PANGAEA_995819 Seawater carbonate chemistry and response of seasonal marine microbial communities (phytoplankton, bacteria) and viruses from the Santa Barbara Channel to OAE Gately, James A; Kim, Sylvia M; Welch, Zoe S; Martínez, Joaquín Martínez; Catlett, Dylan; Jin, Benjamin; Manzagol, Madeline; Larson, Angela; Brzezinski, Mark A; Iglesias-Rodriguez, Maria D Seawater carbonate chemistry and response of seasonal marine microbial communities (phytoplankton, bacteria) and viruses from the Santa Barbara Channel to OAE 2026-07-06 PANGAEA Dataset https://doi.pangaea.de/10.1594/PANGAEA.995819 Investigator James A Gately jgately@ucsb.edu Investigator Yan Yang yangyan@xmu.edu.cn Earth Science Type of study Sampling date File name Number Sample code/label Treatment Cryptophytes Nanoeukaryotes Picoeukaryotes Synechococcus Bacteria Virion-like particles Alkalinity, total Volume Temperature, water Temperature, water, standard deviation pH, total scale pH, standard deviation Salinity Alkalinity, total Carbon, inorganic, dissolved Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Bicarbonate ion Carbonate ion Carbon dioxide Calcite saturation state Aragonite saturation state Revelle factor Carbon, inorganic, particulate Phosphate Phosphate Nitrate and Nitrite Ammonia Nitrogen, inorganic, dissolved Silicate Silicate Biogenic silica Carbon, organic, particulate Nitrogen, organic, particulate Carbonate system computation flag geoscientificInformation Biomass/Abundance/Elemental composition Coast and continental shelf Community composition and diversity Containers and aquaria (20-1000 L or < 1 m**2) Entire community Laboratory experiment North Pacific Ocean alkalinity enhancement Pelagos Temperate Spectrophotometric Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Complete OA-ICC Ocean Acidification International Coordination Centre unrestricted CC-BY-4.0: Creative Commons Attribution 4.0 International English PANGAEA Data Publisher for Earth & Environmental Science https://www.pangaea.de/ Data Center Contact Michael Diepenbroek info@pangaea.de

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Bremen Bremen 28359 Germany online 31838 data points text/tab-separated-values Gately, James A; Kim, Sylvia M; Welch, Zoe S; Martínez, Joaquín Martínez; Catlett, Dylan; Jin, Benjamin; Manzagol, Madeline; Larson, Angela; Brzezinski, Mark A; Iglesias-Rodriguez, Maria D (2026): Abrupt alkalinization alters microbial diversity and promotes the proliferation of marine parasites in coastal microcosm experiments. ICES Journal of Marine Science, 83(5), fsag063, https://doi.org/10.1093/icesjms/fsag063 Gately, James A; Kim, Sylvia M; Welch, Zoe S; Martínez Martínez, Joaquín; Catlett, Dylan; Jin, Benjamin; Manzagol, Madeline; Larson, Angela; Brzezinski, Mark A; Iglesias-Rodriguez, Maria D (2026): Data from: Abrupt alkalinization alters microbial diversity and promotes the proliferation of marine parasites in coastal microcosm experiments [dataset]. Dryad, https://doi.org/10.5061/DRYAD.73N5TB381 Nisumaa, Anne-Marin; Pesant, Stephane; Bellerby, Richard G J; Delille, Bruno; Middelburg, Jack J; Orr, James C; Riebesell, Ulf; Tyrrell, Toby; Wolf-Gladrow, Dieter A; Gattuso, Jean-Pierre (2010): EPOCA/EUR-OCEANS data compilation on the biological and biogeochemical responses to ocean acidification. Earth System Science Data, 2(2), 167-175, https://doi.org/10.5194/essd-2-167-2010 Yang, Yan; Brockmann, Patrick; Galdino, Carolina; Schindler, Uwe; Gazeau, Frédéric (2024): An update of data compilation on the biological response to ocean acidification and overview of the OA-ICC data portal. Earth System Science Data, 16(8), 3771-3780, https://doi.org/10.5194/essd-16-3771-2024 Yang, Yan; Hansson, L; Gattuso, Jean-Pierre (2016): Data compilation on the biological response to ocean acidification: an update. Earth System Science Data, 8(1), 79-87, https://doi.org/10.5194/essd-8-79-2016 Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2024): seacarb: seawater carbonate chemistry with R. R package version 3.3.3. https://cran.r-project.org/web/packages/seacarb/index.html

Here, we used outdoor microcosm exper- iments to investigate the effec t s of abrupt limestone-inspired and NaOH alkalinity additions of ∼750 μmol/kg, reflecting model-predicted ocean alkalinity enhancement (OAE) scenarios that produce severe localized impacts (e.g. large variations in pH and Omega). We assess the response of seasonal marine microbial communities (phytoplankton, bacteria) and viruses from the Santa Barbara Channel, analyzed by high-throughput amplicon sequencing and flow cytometry. This dataset is included in the OA-ICC data compilation maintained in the framework of the IAEA Ocean Acidification International Coordination Centre (see https://oa-icc.ipsl.fr). Original data were downloaded from Dryad Digital Repository (see Source) by the OA-ICC data curator. In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2024) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2026-07-03. ** For all details see the full metadata description at "https://doi.pangaea.de/10.1594/PANGAEA.995819"!

http://mel.xmu.edu.cn/en/ https://cran.r-project.org/web/packages/seacarb/index.html seacarb: seawater carbonate chemistry with R. R package version 3.3.3 https://doi.org/10.1093/icesjms/fsag063 Abrupt alkalinization alters microbial diversity and promotes the proliferation of marine parasites in coastal microcosm experiments https://doi.org/10.5061/DRYAD.73N5TB381 Data from: Abrupt alkalinization alters microbial diversity and promotes the proliferation of marine parasites in coastal microcosm experiments https://doi.org/10.5194/essd-16-3771-2024 An update of data compilation on the biological response to ocean acidification and overview of the OA-ICC data portal https://doi.org/10.5194/essd-2-167-2010 Calculated using seacarb after Nisumaa et al. (2010) https://doi.org/10.5194/essd-2-167-2010 EPOCA/EUR-OCEANS data compilation on the biological and biogeochemical responses to ocean acidification https://doi.org/10.5194/essd-8-79-2016 Data compilation on the biological response to ocean acidification: an update https://www.iaea.org/services/oa-icc/science-and-collaboration/data-access-and-management OA-ICC DIF 9.4 2026-07-06 2026-08-04