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Bienhold, Christina (2026): Surface sediment chloroplastic pigments from Polarstern expedition PS80 to the Central Arctic Ocean during August-September 2012 [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.995912 (DOI registration in progress)

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Published: 2026-08-28

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Abstract:
Undisturbed sediment samples were obtained with a TV-guided multiple corer (TV-MUC). Upon retrieval of the MUC, the overlying water was carefully removed from each core and the upper few centimeters of sediment were cut using a steel plate and a custom-made plastic ring into the following depth horizons: 0-1 cm, 1-5 cm, 5-10 cm. For each sediment horizon, 3 biological replicates were obtained.
Chloroplastic pigments were extracted from 1 ml sediment with 3x8 ml acetone (90%), including the addition of glass beads and grinding of the samples in a cell mill Boetius and Damm (1998, doi: 10.1016/S0967-0637(97)00052-6). Samples were centrifuged and chlorophyll a and phaeopigment (after addition of 30 μl 20% HCl) concentrations were determined in the supernatant by fluorometric measurement with a Turner Trilogy fluorometer with the "Chlorophyll a Acidification" fluorescence module. Chlorophyll a and phaeopigment concentrations were calculated according to Bienhold et al. 2022 (doi: 10.3389/fmars.2022.1004959). Chloroplastic pigment equivalents (CPE) are calculated as the sum of chlorophyll a and phaeopigment concentrations.
This is an updated dataset referencing the following two PANGAEA datasets:
Bienhold, Christina; Boetius, Antje (2013): Pigment concentration in surface sediments collected during POLARSTERN cruise ARK-XXVII/3 (IceArc) [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.803195, In supplement to: Boetius, Antje; Albrecht, Sebastian; Bakker, Karel; Bienhold, Christina; Felden, Janine; Fernández-Méndez, Mar; Hendricks, Stefan; Katlein, Christian; Lalande, Catherine; Krumpen, Thomas; Nicolaus, Marcel; Peeken, Ilka; Rabe, Benjamin; Rogacheva, Antonina; Rybakova, Elena; Somavilla Cabrillo, Raquel; Wenzhöfer, Frank; ARK-XXVII/3 Shipboard Scientific Party (2013): Export of algal biomass from the melting Arctic sea ice. Science, 339(6126), 1430-1432, https://doi.org/10.1126/science.1231346
and
Bienhold, Christina; Boetius, Antje (2015): Chlorophyll pigments of the central Arctic Ocean during POLARSTERN cruise ARK-XXVII/3 from August-September 2012 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.849043
Original calculations overestimated phaeopigment concentrations, and consequently chloroplastic pigment equivalents (CPE), as they referred to an inaccurate calibration factor. Chlorophyll values remain unchanged. Relative differences between samples remain unchanged as well. We updated the dataset with the adjusted calculations, following the approach described in Bienhold et al. 2022 (https://doi.org/10.3389/fmars.2022.1004959). In addition, we now also report individual measurements from 3 biological replicates, rather than averages across replicates.
Keyword(s):
chlorophyll-a; Deep sea; Eurasian Basin; phaeopigments; sediment biogeochemistry
Related to:
Bienhold, Christina; Schourup-Kristensen, Vibe; Krumpen, Thomas; Nöthig, Eva-Maria; Wenzhöfer, Frank; Korhonen, Meri; Vredenborg, Myriel; Hehemann, Laura; Boetius, Antje (2022): Effects of sea ice retreat and ocean warming on the Laptev Sea continental slope ecosystem (1993 vs 2012). Frontiers in Marine Science, 9, 1004959, https://doi.org/10.3389/fmars.2022.1004959
Boetius, Antje (2013): The Expedition of the Research Vessel Polarstern to the Arctic in 2012 (ARK-XXVII/3). Berichte zur Polar- und Meeresforschung = Reports on Polar and Marine Research, 663, 166 pp, https://doi.org/10.2312/BzPM_0663_2013
Boetius, Antje; Damm, Ellen (1998): Benthic oxygen uptake, hydrolytic potentials and microbial biomass at the Arctic continental slope. Deep Sea Research Part I: Oceanographic Research Papers, 45(2-3), 239-275, https://doi.org/10.1016/S0967-0637(97)00052-6
Previous version:
Bienhold, Christina; Boetius, Antje (2013): Pigment concentration in surface sediments collected during POLARSTERN cruise ARK-XXVII/3 (IceArc) [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.803195
Bienhold, Christina; Boetius, Antje (2015): Chlorophyll pigments of the central Arctic Ocean during POLARSTERN cruise ARK-XXVII/3 from August-September 2012 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.849043
Coverage:
Median Latitude: 84.647959 * Median Longitude: 82.079667 * South-bound Latitude: 81.876300 * West-bound Longitude: 17.735320 * North-bound Latitude: 88.826750 * East-bound Longitude: 130.858800
Date/Time Start: 2012-08-09T10:35:00 * Date/Time End: 2012-09-29T08:45:00
Minimum DEPTH, sediment/rock: 0.005 m * Maximum DEPTH, sediment/rock: 0.075 m
Event(s):
PS80/225-1  * Latitude: 84.047320 * Longitude: 31.192630 * Date/Time: 2012-08-09T10:35:00 * Elevation: -4019.2 m * Location: Arctic Ocean * Campaign: ARK-XXVII/3 (PS80 IceArc) * Basis: Polarstern * Method/Device: Multicorer with television (TVMUC) * Comment: SL=4013m; Position sensor: USBL Ixsea Posidonia
PS80/240-1  * Latitude: 83.947330 * Longitude: 76.872000 * Date/Time: 2012-08-15T07:50:00 * Elevation: -4807.7 m * Location: Arctic Ocean * Campaign: ARK-XXVII/3 (PS80 IceArc) * Basis: Polarstern * Method/Device: Multicorer with television (TVMUC) * Comment: 3431 m GE 52.1; Position sensor: Ship's GPS
PS80/260-1  * Latitude: 82.876830 * Longitude: 109.862500 * Date/Time: 2012-08-21T07:44:00 * Elevation: -3588.9 m * Location: Arctic Ocean * Campaign: ARK-XXVII/3 (PS80 IceArc) * Basis: Polarstern * Method/Device: Multicorer with television (TVMUC) * Comment: max 3591 m; Position sensor: Ship's GPS
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
Event labelEvent
Latitude of eventLatitude
Longitude of eventLongitude
Elevation of eventElevationm
Date/Time of eventDate/Time
Replicate, biologicalRepl bioBienhold, Christina
Depth, sediment/rock, top/minimumDepth sed topmBienhold, Christina
Depth, sediment/rock, bottom/maximumDepth sed botmBienhold, Christina
DEPTH, sediment/rockDepth sedmBienhold, ChristinaGeocode
10 Chlorophyll aChl aµg/cm3Bienhold, ChristinaLaboratory fluorometer, Turner Designs, Trilogy
11 PhaeopigmentsPhaeµg/cm3Bienhold, ChristinaLaboratory fluorometer, Turner Designs, Trilogy
12 CommentCommentBienhold, Christina
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
484 data points

Data

Download dataset as tab-delimited text — use the following character encoding:


Event

Latitude

Longitude

Elevation [m]

Date/Time

Repl bio

Depth sed top [m]

Depth sed bot [m]

Depth sed [m]
10 
Chl a [µg/cm3]
11 
Phae [µg/cm3]
12 
Comment
PS80/225-1 84.047331.1926-40192012-08-09T10:35:0010.000.010.0050.0490.220Ice station #1
PS80/225-184.047331.1926-40192012-08-09T10:35:0020.000.010.0050.0600.236Ice station #1
PS80/225-184.047331.1926-40192012-08-09T10:35:0030.000.010.0050.0770.297Ice station #1
PS80/225-184.047331.1926-40192012-08-09T10:35:0010.010.050.0300.0060.144Ice station #1
PS80/225-184.047331.1926-40192012-08-09T10:35:0020.010.050.0300.0170.136Ice station #1
PS80/225-184.047331.1926-40192012-08-09T10:35:0030.010.050.0300.0170.174Ice station #1
PS80/225-184.047331.1926-40192012-08-09T10:35:0010.050.100.0750.0150.154Ice station #1
PS80/225-184.047331.1926-40192012-08-09T10:35:0020.050.100.0750.0270.121Ice station #1
PS80/225-184.047331.1926-40192012-08-09T10:35:0030.050.100.0750.0320.139Ice station #1
PS80/240-1 83.947376.8720-48082012-08-15T07:50:0010.000.010.0050.1680.525Ice station #2
PS80/240-183.947376.8720-48082012-08-15T07:50:0020.000.010.0050.2510.559Ice station #2
PS80/240-183.947376.8720-48082012-08-15T07:50:0030.000.010.0050.2150.363Ice station #2
PS80/240-183.947376.8720-48082012-08-15T07:50:0010.010.050.0300.0480.295Ice station #2
PS80/240-183.947376.8720-48082012-08-15T07:50:0020.010.050.0300.0330.236Ice station #2
PS80/240-183.947376.8720-48082012-08-15T07:50:0030.010.050.0300.0320.320Ice station #2
PS80/240-183.947376.8720-48082012-08-15T07:50:0010.050.100.0750.0210.117Ice station #2
PS80/240-183.947376.8720-48082012-08-15T07:50:0020.050.100.0750.0180.160Ice station #2
PS80/240-183.947376.8720-48082012-08-15T07:50:0030.050.100.0750.0240.140Ice station #2
PS80/260-1 82.8768109.8625-35892012-08-21T07:44:0010.000.010.0050.1720.320Ice station #3
PS80/260-182.8768109.8625-35892012-08-21T07:44:0020.000.010.0050.2970.398Ice station #3
PS80/260-182.8768109.8625-35892012-08-21T07:44:0030.000.010.0050.0810.214Ice station #3
PS80/260-182.8768109.8625-35892012-08-21T07:44:0010.010.050.0300.0350.092Ice station #3
PS80/260-182.8768109.8625-35892012-08-21T07:44:0020.010.050.0300.0300.096Ice station #3
PS80/260-182.8768109.8625-35892012-08-21T07:44:0030.010.050.0300.0290.106Ice station #3
PS80/260-182.8768109.8625-35892012-08-21T07:44:0010.050.100.0750.0110.053Ice station #3
PS80/260-182.8768109.8625-35892012-08-21T07:44:0020.050.100.0750.0170.047Ice station #3
PS80/260-182.8768109.8625-35892012-08-21T07:44:0030.050.100.0750.0160.055Ice station #3
PS80/277-2 82.8797130.0620-41662012-08-25T11:57:0010.000.010.0050.3050.473Ice station #4
PS80/277-282.8797130.0620-41662012-08-25T11:57:0020.000.010.0050.1410.258Ice station #4
PS80/277-282.8797130.0620-41662012-08-25T11:57:0030.000.010.0050.1260.247Ice station #4
PS80/277-282.8797130.0620-41662012-08-25T11:57:0010.010.050.0300.0270.083Ice station #4
PS80/277-282.8797130.0620-41662012-08-25T11:57:0020.010.050.0300.0290.106Ice station #4
PS80/277-282.8797130.0620-41662012-08-25T11:57:0030.010.050.0300.0260.186Ice station #4
PS80/277-282.8797130.0620-41662012-08-25T11:57:0010.050.100.0750.0110.054Ice station #4
PS80/277-282.8797130.0620-41662012-08-25T11:57:0020.050.100.0750.0140.056Ice station #4
PS80/277-282.8797130.0620-41662012-08-25T11:57:0030.050.100.0750.0080.054Ice station #4
PS80/330-1 81.8763130.8588-40342012-09-05T13:03:0010.000.010.0050.1560.314Ice station #5
PS80/330-181.8763130.8588-40342012-09-05T13:03:0020.000.010.0050.1120.209Ice station #5
PS80/330-181.8763130.8588-40342012-09-05T13:03:0030.000.010.0050.1080.206Ice station #5
PS80/330-181.8763130.8588-40342012-09-05T13:03:0010.010.050.0300.0280.091Ice station #5
PS80/330-181.8763130.8588-40342012-09-05T13:03:0020.010.050.0300.0400.116Ice station #5
PS80/330-181.8763130.8588-40342012-09-05T13:03:0030.010.050.0300.0580.127Ice station #5
PS80/330-181.8763130.8588-40342012-09-05T13:03:0010.050.100.0750.0090.050Ice station #5
PS80/330-181.8763130.8588-40342012-09-05T13:03:0020.050.100.0750.0100.067Ice station #5
PS80/330-181.8763130.8588-40342012-09-05T13:03:0030.050.100.0750.0170.058Ice station #5
PS80/338-1 85.0939122.3367-43572012-09-07T14:51:0010.000.010.0050.0610.157Ice station #6
PS80/338-185.0939122.3367-43572012-09-07T14:51:0020.000.010.0050.0770.207Ice station #6
PS80/338-185.0939122.3367-43572012-09-07T14:51:0030.000.010.0050.0680.169Ice station #6
PS80/338-185.0939122.3367-43572012-09-07T14:51:0010.010.050.0300.0180.070Ice station #6
PS80/338-185.0939122.3367-43572012-09-07T14:51:0020.010.050.0300.0200.066Ice station #6
PS80/338-185.0939122.3367-43572012-09-07T14:51:0030.010.050.0300.0200.068Ice station #6
PS80/338-185.0939122.3367-43572012-09-07T14:51:0010.050.100.0750.0080.043Ice station #6
PS80/338-185.0939122.3367-43572012-09-07T14:51:0020.050.100.0750.0070.040Ice station #6
PS80/338-185.0939122.3367-43572012-09-07T14:51:0030.050.100.0750.0060.047Ice station #6
PS80/350-1 87.933461.1696-43812012-09-18T11:25:0010.000.010.0050.0530.165Ice station #7
PS80/350-187.933461.1696-43812012-09-18T11:25:0020.000.010.0050.0950.243Ice station #7
PS80/350-187.933461.1696-43812012-09-18T11:25:0030.000.010.0050.0900.224Ice station #7
PS80/350-187.933461.1696-43812012-09-18T11:25:0010.010.050.0300.0080.082Ice station #7
PS80/350-187.933461.1696-43812012-09-18T11:25:0020.010.050.0300.0190.085Ice station #7
PS80/350-187.933461.1696-43812012-09-18T11:25:0030.010.050.0300.0200.075Ice station #7
PS80/350-187.933461.1696-43812012-09-18T11:25:0010.050.100.075Ice station #7
PS80/350-187.933461.1696-43812012-09-18T11:25:0020.050.100.0750.0070.069Ice station #7
PS80/350-187.933461.1696-43812012-09-18T11:25:0030.050.100.0750.0030.069Ice station #7
PS80/361-1 88.826858.6275-43732012-09-22T07:45:0010.000.010.0050.1630.340Ice station #8
PS80/361-188.826858.6275-43732012-09-22T07:45:0020.000.010.0050.0800.272Ice station #8
PS80/361-188.826858.6275-43732012-09-22T07:45:0030.000.010.0050.1080.262Ice station #8
PS80/361-188.826858.6275-43732012-09-22T07:45:0010.010.050.0300.0150.078Ice station #8
PS80/361-188.826858.6275-43732012-09-22T07:45:0020.010.050.0300.0250.107Ice station #8
PS80/361-188.826858.6275-43732012-09-22T07:45:0030.010.050.0300.0170.083Ice station #8
PS80/361-188.826858.6275-43732012-09-22T07:45:0010.050.100.0750.0060.044Ice station #8
PS80/361-188.826858.6275-43732012-09-22T07:45:0020.050.100.0750.0070.041Ice station #8
PS80/361-188.826858.6275-43732012-09-22T07:45:0030.050.100.0750.0080.065Ice station #8
PS80/394-1 84.350117.7353-40232012-09-29T08:45:0010.000.010.0050.0430.313Ice station #9
PS80/394-184.350117.7353-40232012-09-29T08:45:0020.000.010.0050.0620.317Ice station #9
PS80/394-184.350117.7353-40232012-09-29T08:45:0030.000.010.0050.0450.267Ice station #9
PS80/394-184.350117.7353-40232012-09-29T08:45:0010.010.050.0300.0180.265Ice station #9
PS80/394-184.350117.7353-40232012-09-29T08:45:0020.010.050.0300.0090.311Ice station #9
PS80/394-184.350117.7353-40232012-09-29T08:45:0030.010.050.0300.0190.260Ice station #9
PS80/394-184.350117.7353-40232012-09-29T08:45:0010.050.100.0750.0060.304Ice station #9
PS80/394-184.350117.7353-40232012-09-29T08:45:0020.050.100.0750.0110.278Ice station #9
PS80/394-184.350117.7353-40232012-09-29T08:45:0030.050.100.0750.0060.306Ice station #9