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Bracher, Astrid; Hohe, Christian; Wiegmann, Sonja; Xi, Hongyan (2026): Phytoplankton pigment concentrations during Maria S. Merian cruise MSM93 from North Sea to Fram Strait and back in June to July 2020 from HPLC analysed water samples [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.995973

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Published: 2026-07-10 • DOI registered: 2026-07-10

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Abstract:
Phytoplankton pigments were determined in the water surface (from 0 to 11 m depth) of a transect from the North Sea to the Fram Strait in the Arctic Ocean and back during Maria S. Merian expedition MSM93 from 28 June to 26 July 2020. Water samples were collected from CTD Niskin bottles at five to six different depths from the upper 100 m at CTD stations and from underway sampling. Between 0.4 to 3.5 L of each seawater sample was filtered through Whatman GF/F filters. The sample filters were then shock‐frozen in liquid N2 and kept at −80 °C until analysis. High Pressure Liquid Chromatography (HPLC) was performed to quantify various phytoplankton pigments (see Table 1 in Taylor et al. 2011) following the method of Barlow et al. (1997) that was adjusted to our temperature‐controlled instruments as detailed in Álvarez et al. (2022). Data were used to analyse the Fram Strait phytoplankton community composition dynamics in Xi et al. (submitted to GBC) and contributing to an Panarctic Ocean pigment collection (Heidemann et al. 2026).
Keyword(s):
HPLC analysis; phytoplankton pigments
Supplement to:
Xi, Hongyan; et al. (submitted): Long-term satellite observations (1998–2025) reveal changing phytoplankton community structure and phenology in the Fram Strait. Global Biogeochemical Cycles
References:
Álvarez, Eva; Losa, Svetlana N; Bracher, Astrid; Thoms, Silke; Völker, Christoph (2022): Phytoplankton Light Absorption Impacted by Photoprotective Carotenoids in a Global Ocean Spectrally-resolved Biogeochemistry Model. Journal of Advances in Modeling Earth Systems, https://doi.org/10.1029/2022MS003126
Barlow, Raymond G; Cummings, Denise G; Gibb, S W (1997): Improved resolution of mono- and divinyl chlorophylls a and b and zeaxanthin and lutein in phytoplankton extracts using reverse phase C-8 HPLC. Marine Ecology Progress Series, 161, 303-307, https://doi.org/10.3354/meps161303
Heidemann, Asta C; Hayward, Alexander; Assmy, Philipp; Basu, Atreya; Bracher, Astrid; Castellani, Giulia; Ditullio, Giacomo; Dragańska-Deja, Katarzyna; Fujiwara, Amane; Fragoso, Glaucia M; Høyer, Jacob; Hwang, Jiwoon; Iversen, Morten Hvitfeldt; von Jackowski, Anabel; Juul-Pedersen, Thomas; Kowalczuk, Piotr; Lee, Youngju; Matsuoka, Atsushi; Merz, Alenya; Mundy, Christopher John; Ostermann, Else; Peeken, Ilka; Pinkerton, Matt; Stoń-Egiert, Joanna; Thielecke, Antonia; Veyssiere, Gaëlle; Xi, Hongyan; Yang, Eun Jin; Gonçalves-Araujo, Rafael (preprint): A Pan-Arctic Pigment Database for Phytoplankton and Sea-Ice Algae. https://doi.org/10.5194/essd-2026-144
Taylor, Bettina B; Torrecilla, Elena; Bernhardt, Anja; Taylor, Marc H; Peeken, Ilka; Röttgers, Rüdiger; Piera, Jaume; Bracher, Astrid (2011): Bio-optical provinces in the eastern Atlantic Ocean and their biogeographical relevance. Biogeosciences, 8(12), 3609-3629, https://doi.org/10.5194/bg-8-3609-2011
Funding:
European Commission (EC), grant/award no. 23138L03D-COP-INNO SCI-9000: Copernicus Marine – Innovation Service Evolution R&D Project ML-PhyTAO
German Research Foundation (DFG), grant/award no. 268020496: TRR 172: ArctiC Amplification: Climate Relevant Atmospheric and SurfaCe Processes, and Feedback Mechanisms
Coverage:
Median Latitude: 75.903036 * Median Longitude: -0.518131 * South-bound Latitude: 63.040350 * West-bound Longitude: -21.226470 * North-bound Latitude: 79.649390 * East-bound Longitude: 10.324530
Date/Time Start: 2020-06-28T07:02:00 * Date/Time End: 2020-07-26T16:46:18
Minimum DEPTH, water: 0 m * Maximum DEPTH, water: 12 m
Event(s):
MSM93_10-1 * Latitude: 79.000000 * Longitude: 9.000000 * Date/Time: 2020-07-03T20:49:11 * Elevation: -200.4 m * Location: North Greenland Sea * Campaign: MSM93 (GPF 18-1_33) * Basis: Maria S. Merian * Method/Device: CTD/Rosette (CTD-RO)
MSM93_12-1 * Latitude: 79.009000 * Longitude: 8.330000 * Date/Time: 2020-07-03T22:48:46 * Elevation: -789.8 m * Location: North Greenland Sea * Campaign: MSM93 (GPF 18-1_33) * Basis: Maria S. Merian * Method/Device: CTD/Rosette (CTD-RO)
MSM93_13-6 * Latitude: 79.000000 * Longitude: 8.298000 * Date/Time: 2020-07-04T15:44:58 * Elevation: -819.8 m * Location: North Greenland Sea * Campaign: MSM93 (GPF 18-1_33) * Basis: Maria S. Merian * Method/Device: CTD/Rosette (CTD-RO)
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Sample IDSample IDBracher, Astrid'HPLC
2Event labelEventBracher, Astrid
3DEPTH, waterDepth watermBracher, AstridGeocode
4DATE/TIMEDate/TimeBracher, AstridGeocode
5LATITUDELatitudeBracher, AstridGeocode
6LONGITUDELongitudeBracher, AstridGeocode
7Chlorophyllide bChlide bµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)Chlorolide b
8Chlorophyll c3Chl c3µg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
9Chlorophyll c1+c2Chl c1+c2µg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
10Chlorophyllide aChlide aµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)Chlorolide a
11PeridininPeridµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
12Pheophorbide aPhide aµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
1319-ButanoyloxyfucoxanthinBut-fucoµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
14FucoxanthinFucoµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
15NeoxanthinNeoµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
1619-HexanoyloxyfucoxanthinHex-fucoµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
17PrasinoxanthinPrasµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
18ViolaxanthinViolaµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
19DiadinoxanthinDiadinoµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
20AntheraxanthinAntheraµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
21AlloxanthinAlloµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
22DiatoxanthinDiatoµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
23ZeaxanthinZeaµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
24LuteinLutµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
25Chlorophyll bChl bµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
26Chlorophyll aChl aµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
27alpha-Carotenea-Carµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
28beta-Caroteneb-Carµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
2919'-Hexanoyloxy-4-ketofucoxanthin19Hex-4-kfucoµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
30Pheophytin aPhytin aµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
31Pyropheophytin aPyrophytin aµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
5694 data points

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