	0.296	87.35	63.96
Bag_1	11	90.58	0.090	89.355	0.070	18.792	0.019	49.62	0.04	6.391	0.006					1.830			91.60	72.58
Bag_1	12	210.05	0.223	154.808	0.111	58.880	0.068	94.43	0.09	19.033	0.022					1.823			98.13	66.09
Bag_1	13	305.67	0.290	92.133	0.070	76.137	0.076	138.43	0.12	32.432	0.030					1.764	0.521	0.563	87.66	70.46
Bag_1	14	187.11	0.176	156.697	0.119	40.767	0.041	90.89	0.08	18.404	0.017					1.760			87.12	69.95
Bag_1	15	195.87	0.181	156.697	0.119	35.804	0.034	96.35	0.08	26.131	0.023					1.764			85.34	69.95
Bag_1	16	158.80	0.145	134.735	0.103	24.480	0.023	82.98	0.07	18.794	0.017					1.773	0.864	0.753	84.41	70.77
Bag_1	17	127.03	0.112	102.936	0.079	18.171	0.017	63.59	0.05	12.649	0.010					1.772			81.58	70.86
Bag_1	18	217.85	0.190	198.575	0.152	36.504	0.032	89.45	0.07	21.263	0.018					1.770			80.55	70.58
Bag_1	19																0.850	0.689
Bag_1	20	149.38	0.126	116.911	0.088	23.830	0.020	71.84	0.05	14.659	0.012					1.728			78.21	69.49
Bag_1	22	51.88	0.042	76.860	0.056	15.575	0.013	47.92	0.03	7.457	0.006					1.656			74.00	67.42
Bag_1	24	115.34	0.092	121.970	0.083	17.472	0.013	57.16	0.04	8.788	0.006					1.637			73.96	62.93
Bag_1	25											0.68	0.62	0.69	0.61	1.629
Bag_2	0	16.82	0.000	20.190	0.000	2.650	0.000	12.09	0.00	3.188	0.000					0.000			0.00	0.00
Bag_2	1															2.292
Bag_2	2	56.93	0.018	57.606	0.001	6.941	0.002	31.38	0.00	14.648	0.006					2.303			27.43	0.79
Bag_2	3	129.27	0.048	54.962	0.001	15.345	0.005	89.57	0.02	14.208	0.007					2.282			32.73	1.81
Bag_2	4	153.19	0.073	41.290	0.004	18.987	0.009	129.73	0.05	14.381	0.008					2.276	0.191	0.774	42.45	7.71
Bag_2	5	101.78	0.059	15.492	0.003	10.774	0.006	101.38	0.05	7.051	0.004					2.254			51.31	15.35
Bag_2	6	309.26	0.222	36.991	0.008	53.167	0.040	272.57	0.18	16.571	0.015					2.183			63.58	19.31
Bag_2	7	432.39	0.362	79.010	0.029	80.288	0.064	363.79	0.29	25.386	0.024					2.147	0.301	0.279	74.32	32.32
Bag_2	8	701.86	0.602	145.719	0.058	169.995	0.140	544.92	0.44	44.051	0.042					2.083			76.09	35.19
Bag_2	9	681.67	0.634	215.690	0.115	175.204	0.154	492.83	0.41	51.253	0.051					2.043			82.43	47.15
Bag_2	10	136.29	0.125	55.405	0.036	33.994	0.029	93.17	0.08	9.604	0.009					2.005	0.407	0.554	81.21	57.82
Bag_2	11	75.05	0.070	33.869	0.023	18.297	0.016	48.96	0.04	5.855	0.006					1.876			82.67	59.49
Bag_2	12	114.87	0.108	72.360	0.053	25.437	0.022	60.70	0.05	11.890	0.012					1.862			83.70	65.28
Bag_2	13	236.54	0.223	137.187	0.101	59.251	0.049	112.41	0.10	25.968	0.025					1.776	0.568	0.666	83.66	65.09
Bag_2	14	218.31	0.205	110.880	0.085	47.507	0.040	120.85	0.11	21.036	0.020					1.799			83.13	67.74
Bag_2	15	237.77	0.217	183.416	0.140	56.855	0.045	107.94	0.09	36.328	0.034					1.807			81.05	67.89
Bag_2	16	220.54	0.200	163.215	0.126	37.670	0.029	110.46	0.09	27.283	0.024					1.801	0.836	0.758	80.54	68.29
Bag_2	17	143.56	0.126	112.572	0.089	26.902	0.020	70.98	0.06	18.444	0.015					1.810			77.94	69.99
Bag_2	18	286.71	0.246	233.199	0.180	56.883	0.040	134.34	0.10	33.005	0.027					1.805			76.10	68.36
Bag_2	19																0.909	0.771
Bag_2	20	116.74	0.094	136.893	0.105	37.238	0.026	107.11	0.07	20.700	0.017					1.752			71.13	67.98
Bag_2	22	134.12	0.109	107.872	0.079	28.710	0.019	64.72	0.04	10.851	0.008					1.646			72.20	64.89
Bag_2	24	193.29	0.156	166.071	0.115	39.549	0.027	83.14	0.06	12.209	0.009					1.630			71.53	61.36
Bag_2	25											0.81	0.54	0.65	0.59	1.623
Bag_3	0	21.62	0.000	42.349	0.000	2.947	0.000	11.35	0.00	3.136	0.000					0.000			0.00	0.00
Bag_3	1															2.292
Bag_3	2	84.03	0.025	77.361	0.000	11.057	0.004	48.29	0.00	13.089	0.005					2.281			26.81	0.00
Bag_3	3	107.28	0.044	50.511	0.001	18.640	0.008	66.94	0.01	13.142	0.008					2.284			36.39	1.73
Bag_3	4	138.13	0.067	40.122	0.005	17.574	0.011	103.79	0.03	12.347	0.008					2.266	0.297	2.038	43.12	10.28
Bag_3	5	114.49	0.064	20.813	0.004	16.529	0.012	97.45	0.04	6.653	0.005					2.187			49.60	15.33
Bag_3	6	194.69	0.130	25.523	0.003	31.420	0.025	157.68	0.09	11.276	0.010					2.110			59.60	9.28
Bag_3	7	273.21	0.214	38.510	0.012	48.417	0.039	211.24	0.14	19.278	0.019					2.074	0.339	0.519	69.92	28.19
Bag_3	8	424.78	0.356	95.402	0.036	96.234	0.084	318.08	0.23	31.578	0.030					1.988			74.96	34.11
Bag_3	9	656.32	0.585	152.295	0.068	160.918	0.148	485.61	0.37	51.144	0.047					1.954			79.60	40.02
Bag_3	10	131.08	0.116	42.974	0.025	27.864	0.025	107.63	0.08	15.360	0.012					1.892	0.520	0.626	79.29	51.79
Bag_3	11	166.78	0.148	85.292	0.052	35.940	0.032	122.87	0.09	13.339	0.012					1.702			79.19	54.84
Bag_3	12	173.00	0.151	109.029	0.070	39.177	0.034	113.40	0.09	15.554	0.014					1.680			78.17	57.52
Bag_3	13	208.74	0.181	121.741	0.086	45.302	0.038	121.60	0.09	18.589	0.015					1.650	0.784	0.737	77.50	63.40
Bag_3	14	111.24	0.097	71.553	0.051	23.021	0.019	63.17	0.05	11.550	0.009					1.626			77.71	63.67
Bag_3	15	203.59	0.175	159.413	0.113	39.021	0.031	105.26	0.08	27.318	0.022					1.631			76.81	63.60
Bag_3	16	155.62	0.129	129.281	0.097	22.052	0.016	85.87	0.06	14.591	0.011					1.637	0.948	0.796	74.06	66.94
Bag_3	17	143.75	0.120	113.593	0.083	19.334	0.015	83.62	0.06	11.674	0.009					1.624			74.66	65.68
Bag_3	18	205.09	0.167	179.940	0.132	32.077	0.022	109.16	0.08	18.482	0.013					1.637			72.78	65.68
Bag_3	19									0.000							0.971	0.918
Bag_3	20	255.92	0.209	198.420	0.141	50.721	0.039	129.93	0.09	33.213	0.024					1.605			72.91	63.34
Bag_3	22	97.54	0.077	119.469	0.085	17.142	0.012	54.01	0.04	8.001	0.005					1.537			70.37	63.77
Bag_3	24	108.87	0.086	109.115	0.073	15.391	0.010	58.82	0.04	7.330	0.005					1.522			70.18	59.83
Bag_3	25									0.000		0.63	0.54	0.56	0.55	1.512
Bag_4	0	26.91	0.000	41.978	0.000	4.551	0.000	23.81	0.00	4.993	0.000					0.000			0.00	0.00
Bag_4	1									0.000						2.292
Bag_4	2	78.27	0.024	59.470	0.001	10.686	0.005	44.37	0.00	12.817	0.006					2.270			29.81	1.37
Bag_4	3	95.31	0.039	41.277	0.001	11.565	0.007	64.66	0.02	9.643	0.005					2.262			39.24	2.41
Bag_4	4	133.01	0.067	24.229	0.002	12.210	0.009	108.27	0.04	9.431	0.006					2.241	0.318	1.666	48.11	7.43
Bag_4	5	125.81	0.074	24.592	0.003	18.076	0.014	112.22	0.05	8.235	0.006					2.166			56.09	13.53
Bag_4	6	252.57	0.187	42.061	0.008	46.158	0.043	199.80	0.13	16.132	0.014					2.063			70.96	19.19
Bag_4	7	265.22	0.227	46.752	0.018	62.156	0.062	201.41	0.14	17.903	0.016					2.045	0.357	0.366	82.09	37.83
Bag_4	8	577.89	0.532	87.157	0.039	155.193	0.160	417.92	0.34	44.472	0.042					1.943			88.40	43.35
Bag_4	9	740.91	0.702	159.568	0.078	190.650	0.200	529.40	0.45	54.080	0.051					1.941			90.99	47.03
Bag_4	10	132.69	0.127	41.433	0.026	32.599	0.034	92.73	0.08	13.775	0.012					1.888	0.445	0.511	91.58	59.94
Bag_4	11	121.80	0.114	60.489	0.040	43.766	0.044	70.24	0.06	11.145	0.010					1.793			89.46	64.08
Bag_4	12	131.35	0.125	80.074	0.061	32.478	0.033	80.66	0.07	13.164	0.012					1.790			91.10	73.31
Bag_4	13	194.23	0.183	119.016	0.097	38.548	0.039	109.47	0.09	16.805	0.015					1.779	0.623	0.652	90.65	78.62
Bag_4	14	234.18	0.217	115.996	0.093	38.916	0.038	132.90	0.11	18.707	0.016					1.737			88.93	76.67
Bag_4	15	259.44	0.237	197.089	0.138	50.096	0.048	134.03	0.11	29.781	0.025					1.741			87.57	67.37
Bag_4	16	68.56	0.060	74.275	0.060	12.390	0.012	36.48	0.03	7.206	0.005					1.752	0.873	0.816	84.55	77.61
Bag_4	17	193.05	0.172	153.210	0.121	23.571	0.022	104.02	0.08	17.646	0.014					1.734			85.48	75.81
Bag_4	18	213.44	0.185	205.339	0.162	34.753	0.031	113.43	0.08	20.254	0.014					1.753			83.37	75.76
Bag_4	19																0.879	0.772
Bag_4	20	131.55	0.111	95.247	0.073	28.172	0.024	79.04	0.05	13.426	0.011					1.714			81.23	73.55
Bag_4	22	135.70	0.114	125.993	0.098	38.233	0.033	63.04	0.04	11.230	0.008					1.689			80.40	74.30
Bag_4	24	108.97	0.089	103.141	0.074	23.780	0.020	51.60	0.03	6.429	0.004					1.663			78.20	69.09
Bag_4	25											0.66	0.54	0.54	0.56	1.651
Bag_5	0	18.23	0.000	34.806	0.000	2.643	0.000	15.79	0.00	4.675	0.000					0.000			0.00	0.00
Bag_5	1	70.05	0.007	66.210	0.000	10.069	0.001	38.69	0.00	12.737	0.002					2.292			9.58	0.00
Bag_5	2	62.78	0.018	44.958	0.001	7.288	0.003	40.06	0.00	7.862	0.004					2.275			27.28	2.45
Bag_5	3	89.00	0.037	41.015	0.001	9.428	0.005	63.11	0.02	16.647	0.007					2.260			39.66	1.35
Bag_5	4	89.29	0.045	20.308	0.001	8.919	0.006	75.73	0.03	9.383	0.005					2.220	0.300	2.149	48.02	5.91
Bag_5	5	72.97	0.041	27.903	0.003	15.921	0.011	68.92	0.03	24.653	0.006					2.158			54.13	9.51
Bag_5	6	296.02	0.220	48.645	0.009	61.237	0.054	228.55	0.15	18.636	0.017					2.051			71.38	18.59
Bag_5	7	343.34	0.285	68.440	0.017	77.920	0.072	275.56	0.20	21.321	0.019					2.014	0.304	0.522	79.77	23.65
Bag_5	8	603.30	0.536	117.754	0.051	151.078	0.145	446.45	0.35	42.407	0.041					1.921			85.21	41.84
Bag_5	9	760.26	0.697	194.599	0.091	194.016	0.187	554.90	0.46	50.425	0.048					1.931			88.00	45.08
Bag_5	10	144.87	0.136	55.923	0.035	37.180	0.036	99.33	0.09	9.818	0.009					1.895	0.414	0.510	90.07	59.63
Bag_5	11	91.96	0.084	67.062	0.044	29.326	0.028	92.43	0.08	9.276	0.009					1.789			87.86	62.86
Bag_5	12	117.44	0.108	73.843	0.051	27.322	0.025	65.68	0.05	9.864	0.009					1.789			88.32	66.12
Bag_5	13	201.60	0.191	122.965	0.092	49.525	0.047	95.24	0.08	20.291	0.019					1.773	0.673	0.645	90.85	71.79
Bag_5	14	119.37	0.111	62.269	0.049	24.301	0.023	67.76	0.06	10.738	0.010					1.739			89.45	74.83
Bag_5	15	199.51	0.181	162.848	0.123	36.961	0.033	101.33	0.08	24.918	0.022					1.734			86.85	72.63
Bag_5	16	129.45	0.116	90.370	0.071	19.946	0.018	71.89	0.06	12.741	0.011					1.751	0.893	0.815	85.96	75.69
Bag_5	17	176.04	0.152	121.102	0.092	27.318	0.023	84.93	0.06	18.581	0.015					1.733			82.89	72.75
Bag_5	18	180.79	0.155	154.250	0.120	29.229	0.023	95.58	0.06	17.651	0.014					1.744			82.44	74.44
Bag_5	19																0.859	0.784
Bag_5	20	250.92	0.214	191.258	0.147	52.558	0.043	114.54	0.08	23.831	0.020					1.704			81.67	73.88
Bag_5	22	126.24	0.105	91.773	0.068	29.474	0.023	58.46	0.04	7.705	0.006					1.685			79.97	71.43
Bag_5	24	148.08	0.120	123.799	0.088	31.575	0.024	67.14	0.05	8.984	0.007					1.650			77.93	67.93
Bag_5	25											0.71	0.64	0.51	0.71	1.635
Bag_6	0	68.25	0.000	78.313	0.000	6.352	0.000	49.78	0.00	11.935	0.000					0.000			0.00	0.00
Bag_6	1															2.292
Bag_6	2	89.96	0.026	67.936	0.001	11.650	0.004	53.40	0.01	12.248	0.006					2.254			19.70	0.72
Bag_6	3	123.87	0.046	59.102	0.004	13.731	0.006	86.92	0.02	15.876	0.008					2.235			25.38	4.49
Bag_6	4	144.81	0.073	27.369	0.000	15.059	0.009	115.29	0.05	10.835	0.008					2.199	0.300	15.434	34.45	-4.18
Bag_6	5	68.59	0.042	19.536	0.002	9.617	0.006	61.17	0.03	4.827	0.004					2.132			41.61	5.65
Bag_6	6	326.14	0.236	45.642	0.007	64.586	0.055	255.49	0.16	20.802	0.019					1.996			49.49	10.61
Bag_6	7	467.23	0.398	73.027	0.028	115.329	0.103	340.82	0.26	37.066	0.032					1.918	0.391	0.462	58.36	25.86
Bag_6	8	515.12	0.462	94.764	0.060	127.813	0.116	393.14	0.31	42.816	0.039					1.889			61.34	43.00
Bag_6	9	616.21	0.574	141.576	0.064	156.743	0.148	468.95	0.38	44.344	0.042					1.836			63.77	31.09
Bag_6	10	124.53	0.118	46.544	0.029	30.624	0.029	91.58	0.08	8.946	0.008					1.788	0.497	0.533	64.72	42.58
Bag_6	11	49.57	0.046	98.773	0.064	14.869	0.014	38.82	0.03	4.842	0.005					1.649			64.04	44.25
Bag_6	12	199.24	0.182	151.003	0.102	39.538	0.036	126.72	0.10	16.851	0.015					1.655			62.62	46.01
Bag_6	13	172.63	0.158	108.164	0.082	37.352	0.033	95.40	0.08	16.813	0.014					1.639	0.791	0.909	62.64	52.11
Bag_6	14	126.09	0.115	62.019	0.051	24.768	0.022	71.72	0.06	11.852	0.010					1.608			62.64	55.97
Bag_6	15	171.45	0.152	141.412	0.103	31.573	0.027	96.36	0.08	20.347	0.017					1.621			60.77	49.78
Bag_6	16	106.21	0.093	78.002	0.060	16.587	0.014	61.27	0.05	8.816	0.007					1.628	1.003	0.867	59.81	52.50
Bag_6	17	156.16	0.136	120.080	0.089	20.736	0.016	78.65	0.06	12.522	0.009					1.629			59.65	50.62
Bag_6	18	166.31	0.141	163.613	0.124	23.671	0.018	92.50	0.06	14.239	0.011					1.632			58.15	51.86
Bag_6	19																0.979	0.849
Bag_6	20	208.21	0.177	139.065	0.103	45.337	0.037	98.35	0.07	21.175	0.017					1.586			58.08	50.93
Bag_6	22	117.34	0.097	82.727	0.061	26.134	0.020	55.37	0.04	6.404	0.004					1.569			56.48	50.78
Bag_6	24	163.48	0.136	119.699	0.082	34.738	0.027	73.00	0.05	8.099	0.006					1.563			57.09	46.61
Bag_6	25											0.79	0.62	0.61	0.66	1.568
Bag_7	0	48.68	0.000	54.635	0.000	7.349	0.000	34.17	0.00	9.455	0.000					0.000			0.00	0.00
Bag_7	1															2.292
Bag_7	2	88.51	0.029	68.008	0.000	13.000	0.005	53.69	0.01	13.148	0.005					2.261			31.11	0.50
Bag_7	3	105.27	0.048	45.400	0.003	11.107	0.006	70.22	0.02	10.954	0.006					2.243			44.25	7.30
Bag_7	4	99.57	0.062	17.759	0.003	8.941	0.007	82.79	0.04	7.008	0.005					2.226	0.265	0.754	59.96	16.68
Bag_7	5	90.35	0.060	13.850	0.002	11.566	0.009	76.98	0.05	5.033	0.004					2.146			64.24	12.46
Bag_7	6	357.20	0.291	47.394	0.005	68.795	0.065	263.48	0.20	22.255	0.019					1.993			78.28	10.84
Bag_7	7	545.57	0.496	95.067	0.036	132.813	0.131	378.73	0.31	36.467	0.034					2.012	0.305	0.488	87.39	36.50
Bag_7	8	562.58	0.549	133.116	0.062	150.937	0.153	395.91	0.36	39.474	0.038					1.999			93.83	44.62
Bag_7	9	832.12	0.836	208.735	0.109	214.025	0.221	592.52	0.55	56.758	0.053					1.937			96.54	50.00
Bag_7	10	217.81	0.219	81.865	0.056	58.556	0.061	139.98	0.13	18.036	0.017					1.822	0.448	0.553	96.78	65.90
Bag_7	11	62.61	0.060	16.426	0.012	8.272	0.008	27.46	0.02	5.972	0.005					1.722			91.80	71.46
Bag_7	12	19.09	0.018	49.919	0.039	8.123	0.008	13.74	0.01	3.663	0.003					1.727			90.32	75.23
Bag_7	13	151.97	0.150	83.412	0.069	35.245	0.035	66.02	0.06	14.483	0.013					1.711	0.719	0.574	94.75	78.99
Bag_7	14	68.47	0.065	34.641	0.029	14.557	0.014	36.72	0.03	5.936	0.005					1.681			91.64	81.41
Bag_7	15	192.78	0.185	133.758	0.108	37.561	0.035	95.42	0.08	23.684	0.020					1.688			92.08	77.51
Bag_7	16	89.10	0.083	70.777	0.059	13.080	0.012	48.98	0.04	8.382	0.006					1.698	0.913	0.774	89.82	79.62
Bag_7	17	133.89	0.121	72.784	0.060	18.831	0.016	73.82	0.06	8.745	0.006					1.699			86.81	78.68
Bag_7	18	202.65	0.184	173.223	0.142	30.036	0.026	105.60	0.08	14.359	0.011					1.705			87.24	78.85
Bag_7	19																0.876	0.829
Bag_7	20	220.79	0.195	177.012	0.141	49.113	0.040	122.36	0.08	14.745	0.011					1.639			84.86	76.49
Bag_7	22	64.47	0.055	63.095	0.049	13.606	0.011	36.13	0.03	5.200	0.004					1.610			82.65	74.46
Bag_7	24	97.05	0.084	99.294	0.074	18.948	0.015	46.60	0.03	3.354	0.002					1.616			83.17	71.97
Bag_7	25											0.80	0.62	0.67	0.66	1.616
Bag_8	0	21.03	0.000	27.196	0.000	3.084	0.000	15.94	0.00	3.482	0.000					0.000			0.00	0.00
Bag_8	1	64.00	0.008	66.094	0.000	8.692	0.001	35.76	0.00	9.828	0.002					2.292			11.75	0.41
Bag_8	2	55.82	0.016	40.054	0.000	7.405	0.002	43.02	0.01	8.719	0.003					2.225			27.67	0.00
Bag_8	3	121.10	0.053	48.076	0.004	15.160	0.008	84.06	0.03	12.404	0.007					2.205			42.34	8.20
Bag_8	4	94.41	0.054	19.399	0.002	9.552	0.006	80.89	0.04	6.358	0.005					2.179	0.327	0.934	55.56	9.29
Bag_8	5	71.25	0.046	12.124	0.001	10.684	0.006	63.56	0.04	4.963	0.003					2.087			61.91	6.24
Bag_8	6	310.26	0.250	45.337	0.008	66.688	0.058	231.15	0.18	20.622	0.018					1.959			77.71	16.52
Bag_8	7	453.47	0.407	94.686	0.034	108.904	0.099	347.73	0.29	33.680	0.028					1.977	0.320	0.484	86.47	34.48
Bag_8	8	614.40	0.588	151.858	0.067	168.644	0.161	426.95	0.38	44.601	0.043					1.945			92.28	42.41
Bag_8	9	519.94	0.502	151.333	0.074	138.853	0.132	340.33	0.31	39.552	0.036					1.897			93.04	47.28
Bag_8	10	198.95	0.196	84.723	0.056	55.258	0.054	115.02	0.11	15.904	0.015					1.861	0.460	0.590	94.93	63.23
Bag_8	11	170.97	0.167	87.436	0.061	52.443	0.050	89.84	0.08	15.620	0.015					1.751			93.93	67.25
Bag_8	12	61.55	0.059	40.080	0.031	13.900	0.013	34.74	0.03	8.419	0.008					1.741			91.74	73.40
Bag_8	13	161.09	0.156	103.755	0.083	42.716	0.039	75.41	0.07	16.457	0.015					1.743	0.665	0.595	93.56	77.15
Bag_8	14	69.18	0.066	38.320	0.031	14.031	0.013	38.07	0.03	6.178	0.005					1.698			91.44	77.83
Bag_8	15	176.98	0.167	145.738	0.116	35.242	0.031	80.87	0.07	22.895	0.020					1.715			90.98	76.74
Bag_8	16	109.36	0.101	84.936	0.070	17.695	0.015	58.08	0.05	10.042	0.008					1.719	0.859	0.759	88.92	79.04
Bag_8	17	89.20	0.082	67.047	0.053	14.949	0.013	50.11	0.04	7.355	0.006					1.725			88.13	75.47
Bag_8	18	170.25	0.154	147.244	0.118	29.566	0.024	86.24	0.07	12.986	0.011					1.733			87.12	77.34
Bag_8	19																0.831	0.844
Bag_8	20	163.66	0.142	139.706	0.109	41.362	0.032	76.92	0.06	12.116	0.009					1.676			83.78	75.34
Bag_8	22	126.41	0.112	117.923	0.089	30.786	0.026	65.42	0.04	6.550	0.005					1.666			85.07	72.59
Bag_8	24	95.68	0.082	92.908	0.066	25.690	0.021	45.40	0.03	3.342	0.003					1.664			82.70	68.05
Bag_8	25											0.76	0.53	0.60	0.60	1.663
Bag_9	0	37.47	0.000	49.432	0.000	6.951	0.000	26.63	0.00	7.097	0.000					0.000			0.00	0.00
Bag_9	1															2.292
Bag_9	2	56.56	0.017	50.409	0.000	7.842	0.003	40.61	0.01	8.688	0.004					2.237			29.57	0.00
Bag_9	3	119.99	0.052	48.660	0.004	13.848	0.007	79.31	0.03	12.502	0.007					2.213			43.26	7.43
Bag_9	4	52.23	0.029	9.595	0.000	5.490	0.004	44.51	0.02	3.036	0.002					2.189	0.237	1.839	55.85	2.67
Bag_9	5	52.34	0.033	9.325	0.001	5.545	0.004	49.11	0.03	2.780	0.002					2.074			62.08	9.44
Bag_9	6	288.41	0.225	44.574	0.017	54.251	0.049	230.30	0.17	17.482	0.016					1.960			77.78	37.00
Bag_9	7	293.18	0.261	46.354	0.016	73.129	0.070	220.85	0.18	18.314	0.016					1.951	0.356	0.555	88.97	35.12
Bag_9	8	802.94	0.747	176.217	0.080	251.115	0.249	488.21	0.41	57.955	0.054					1.925			92.92	45.14
Bag_9	9	769.05	0.736	183.345	0.101	252.245	0.243	457.83	0.39	63.682	0.060					1.862			95.58	54.74
Bag_9	10	227.43	0.219	61.254	0.040	115.888	0.110	122.92	0.10	18.339	0.017					1.838	0.396	0.449	96.20	65.95
Bag_9	11	167.88	0.137	90.495	0.056	38.717	0.032	92.96	0.07	14.757	0.011					1.806			81.26	61.52
Bag_9	12	59.71	0.058	30.318	0.023	17.352	0.017	28.28	0.02	7.404	0.007					1.800			96.61	77.25
Bag_9	13	97.08	0.094	54.123	0.045	26.028	0.025	44.53	0.04	9.101	0.008					1.787	0.655	0.600	97.07	83.90
Bag_9	14	113.34	0.109	53.255	0.044	27.040	0.026	54.38	0.05	11.449	0.010					1.729			95.75	82.93
Bag_9	15	136.85	0.132	93.251	0.077	26.228	0.025	66.83	0.06	14.236	0.013					1.756			96.24	82.41
Bag_9	16	69.25	0.064	47.246	0.040	15.826	0.015	34.58	0.03	5.555	0.004					1.757	0.874	0.734	92.79	84.60
Bag_9	17	52.17	0.047	35.049	0.029	6.877	0.006	32.77	0.02	3.747	0.003					1.763			90.48	83.11
Bag_9	18	128.94	0.116	141.769	0.118	25.206	0.022	80.17	0.06	11.876	0.010					1.770			89.55	82.99
Bag_9	19																0.859	0.677
Bag_9	20	156.44	0.136	97.942	0.080	41.826	0.035	80.00	0.06	11.208	0.009					1.682			86.80	81.21
Bag_9	22	164.75	0.141	90.217	0.072	44.002	0.037	66.15	0.05	7.538	0.006					1.676			85.20	80.10
Bag_9	24	126.55	0.107	81.750	0.061	29.944	0.025	54.82	0.04	4.225	0.003					1.648			84.52	74.18
Bag_9	25											1.01	0.67	0.88	0.78	1.638