https://doi.pangaea.de/10.1594/PANGAEA.995912Bienhold, ChristinaChristinaBienhold0000-0003-2269-9468Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, BremerhavenMax Planck Institute for Marine MicrobiologyPANGAEA2026chlorophyll-aDeep seaEurasian Basinphaeopigmentssediment biogeochemistryEvent labelLatitude of eventLongitude of eventElevation of eventDate/Time of eventReplicate, biologicalDepth, sediment/rock, top/minimumDepth, sediment/rock, bottom/maximumDEPTH, sediment/rockChlorophyll aPhaeopigmentsCommentMulticorer with televisionMultiCorerLaboratory fluorometer, Turner Designs, TrilogyARK-XXVII/3Polarstern2012-08-09T10:35:00/2012-09-29T08:45:00Dataset10.3389/fmars.2022.100495910.2312/BzPM_0663_201310.1016/S0967-0637(97)00052-610.1594/PANGAEA.80319510.1594/PANGAEA.849043484 data pointstext/tab-separated-valuesCreative Commons Attribution 4.0 InternationalUndisturbed sediment samples were obtained with a TV-guided multiple corer (TV-MUC). Upon retrieval of the MUC, the overlying water was carefully removed from each core and the upper few centimeters of sediment were cut using a steel plate and a custom-made plastic ring into the following depth horizons: 0-1 cm, 1-5 cm, 5-10 cm. For each sediment horizon, 3 biological replicates were obtained.

Chloroplastic pigments were extracted from 1 ml sediment with 3x8 ml acetone (90%), including the addition of glass beads and grinding of the samples in a cell mill Boetius and Damm (1998, doi: 10.1016/S0967-0637(97)00052-6). Samples were centrifuged and chlorophyll a and phaeopigment (after addition of 30 μl 20% HCl) concentrations were determined in the supernatant by fluorometric measurement with a Turner Trilogy fluorometer with the "Chlorophyll a Acidification" fluorescence module. Chlorophyll a and phaeopigment concentrations were calculated according to Bienhold et al. 2022 (doi: 10.3389/fmars.2022.1004959). Chloroplastic pigment equivalents (CPE) are calculated as the sum of chlorophyll a and phaeopigment concentrations.

This is an updated dataset referencing the following two PANGAEA datasets:
Bienhold, Christina; Boetius, Antje (2013): Pigment concentration in surface sediments collected during POLARSTERN cruise ARK-XXVII/3 (IceArc) [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.803195, In supplement to: Boetius, Antje; Albrecht, Sebastian; Bakker, Karel; Bienhold, Christina; Felden, Janine; Fernández-Méndez, Mar; Hendricks, Stefan; Katlein, Christian; Lalande, Catherine; Krumpen, Thomas; Nicolaus, Marcel; Peeken, Ilka; Rabe, Benjamin; Rogacheva, Antonina; Rybakova, Elena; Somavilla Cabrillo, Raquel; Wenzhöfer, Frank; ARK-XXVII/3 Shipboard Scientific Party (2013): Export of algal biomass from the melting Arctic sea ice. Science, 339(6126), 1430-1432, https://doi.org/10.1126/science.1231346
and
Bienhold, Christina; Boetius, Antje (2015): Chlorophyll pigments of the central Arctic Ocean during POLARSTERN cruise ARK-XXVII/3 from August-September 2012 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.849043

Original calculations overestimated phaeopigment concentrations, and consequently chloroplastic pigment equivalents (CPE), as they referred to an inaccurate calibration factor. Chlorophyll values remain unchanged. Relative differences between samples remain unchanged as well. We updated the dataset with the adjusted calculations, following the approach described in Bienhold et al. 2022 (https://doi.org/10.3389/fmars.2022.1004959). In addition, we now also report individual measurements from 3 biological replicates, rather than averages across replicates.17.73532130.858881.876388.82675Arctic Ocean