Rump Marco marco.rump@awi.de 0009-0004-2329-4889 Alfred Wegener Institute - Biological Institute Helgoland AWI Helgoland (BAH) https://www.awi.de/en/about-us/sites/helgoland.html https://ror.org/032e6b942 Lannig Gisela Gisela.Lannig@awi.de https://www.awi.de/en/about-us/organisation/staff/single-view/gisela-lannig.html 0000-0002-9210-256X Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven AWI https://www.awi.de/ https://ror.org/032e6b942 Bock Christian Christian.Bock@awi.de https://www.awi.de/en/about-us/organisation/staff/single-view/christian-bock.html 0000-0003-0052-3090 Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven AWI https://www.awi.de/ https://ror.org/032e6b942 Benthien Albert albert.benthien@awi.de https://www.awi.de/en/about-us/organisation/staff/single-view/albert-benthien.html 0000-0002-9159-3109 Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven AWI https://www.awi.de/ https://ror.org/032e6b942 Pogoda Bernadette bernadette.pogoda@awi.de 0000-0003-3997-426X Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven AWI https://www.awi.de/ https://ror.org/032e6b942 Antoni Dominik dominik.antoni@outlook.com Alfred Wegener Institute - Biological Institute Helgoland AWI Helgoland (BAH) https://www.awi.de/en/about-us/sites/helgoland.html https://ror.org/032e6b942 Gerdts Gunnar Gunnar.Gerdts@awi.de https://www.awi.de/en/about-us/organisation/staff/single-view/gunnar-gerdts.html 0000-0003-0872-3927 Alfred Wegener Institute - Biological Institute Helgoland AWI Helgoland (BAH) https://www.awi.de/en/about-us/sites/helgoland.html https://ror.org/032e6b942 2026 Seawater carbonate chemistry, mortality, tissue trace metal and metabolomic data from a chronic ocean alkalinity enhancement exposure experiment with the European flat oyster Ostrea edulis dataset bundled publication https://doi.pangaea.de/10.1594/PANGAEA.996264 2026-07-29T14:05:54 Adult European flat oysters (Ostrea edulis) were exposed in indoor mesocosms to two contrasting approaches for ocean alkalinity enhancement (OAE): dissolved alkalinity addition using NaOH and CaCl2, and olivine-based coastal enhanced silicate weathering, each targeting a total alkalinity increase of +250 or +500 µmol/kg over unamended seawater. An alkalinization phase (17 April to 19 June 2024) established the target total alkalinity in each treatment tank before oysters were introduced for a subsequent chronic exposure phase (8 May to 10 July 2024). Seawater pH and total alkalinity, salinity, temperature, dissolved oxygen, chlorophyll a, suspended particulate matter and ammonia were monitored throughout both phases, alongside oyster mortality. Full carbonate system parameters (CO2, HCO3-, CO32-, dissolved inorganic carbon, pCO2 and the aragonite and calcite saturation states) were calculated from the measured pH, total alkalinity, salinity and temperature using the seacarb package in R. At the end of the chronic exposure, oyster gill, mantle and hepatopancreas tissue was sampled for trace metal accumulation (nickel, chromium and cobalt) by ICP-OES, gill and mantle tissue for untargeted polar metabolite profiling by ¹H-NMR spectroscopy, and labial palps tissue for glycogen quantification. A separate short-term feeding experiment (12 to 21 November 2024) tested whether the shellfish diet used to feed the oysters (Reed Mariculture SD1800) contributes to the total alkalinity increase observed in the treatment tanks, independent of the OAE treatments. This submission bundles nine related data tables from this study; an individual, table-specific abstract accompanies each file in the file description. Rump Marco marco.rump@awi.de 0009-0004-2329-4889 et al. submitted Ecotoxicological impacts of Ocean Alkalinity Enhancement on the European flat oyster Ostrea edulis: Insights from tissue metabolomics and metal analysis journal article 7.888943999999997 7.888943999999997 54.18032700000003 54.18032700000003 7.888943999999997 54.18032700000003 2024-04-17T00:00:00 2025-03-12T00:00:00 Bundesamt für Naturschutz BfN https://doi.org/10.13039/501100010415 3522830300 DEFINE2 https://www.awi.de/en/science/biosciences/shelf-sea-system-ecology/main-research-focus/european-oyster/translate-to-english-define.html ChronicOystersOAE_2024 54.180327 7.888944 Helgoland, Germany 2024-04-17T00:00:00 54.180327 7.888944 Helgoland, Germany 2025-03-12T00:00:00 Mesocosm experiment MESO http://invabio.wdc-mare.org/mesocubi.jpg 9 CC-BY-4.0 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ Type of study | Study type | Sampling date/time, experiment | Date/time sampling exp | Experiment day | Exp day | Treatment | Treat | Replicates | Repl | pH | Alkalinity, total | AT | Individual code | measurement | Run | Tissue, wet mass | Tis WM | of tissue, wet mass | Glycogen | GLY | Tissues | Sample ID | Individual oyster ID | Individual ID | Ind ID | Sampling date | Tissue, dry mass | Tis DM | Empty | Vial, mass | Vial m | Plus digested sample weight | Digested sample solution | Sample, wet mass | Samp wet m | Nickel | Ni | Chromium | Cr | Cobalt | Co | per tissue dry mass | Adenylates, total | Adenylates | Acetate | Alanine | Ala | Aspartate | Betaine | Choline | Fumarate | Glutamate | Glycine | Gly | Homarine | Hypotaurine | Isoleucine | Ile | Lactate | Leucine | Leu | Methionine | Met | N-Acetylcysteine | CysNAc | O-Acetylcholine | O-Phosphocholine | Succinate | Taurine | Tau | Threonine | Thr | Trimethylamine | TMA | Trimethylamine N-oxide | TMAO | Tyrosine | Tyr | Valine | Val | sn-Glycero-3-phosphocholine | beta-Alanine | b-ala | Incubation type | Inc type | Ostrea edulis, alive | O. edulis alive | Ostrea edulis, dead | O. edulis d | Cumulative | Relative cumulative | Mortality | Temperature, water | Temp | Oxygen | O2 | O | Conductivity | Cond | Salinity | Sal | Chlorophyll a, total | TChl a | Suspended particulate matter | SPM | Ammonia | NH3 | pH, NBS scale | pH NBS | Hydrogen ion, activity coefficient | H+ activity coef | pH, total scale | pHT | Carbon dioxide, dissolved | CO2 diss | Fugacity of carbon dioxide in seawater | fCO2w | Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) | pCO2water_SST_wet | Bicarbonate ion | [HCO3]- | Carbonate ion | [CO3]2- | Carbon, inorganic, dissolved | DIC | Aragonite saturation state | Omega Arg | Calcite saturation state | Omega Cal ecotoxicology ocean alkalinity enhancement Ostrea edulis param102658 param117055 param123837 param127978 param131492 param14025 param16065 param171793 param181964 param185520 param186894 param186896 param186898 param186899 param186900 param186902 param186906 param186908 param186909 param186911 param186912 param186913 param186914 param186920 param186923 param186924 param186925 param186926 param186928 param186929 param186932 param1882 param189884 param20788 param21470 param49313 param507333 param509331 param514586 param516121 param519823 param521056 param521296 param521298 param524036 param524039 param524047 param528880 param534368 param534369 param54293 param54294 param54371 param567650 param567651 param567652 param567653 param567654 param567655 param567656 param716 param717 param764 param79665 param79666 param79669 param80114 param8137 param82364 param82365 param82870 param87073 param94778 param96278 pi118574 author118574 author118575 author21598 author27272 author35983 author38738 author38741 author61559 award5172 event3421985 funder1611 geocode1599 geocode1600 geocode1601 inst1842 inst32 license21 method11277 ref140834 term51393 term73463 term73904 term77752