Steiner Niko steiner1@uni-bremen.de 0000-0003-2559-5891 Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven AWI https://www.awi.de/ https://ror.org/032e6b942 Nilsen Anne M anne.m.nilsen@nord.no Nord University http://www.nord.no/ https://ror.org/030mwrt98 Franke Kiara kiara.franke@uni-rostock.de 0000-0001-6318-8475 University of Rostock https://www.uni-rostock.de/en/ https://ror.org/03zdwsf69 Rößler Leonard lroessler@marum.de MARUM - Center for Marine Environmental Sciences, University Bremen MARUM http://www.marum.de/ https://ror.org/04ers2y35 Salvaitis Dominykas dominykas.salvaitis@nibio.no Norwegian Institute of Bioeconomy Research NIBIO http://www.nibio.no/en https://ror.org/04aah1z61 Rautenberger Ralf 0000-0002-1339-2838 Norwegian Institute of Bioeconomy Research NIBIO http://www.nibio.no/en https://ror.org/04aah1z61 Bruckner Christian christian@polaralge.no Polaralge AS Skjellvik Buer Nikolai nikolai@lofotenblueharvest.com Lofoten Blue Harvest AS Jueterbock Alexander Alexander-Jueterbock@web.de 0000-0002-0659-3172 Nord University http://www.nord.no/ https://ror.org/030mwrt98 Bartsch Inka inka.bartsch@awi.de https://www.awi.de/en/about-us/organisation/staff/single-view/inka-bartsch.html 0000-0001-7609-2149 Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven AWI https://www.awi.de/ https://ror.org/032e6b942 2026 Growth and biochemistry of Saccharina latissima sporophytes in response to gametophyte priming dataset bundled publication https://doi.org/10.1594/PANGAEA.996568 2026-08-17T09:17:09 We examined the effect of gametophyte priming on early life stages of Saccharina latissima (gametophyte growth, sporophyte recruitment, juvenile sporophyte growth) and adult sporophytes harvested after growing for six months in mariculture (size and biomass, biochemical composition) in two experiments. Experiment 1: we primed S. latissima gametophytes (Lofoten 2023; 15°C clonal culture; n = 7) for 3 weeks at 0°C, 10°C, and 20°C and induced gametogenesis at 10°C for 2 weeks, during which we assessed relative gametophyte growth rates (from day 0 – 5), progression of gametogenesis, and sporophyte recruitment. Resulting sporophytes reared at 10°C were subjected to a 14-day temperature gradient treatment spanning 7 temperatures (0, 5, 10, 15, 20, 21.5, 23°C). We measured sporophyte growth (length and relative growth rates) after 7 and 14 days. Experiment 2: primed (0°C, 10°C, 20°C; 3 weeks) gametophytes (Lofoten 2023; 10°C mixed culture; n = 5) were sown on ropes and reared in a mariculture experiment (2 months hatchery, 4 months mariculture). Adult sporophytes were sampled and we measured growth (sporophyte length and width), total biomass (in fresh weight per m rope) and biochemical composition (C, N, mannitol, laminarin, and mineral composition). During the mariculture experiment, seawater temperature and nutrient composition was recorded at different time points. The mariculture field experiment took place at the hatchery of Polaralge AS (Sandhornøy, Norway; December - February 2024) and the deployment site of Lofoten Blue Harvest (Lilje Engla, Lofoten, Norway; 68°16'02.8N 15°06'14.8E; February – June 2024). The laboratory experiments of experiment 1 and the CN-analysis were carried out at the Alfred Wegener Institute in Bremerhaven, Germany; the mannitol analysis at the University of Rostock, Germany; the laminarin analysis at the MPI Bremen, Germany; and the mineral analysis at NIBIO Bodø, Norway. Becker Stefan mail@beckerstefan.com 0000-0002-9619-3542 Hehemann Jan-Hendrik jhheheman@marum.de 0000-0002-8700-2564 2018 Laminarin Quantification in Microalgae with Enzymes from Marine Microbes journal article BIO-PROTOCOL 8(8) https://doi.org/10.21769/BioProtoc.2666 Becker Stefan mail@beckerstefan.com 0000-0002-9619-3542 Scheffel André Polz Martin F Hehemann Jan-Hendrik jhheheman@marum.de 0000-0002-8700-2564 2017 Accurate Quantification of Laminarin in Marine Organic Matter with Enzymes from Marine Microbes journal article Applied and Environmental Microbiology 83(9) https://doi.org/10.1128/AEM.03389-16 e03389-16 Karsten Ulf ulf.karsten@uni-rostock.de 0000-0002-2955-0757 Thomas N D Weykam Gabriele Daniel Claudia claudia.daniel@awi.de Kirst Gunter-Otto 1991 A simple and rapid method for extraction and separation of low molecular weight carbohydrates from macroalgae using high performance liquid chromatography journal article Plant Physiology and Biochemistry 29 373–378 8.58052 15.1041 53.53327 68.2674 11.842310000000001 60.900334999999984 2024-01-12T00:00:00 2024-06-14T00:00:00 The Research Council of Norway RCN http://www.forskningsradet.no/en/Home_page/1177315753906 https://doi.org/10.13039/501100005416 334327 KELPRIME - Priming as a kelp engineering technology to enhance yield and secure production under environmental challenges https://prosjektbanken.forskningsradet.no/en/project/FORISS/334327?Kilde=FORISS&distribution=Ar&chart=bar&calcType=funding&Sprak=no&sortBy=date&sortOrder=desc&resultCount=30&offset=0&Fritekst=kelprime 4 CC-BY-4.0 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ Type of study | Study type | Date | Month | Station ID | Stat ID | Technical replicate | Replicate | Repl | Seawater ammonium | Ammonium | [NH4]+ | Elemental analyzer, EuroVector EA3100-Dual, HEKAtech GmbH | Seawater phosphate | Phosphate | [PO4]3- | Seawater nitrate | Nitrate | [NO3]- | Seawater nitrite | Nitrite | [NO2]- | Temperature, water | Temp | Temperature logger, Onset, HOBO Pendant MX2202 | Day of experiment | DOE | Priming: pre-treatment of gametophytes | Treatment: temperature | T:temp | Gametophytes, total | Gametophytes tot | Calculated from counting: Reverse microscope (CKX41SF, Olympus Corporation) | Gametophytes, female, vegetative | Gametophytes f veg | Gametophytes, female, with oogonia | Gametophytes f oog | Gametophytes, female, with eggs | Gametophytes f eggs | Gametophytes, female, with sporophytes | Gametophytes f sporophyte | Sporophytes, total | Sporophytes tot | Gametophyte area | Gametophyte size | Image analysis NIH ImageJ | Experiment temperature | Counting from image | Sporophyte, length | Sporophyte l | Image analysis, EHDView | Sample ID | Biomass per m rope | Biomass, wet mass | Biom wm | Average sporophyte length of 10 longest sporophytes | Length | l | Average sporophyte width of 10 longest sporophytes | Width | w | of dry weight | Carbon | C | Nitrogen | N | Laminarin | Mannitol | Phosphorus | P | Sulfur | S | Iodine | I | Magnesium | Mg | Calcium | Ca | Sodium | Na | Potassium | K | Arsenic | As | Zinc | Zn | Cadmium | Cd | Copper | Cu | Iron | Fe | Boron | B | Manganese | Mn citable biochemical composition Field experiment Kelp Laboratory experiment seaweed farming thermal priming Transgenerational effect method17225 method19515 method19516 method19517 method6621 method6656 param105163 param129269 param146876 param152481 param156292 param16065 param172977 param179057 param183784 param185520 param188298 param502717 param52 param528059 param528061 param541227 param549729 param563255 param567779 param568473 param568474 param568475 param568476 param568477 param568478 param568479 param568480 param568481 param568482 param568483 param568484 param568485 param568486 param717 param756 param757 param758 param78394 param80167 param93665 param93667 param93668 param93669 param93670 param93671 param93672 param97989 pi118602 pi118603 pi48692 pi55313 pi83713 pi91582 author10394 author109705 author109706 author118601 author118602 author118603 author118604 author118605 author118606 author118607 author20021 author24548 author54500 author55313 author58368 author70082 author74284 author83713 author91582 award5177 funder20398 geocode1599 geocode1600 geocode1601 inst21195 inst21448 inst22906 inst22907 inst22908 inst277 inst32 journal1291 journal12970 journal17593 license21 ref131795 ref140843 ref140844 term38841 term38842 term52406 term72869 term78418 term78419 term78420