Data Description

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Citation:
Czerny, J et al. (2009): Seawater carbonate chemistry and processes during experiments with cyanobacterium Nodularia spumigena, 2009. doi:10.1594/PANGAEA.726862,
Supplement to: Czerny, Jan; Barcelos e Ramos, Joana; Riebesell, Ulf (2009): Influence of elevated CO2 concentrations on cell division and nitrogen fixation rates in the bloom-forming cyanobacterium Nodularia spumigena. Biogeosciences, 6(9), 1865-1875, doi:10.5194/bg-6-1865-2009
Abstract:
The surface ocean absorbs large quantities of the CO2 emitted to the atmosphere from human activities. As this CO2 dissolves in seawater, it reacts to form carbonic acid. While this phenomenon, called ocean acidification, has been found to adversely affect many calcifying organisms, some photosynthetic organisms appear to benefit from increasing [CO2]. Among these is the cyanobacterium Trichodesmium, a predominant diazotroph (nitrogen-fixing) in large parts of the oligotrophic oceans, which responded with increased carbon and nitrogen fixation at elevated pCO2. With the mechanism underlying this CO2 stimulation still unknown, the question arises whether this is a common response of diazotrophic cyanobacteria. In this study we therefore investigate the physiological response of Nodularia spumigena, a heterocystous bloom-forming diazotroph of the Baltic Sea, to CO2-induced changes in seawater carbonate chemistry. N. spumigena reacted to seawater acidification/carbonation with reduced cell division rates and nitrogen fixation rates, accompanied by significant changes in carbon and phosphorus quota and elemental composition of the formed biomass. Possible explanations for the contrasting physiological responses of Nodularia compared to Trichodesmium may be found in the different ecological strategies of non-heterocystous (Trichodesmium) and heterocystous (Nodularia) cyanobacteria.
Project(s):
Event(s):
Czerny_etal_09 * * Device: Experiment (EXP) *
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1Experimental treatment *Exp trtmCzerny, Jan *
2Salinity *SalCzerny, Jan *
3Temperature, water *Temp°CCzerny, Jan *
4Radiation, photosynthetically active *PARµE/m2/sCzerny, Jan *
5pH *pHCzerny, Jan *Calculated using CO2SYS *Free scale, constants (K1 and K2) by Roy et al. (1993)
6pH *pHNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *Total scale
7Carbon dioxide *CO2µmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
8Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
9Fugacity of carbon dioxide (water) at sea surface temperature (wet air) *fCO2water_SST_wetµatmNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
10Bicarbonate ion *[HCO3]-µmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
11Carbonate ion *[CO3]2-µmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
12Carbon, inorganic, dissolved *DICµmol/kgCzerny, Jan *Automated segmented-flow analyzer (Quaatro) *Mean from start to end, constants (K1 and K2) by Roy et al. (1993)
13Alkalinity, total *ATµmol/kgCzerny, Jan *Titration potentiometric *Constants (K1 and K2) by Roy et al. (1993)
14Aragonite saturation state *Omega ArgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
15Calcite saturation state *Omega CalNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
16Carbon, inorganic, dissolved *DICµmol/kgCzerny, Jan *Automated segmented-flow analyzer (Quaatro) *Start
17Carbon, inorganic, dissolved *DICµmol/kgCzerny, Jan *Automated segmented-flow analyzer (Quaatro) *End
18Carbon, inorganic, dissolved *DICµmol/kgCzerny, Jan *Calculated *Consumption, constants (K1 and K2) by Roy et al. (1993)
19Duration, number of days *DurationdaysCzerny, Jan *
20Chlorophyll a *Chl aµg/lCzerny, Jan *Fluorometry *Start
21Chlorophyll a *Chl aµg/lCzerny, Jan *Fluorometry *End
22Phosphorus, organic, particulate *POPµmol/lCzerny, Jan *Spectrophotometer Hitachi U-2000 *
23Phosphorus, organic, particulate *POPµgCzerny, Jan *Calculated *In cell
24Production of particulate organic phosphorus, per cell *POP prodpmol/#/dayCzerny, Jan *Calculated *
25Phosphate *PO4µmol/lCzerny, Jan *Calculated *Consumption during the experiment
26Nitrogen, organic, particulate *PONµmol/lCzerny, Jan *Element analyser CNS, EURO EA *
27Nitrogen per cell *N cellpmolCzerny, Jan *
28Production of particulate organic nitrogen *PON prodpg/#/dayCzerny, Jan *
29Carbon, organic, particulate *POCµmol/lCzerny, Jan *End
30Carbon per cell *C cellpmolCzerny, Jan *
31Production of particulate organic carbon per cell *POC prodpg/#/dayCzerny, Jan *Calculated *
32Nodularia spumigena *N. spumigena#/lCzerny, Jan *Counting from image *Start
33Nodularia spumigena *N. spumigena#/lCzerny, Jan *Counting from image *End
34Cell division rate *Cell division#/dayCzerny, Jan *
35Acetylene reduction *C2H2 reductionµmol/l/hCzerny, Jan *
36Nitrogen fixation rate, per cell *N2 fixfmol/#/hCzerny, Jan *Calculated *Calculated using a convertion factor of 3
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