Citation:

meps334063

Name: Seawater carbonate chemistry and growth rate during experiments with dinoflagellates, 2007
Published: 2007
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Bottles or small containers/Aquaria (<20 L); Caratium lineatum; Chromista; Growth/Morphology; Heterocapsa triquetra; Laboratory experiment; Laboratory strains; Myzozoa; Pelagos; Phytoplankton; Prorocentrum minimum; Single species

Always quote citation above when using data! You can download the citation in several formats below.

RIS Citation BibTeX Citation Text Citation Facebook Twitter

Abstract:

The effects of dissolved inorganic carbon (DIC) on the growth of 3 red-tide dinoflagellates (Ceratium lineatum, Heterocapsa triquetra and Prorocentrum minimum) were studied at pH 8.0 and at higher pH levels, depending upon the pH tolerance of the individual species. The higher pH levels chosen for experiments were 8.55 for C. lineatum and 9.2 for the other 2 species. At pH 8.0, which approximates the pH found in the open sea, the maximum growth in all species was maintained until the total DIC concentration was reduced below ~0.4 and 0.2 mM for C. lineatum and the other 2 species, respectively. Growth compensation points (concentration of inorganic carbon needed for maintenance of cells) were reached at ~0.18 and 0.05 mM DIC for C. lineatum and the other 2 species, respectively. At higher pH levels, maximum growth rates were lower compared to growth at pH 8, even at very high DIC concentrations, indicating a direct pH effect on growth. Moreover, the concentration of bio-available inorganic carbon (CO2 + HCO3-) required for maintenance as well as the half-saturation constants were increased considerably at high pH compared to pH 8.0. Experiments with pH-drift were carried out at initial concentrations of 2.4 and 1.2 mM DIC to test whether pH or DIC was the main limiting factor at a natural range of DIC. Independent of the initial DIC concentrations, growth rates were similar in both incubations until pH had increased considerably. The results of this study demonstrated that growth of the 3 species was mainly limited by pH, while inorganic carbon limitation played a minor role only at very high pH levels and low initial DIC concentrations.

Keyword(s):

Bottles or small containers/Aquaria (<20 L); Caratium lineatum; Chromista; Growth/Morphology; Heterocapsa triquetra; Laboratory experiment; Laboratory strains; Myzozoa; Pelagos; Phytoplankton; Prorocentrum minimum; Single species

Project(s):

European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)

European Project on Ocean Acidification (EPOCA)

Funding:

Seventh Framework Programme (FP7), grant/award no. 211384 : European Project on Ocean Acidification

Sixth Framework Programme (FP6), grant/award no. 511106 : European network of excellence for Ocean Ecosystems Analysis

Event(s):

Hansen_etal_07/F1 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Hansen, Per Juel
2	Carbonate system computation flag	CSC flag		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
3	Temperature, water	Temp	°C	Hansen, Per Juel
4	Salinity	Sal		Hansen, Per Juel
5	Density, mass density	Density	kg/m³	Hansen, Per Juel	Calculated
6	Radiation, photosynthetically active	PAR	µmol/m²/s	Hansen, Per Juel	PAR sensor LI-1000, LI-COR Inc.
7	Light:Dark cycle	L:D	hh:hh	Hansen, Per Juel	Measured
8	pH	pH		Hansen, Per Juel	pH meter (Sentron, Argus X)	NBS scale
9	pH	pH		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)	Total scale
10	Carbon, inorganic, dissolved	DIC	µmol/kg	Hansen, Per Juel	Infrared gas analyser (ADC, MK3)
11	Alkalinity, total	AT	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
12	Carbon dioxide	CO2	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
13	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
14	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
15	Bicarbonate ion	[HCO3]-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
16	Carbonate ion	[CO3]2-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
17	Aragonite saturation state	Omega Arg		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
18	Calcite saturation state	Omega Cal		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
19	Growth rate	µ	#/day	Hansen, Per Juel	Calculated

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Size:

1349 data points

Download Data

Download dataset as tab-delimited text — use the following character encoding:

View dataset as HTML