Marco Rump
Rump
Marco
Alfred Wegener Institute - Biological Institute Helgoland
AWI Helgoland (BAH)
Gisela Lannig
Lannig
Gisela
Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven
AWI
Christian Bock
Bock
Christian
Albert Benthien
Benthien
Albert
Bernadette Pogoda
Pogoda
Bernadette
Dominik Antoni
Antoni
Dominik
Gunnar Gerdts
Gerdts
Gunnar
Tissue-accumulated nickel, chromium and cobalt concentrations in Ostrea edulis measured by ICP-OES at the end of a chronic alkalinity-enhancement exposure experiment
PANGAEA
Data Publisher for Earth & Environmental Science
dataset
This dataset contains nickel, chromium and cobalt concentrations measured in gill, mantle and hepatopancreas tissue of individual European flat oysters (Ostrea edulis) sampled on day 63 (9 July 2024) at the end of the chronic alkalinity-enhancement exposure experiment. A total of 19 individual oysters were sampled (5 control, 5 olivine 250, 3 olivine 500, 3 dissolved 250 and 3 dissolved 500); not all three tissues could be obtained from every individual due to tissue availability. Ten measurements of the certified reference material ERM-CE278k (mussel tissue) are included across the three tissue batches for quality control. Samples for metal analysis were freeze-dried for 24 h, and dry weight was recorded prior to extraction. Extraction vials were pre-cleaned (p.a. quality) with concentrated nitric acid (65 %, single-distilled) and dried for 48 h. Approximately 2 mL of nitric acid was added to the dried tissues, followed by thermic digestion using a microwave digestion system (5 min heating at 400 W; 5 min heating to 100 °C at 800 W; 20 min heating to 175 °C and 20 min held, 1600 W). The digested solutions were transferred to clean vials and diluted to 25 mL; vials were weighed before and after filling to determine the precise sample weight. Metal concentrations of Ni, Cr and Co were measured on 12 March 2025 at the AWI ICP Facility on a Thermo Scientific iCAP 7000 Series Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES). Calibration was performed with an in-house standard composed of the respective individual element standards (ICP-Standards ROTI® Star, Carl Roth). The standard deviation of the control standard was 0.1 µg/l for Ni, 0.3 µg/l for Cr and 0.2 µg/l for Co. External reproducibility (RSD) was 0.5 % for Ni, 1.2 % for Cr and 0.7 % for Co. Limits of detection and quantification were 0.13 and 0.7 µg/l for Ni; 0.26 and 1.32 µg/l for Cr; and 0.10 and 0.47 µg/l for Co. Analytical accuracy was verified with the certified reference material ERM-CE278k.
et al.
Ecotoxicological impacts of Ocean Alkalinity Enhancement on the European flat oyster Ostrea edulis: Insights from tissue metabolomics and metal analysis
submitted
journal article
Carbonate chemistry parameters calculated from measured pH, total alkalinity, salinity and temperature in the alkalization mesocosms of a chronic Ostrea edulis alkalinity-enhancement exposure experiment
Carbonate chemistry parameters calculated from measured pH, total alkalinity, salinity and temperature in the exposure mesocosms of a chronic Ostrea edulis alkalinity-enhancement exposure experiment
Concentrations of 27 polar metabolites in gill and mantle tissue of Ostrea edulis measured by ¹H-NMR spectroscopy during a chronic alkalinity-enhancement exposure experiment
Glycogen concentration in the labial palps of Ostrea edulis during a chronic alkalinity-enhancement exposure experiment
Oyster mortality and physicochemical parameters in the exposure mesocosms of a chronic Ostrea edulis alkalinity-enhancement exposure experiment
Total alkalinity and pH during a short-term feeding experiment testing the contribution of shellfish diet (Reed Mariculture SD1800) to seawater alkalinity
Total alkalinity and pH in the alkalization mesocosms of a chronic Ostrea edulis alkalinity-enhancement exposure experiment
Total alkalinity and pH in the exposure mesocosms of a chronic Ostrea edulis alkalinity-enhancement exposure experiment
DEFINE2
Bundesamt für Naturschutz
BfN
data points
Event label
Type of study
type
Tissues
Sample ID
Individual ID
Individual oyster ID
Experiment day
day
Time
Sampling date
Treatment
treatment
Tissue, dry mass
tissue
dry mass
Mass
Vial, mass
Empty
Plus digested sample weight
Sample, wet mass
Digested sample solution
wet mass
Nickel
µg/l
nickel
Density
Chromium
chromium
Cobalt
cobalt
µg/g
per tissue dry mass
ecotoxicology; ocean alkalinity enhancement; Ostrea edulis
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