Citation:

j.jembe.2010.07.020

Name: Seawater carbonate chemistry and biological processes during experiments with haploid and diploid life stages of Emiliania huxleyi, Calcidiscus leptoporus and Syracosphaera pulchra
Published: 2010-02-18
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcidiscus leptoporus; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Single species; Syracosphaera pulchra

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Abstract:

The response of Emiliania huxleyi (Lohmann), Calcidiscus leptoporus (Murray and Blackman), and Syracosphaera pulchra (Lohmann) to elevated partial pressure of carbon dioxide (pCO2) was investigated in batch cultures. For the first time, we reported on the response of the non-calcifying (haploid) life stage of these three species. Growth rate, cell size, particulate inorganic (PIC) and particulate organic carbon (POC) of both life stages were measured at two different pCO2 (400 and 760 ppm) and their organic and inorganic carbon production calculated. The two life stages within the same species generally exhibited a similar response to elevated pCO2, the response of the haploid stage being often more pronounced than that of the diploid stage. The growth rate was consistently higher at elevated pCO2 but the response of other processes varied among species. Calcification rate of C. leptoporus and of S. pulchra did not change at elevated pCO2 while it increased in E. huxleyi. Particulate organic carbon production and cell size of both life stages of S. pulchra and of the haploid stage of E. huxleyi markedly decreased at elevated pCO2. It remained unaltered in the diploid stage of E. huxleyi and C. leptoporus and increased in the haploid stage of the latter. The PIC:POC ratio increased in E. huxleyi and was constant in C. leptoporus and S. pulchra. Elevated pCO2 has a significant effect on these three coccolithophores species, the haploid stage being more sensitive. This must be taken into account when predicting the fate of coccolithophores in the future ocean.

Keyword(s):

Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcidiscus leptoporus; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Single species; Syracosphaera pulchra

Project(s):

European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)

European Project on Ocean Acidification (EPOCA)

Ocean Acidification International Coordination Centre (OA-ICC)

Funding:

Seventh Framework Programme (FP7), grant/award no. 211384 : European Project on Ocean Acidification

Sixth Framework Programme (FP6), grant/award no. 511106 : European network of excellence for Ocean Ecosystems Analysis

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Fiorini, Sarah
2	Sample ID	Sample ID		Fiorini, Sarah
3	Carbonate system computation flag	CSC flag		Fiorini, Sarah
4	Radiation, photosynthetically active	PAR	µmol/m²/s	Fiorini, Sarah
5	Salinity	Sal		Fiorini, Sarah
6	Temperature, water	Temp	°C	Fiorini, Sarah
7	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Fiorini, Sarah		Mean
8	pH	pH		Fiorini, Sarah		Mean, total scale
9	Emiliania huxleyi	E. huxleyi	#/l	Fiorini, Sarah
10	Calcidiscus leptoporus	C. leptoporus	#/l	Fiorini, Sarah
11	Syracosphaera pulchra	S. pulchra	#/l	Fiorini, Sarah
12	Growth rate	µ	#/day	Fiorini, Sarah
13	Carbon, inorganic, dissolved	DIC	µmol/kg	Fiorini, Sarah	Coulometric titration	Mean
14	Alkalinity, total	AT	µmol/kg	Fiorini, Sarah	Titration potentiometric	Mean
15	Carbon dioxide	CO2	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)	Mean
16	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)	Mean
17	Bicarbonate ion	[HCO3]-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)	Mean
18	Carbonate ion	[CO3]2-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)	Mean
19	Aragonite saturation state	Omega Arg		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)	Mean
20	Calcite saturation state	Omega Cal		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)	Mean
21	Carbon, total, particulate	TPC	%	Fiorini, Sarah	Mass spectrometer Thermo Electron Flash EA 1122 Analyzer
22	Carbon, total, particulate	TPC	µg/l	Fiorini, Sarah	Mass spectrometer Thermo Electron Flash EA 1122 Analyzer
23	Total particulate carbon per cell	TPC/cell	pg/#	Fiorini, Sarah	Mass spectrometer Thermo Electron Flash EA 1122 Analyzer
24	Carbon, organic, particulate	POC	%	Fiorini, Sarah	Mass spectrometer Thermo Electron Flash EA 1122 Analyzer
25	Carbon, organic, particulate	POC	µg/l	Fiorini, Sarah	Mass spectrometer Thermo Electron Flash EA 1122 Analyzer
26	Carbon, organic, particulate, per cell	POC/cell	pg/#	Fiorini, Sarah	Mass spectrometer Thermo Electron Flash EA 1122 Analyzer
27	Carbon, inorganic, particulate, per cell	PIC/cell	pg/#	Fiorini, Sarah	Calculated
28	δ13C, particulate organic carbon	δ13C POC	‰ PDB	Fiorini, Sarah
29	δ13C, dissolved inorganic carbon	δ13C DIC	‰ PDB	Fiorini, Sarah
30	Nitrogen, total	TN	%	Fiorini, Sarah
31	Nitrogen	N	µg/l	Fiorini, Sarah
32	Nitrogen per cell	N/cell	pg/#	Fiorini, Sarah
33	Carbon/Nitrogen ratio	C/N		Fiorini, Sarah

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

2170 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Species	2 Sample ID	3 CSC flag	4 PAR [µmol/m²/s]	5 Sal	6 Temp [°C]	7 pCO2water_SST_wet [µatm]	8 pH	9 E. huxleyi [#/l]	10 C. leptoporus [#/l]	11 S. pulchra [#/l]	12 µ [#/day]	13 DIC [µmol/kg]	14 AT [µmol/kg]	15 CO2 [µmol/kg]	16 fCO2water_SST_wet [µatm]	17 [HCO3]- [µmol/kg]	18 [CO3]2- [µmol/kg]	19 Omega Arg	20 Omega Cal	21 TPC [%]	22 TPC [µg/l]	23 TPC/cell [pg/#]	24 POC [%]	25 POC [µg/l]	26 POC/cell [pg/#]	27 PIC/cell [pg/#]	28 δ13C POC [‰ PDB]	29 δ13C DIC [‰ PDB]	30 TN [%]	31 N [µg/l]	32 N/cell [pg/#]	33 C/N
Emiliania huxleyi	pCO2=400 E. hux 2N 1.1	15	160	33.6	19	391.0	8.05	48267000			0.95	1986.5	2241	11.99	357.13	1793.54	180.975	2.83	4.37	7.20	1.14	25	4.590	0.000000725	16	9.0	-27.43	-15.71	0.77	0.12	2.65	6.0
Emiliania huxleyi	pCO2=400 E. hux 2N 1.2	15	160	33.6	19	391.0	8.05	48267000			0.95	1986.5	2241	11.99	357.13	1793.54	180.975	2.83	4.37	6.93	1.13	25	4.500	0.000000733	16	8.6	-27.45	-16.04	0.72	0.12	2.54	6.3
Emiliania huxleyi	pCO2=400 E. hux 2N 2.1	15	160	33.6	19	389.0	8.05	48267000			0.95	1985.0	2240	11.93	355.19	1791.48	181.595	2.83	4.38	7.32	1.24	24	4.660	0.000000792	15	8.8	-27.09	-15.89	0.77	0.13	2.52	6.1
Emiliania huxleyi	pCO2=400 E. hux 2N 2.2	15	160	33.6	19	389.0	8.05	48267000			0.95	1985.0	2240	11.93	355.19	1791.48	181.595	2.83	4.38	6.02	1.25	24	3.870	0.000000805	16	8.7	-27.17	-15.46	0.64	0.13	2.57	6.1
Emiliania huxleyi	pCO2=400 E. hux 2N 3.1	15	160	33.6	19	389.5	8.05	48267000			0.95	1976.0	2229	11.96	356.18	1784.44	179.605	2.81	4.33	6.23	1.15	24	4.130	0.000000762	16	8.2	-27.32	-15.42	0.70	0.13	2.72	5.9
Emiliania huxleyi	pCO2=400 E. hux 2N 3.2	15	160	33.6	19	389.5	8.05	48267000			0.95	1976.0	2229	11.96	356.18	1784.44	179.605	2.81	4.33	5.74	1.18	25	3.780	0.000000774	16	8.5	-27.40	-15.35	0.63	0.13	2.75	6.0
Emiliania huxleyi	pCO2=400 E. hux N 1.1	15	160	33.6	19	367.5	8.08	37091000			0.93	2007.0	2282	11.28	335.62	1800.07	195.670	3.06	4.72	4.30	0.83	25	4.250	0.000000821	24	0.3	-29.17	-15.64	0.70	0.14	4.04	6.1
Emiliania huxleyi	pCO2=400 E. hux N 1.2	15	160	33.6	19	367.5	8.08	37091000			0.93	2007.0	2282	11.28	335.62	1800.07	195.670	3.06	4.72	4.03	0.81	24	4.100	0.000000828	25	-0.4	-29.08	-15.70	0.69	0.14	4.15	6.0
Emiliania huxleyi	pCO2=400 E. hux N 2.1	15	160	33.6	19	364.0	8.08	37091000			0.93	1996.0	2271	11.18	332.67	1789.57	195.265	3.04	4.71	5.17	0.71	19	5.040	0.000000692	18	0.5	-29.14	-15.54	0.83	0.11	3.01	6.1
Emiliania huxleyi	pCO2=400 E. hux N 2.2	15	160	33.6	19	364.0	8.08	37091000			0.93	1996.0	2271	11.18	332.67	1789.57	195.265	3.04	4.71	4.60	0.89	23	4.540	0.000000879	23	0.3	-28.94	-15.41	0.77	0.15	3.91	5.9
Emiliania huxleyi	pCO2=400 E. hux N 3.1	15	160	33.6	19	363.5	8.08	37091000			0.93	1983.0	2256	11.12	330.94	1778.13	193.755	3.02	4.67	4.40	0.82	21	4.420	0.000000825	21	-0.1	-28.83	-15.55	0.77	0.14	3.62	5.7
Emiliania huxleyi	pCO2=400 E. hux N 3.2	15	160	33.6	19	363.5	8.08	37091000			0.93	1983.0	2256	11.12	330.94	1778.13	193.755	3.02	4.67	4.43	0.82	21	4.330	0.000000798	20	0.5	-28.96	-15.36	0.75	0.14	3.49	5.8
Emiliania huxleyi	pCO2=760 E. hux 2N 1.1	15	160	33.9	19	759.5	7.80	33199000			0.98	2087.5	2220	24.45	729.52	1956.67	106.370	1.66	2.56	4.28	0.84	25	2.500	0.000000489	15	10.6	-33.74	-4.84	0.42	0.08	2.47	6.0
Emiliania huxleyi	pCO2=760 E. hux 2N 1.2	15	160	33.9	19	759.5	7.80	33199000			0.98	2087.5	2220	24.45	729.52	1956.67	106.370	1.66	2.56	3.83	0.83	25	2.300	0.000000500	15	10.2	-33.94	-5.50	0.39	0.08	2.55	6.0
Emiliania huxleyi	pCO2=760 E. hux 2N 2.1	15	160	33.9	19	761.0	7.80	33199000			0.98	2086.5	2219	24.50	731.03	1955.95	106.040	1.66	2.55	3.73	0.82	26	2.180	0.000000480	15	10.9	-34.07	-4.84	0.42	0.09	2.99	5.2
Emiliania huxleyi	pCO2=760 E. hux 2N 2.2	15	160	33.9	19	761.0	7.80	33199000			0.98	2086.5	2219	24.50	731.03	1955.95	106.040	1.66	2.55	3.47	0.80	26	2.210	0.000000507	16	9.3	-34.10	-4.73	0.43	0.10	3.15	5.2
Emiliania huxleyi	pCO2=760 E. hux 2N 3.1	15	160	33.9	19	758.0	7.80	33199000			0.98	2086.5	2219	24.50	731.03	1955.95	106.040	1.66	2.55	3.79	0.81	23	2.310	0.000000495	14	8.9	-34.33	-4.88	0.45	0.10	2.73	5.1
Emiliania huxleyi	pCO2=760 E. hux 2N 3.2	15	160	33.9	19	758.0	7.80	33199000			0.98	2086.5	2219	24.50	731.03	1955.95	106.040	1.66	2.55	4.06	0.91	26	2.160	0.000000482	14	12.0	-34.29	-5.13	0.42	0.09	2.66	5.1
Emiliania huxleyi	pCO2=760 E. hux N 1.1	15	160	33.9	19	719.0	7.83	24430000			0.94	2104.0	2249	23.20	692.10	1966.29	114.510	1.78	2.75	2.10	0.41	16	2.080	0.000000408	16	0.1	-35.32	-4.87	0.42	0.08	3.17	5.0
Emiliania huxleyi	pCO2=760 E. hux N 1.2	15	160	33.9	19	719.0	7.83	24430000			0.94	2104.0	2249	23.20	692.10	1966.29	114.510	1.78	2.75	2.11	0.43	17	2.070	0.000000422	16	0.3	-35.43	-5.42	0.41	0.08	3.24	5.0
Emiliania huxleyi	pCO2=760 E. hux N 2.1	15	160	33.9	19	724.0	7.83	24430000			0.94	2103.0	2247	23.36	696.62	1966.10	113.545	1.77	2.73	1.96	0.40	17	1.920	0.000000397	17	0.3	-35.08	-5.75	0.40	0.08	3.44	4.8
Emiliania huxleyi	pCO2=760 E. hux N 2.2	15	160	33.9	19	724.0	7.83	24430000			0.94	2103.0	2247	23.36	696.62	1966.10	113.545	1.77	2.73	2.13	0.43	18	2.090	0.000000420	18	0.3	-35.05	-5.51	0.42	0.09	3.55	4.9
Emiliania huxleyi	pCO2=760 E. hux N 3.1	15	160	33.9	19	719.5	7.83	24430000			0.94	2091.5	2234	23.29	694.69	1955.62	112.595	1.75	2.71	2.06	0.46	20	2.050	0.000000458	19	0.1	-34.73	-4.94	0.42	0.10	4.04	4.8
Emiliania huxleyi	pCO2=760 E. hux N 3.2	15	160	33.9	19	719.5	7.83	24430000			0.94	2091.5	2234	23.29	694.69	1955.62	112.595	1.75	2.71	2.20	0.41	17	2.230	0.000000413	18	-0.2	-34.70	-4.94	0.47	0.09	3.66	4.8
Calcidiscus leptoporus	pCO2=400 C. lep 2N 1.1	15	160	33.6	19	437.8	8.00		5061000		0.33	1927.5	2150	13.33	396.95	1755.71	158.460	2.47	3.83	4.22	0.98	171	1.710	0.000000395	69	101.7	-33.00	-10.25	0.37	0.08	14.83	4.7
Calcidiscus leptoporus	pCO2=400 C. lep 2N 1.2	15	160	33.6	19	437.8	8.00		5061000		0.33	1927.5	2150	13.33	396.95	1755.71	158.460	2.47	3.83	4.94	0.98	172	2.170	0.000000430	75	96.5	-32.84	-9.64	0.43	0.09	15.03	5.0
Calcidiscus leptoporus	pCO2=400 C. lep 2N 2.1	15	160	33.6	19	443.8	7.99		5061000		0.33	1927.5	2148	13.53	402.92	1757.09	156.885	2.44	3.79	5.53	1.01	214	2.170	0.000000396	84	129.7	-32.72	-9.30	0.43	0.08	16.61	5.1
Calcidiscus leptoporus	pCO2=400 C. lep 2N 2.2	15	160	33.6	19	443.8	7.99		5061000		0.33	1927.5	2148	13.53	402.92	1757.09	156.885	2.44	3.79	5.01	1.00	213	1.970	0.000000395	84	129.1	-32.78	-9.68	0.40	0.08	16.93	5.0
Calcidiscus leptoporus	pCO2=400 C. lep 2N 3.1	15	160	33.6	19	441.2	8.00		5061000		0.33	1934.5	2156	13.50	402.04	1762.95	158.050	2.46	3.81	4.36	0.96	202	1.770	0.000000391	82	119.8	-32.57	-11.00	0.36	0.08	16.85	4.9
Calcidiscus leptoporus	pCO2=400 C. lep 2N 3.2	15	160	33.6	19	441.2	8.00		5061000		0.33	1934.5	2156	13.50	402.04	1762.95	158.050	2.46	3.81	4.28	0.96	202	2.090	0.000000468	98	103.5	-32.75	-14.40	0.37	0.08	17.30	5.7
Calcidiscus leptoporus	pCO2=400 C. lep N 1.1	15	160	33.6	19	372.5	8.07		9268000		0.54	1954.5	2214	11.40	339.62	1759.89	183.205	2.85	4.42	4.13	0.74	74	4.060	0.000000729	73	1.2	-31.69	-9.69	0.46	0.08	8.18	8.9
Calcidiscus leptoporus	pCO2=400 C. lep N 1.2	15	160	33.6	19	372.5	8.07		9268000		0.54	1954.5	2214	11.40	339.62	1759.89	183.205	2.85	4.42	3.49	0.59	60	3.390	0.000000579	58	1.6	-30.91	-9.81	0.44	0.07	7.50	7.7
Calcidiscus leptoporus	pCO2=400 C. lep N 2.1	15	160	33.6	19	378.8	8.06		9268000		0.54	1951.5	2207	11.57	344.53	1759.51	180.420	2.81	4.36	2.30	0.37	42	2.180	0.000000348	40	2.3	-31.34	-9.73	0.38	0.06	6.88	5.8
Calcidiscus leptoporus	pCO2=400 C. lep N 2.2	15	160	33.6	19	378.8	8.06		9268000		0.54	1951.5	2207	11.57	344.53	1759.51	180.420	2.81	4.36	2.41	0.37	42	2.550	0.000000392	45	-2.4	-31.36	-9.80	0.46	0.07	8.01	5.6
Calcidiscus leptoporus	pCO2=400 C. lep N 3.1	15	160	33.6	19	373.1	8.06		9268000		0.54	1949.5	2210	11.29	336.05	1754.18	184.035	2.87	4.44	2.06	0.36	39	2.290	0.000000396	44	-4.4	-31.13	-9.93	0.40	0.07	7.64	5.7
Calcidiscus leptoporus	pCO2=400 C. lep N 3.2	15	160	33.6	19	373.1	8.06		9268000		0.54	1949.5	2210	11.29	336.05	1754.18	184.035	2.87	4.44	3.00	0.43	47	2.760	0.000000393	43	3.8	-31.43	-9.86	0.45	0.06	7.06	6.2
Calcidiscus leptoporus	pCO2=760 C. lep 2N 1.1	15	160	34.5	19	866.8	7.76		6162000		0.42	2143.0	2272	26.46	791.90	2011.11	105.435	1.64	2.52	4.83	1.00	164	2.480	0.000000512	84	80.0	-35.55	-14.53	0.55	0.11	18.58	4.5
Calcidiscus leptoporus	pCO2=760 C. lep 2N 1.2	15	160	34.5	19	866.8	7.76		6162000		0.42	2143.0	2272	26.46	791.90	2011.11	105.435	1.64	2.52	5.06	1.10	181	2.180	0.000000474	78	103.2	-35.78	-14.46	0.47	0.10	16.90	4.6
Calcidiscus leptoporus	pCO2=760 C. lep 2N 3.1	15	160	34.5	19	866.9	7.76		6162000		0.42	2128.5	2256	26.46	791.83	1997.85	104.195	1.61	2.50	5.31	1.11	178	2.450	0.000000514	82	96.0	-35.30	-14.16	0.53	0.11	17.74	4.6
Calcidiscus leptoporus	pCO2=760 C. lep 2N 3.2	15	160	34.5	19	866.8	7.76		6162000		0.42	2128.5	2256	26.46	791.83	1997.85	104.195	1.61	2.50	5.65	1.15	184	2.460	0.000000501	80	103.7	-35.28	-13.99	0.51	0.10	16.69	4.8
Calcidiscus leptoporus	pCO2=760 C. lep N 1.1	15	160	34.5	19	736.1	7.83		5965000		0.63	2167.0	2326	22.50	673.26	2020.05	124.450	1.93	2.98	6.25	0.34	54	6.340	0.000000341	55	-0.8	-34.96	-14.20	1.05	0.06	9.04	6.1
Calcidiscus leptoporus	pCO2=760 C. lep N 1.2	15	160	34.5	19	736.1	7.83		5965000		0.63	2167.0	2326	22.50	673.26	2020.05	124.450	1.93	2.98	5.97	0.35	57	5.850	0.000000348	56	1.1	-34.66	-13.73	0.99	0.06	9.44	5.9
Calcidiscus leptoporus	pCO2=760 C. lep N 2.1	15	160	34.5	19	723.6	7.84		5965000		0.63	2169.0	2333	22.05	659.72	2019.60	127.360	1.98	3.05	6.10	0.35	58	6.310	0.000000361	60	-2.0	-35.62	-13.85	1.00	0.06	9.57	6.3
Calcidiscus leptoporus	pCO2=760 C. lep N 2.2	15	160	34.5	19	723.6	7.84		5965000		0.63	2169.0	2333	22.05	659.72	2019.60	127.360	1.98	3.05	5.33	0.37	61	5.180	0.000000356	59	1.7	-35.64	-13.50	0.81	0.06	9.31	6.4
Calcidiscus leptoporus	pCO2=760 C. lep N 3.1	15	160	34.5	19	739.9	7.83		5965000		0.63	2184.0	2345	22.59	676.11	2035.52	125.885	1.96	3.02	5.86	0.35	62	5.720	0.000000343	60	1.4	-35.55	-14.36	0.96	0.06	10.18	5.9
Calcidiscus leptoporus	pCO2=760 C. lep N 3.2	15	160	34.5	19	739.9	7.83		5965000		0.63	2184.0	2345	22.59	676.11	2035.52	125.885	1.96	3.02	4.97	0.36	64	5.070	0.000000368	65	-1.3	-35.30	-14.39	0.82	0.06	10.45	6.2
Syracosphaera pulchra	pCO2=400 S. pul 2N 1.1	15	160	33.8	19	377.5	8.06			6186000	0.62	1952.5	2212	11.47	341.99	1757.74	183.280	2.85	4.42	6.13	1.12	171	4.920	0.000000902	138	33.8	-29.53	-9.19	0.75	0.14	21.06	6.5
Syracosphaera pulchra	pCO2=400 S. pul 2N 1.2	15	160	33.8	19	377.5	8.06			6186000	0.62	1952.5	2212	11.47	341.99	1757.74	183.280	2.85	4.42	5.52	1.10	168	4.480	0.000000893	136	31.6	-29.59	-9.11	0.69	0.14	21.07	6.5
Syracosphaera pulchra	pCO2=400 S. pul 2N 2.1	15	160	33.8	19	384.9	8.06			6186000	0.62	1960.5	2218	11.65	347.58	1766.95	181.890	2.83	4.38	5.09	0.95	182	4.170	0.000000777	149	32.9	-28.87	-9.68	0.63	0.12	22.64	6.6
Syracosphaera pulchra	pCO2=400 S. pul 2N 2.2	15	160	33.8	19	384.9	8.06			6186000	0.62	1960.5	2218	11.65	347.58	1766.95	181.890	2.83	4.38	4.80	1.19	228	3.670	0.000000905	174	53.9	-29.88	-9.18	0.53	0.13	25.18	6.9
Syracosphaera pulchra	pCO2=400 S. pul 2N 3.1	15	160	33.8	19	376.9	8.06			6186000	0.62	1957.0	2218	11.47	341.97	1761.40	184.120	2.87	4.44	4.73	1.10	162	4.280	0.000000998	147	15.4	-28.73	-9.50	0.63	0.15	21.66	6.8
Syracosphaera pulchra	pCO2=400 S. pul 2N 3.2	15	160	33.8	19	376.9	8.06			6186000	0.62	1957.0	2218	11.47	341.97	1761.40	184.120	2.87	4.44	5.25	0.90	132	4.760	0.000000814	120	12.2	-29.07	-9.20	0.75	0.13	18.75	6.4
Syracosphaera pulchra	pCO2=400 S. pul N 1.1	15	160	33.8	19	379.9	8.06			3934000	0.53	1972.0	2234	11.57	344.93	1775.09	185.340	2.89	4.46	3.62	0.81	203	3.480	0.000000776	195	7.8	-31.48	-9.16	0.52	0.12	29.41	6.6
Syracosphaera pulchra	pCO2=400 S. pul N 1.2	15	160	33.8	19	379.9	8.06			3934000	0.53	1972.0	2234	11.57	344.93	1775.09	185.340	2.89	4.46	3.49	0.80	201	3.620	0.000000828	209	-7.8	-31.31	-9.05	0.54	0.12	31.12	6.7
Syracosphaera pulchra	pCO2=400 S. pul N 2.1	15	160	33.8	19	378.8	8.06			3934000	0.53	1968.5	2230	11.55	344.58	1772.07	184.875	2.88	4.46	4.09	0.79	200	4.140	0.000000804	203	-2.5	-30.87	-9.12	0.63	0.12	31.00	6.5
Syracosphaera pulchra	pCO2=400 S. pul N 2.2	15	160	33.8	19	378.8	8.06			3934000	0.53	1968.5	2230	11.55	344.58	1772.07	184.875	2.88	4.46	5.06	0.81	205	4.930	0.000000792	200	5.6	-31.26	-9.17	0.73	0.12	29.70	6.7
Syracosphaera pulchra	pCO2=400 S. pul N 3.1	15	160	33.8	19	376.8	8.07			3934000	0.53	1964.5	2226	11.52	343.44	1768.24	184.730	2.88	4.45	3.43	0.78	203	3.410	0.000000780	201	1.2	-30.90	-9.13	0.52	0.12	30.69	6.6
Syracosphaera pulchra	pCO2=400 S. pul N 3.2	15	160	33.8	19	376.8	8.06			3934000	0.53	1964.5	2226	11.52	343.44	1768.24	184.730	2.88	4.45	3.49	0.78	202	3.510	0.000000784	203	-0.9	-31.03	-9.06	0.51	0.11	29.37	6.9
Syracosphaera pulchra	pCO2=760 S. pul 2N 1.1	15	160	33.9	19	665.6	7.85			7655000	0.66	2067.0	2229	20.39	608.00	1922.89	123.725	1.93	2.98	4.23	0.94	127	3.780	0.000000836	113	13.7	-33.66	-17.79	0.70	0.15	20.98	5.4
Syracosphaera pulchra	pCO2=760 S. pul 2N 1.2	15	160	33.9	19	665.6	7.85			7655000	0.66	2067.0	2229	20.39	608.00	1922.89	123.725	1.93	2.98	5.75	0.92	125	4.520	0.000000722	98	26.8	-33.66	-15.44	0.84	0.13	18.27	5.3
Syracosphaera pulchra	pCO2=760 S. pul 2N 2.1	15	160	33.9	19	681.2	7.85			7655000	0.66	2076.5	2235	20.88	622.72	1933.82	121.805	1.90	2.94	4.71	0.98	128	3.560	0.000000744	96	31.1	-32.95	-15.14	0.68	0.14	18.55	5.2
Syracosphaera pulchra	pCO2=760 S. pul 2N 2.2	15	160	33.9	19	681.2	7.85			7655000	0.66	2076.5	2235	20.88	622.72	1933.82	121.805	1.90	2.94	3.84	0.91	117	3.210	0.000000757	98	19.3	-33.68	-14.97	0.61	0.14	18.57	5.3
Syracosphaera pulchra	pCO2=760 S. pul 2N 3.1	15	160	33.9	19	676.0	7.85			7655000	0.66	2074.0	2234	20.68	616.99	1930.64	122.675	1.91	2.96	3.97	0.88	112	3.320	0.000000735	93	18.1	-33.61	-14.94	0.62	0.14	17.32	5.4
Syracosphaera pulchra	pCO2=760 S. pul 2N 3.2	15	160	33.9	19	676.0	7.85			7655000	0.66	2074.0	2234	20.68	616.99	1930.64	122.675	1.91	2.96	3.92	0.92	117	3.120	0.000000730	93	23.8	-33.71	-14.86	0.58	0.14	17.29	5.4
Syracosphaera pulchra	pCO2=760 S. pul N 1.1	15	160	33.9	19	579.1	7.92			10107000	0.61	2050.5	2244	17.80	530.79	1889.40	143.300	2.23	3.45	4.22	1.01	94	4.180	0.000001001	94	0.8	-38.63	-14.16	0.74	0.18	16.53	5.7
Syracosphaera pulchra	pCO2=760 S. pul N 1.2	15	160	33.9	19	579.1	7.92			10107000	0.61	2050.5	2244	17.80	530.79	1889.40	143.300	2.23	3.45	6.23	1.04	98	6.140	0.000001029	96	1.4	-38.64	-14.12	1.06	0.18	16.61	5.8
Syracosphaera pulchra	pCO2=760 S. pul N 2.1	15	160	33.9	19	606.0	7.90			10107000	0.61	2064.0	2248	18.57	553.80	1907.96	137.470	2.14	3.31	4.82	0.90	98	4.800	0.000000901	98	0.4	-36.59	-14.52	0.88	0.17	18.01	5.4
Syracosphaera pulchra	pCO2=760 S. pul N 2.2	15	160	33.9	19	606.0	7.90			10107000	0.61	2064.0	2248	18.57	553.80	1907.96	137.470	2.14	3.31	4.76	0.91	99	4.740	0.000000909	99	0.5	-38.01	-14.40	0.82	0.16	17.01	5.8
Syracosphaera pulchra	pCO2=760 S. pul N 3.1	15	160	33.9	19	595.1	7.91			10107000	0.61	2062.0	2251	18.21	543.08	1903.10	140.695	2.19	3.39	4.50	0.98	94	4.500	0.000000976	94	0.0	-38.17	-14.48	0.79	0.17	16.42	5.7
Syracosphaera pulchra	pCO2=760 S. pul N 3.2	15	160	33.9	19	595.1	7.91			10107000	0.61	2062.0	2251	18.21	543.08	1903.10	140.695	2.19	3.39	5.73	0.99	95	5.690	0.000000984	95	0.6	-38.01	-14.44	1.00	0.17	16.58	5.7