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Sciandra, Antoine; Harlay, Jérôme; Lefèvre, Dominique; Lemee, R; Rimmelin, Peguy; Denis, Michel; Gattuso, Jean-Pierre (2003): Seawater carbonate chemistry and processes during experiments with Emiliania huxleyi (TW1), 2003. PANGAEA, https://doi.org/10.1594/PANGAEA.727841, Supplement to: Sciandra, A et al. (2003): Response of coccolithophorid Emiliania huxleyi to elevated partial pressure of CO2 under nitrogen limitation. Marine Ecology Progress Series, 261, 111-122, https://doi.org/10.3354/meps261111

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Abstract:
Precipitation of calcium carbonate by phytoplankton in the photic oceanic layer is an important process regulating the carbon cycling and the exchange of CO2 at the ocean-atmosphere interface. Previous experiments have demonstrated that, under nutrient-sufficient conditions, doubling the partial pressure of CO2 (pCO2) in seawater-a likely scenario for the end of the century-can significantly decrease both the rate of calcification by coccolithophorids and the ratio of inorganic to organic carbon production. The present work investigates the effects of high pCO2 on calcification by the coccolithophore Emiliania huxleyi (Strain TW1) grown under nitrogen-limiting conditions, a situation that can also prevail in the ocean. Nitrogen limitation was achieved in NO3-limited continuous cultures renewed at the rate of 0.5 d-1 and exposed to a saturating light level. pCO2 was increased from 400 to 700 ppm and controlled by bubbling CO2-rich or CO2-free air into the cultures. The pCO2 shift has a rapid effect on cell physiology that occurs within 2 cell divisions subsequent to the perturbation. Net calcification rate (C) decreased by 25% and, in contrast to previous studies with N-replete cultures, gross community production (GCP) and dark community respiration (DCR) also decreased. These results suggest that increasing pCO2 has no noticeable effect on the calcification/photosynthesis ratio (C/P) when cells of E. huxleyi are NO3-limited.
Coverage:
Date/Time Start: 2001-10-29T09:00:00 * Date/Time End: 2001-11-24T08:00:00
Event(s):
Sciandra_etal_03 * Device: Experiment (EXP)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1Experimental treatmentExp treatSciandra, Antoine
2DATE/TIMEDate/TimeGeocode
3Experiment dayExp daydaySciandra, Antoine
4SalinitySalSciandra, Antoine
5Temperature, waterTemp°CSciandra, Antoine
6Radiation, photosynthetically activePARµmol/m2/sSciandra, AntoineMeasured
7Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
8pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale
9Alkalinity, totalATmmol(eq)/lSciandra, AntoineAlkalinity, Gran titration (Gran, 1950)
10Alkalinity, totalATµmol/kgSciandra, AntoineCalculatedOriginal value in mEq/l, calculated using density
11Carbon, inorganic, dissolvedDICµmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
12Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
13Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmSciandra, AntoineMeasured
14Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
15Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
16Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
17Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
18Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
19Calcification rate of calcium carbonate per algae cellCalc rate CaCO3pmol/#/daySciandra, AntoineCalculated, see reference(s)
20Production of particulate organic carbon per cellPOC prodpg/#/daySciandra, AntoineElement analyser CHN, LECO
21Particulate inorganic carbon production per cellPIC prodpg/#/daySciandra, AntoineCalculated
Size:
941 data points

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