PANGAEA_996429 Chemical analysis of explosive compounds in common dab collected during RV Heincke expedition HE635 from munition dumping areas west of Sylt (NNW03L) and south-west of Heligoland (North Sea) Brüning, Romy; Bünning, Lillian Tabea Hannah; Scheer, Lars Christian; Strehse, Jennifer Susanne; Lindemeyer, Jacqueline; Gundlach, Maren; Schuster, Romina Marietta; Marx, Ute; Brenner, Matthias; Maser, Edmund Chemical analysis of explosive compounds in common dab collected during RV Heincke expedition HE635 from munition dumping areas west of Sylt (NNW03L) and south-west of Heligoland (North Sea) 2026-08-06 PANGAEA Dataset https://doi.pangaea.de/10.1594/PANGAEA.996429 Investigator Romina Marietta Schuster romina.schuster@awi.de Investigator Ute Marx Ute.Marx@awi.de Investigator Lillian Tabea Hannah Bünning buenning@toxi.uni-kiel.de Investigator Romy Brüning bruening@toxi.uni-kiel.de Earth Science Event label Comment Sample ID DATE/TIME LATITUDE LONGITUDE Location ELEVATION Limanda limanda, total length Limanda limanda, wet mass Limanda limanda, sex Limanda limanda, muscle, 2,4,6-trinitrotoluene, per dry mass Validation flag/comment Limanda limanda, muscle, 4-amino-2,6-dinitrotoluene, per dry mass Limanda limanda, muscle, 2-amino-4,6-dinitrotoluene, per dry mass Limanda limanda, muscle, 1,3-dinitrobenzene, per dry mass Limanda limanda, muscle, 2,4-dinitrotoluene, per dry mass Limanda limanda, muscle, hexahydro-1,3,5-trinitro-1,3,5-triazine, per dry mass Limanda limanda, muscle, octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine, per dry mass Limanda limanda, bile, 2,4,6-trinitrotoluene Limanda limanda, bile, 4-amino-2,6-dinitrolouene Limanda limanda, bile, 2-amino-4,6-dinitrolouene Limanda limanda, bile, 1,3-dinitrobenzene Limanda limanda, bile, 2,4-dinitrotoluene Limanda limanda, bile, hexahydro-1,3,5-trinitro-1,3,5-triazine Limanda limanda, bile, octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine Limanda limanda, liver, 2,4,6-trinitrotoluene, per wet mass Limanda limanda, liver, 4-amino-2,6-dinitrotoluene, per wet mass Limanda limanda, liver, 2-amino-4,6-dinitrotoluene, per wet mass Limanda limanda, liver, 1,3-dinitrobenzene, per wet mass Limanda limanda, liver, 2,4-dinitrotoluene, per wet mass Limanda limanda, liver, hexahydro-1,3,5-trinitro-1,3,5-triazine, per wet mass Limanda limanda, liver, octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine, per wet mass Limanda limanda, blood, 2,4,6-trinitrotoluene, per wet mass Limanda limanda, blood, 4-amino-2,6-dinitrotoluene, per wet mass Limanda limanda, blood, 2-amino-4,6-dinitrotoluene, per wet mass Limanda limanda, blood, 1,3-dinitrobenzene, per wet mass Limanda limanda, blood, 2,4-dinitrotoluene, per wet mass Limanda limanda, blood, octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine, per wet mass Limanda limanda, blood, hexahydro-1,3,5-trinitro-1,3,5-triazine, per wet mass Limanda limanda, urine, 2,4,6-trinitrotoluene Limanda limanda, urine, 4-amino-2,6-dinitrotoluene Limanda limanda, urine, 2-amino-4,6-dinitrotoluene Limanda limanda, urine, 1,3-dinitrobenzene Limanda limanda, urine, 2,4-dinitrotoluene Limanda limanda, urine, hexahydro-1,3,5-trinitro-1,3,5-triazine Limanda limanda, urine, octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine Limanda limanda, gills, 2,4,6-trinitrotoluene, per dry mass Limanda limanda, gills, 4-amino-2,6-dinitrotoluene, per dry mass Limanda limanda, gills, 2-amino-4,6-dinitrotoluene, per dry mass Limanda limanda, gills, 1,3-dinitrobenzene, per dry mass Limanda limanda, gills, 2,4-dinitrotoluene, per dry mass Limanda limanda, gills, octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine, per dry mass Limanda limanda, gills, hexahydro-1,3,5-trinitro-1,3,5-triazine, per dry mass geoscientificInformation Bottom trawl HE635 HE635_48-1 HE635_89-1 CTD Measuring board Precision scale, Sartorius, R160P Visual inspection Gas chromatography, coupled with tandem mass spectrometer (GC-MS/MS), Thermo Scientific, TSQ8000 Evo Liquid chromatography tandem mass spectrometry (LC-MS/MS), AB SCIEX, QTRAP 5500 Heincke 2024-03-17 2024-03-20 Complete 54.15 55.05 7.92 8.01 -47.0 m a.s.l. (ELEVATION) -15.0 m a.s.l. (ELEVATION) CONMAR DAM sustainMare - CONMAR: CONcepts for conventional MArine Munition Remediation in the German North and Baltic Sea sustainMare Research Mission of the German Marine Research Alliance (DAM): Protection and sustainable use of marine areas unrestricted CC-BY-4.0: Creative Commons Attribution 4.0 International English PANGAEA Data Publisher for Earth & Environmental Science https://www.pangaea.de/ Data Center Contact Michael Diepenbroek info@pangaea.de

Leobener Str.

Bremen Bremen 28359 Germany online 4398 data points text/tab-separated-values Schuster, Romina Marietta; Binder, Franziska Isabell; Bünning, Lillian Tabea Hannah; Brüning, Romy; Grassel, Philipp; Wichert, Uwe; Maser, Edmund; Brenner, Matthias (submitted): Decades after the wars – flatfish common dab (Limanda limanda) caught around military wrecks loaded with munitions in the North Sea show severe health impairments. Environmental Pollution Bünning, Tobias Hartwig; Strehse, Jennifer; Hollmann, Ann; Bötticher, Tom; Maser, Edmund (2021): A Toolbox for the Determination of Nitroaromatic Explosives in Marine Water, Sediment, and Biota Samples on Femtogram Levels by GC-MS/MS. Toxics, 9(3), 60, https://doi.org/10.3390/toxics9030060 Brüning, Romy (2026): Quantification and detection limits of explosive compounds in the study. https://download.pangaea.de/reference/140782/attachments/LOD_LOQ_Fish.pdf

This dataset documents field investigations regarding the release of legacy World War I and II munition explosive compounds into marine biota, focusing on munition dumping areas west of the island of Sylt and south west of the island of Heligoland, North Sea. Flatfish Limanda limanda (common dab) was chosen as a sentinel species. Sampling was conducted during the HE635 cruise in March 2024 with the research vessel Heincke of the Alfred Wegener Institute (AWI). Fish were caught using bottom trawls deployed close to the borders of the dumping areas marked on the sea charts. Before dissection, captured fish were transferred to seawater tanks, and only live fish were taken for analysis. Each individual was measured, weighed, and after killing the fish livers were assessed macroscopically. In addition, biometric factors were measured to determine condition factors as indicators of general health. Tissue samples of gills, liver, and muscle, as well as blood, bile, and urine, were taken, immediately frozen in liquid nitrogen, and stored at -20°C before chemical analysis. Sample processing in the laboratory followed established protocols (Bünning et al. 2021). In brief, all samples were homogenized and then extracted using solid phase extraction (SPE). Bile, urine, and blood samples were treated with β-glucuronidase (Helix pomatia), followed by a liquid-liquid extraction step for blood and bile samples before SPE. All samples were analyzed by gas chromatography coupled with triple quadrupole mass spectrometry (GC-MS/MS) for the explosive compound 2,4,6-trinitrotoluene (TNT), its metabolites 2-amino-4,6-dinitrotoluene (2-ADNT) and 4-amino-2,6-dinitrotoluene (4-ADNT), and the explosive by-products 2,4-dinitrotoluene (2,4-DNT) and 1,3-dinitrobenzene (1,3-DNB). Additionally, liquid chromatography coupled with triple quadrupole mass spectrometry (LC-MS/MS) was used to quantify the nitramine explosives hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX). The dataset includes measured compound concentrations and associated sample metadata. ** For all details see the full metadata description at "https://doi.pangaea.de/10.1594/PANGAEA.996429"! ** NNW03L: dumping site code from the Federal and State Monitoring Programme (BLMP)

http://en.wikipedia.org/wiki/Elevation ELEVATION http://en.wikipedia.org/wiki/ISO_6709 LATITUDE http://en.wikipedia.org/wiki/ISO_6709 LONGITUDE http://en.wikipedia.org/wiki/ISO_8601 DATE/TIME http://www.marinespecies.org/aphia.php?p=taxdetails&id=127139 Limanda limanda, total length https://conmar-munition.eu/ CONMAR https://doi.org/10.17815/jlsrf-3-164 Heincke https://doi.org/10.3390/toxics9030060 A Toolbox for the Determination of Nitroaromatic Explosives in Marine Water, Sediment, and Biota Samples on Femtogram Levels by GC-MS/MS https://en.wikipedia.org/wiki/Common_dab Limanda limanda, wet mass https://www.sustainmare.de/index.php.en sustainMare DIF 9.4 2026-08-06 2026-09-04