10.1594/PANGAEA.996429Brüning, RomyRomyBrüning0009-0000-5589-4249Kiel UniversityBünning, Lillian Tabea HannahLillian Tabea HannahBünning0000-0002-2147-6804Kiel UniversityScheer, Lars ChristianLars ChristianScheerKiel UniversityStrehse, Jennifer SusanneJennifer SusanneStrehseKiel UniversityLindemeyer, JacquelineJacquelineLindemeyerKiel UniversityGundlach, MarenMarenGundlachKiel UniversitySchuster, Romina MariettaRomina MariettaSchusterAlfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, BremerhavenMarx, UteUteMarxAlfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, BremerhavenBrenner, MatthiasMatthiasBrennerAlfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, BremerhavenMaser, EdmundEdmundMaserKiel UniversityPANGAEA2026Event labelCommentSample IDDATE/TIMELATITUDELONGITUDELocationELEVATIONLimanda limanda, total lengthLimanda limanda, wet massLimanda limanda, sexLimanda limanda, muscle, 2,4,6-trinitrotoluene, per dry massValidation flag/commentLimanda limanda, muscle, 4-amino-2,6-dinitrotoluene, per dry massLimanda limanda, muscle, 2-amino-4,6-dinitrotoluene, per dry massLimanda limanda, muscle, 1,3-dinitrobenzene, per dry massLimanda limanda, muscle, 2,4-dinitrotoluene, per dry massLimanda limanda, muscle, hexahydro-1,3,5-trinitro-1,3,5-triazine, per dry massLimanda limanda, muscle, octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine, per dry massLimanda limanda, bile, 2,4,6-trinitrotolueneLimanda limanda, bile, 4-amino-2,6-dinitroloueneLimanda limanda, bile, 2-amino-4,6-dinitroloueneLimanda limanda, bile, 1,3-dinitrobenzeneLimanda limanda, bile, 2,4-dinitrotolueneLimanda limanda, bile, hexahydro-1,3,5-trinitro-1,3,5-triazineLimanda limanda, bile, octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocineLimanda limanda, liver, 2,4,6-trinitrotoluene, per wet massLimanda limanda, liver, 4-amino-2,6-dinitrotoluene, per wet massLimanda limanda, liver, 2-amino-4,6-dinitrotoluene, per wet massLimanda limanda, liver, 1,3-dinitrobenzene, per wet massLimanda limanda, liver, 2,4-dinitrotoluene, per wet massLimanda limanda, liver, hexahydro-1,3,5-trinitro-1,3,5-triazine, per wet massLimanda limanda, liver, octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine, per wet massLimanda limanda, blood, 2,4,6-trinitrotoluene, per wet massLimanda limanda, blood, 4-amino-2,6-dinitrotoluene, per wet massLimanda limanda, blood, 2-amino-4,6-dinitrotoluene, per wet massLimanda limanda, blood, 1,3-dinitrobenzene, per wet massLimanda limanda, blood, 2,4-dinitrotoluene, per wet massLimanda limanda, blood, octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine, per wet massLimanda limanda, blood, hexahydro-1,3,5-trinitro-1,3,5-triazine, per wet massLimanda limanda, urine, 2,4,6-trinitrotolueneLimanda limanda, urine, 4-amino-2,6-dinitrotolueneLimanda limanda, urine, 2-amino-4,6-dinitrotolueneLimanda limanda, urine, 1,3-dinitrobenzeneLimanda limanda, urine, 2,4-dinitrotolueneLimanda limanda, urine, hexahydro-1,3,5-trinitro-1,3,5-triazineLimanda limanda, urine, octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocineLimanda limanda, gills, 2,4,6-trinitrotoluene, per dry massLimanda limanda, gills, 4-amino-2,6-dinitrotoluene, per dry massLimanda limanda, gills, 2-amino-4,6-dinitrotoluene, per dry massLimanda limanda, gills, 1,3-dinitrobenzene, per dry massLimanda limanda, gills, 2,4-dinitrotoluene, per dry massLimanda limanda, gills, octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine, per dry massLimanda limanda, gills, hexahydro-1,3,5-trinitro-1,3,5-triazine, per dry massBottom trawlCTDMeasuring boardPrecision scale, Sartorius, R160PVisual inspectionGas chromatography, coupled with tandem mass spectrometer (GC-MS/MS), Thermo Scientific, TSQ8000 EvoLiquid chromatography tandem mass spectrometry (LC-MS/MS), AB SCIEX, QTRAP 5500HE635HeinckeDAM sustainMare - CONMAR: CONcepts for conventional MArine Munition Remediation in the German North and Baltic Sea (CONMAR)Research Mission of the German Marine Research Alliance (DAM): Protection and sustainable use of marine areas (sustainMare)2024-03-17T07:09:45/2024-03-20T07:11:05Dataset10.3390/toxics90300604398 data pointstext/tab-separated-valuesCreative Commons Attribution 4.0 InternationalThis dataset documents field investigations regarding the release of legacy World War I and II munition explosive compounds into marine biota, focusing on munition dumping areas west of the island of Sylt and south west of the island of Heligoland, North Sea. Flatfish Limanda limanda (common dab) was chosen as a sentinel species. Sampling was conducted during the HE635 cruise in March 2024 with the research vessel Heincke of the Alfred Wegener Institute (AWI). Fish were caught using bottom trawls deployed close to the borders of the dumping areas marked on the sea charts. Before dissection, captured fish were transferred to seawater tanks, and only live fish were taken for analysis. Each individual was measured, weighed, and after killing the fish livers were assessed macroscopically. In addition, biometric factors were measured to determine condition factors as indicators of general health. Tissue samples of gills, liver, and muscle, as well as blood, bile, and urine, were taken, immediately frozen in liquid nitrogen, and stored at -20°C before chemical analysis. Sample processing in the laboratory followed established protocols (Bünning et al. 2021). In brief, all samples were homogenized and then extracted using solid phase extraction (SPE). Bile, urine, and blood samples were treated with β-glucuronidase (Helix pomatia), followed by a liquid-liquid extraction step for blood and bile samples before SPE. All samples were analyzed by gas chromatography coupled with triple quadrupole mass spectrometry (GC-MS/MS) for the explosive compound 2,4,6-trinitrotoluene (TNT), its metabolites 2-amino-4,6-dinitrotoluene (2-ADNT) and 4-amino-2,6-dinitrotoluene (4-ADNT), and the explosive by-products 2,4-dinitrotoluene (2,4-DNT) and 1,3-dinitrobenzene (1,3-DNB). Additionally, liquid chromatography coupled with triple quadrupole mass spectrometry (LC-MS/MS) was used to quantify the nitramine explosives hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX). The dataset includes measured compound concentrations and associated sample metadata.NNW03L: dumping site code from the Federal and State Monitoring Programme (BLMP)7.928.0154.1555.05North SeaFederal Ministry of Education and Researchhttps://doi.org/10.13039/50110000234703F0912BDAM sustainMare - CONMAR, AWIFederal Ministry of Education and Researchhttps://doi.org/10.13039/50110000234703F0912HDAM sustainMare - CONMAR, Kiel University