PANGAEA_997430 Temperature-dependent changes in dissolved black carbon during heating of marine and terrestrial dissolved organic matter reference materials Knoke, Melina; Dittmar, Thorsten; Niggemann, Jutta; Wagner, Sasha; Förster, Oliver; Leclerc, Ahlyia J.; Ulber, Ina Ida; Günther, Jana; Simon, Heike; Seidel, Michael Temperature-dependent changes in dissolved black carbon during heating of marine and terrestrial dissolved organic matter reference materials 2026-09-29 PANGAEA Dataset https://doi.pangaea.de/10.1594/PANGAEA.997430 Investigator Michael Seidel m.seidel@uol.de Earth Science Event label Type of study Sample code/label Batch Treatment: temperature Replicate Sample source Benzenehexacarboxylic acid Benzenepentacarboxylic acid Benzenetetracarboxylic acid Black carbon, dissolved Black carbon, dissolved Benzenepentacarboxylic acid/Benzenehexacarboxylic acid ratio Benzenepentacarboxylic acid/Benzenetetracarboxylic acid ratio geoscientificInformation BPCA_NEqPIW_heating_exp BPCA_SRNOM_heating_exp Dissolved Black Carbon Dissolved Organic Matter Guaymas Basin hydrothermal mobilization IODP Laboratory experiment NELHA_NEqPIW_2009 North Equatorial Pacific Intermediate Water porewater Reverse osmosis isolation of filtered river water (IHSS protocol) according to Serkiz and Perdue (1990) Solid‑phase extracted (SPE) of filtered seawater according to Green et al. 2014 SRNOM_2012 Suwannee River Natural Organic Matter Benzene polycarboxylic acid method (BPCA); measured with Ultra Performance Liquid Chromatograph (UPLC), Waters Corporation, ACQUITY Calculated according to Stubbins et al. (2015) Calculated 2009-09-24 2025-09-18 Complete 19.7333 53.152839 -156.0667 8.167196 access rights needed CC-BY-4.0: Creative Commons Attribution 4.0 International (License comes into effect after moratorium ends) English PANGAEA Data Publisher for Earth & Environmental Science https://www.pangaea.de/ Data Center Contact Michael Diepenbroek info@pangaea.de

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Bremen Bremen 28359 Germany online 585 data points text/tab-separated-values Knoke, Melina; Böning, Philipp; Dittmar, Thorsten; Friebe, Matthias; Gründken, Eleonore; Günther, Jana; Klaproth, Katrin; Ulber, Ina; Simon, Heike; Seidel, Michael (2025): Molecular composition of dissolved organic matter and biogeochemical parameters in porewater of Guaymas Basin (Gulf of California) deep subsurface sediments [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.974052 Knoke, Melina; Dittmar, Thorsten; Niggemann, Jutta; Wagner, Sasha; Förster, Oliver; Leclerc, Ahlyia J.; Ulber, Ina Ida; Günther, Jana; Simon, Heike; Seidel, Michael (2026): BPCA-derived dissolved black carbon concentrations in porewater and hot-water Soxhlet extracts of Guaymas Basin deep subsurface sediments [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.997437 Knoke, Melina; Dittmar, Thorsten; Niggemann, Jutta; Wagner, Sasha; Förster, Oliver; Leclerc, Ahlyia J.; Ulber, Ina Ida; Günther, Jana; Simon, Heike; Seidel, Michael (2026): Stable carbon isotope composition (δ¹³C) of dissolved black carbon in hot-water Soxhlet extracts [MS1.1] of Guaymas Basin deep subsurface sediments [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.997438 Dittmar, Thorsten (2008): The molecular level determination of black carbon in marine dissolved organic matter. Organic Geochemistry, 39(4), 396-407, https://doi.org/10.1016/j.orggeochem.2008.01.015 Green, Nelson W; Perdue, E Michael; Aiken, George R; Butler, Kenna D; Chen, Hongmei; Dittmar, Thorsten; Niggemann, Jutta; Stubbins, Aron (2014): An intercomparison of three methods for the large-scale isolation of oceanic dissolved organic matter. Marine Chemistry, 161, 14-19, https://doi.org/10.1016/j.marchem.2014.01.012 Kohler, Kevin E; Gill, Shaun M (2006): Coral Point Count with Excel extensions (CPCe): A Visual Basic program for the determination of coral and substrate coverage using random point count methodology. Computers & Geosciences, 32(9), 1259-1269, https://doi.org/10.1016/j.cageo.2005.11.009 Serkiz, Steven M; Perdue, E Michael (1990): Isolation of dissolved organic matter from the suwannee river using reverse osmosis. Water Research, 24(7), 911-916, https://doi.org/10.1016/0043-1354(90)90142-S Stubbins, Aron; Spencer, Robert G M; Mann, Paul James; Holmes, R Max; McClelland, James W; Niggemann, Jutta; Dittmar, Thorsten (2015): Utilizing colored dissolved organic matter to derive dissolved black carbon export by arctic rivers. Frontiers in Earth Science, 3, https://doi.org/10.3389/feart.2015.00063 Wagner, Sasha; Brandes, Jay; Goranov, Aleksandar I; Drake, Travis W; Spencer, Robert G M; Stubbins, Aron (2017): Online quantification and compound‐specific stable isotopic analysis of black carbon in environmental matrices via liquid chromatography‐isotope ratio mass spectrometry. Limnology and Oceanography-Methods, 15(12), 995-1006, https://doi.org/10.1002/lom3.10219

Dissolved black carbon (DBC) is a condensed aromatic fraction of dissolved organic matter (DOM) and an important component of the marine carbon cycle. Hydrothermal systems have been suggested as a potential source of DBC to the deep ocean, but the mobilization and origin of DBC in organic-rich hydrothermal sediments remain poorly constrained. This dataset contains data from heating experiments performed to investigate the temperature-dependent mobilization of dissolved black carbon (DBC) from marine and terrestrial dissolved organic matter (DOM) reference materials under controlled aqueous heating conditions. The experiments were conducted with solid-phase extracted (SPE) marine DOM from North Equatorial Pacific Intermediate Water (NEqPIW), sampled and isolated at the Natural Energy Laboratory of Hawaii Authority (NELHA) as described by Green et al. (2014), and Suwannee River Natural Organic Matter (SRNOM), a terrestrial DOM reference material provided by the International Humic Substances Society (IHSS) and fisrt described by Serkiz & Perdue (1990). The reference materials were dissolved in ultrapure water and afterwards subjected to temperature treatments of 25, 80, 100, and 180 °C. DBC was determined using the benzene polycarboxylic acid (BPCA) method after Dittmar et al. (2008) and Wagner et al., (2017). BPCAs were quantified by ultra-performance liquid chromatography (UPLC), and DBC concentrations were calculated from benzene pentacarboxylic acid (B5CA) and benzene hexacarboxylic acid (B6CA) concentrations according to Stubbins et al. (2015). The dataset includes benzene tetracarboxylic acid (B4CA), B5CA, and B6CA concentrations, BPCA ratios, and calculated DBC concentrations for the different temperature treatments. ** For all details see the full metadata description at "https://doi.pangaea.de/10.1594/PANGAEA.997430"!

https://doi.org/10.1002/lom3.10219 Online quantification and compound‐specific stable isotopic analysis of black carbon in environmental matrices via liquid chromatography‐isotope ratio mass spectrometry https://doi.org/10.1016/0043-1354(90)90142-S Isolation of dissolved organic matter from the suwannee river using reverse osmosis https://doi.org/10.1016/0043-1354(90)90142-S Reverse osmosis isolation of filtered river water (IHSS protocol) according to Serkiz and Perdue (1990) https://doi.org/10.1016/j.cageo.2005.11.009 Coral Point Count with Excel extensions (CPCe): A Visual Basic program for the determination of coral and substrate coverage using random point count methodology https://doi.org/10.1016/j.marchem.2014.01.012 An intercomparison of three methods for the large-scale isolation of oceanic dissolved organic matter https://doi.org/10.1016/j.marchem.2014.01.012 Solid‑phase extracted (SPE) of filtered seawater according to Green et al. 2014 https://doi.org/10.1016/j.orggeochem.2008.01.015 The molecular level determination of black carbon in marine dissolved organic matter https://doi.org/10.3389/feart.2015.00063 Utilizing colored dissolved organic matter to derive dissolved black carbon export by arctic rivers https://doi.pangaea.de/10.1594/PANGAEA.974052 Molecular composition of dissolved organic matter and biogeochemical parameters in porewater of Guaymas Basin (Gulf of California) deep subsurface sediments https://doi.pangaea.de/10.1594/PANGAEA.997437 BPCA-derived dissolved black carbon concentrations in porewater and hot-water Soxhlet extracts of Guaymas Basin deep subsurface sediments https://doi.pangaea.de/10.1594/PANGAEA.997438 Stable carbon isotope composition (δ¹³C) of dissolved black carbon in hot-water Soxhlet extracts [MS1.1] of Guaymas Basin deep subsurface sediments DIF 9.4 2026-09-29 2026-09-29