Steiner Niko steiner1@uni-bremen.de 0000-0003-2559-5891 Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven AWI https://www.awi.de/ https://ror.org/032e6b942 Nilsen Anne M anne.m.nilsen@nord.no Nord University http://www.nord.no/ https://ror.org/030mwrt98 Gauci Clément clement.gauci@nord.no Nord University http://www.nord.no/ https://ror.org/030mwrt98 Jueterbock Alexander Alexander-Jueterbock@web.de 0000-0002-0659-3172 Nord University http://www.nord.no/ https://ror.org/030mwrt98 Bartsch Inka inka.bartsch@awi.de https://www.awi.de/en/about-us/organisation/staff/single-view/inka-bartsch.html 0000-0001-7609-2149 Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven AWI https://www.awi.de/ https://ror.org/032e6b942 2026 Heat stress response of Saccharina latissima sporophytes of different age classes to gametophyte and sporophyte priming dataset bundled publication https://doi.pangaea.de/10.1594/PANGAEA.996547 2026-10-22T08:53:21 We assessed the heat tolerance of microscopic, juvenile and adult sporophytes under heat stress and in response to gametophyte or sporophyte priming in five experiments. Experiment 1: we primed Saccharina latissima gametophytes (Lofoten 2023; 15°C clonal culture; n = 7) for 3 weeks at 0°C, 10°C, and 20°C and induced gametogenesis at 10°C for 2 weeks. Resulting microscopic sporophytes were subjected to a 14-day heat stress treatment at 20°C, 21.5°C, and 23°C, where we counted the number of alive and dead sporophytes and calculated the survival rates. Experiment 2: sporophytes were cultivated after gametogenesis for an additional 4 weeks before we subjected them to a 14-day simulated heat wave (13°C for 3 days, 16°C for 2 days, 18°C for 2 days, 20°C, 21°C and 22°C for 1 day each, 23°C and 24°C for 4 days each, and recovery at 20°C for 7 days), during which we assessed the photosynthetic performance by measuring quantum yield (Fv/Fm). Sporophytes that survived the heat wave were cultivated at 15°C for 64 days to recover, after which we measured their size (length and blade area). Experiment 3: primed (0°C, 10°C, 20°C; 3 weeks) gametophytes (Lofoten 2023; 10°C mixed culture; n = 5) were sown on ropes and reared in a mariculture experiment (2 months hatchery, 4 months mariculture). Adult sporophytes were sampled and exposed to the same 14-day heat stress treatment at 20°C, 21.5°C, 23°C with a 10°C control group. We measured growth (length and blade area), Fv/Fm, and the percentage of healthy area (as % blade area with Fv/Fm > 0.6). Experiment 4: sporophytes from the 0°C gametophyte priming treatment of the same mariculture experiment recovered at 10°C for 24 days. Meristematic discs were exposed to a 1-day sporophyte priming trigger (T). A control group (C) remained at 10°C. After 3 days recovery at 10°C both groups were subjected to a 7-day heat stress treatment at 20°C, 21.5°C, 23°C, and 25°C, where we measured growth (disc area) and Fv/Fm. Experiment 5: gametogenesis of gametophytes (Spitsbergen 2011, 2015; 5°C clonal culture, n = 5) was induced at 5°C and sporophytes reared for 6 months. Sporophytes were distributed to three treatment groups. A 17-day heat wave (from 5°C to 20°C and back) followed by a 1-day priming trigger at 21.5°C (HWT); 17 days at 10°C and a 1-day priming trigger (T); a control remaining at 10°C throughout (C). All groups were subjected to a 7-day heat stress treatment at 20°C, 21.5°C, 23°C, and 25°C, where we measured Fv/Fm, percentage of healthy area and survival. All laboratory experiments were carried out at the Alfred Wegener Institute in Bremerhaven, from January to July 2024. The mariculture experiment took place at the hatchery of Polaralge AS (Sandhornøy, Norway; December - February 2024) and the deployment site of Lofoten Blue Harvest (Lilje Engla, Lofoten, Norway; 68°16'02.8N 15°06'14.8E; February – June 2024). Steiner Niko steiner1@uni-bremen.de 0000-0003-2559-5891 Nilsen Anne M anne.m.nilsen@nord.no Gauci Clément clement.gauci@nord.no Jueterbock Alexander Alexander-Jueterbock@web.de 0000-0002-0659-3172 Bartsch Inka inka.bartsch@awi.de https://www.awi.de/en/about-us/organisation/staff/single-view/inka-bartsch.html 0000-0001-7609-2149 2026 Differential response of gametophyte and sporophyte priming to heat stress of Saccharina latissima sporophytes across different age classes journal article Marine Ecology Progress Series 794 https://doi.org/10.3354/meps15253 1-24 8.580520000000005 8.580520000000005 53.53326999999997 53.53326999999997 8.580520000000005 53.53326999999997 2024-01-26T00:00:00 2024-07-26T00:00:00 The Research Council of Norway RCN http://www.forskningsradet.no/en/Home_page/1177315753906 https://doi.org/10.13039/501100005416 334327 KELPRIME - Priming as a kelp engineering technology to enhance yield and secure production under environmental challenges https://prosjektbanken.forskningsradet.no/en/project/FORISS/334327?Kilde=FORISS&distribution=Ar&chart=bar&calcType=funding&Sprak=no&sortBy=date&sortOrder=desc&resultCount=30&offset=0&Fritekst=kelprime 6 CC-BY-4.0 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ Type of study | Study type | Date | Day of experiment | DOE | Gametophyte priming temperature | Treatment: temperature | T:temp | Replicate | Repl | Sample ID | Heatwave simulation T | Maximum quantum yield of photosystem II | Fv/Fm | Pulse-Amplitude-Modulated (PAM) fluorometer, WALZ GmbH, Germany, M-Series | Sporophyte blade length | Blade length | Blade l | Image analysis NIH ImageJ | Sporophyte blade area | Blade area | Sporophyte priming temperature | Treatment | Treat | Sporophyte disc number | Disc area | Area | Heat stress temperature | Sporophytes, live | Sporophytes live | Calculated from counting: Reverse microscope (CKX41SF, Olympus Corporation) | Sporophytes, dead | Sporophytes dead | Calculated in relation to Day 0 | Survival | Heatwave simulation | Sporophyte blade area, total | Sporophyte blade area tot | healthy = Fv/Fm > 0.6 | Sporophyte blade area, healthy | Sporophyte blade area healthy | Number of live sporophytes | Surv | Visual observation | Sporophyte survival heat stress heatwave Kelp Laboratory experiment thermal priming thermal tolerance method18289 method19515 method530 method6656 param105163 param129269 param137029 param152481 param16065 param169871 param174816 param185520 param523295 param524576 param579000 param579001 param579002 param579003 param579004 param78394 param86991 param96278 pi91582 author118601 author118686 author20021 author55313 author91582 award5177 funder20398 geocode1599 geocode1600 geocode1601 inst22906 inst32 journal10811 license21 ref142020 term38841 term52406 term52916 term53689 term60805 term78418