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Fiorini, Sarah; Middelburg, Jack J; Gattuso, Jean-Pierre (2010): Seawater carbonate chemistry and biological processes during experiments with haploid and diploid life stages of Emiliania huxleyi, Calcidiscus leptoporus and Syracosphaera pulchra [dataset]. Laboratoire d'Océanographie de Villefranche, PANGAEA, https://doi.org/10.1594/PANGAEA.733912, Supplement to: Fiorini, Sarah; Gattuso, Jean-Pierre; van Rijiswijk, Pieter; Middelburg, Jack J (2010): Coccolithophores lipid and carbon isotope composition and their variability related to changes in seawater carbonate chemistry. Journal of Experimental Marine Biology and Ecology, 394(1-2), 74-85, https://doi.org/10.1016/j.jembe.2010.07.020

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Abstract:
The response of Emiliania huxleyi (Lohmann), Calcidiscus leptoporus (Murray and Blackman), and Syracosphaera pulchra (Lohmann) to elevated partial pressure of carbon dioxide (pCO2) was investigated in batch cultures. For the first time, we reported on the response of the non-calcifying (haploid) life stage of these three species. Growth rate, cell size, particulate inorganic (PIC) and particulate organic carbon (POC) of both life stages were measured at two different pCO2 (400 and 760 ppm) and their organic and inorganic carbon production calculated. The two life stages within the same species generally exhibited a similar response to elevated pCO2, the response of the haploid stage being often more pronounced than that of the diploid stage. The growth rate was consistently higher at elevated pCO2 but the response of other processes varied among species. Calcification rate of C. leptoporus and of S. pulchra did not change at elevated pCO2 while it increased in E. huxleyi. Particulate organic carbon production and cell size of both life stages of S. pulchra and of the haploid stage of E. huxleyi markedly decreased at elevated pCO2. It remained unaltered in the diploid stage of E. huxleyi and C. leptoporus and increased in the haploid stage of the latter. The PIC:POC ratio increased in E. huxleyi and was constant in C. leptoporus and S. pulchra. Elevated pCO2 has a significant effect on these three coccolithophores species, the haploid stage being more sensitive. This must be taken into account when predicting the fate of coccolithophores in the future ocean.
Keyword(s):
Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcidiscus leptoporus; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Single species; Syracosphaera pulchra
Funding:
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1SpeciesSpeciesFiorini, Sarah
2Sample IDSample IDFiorini, Sarah
3Carbonate system computation flagCSC flagFiorini, Sarah
4Radiation, photosynthetically activePARµmol/m2/sFiorini, Sarah
5SalinitySalFiorini, Sarah
6Temperature, waterTemp°CFiorini, Sarah
7Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmFiorini, SarahMean
8pHpHFiorini, SarahMean, total scale
9Emiliania huxleyiE. huxleyi#/lFiorini, Sarah
10Calcidiscus leptoporusC. leptoporus#/lFiorini, Sarah
11Syracosphaera pulchraS. pulchra#/lFiorini, Sarah
12Growth rateµ#/dayFiorini, Sarah
13Carbon, inorganic, dissolvedDICµmol/kgFiorini, SarahCoulometric titrationMean
14Alkalinity, totalATµmol/kgFiorini, SarahTitration potentiometricMean
15Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Mean
16Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Mean
17Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Mean
18Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Mean
19Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Mean
20Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Mean
21Carbon, total, particulateTPC%Fiorini, SarahMass spectrometer Thermo Electron Flash EA 1122 Analyzer
22Carbon, total, particulateTPCµg/lFiorini, SarahMass spectrometer Thermo Electron Flash EA 1122 Analyzer
23Carbon, total, particulate, per cellTPC/cellpg/#Fiorini, SarahMass spectrometer Thermo Electron Flash EA 1122 Analyzer
24Carbon, organic, particulatePOC%Fiorini, SarahMass spectrometer Thermo Electron Flash EA 1122 Analyzer
25Carbon, organic, particulatePOCµg/lFiorini, SarahMass spectrometer Thermo Electron Flash EA 1122 Analyzer
26Carbon, organic, particulate, per cellPOC/cellpg/#Fiorini, SarahMass spectrometer Thermo Electron Flash EA 1122 Analyzer
27Carbon, inorganic, particulate, per cellPIC/cellpg/#Fiorini, SarahCalculated
28δ13C, particulate organic carbonδ13C POC‰ PDBFiorini, Sarah
29δ13C, dissolved inorganic carbonδ13C DIC‰ PDBFiorini, Sarah
30Nitrogen, totalTN%Fiorini, Sarah
31NitrogenNµg/lFiorini, Sarah
32Nitrogen per cellN/cellpg/#Fiorini, Sarah
33Carbon/Nitrogen ratioC/NFiorini, Sarah
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
2170 data points

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