Mertens, Kenneth Neil; Verhoeven, Koen; Verleye, Thomas; Louwye, Stephen; Amorim, Ana; Ribeiro, Sofia; Deaf, Amr S; Harding, Ian C; De Schepper, Stijn; González, Catalina; Kodrans-Nsiah, Monika; de Vernal, Anne; Henry, Maryse; Radi, Taoufik; Dybkjaer, Karen; Poulsen, Niels E; Feist-Burkhardt, Susanne; Chitolie, Jonah; Heilmann-Clausen, Claus; Londeix, Laurent; Turon, Jean-Louis; Marret, Fabienne; Matthiessen, Jens; McCarthy, Francine M G; Prasad, Vandana; Pospelova, Vera; Hughes, Jane E Kyffin; Riding, James B; Rochon, André; Sangiorgi, Francesca; Welters, Natasja; Sinclair, Natalie; Thun, Christian; Soliman, Ali; Van Nieuwenhove, Nicolas; Vink, Annemiek; Young, Martin (2009): Absolute abundances of dinoflagellate cysts in surface sediment samples from four sites: Lycopodium marker-grain method [dataset publication series]. PANGAEA, https://doi.org/10.1594/PANGAEA.754885, Supplement to: Mertens, KN et al. (2009): Determining the absolute abundance of dinoflagellate cysts in recent marine sediments: The Lycopodium marker-grain method put to the test. Review of Palaeobotany and Palynology, Review of Palaeobotany and Palynology, 157(3-4), 238-252, https://doi.org/10.1016/j.revpalbo.2009.05.004
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Abstract:
Absolute abundances (concentrations) of dinoflagellate cysts are often determined through the addition of Lycopodium clavatum marker-grains as a spike to a sample before palynological processing. An inter-laboratory calibration exercise was set up in order to test the comparability of results obtained in different laboratories, each using its own preparation method. Each of the 23 laboratories received the same amount of homogenized splits of four Quaternary sediment samples. The samples originate from different localities and consisted of a variety of lithologies. Dinoflagellate cysts were extracted and counted, and relative and absolute abundances were calculated. The relative abundances proved to be fairly reproducible, notwithstanding a need for taxonomic calibration. By contrast, excessive loss of Lycopodium spores during sample preparation resulted in non-reproducibility of absolute abundances. Use of oxidation, KOH, warm acids, acetolysis, mesh sizes larger than 15 µm and long ultrasonication (> 1 min) must be avoided to determine reproducible absolute abundances. The results of this work therefore indicate that the dinoflagellate cyst worker should make a choice between using the proposed standard method which circumvents critical steps, adding Lycopodium tablets at the end of the preparation and using an alternative method.
Project(s):
Coverage:
Median Latitude: 27.919075 * Median Longitude: -4.666717 * South-bound Latitude: -9.933700 * West-bound Longitude: -16.670000 * North-bound Latitude: 54.690000 * East-bound Longitude: 2.930000
Event(s):
Benguela * Latitude: -9.933700 * Longitude: 0.903130 * Comment: The Benguela sample consists of a mixture of sediment samples collected offshore Walvis Bay, at a water depth of about 200 m during Meteor cruise M63/2.
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Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
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4 datasets
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Datasets listed in this publication series
- Mertens, KN; Verhoeven, K; Verleye, T et al. (2009): Absolute abundances of dinoflagellate cysts insurface sediment samples from the Benguela site. https://doi.org/10.1594/PANGAEA.754819
- Mertens, KN; Verhoeven, K; Verleye, T et al. (2009): Absolute abundances of dinoflagellate cysts in surface sediment samples from the Celtic Sea. https://doi.org/10.1594/PANGAEA.754806
- Mertens, KN; Verhoeven, K; Verleye, T et al. (2009): Absolute abundances of dinoflagellate cysts in surface sediment samples from the North Sea. https://doi.org/10.1594/PANGAEA.754775
- Mertens, KN; Verhoeven, K; Verleye, T et al. (2009): Absolute abundances of dinoflagellate cysts in surface sediment samples from NW Africa. https://doi.org/10.1594/PANGAEA.754816