/* DATA DESCRIPTION:
Citation:	Steiner, Niko; Nilsen, Anne M; Franke, Kiara; Rößler, Leonard; Salvaitis, Dominykas; Rautenberger, Ralf; Bruckner, Christian; Buer, Nikolai; Jueterbock, Alexander; Bartsch, Inka (2026): Growth and biochemistry of Saccharina latissima sporophytes in response to gametophyte priming [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.996568
Abstract:	We examined the effect of gametophyte priming on early life stages of Saccharina latissima (gametophyte growth, sporophyte recruitment, juvenile sporophyte growth) and adult sporophytes harvested after growing for six months in mariculture (size and biomass, biochemical composition) in two experiments. Experiment 1: we primed S. latissima gametophytes (Lofoten 2023; 15°C clonal culture; n = 7) for 3 weeks at 0°C, 10°C, and 20°C and induced gametogenesis at 10°C for 2 weeks, during which we assessed relative gametophyte growth rates (from day 0 – 5), progression of gametogenesis, and sporophyte recruitment. Resulting sporophytes reared at 10°C were subjected to a 14-day temperature gradient treatment spanning 7 temperatures (0, 5, 10, 15, 20, 21.5, 23°C). We measured sporophyte growth (length and relative growth rates) after 7 and 14 days. Experiment 2: primed (0°C, 10°C, 20°C; 3 weeks) gametophytes (Lofoten 2023; 10°C mixed culture; n = 5) were sown on ropes and reared in a mariculture experiment (2 months hatchery, 4 months mariculture). Adult sporophytes were sampled and we measured growth (sporophyte length and width), total biomass (in fresh weight per m rope) and biochemical composition (C, N, mannitol, laminarin, and mineral composition). During the mariculture experiment, seawater temperature and nutrient composition was recorded at different time points. The mariculture field experiment took place at the hatchery of Polaralge AS (Sandhornøy, Norway; December - February 2024) and the deployment site of Lofoten Blue Harvest (Lilje Engla, Lofoten, Norway; 68°16'02.8N 15°06'14.8E; February – June 2024). The laboratory experiments of experiment 1 and the CN-analysis were carried out at the Alfred Wegener Institute in Bremerhaven, Germany; the mannitol analysis at the University of Rostock, Germany; the laminarin analysis at the MPI Bremen, Germany; and the mineral analysis at NIBIO Bodø, Norway.
Keyword(s):	biochemical composition; Field experiment; Kelp; Laboratory experiment; seaweed farming; thermal priming; Transgenerational effect
Related to:	Becker, Stefan; Hehemann, Jan-Hendrik (2018): Laminarin Quantification in Microalgae with Enzymes from Marine Microbes. BIO-PROTOCOL, 8(8), https://doi.org/10.21769/BioProtoc.2666
	Becker, Stefan; Scheffel, André; Polz, Martin F; Hehemann, Jan-Hendrik (2017): Accurate Quantification of Laminarin in Marine Organic Matter with Enzymes from Marine Microbes. Applied and Environmental Microbiology, 83(9), e03389-16, https://doi.org/10.1128/AEM.03389-16
	Karsten, Ulf; Thomas, N D; Weykam, Gabriele; Daniel, Claudia; Kirst, Gunter-Otto (1991): A simple and rapid method for extraction and separation of low molecular weight carbohydrates from macroalgae using high performance liquid chromatography. Plant Physiology and Biochemistry, 29, 373–378
Funding:	The Research Council of Norway (RCN) (URI: http://www.forskningsradet.no/en/Home_page/1177315753906), grant/award no. 334327: KELPRIME - Priming as a kelp engineering technology to enhance yield and secure production under environmental challenges (URI: https://prosjektbanken.forskningsradet.no/en/project/FORISS/334327?Kilde=FORISS&distribution=Ar&chart=bar&calcType=funding&Sprak=no&sortBy=date&sortOrder=desc&resultCount=30&offset=0&Fritekst=kelprime)
Coverage:	MEDIAN LATITUDE: 60.900335 * MEDIAN LONGITUDE: 11.842310 * SOUTH-BOUND LATITUDE: 53.533270 * WEST-BOUND LONGITUDE: 8.580520 * NORTH-BOUND LATITUDE: 68.267400 * EAST-BOUND LONGITUDE: 15.104100
	DATE/TIME START: 2024-01-12T00:00:00 * DATE/TIME END: 2024-06-14T00:00:00
License:	Creative Commons Attribution 4.0 International (CC-BY-4.0) (URI: https://creativecommons.org/licenses/by/4.0/)
Size:	4 datasets
*/
/* TABULAR SUMMARY OF DATASETS LISTED IN THIS COLLECTION: */
Filename	Citation of child dataset
01_Primgro_E1.tab	Steiner, Niko; Nilsen, Anne M; Franke, Kiara; Rößler, Leonard; Salvaitis, Dominykas; Rautenberger, Ralf; Bruckner, Christian; Buer, Nikolai; Jueterbock, Alexander; Bartsch, Inka (2026): Gametogenesis and gametophyte growth of Saccharina latissima in response to gametophyte priming. https://doi.org/10.1594/PANGAEA.996248
02_Primgro_E1-SG.tab	Steiner, Niko; Nilsen, Anne M; Franke, Kiara; Rößler, Leonard; Salvaitis, Dominykas; Rautenberger, Ralf; Bruckner, Christian; Buer, Nikolai; Jueterbock, Alexander; Bartsch, Inka (2026): Growth of juvenile Saccharina latissima sporophytes in response to gametophyte priming. https://doi.org/10.1594/PANGAEA.996440
03_Primgro_E2.tab	Steiner, Niko; Nilsen, Anne M; Franke, Kiara; Rößler, Leonard; Salvaitis, Dominykas; Rautenberger, Ralf; Bruckner, Christian; Buer, Nikolai; Jueterbock, Alexander; Bartsch, Inka (2026): Growth and biochemistry of mariculture grown Saccharina latissima sporophytes in response to gametophyte priming. https://doi.org/10.1594/PANGAEA.996441
04_Primgro_E2-SW.tab	Steiner, Niko; Nilsen, Anne M; Franke, Kiara; Rößler, Leonard; Salvaitis, Dominykas; Rautenberger, Ralf; Bruckner, Christian; Buer, Nikolai; Jueterbock, Alexander; Bartsch, Inka (2026): Seawater temperature and nutrients during a Saccharina latissima gametophyte priming mariculture experiment. https://doi.org/10.1594/PANGAEA.996442
