Citation:

bg-6-1865-2009

Name: Seawater carbonate chemistry and processes during experiments with cyanobacterium Nodularia spumigena, 2009
Published: 2009-08-27
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Bacteria; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Cyanobacteria; Laboratory experiment; Laboratory strains; Nodularia spumigena; Not applicable; Other metabolic rates; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species

Always quote citation above when using data! You can download the citation in several formats below.

RIS Citation BibTeX Citation Text Citation Facebook Twitter

Abstract:

The surface ocean absorbs large quantities of the CO2 emitted to the atmosphere from human activities. As this CO2 dissolves in seawater, it reacts to form carbonic acid. While this phenomenon, called ocean acidification, has been found to adversely affect many calcifying organisms, some photosynthetic organisms appear to benefit from increasing [CO2]. Among these is the cyanobacterium Trichodesmium, a predominant diazotroph (nitrogen-fixing) in large parts of the oligotrophic oceans, which responded with increased carbon and nitrogen fixation at elevated pCO2. With the mechanism underlying this CO2 stimulation still unknown, the question arises whether this is a common response of diazotrophic cyanobacteria. In this study we therefore investigate the physiological response of Nodularia spumigena, a heterocystous bloom-forming diazotroph of the Baltic Sea, to CO2-induced changes in seawater carbonate chemistry. N. spumigena reacted to seawater acidification/carbonation with reduced cell division rates and nitrogen fixation rates, accompanied by significant changes in carbon and phosphorus quota and elemental composition of the formed biomass. Possible explanations for the contrasting physiological responses of Nodularia compared to Trichodesmium may be found in the different ecological strategies of non-heterocystous (Trichodesmium) and heterocystous (Nodularia) cyanobacteria.

Keyword(s):

Bacteria; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Cyanobacteria; Laboratory experiment; Laboratory strains; Nodularia spumigena; Not applicable; Other metabolic rates; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species

Project(s):

European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)

European Project on Ocean Acidification (EPOCA)

Ocean Acidification International Coordination Centre (OA-ICC)

Surface Ocean Processes in the Anthropocene (SOPRAN)

Funding:

Seventh Framework Programme (FP7), grant/award no. 211384 : European Project on Ocean Acidification

Sixth Framework Programme (FP6), grant/award no. 511106 : European network of excellence for Ocean Ecosystems Analysis

Event(s):

Czerny_etal_09 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Experimental treatment	Exp treat		Czerny, Jan
2	Salinity	Sal		Czerny, Jan
3	Temperature, water	Temp	°C	Czerny, Jan
4	Radiation, photosynthetically active	PAR	µmol/m²/s	Czerny, Jan
5	pH	pH		Czerny, Jan	Calculated using CO2SYS	Free scale, constants (K1 and K2) by Roy et al. (1993)
6	pH	pH		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)	Total scale
7	Carbon dioxide	CO2	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
8	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
9	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
10	Bicarbonate ion	[HCO3]-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
11	Carbonate ion	[CO3]2-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
12	Carbon, inorganic, dissolved	DIC	µmol/kg	Czerny, Jan	Automated segmented-flow analyzer (Quaatro)	Mean from start to end, constants (K1 and K2) by Roy et al. (1993)
13	Alkalinity, total	AT	µmol/kg	Czerny, Jan	Titration potentiometric	Constants (K1 and K2) by Roy et al. (1993)
14	Aragonite saturation state	Omega Arg		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
15	Calcite saturation state	Omega Cal		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
16	Carbon, inorganic, dissolved	DIC	µmol/kg	Czerny, Jan	Automated segmented-flow analyzer (Quaatro)	Start
17	Carbon, inorganic, dissolved	DIC	µmol/kg	Czerny, Jan	Automated segmented-flow analyzer (Quaatro)	End
18	Carbon, inorganic, dissolved	DIC	µmol/kg	Czerny, Jan	Calculated	Consumption, constants (K1 and K2) by Roy et al. (1993)
19	Duration, number of days	Duration	days	Czerny, Jan
20	Chlorophyll a	Chl a	µg/l	Czerny, Jan	Fluorometry	Start
21	Chlorophyll a	Chl a	µg/l	Czerny, Jan	Fluorometry	End
22	Phosphorus, organic, particulate	POP	µmol/l	Czerny, Jan	Spectrophotometer Hitachi U-2000
23	Phosphorus, organic, particulate	POP	µg	Czerny, Jan	Calculated	In cell
24	Particulate organic phosphorus production per cell	POP prod/cell	pmol/#/day	Czerny, Jan	Calculated
25	Phosphate	[PO4]3-	µmol/l	Czerny, Jan	Calculated	Consumption during the experiment
26	Nitrogen, organic, particulate	PON	µmol/l	Czerny, Jan	Element analyser CNS, EURO EA
27	Nitrogen per cell	N/cell	pmol/#	Czerny, Jan
28	Production of particulate organic nitrogen	PON prod	pg/#/day	Czerny, Jan
29	Carbon, organic, particulate	POC	µmol/l	Czerny, Jan		End
30	Carbon per cell	C/cell	pmol/#	Czerny, Jan
31	Particulate organic carbon production per cell	POC prod/cell	pg/#/day	Czerny, Jan	Calculated
32	Nodularia spumigena	N. spumigena	#/l	Czerny, Jan	Counting from image	Start
33	Nodularia spumigena	N. spumigena	#/l	Czerny, Jan	Counting from image	End
34	Cell division rate	Cell division	#/day	Czerny, Jan
35	Acetylene reduction	C2H2 reduction	µmol/l/h	Czerny, Jan
36	Nitrogen fixation rate, per cell	N2 fix/cell	fmol/#/h	Czerny, Jan	Calculated	Calculated using a convertion factor of 3

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

614 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Exp treat	2 Sal	3 Temp [°C]	4 PAR [µmol/m²/s]	5 pH (Free scale, constants (K1 and...)	6 pH (Total scale, Calculated using...)	7 CO2 [µmol/kg] (Calculated using seacarb afte...)	8 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	9 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	10 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	11 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	12 DIC [µmol/kg] (Mean from start to end, const...)	13 AT [µmol/kg] (Constants (K1 and K2) by Roy ...)	14 Omega Arg (Calculated using seacarb afte...)	15 Omega Cal (Calculated using seacarb afte...)	16 DIC [µmol/kg] (Start, Automated segmented-fl...)	17 DIC [µmol/kg] (End, Automated segmented-flow...)	18 DIC [µmol/kg] (Consumption, constants (K1 an...)	19 Duration [days]	20 Chl a [µg/l] (Start, Fluorometry)	21 Chl a [µg/l] (End, Fluorometry)	22 POP [µmol/l] (Spectrophotometer Hitachi U-2000)	23 POP [µg] (In cell, Calculated)	24 POP prod/cell [pmol/#/day] (Calculated)	25 [PO4]3- [µmol/l] (Consumption during the experi...)	26 PON [µmol/l] (Element analyser CNS, EURO EA)	27 N/cell [pmol/#]	28 PON prod [pg/#/day]	29 POC [µmol/l] (End)	30 C/cell [pmol/#]	31 POC prod/cell [pg/#/day] (Calculated)	32 N. spumigena [#/l] (Start, Counting from image)	33 N. spumigena [#/l] (End, Counting from image)	34 Cell division [#/day]	35 C2H2 reduction [µmol/l/h]	36 N2 fix/cell [fmol/#/h] (Calculated using a convertion...)
Bottle_1-pCO2=162	8	16	85	8.55	8.52	6.74	160.06	159.49	1754.4	201.83	1963	2188	3.26	5.79	1981	1946	35.4	7	1.000	16.5	0.465	0.00000088	0.451	1.20	14.4	0.880	6.33	81.55	4.969	30.62	451698	16397683	0.51	1.6200	7.792
Bottle_2-pCO2=153	8	16	85	8.57	8.55	6.33	150.39	149.86	1739.5	211.17	1957	2193	3.41	6.06	1981	1933	47.6	7	1.000	17.9	0.478	0.00000078	0.416	1.13	13.8	0.725	5.43	77.02	4.045	25.96	451698	19025144	0.53	1.5027	6.115
Bottle_3-pCO2=154	8	16	85	8.57	8.54	6.39	151.91	151.37	1741.2	209.45	1957	2191	3.38	6.01	1981	1933	47.9	7	1.000	17.7	0.478	0.00000091	0.466	1.08	15.2	0.934	6.70	81.24	4.982	30.65	451698	16291604	0.51	1.3561	6.775
Bottle_4-pCO2=297	8	16	85	8.31	8.28	12.20	289.90	288.88	1828.7	121.07	1962	2090	1.96	3.47	1981	1943	38.2	7	1.000	12.7	0.392	0.00000080	0.346	0.98	10.7	0.700	4.27	62.68	4.106	21.45	725947	15251505	0.43	0.9952	5.263
Bottle_5-pCO2=295	8	16	85	8.31	8.28	12.21	290.16	289.14	1829.7	121.09	1963	2091	1.96	3.47	1981	1945	36.0	7	1.000	13.2	0.318	0.00000083	0.330	0.94	9.2	0.771	4.31	56.27	4.727	22.68	725947	11894668	0.40	0.9875	6.887
Bottle_6-pCO2=313	8	16	85	8.29	8.26	12.81	304.26	303.19	1836.8	116.38	1966	2088	1.88	3.34	1981	1950	31.0	7	1.000	11.3	0.337	0.00000075	0.315	0.91	11.5	0.821	4.86	65.03	4.636	23.55	725947	14016005	0.42	1.0126	5.844
Bottle_7-pCO2=446	8	16	85	8.14	8.12	18.06	429.19	427.68	1866.7	85.21	1970	2051	1.38	2.45	1981	1959	22.3	7	1.000	6.9	0.253	0.00000101	0.348	0.72	7.3	0.949	4.58	44.84	5.791	23.93	696024	7737556	0.34	0.6330	6.236
Bottle_8-pCO2=435	8	16	85	8.15	8.13	17.71	420.77	419.28	1863.7	86.63	1968	2051	1.40	2.49	1981	1954	27.0	7	1.000	7.3	0.263	0.00000087	0.324	0.78	7.1	0.766	3.98	44.37	4.760	21.18	696024	9312679	0.37	0.6273	5.352
Bottle_9-pCO2=459	8	16	85	8.13	8.11	18.55	440.83	439.28	1867.4	83.02	1969	2047	1.34	2.38	1981	1957	23.7	7	1.000	7.3	0.326	0.00000107	0.399	0.69	7.9	0.835	4.35	47.52	5.050	22.56	696024	9402616	0.37	0.7311	6.146
Bottle_11-pCO2=508	8	16	85	8.08	8.06	20.59	489.24	487.51	1861.1	74.30	1956	2022	1.20	2.13	1981	1931	49.6	7	1.000	7.7	0.379	0.00000120	0.500	1.13	8.7	0.886	5.18	57.51	5.876	29.42	528176	9778288	0.42	0.7045	5.656
Bottle_12-pCO2=532	8	16	85	8.06	8.05	21.44	509.44	507.64	1864.0	71.58	1957	2019	1.16	2.05	1981	1934	47.2	7	1.000	9.7	0.351	0.00000114	0.471	1.28	9.7	1.014	5.87	61.04	6.397	31.75	528176	9534995	0.41	0.7408	6.107
Bottle_13-pCO2=723	8	16	85	7.98	7.92	29.09	691.12	688.68	1869.8	53.09	1952	1985	0.86	1.52	1981	1923	57.9	7	1.000	7.9	0.342	0.00000118	0.367	1.21	8.4	0.938	4.08	59.22	6.595	24.58	1022456	8973014	0.31	0.6640	5.848
Bottle_14-pCO2=697	8	16	85	7.94	7.93	28.11	667.90	665.55	1868.0	54.83	1951	1987	0.89	1.57	1981	1920	60.8	7	1.000	7.9	0.342	0.00000095	0.325	1.90	8.5	0.758	3.63	58.28	5.228	21.42	1022456	11137195	0.34	0.7382	5.265
Bottle_15-pCO2=731	8	16	85	7.93	7.91	29.46	699.83	697.36	1872.0	52.55	1954	1986	0.85	1.51	1981	1926	55.0	7	1.000	7.7	0.339	0.00000092	0.317	0.83	9.0	0.784	3.79	62.23	5.432	22.51	1022456	11446580	0.35	0.7129	4.889
Bottle_16-pCO2=215	8	16	85	8.44	8.41	8.90	211.39	210.65	1802.8	161.35	1973	2150	2.61	4.63	1981	1965	16.3	13	0.048	9.5												4296294
Bottle_17-pCO2=349	8	16	85	8.24	8.22	14.27	339.01	337.82	1853.4	106.34	1974	2083	1.72	3.05	1981	1967	14.3	13	0.048	7.8												5678546
Bottle_18-pCO2=506	8	16	85	8.09	8.07	20.48	486.56	484.85	1877.5	76.03	1974	2042	1.23	2.18	1981	1966	15.0	13	0.048	6.4												4483941
Bottle_19-pCO2=587	8	16	85	8.02	8.01	23.68	562.57	560.59	1877.6	65.76	1967	2020	1.06	1.89	1981	1952	28.6	20	0.048	5.8												3040185
Bottle_20-pCO2=707	8	16	85	7.94	7.93	28.41	674.94	672.56	1867.4	54.22	1950	1985	0.88	1.56	1981	1919	62.1	20	0.048	12.5												12784145