García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven AWI https://www.awi.de/ https://ror.org/032e6b942 Bedulina Daria S daria.bedulina@gmail.com 0000-0001-7650-0974 Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven AWI https://www.awi.de/ https://ror.org/032e6b942 Sokolova Inna M inna.sokolova@uni-rostock.de 0000-0002-2068-4302 University of Rostock https://www.uni-rostock.de/en/ https://ror.org/03zdwsf69 Lannig Gisela Gisela.Lannig@awi.de https://www.awi.de/en/about-us/organisation/staff/single-view/gisela-lannig.html 0000-0002-9210-256X Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven AWI https://www.awi.de/ https://ror.org/032e6b942 2026 Behavioral, metabolomic, heat shock protein 70, and catalase responses of Littorina littorea to acute experimental warming in Helgoland (North Sea, Germany) during November 2023 and June 2024 dataset https://doi.pangaea.de/10.1594/PANGAEA.995770 2026-07-27T09:35:40 Adult Littorina littorea were collected from the intertidal zone of Helgoland (North Sea, Germany; 54°11′ N, 7°53′ E) during November 2023 and June 2024 to investigate physiological and behavioral responses to acute warming. After collection, the organisms were transported to the Alfred Wegener Institute in Bremerhaven, Germany, and acclimated for two weeks to season-specific temperatures representative of winter (10 °C) and summer (16 °C) in aquarium systems containing filtered, recirculating, and continuously aerated seawater at a salinity of 33. Animals were fed Fucus vesiculosus ad libitum throughout the acclimation period. Following acclimation, individuals were exposed to a continuous warming ramp from the respective seasonal acclimation temperature to 34 °C at a rate of 1.5 °C h⁻¹. Samples were collected at predefined temperature intervals between 10 and 34 °C for winter-acclimated animals and between 16 and 34 °C for summer-acclimated animals.The dataset contains behavioral, metabolomic, and biochemical measurements obtained from snails at the respective experimental temperatures. Behavioral observations include the time required for antenna extension and the initiation of locomotion. Metabolomic data were obtained from whole-body soft-tissue extracts using proton nuclear magnetic resonance spectroscopy using the ultra-shielded vertical 9.4 T NMR spectrometer (Advance III HD 400 WB, Bruker-BioSpin GmbH, Germany), with a 1.7 mm triple tuned 1H-13C-15N NMR probe and a Carr-Purcell-Meiboom-Gill sequence (Bruker protocol cpmgpr1d, TOPSPIN 3.5, Bruker GmbH, Germany) with water suppression. Biochemical measurements comprise heat shock protein 70 abundance and catalase activity determined from whole-body tissue homogenates. The data were collected to characterize temperature-dependent physiological and behavioral responses of L. littorea across seasons and to identify thermal thresholds during acute warming exposure. García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Bedulina Daria S daria.bedulina@gmail.com 0000-0001-7650-0974 Sokolova Inna A 0000-0002-2068-4302 Lannig Gisela Gisela.Lannig@awi.de https://www.awi.de/en/about-us/organisation/staff/single-view/gisela-lannig.html 0000-0002-9210-256X 2026 Seasonal impact on the thermal tolerance window of a keystone coastal grazer, the common periwinkle Littorina littorea journal article Journal of Comparative Physiology B-Biochemical Systemic and Environmentalphysiology https://doi.org/10.1007/s00360-026-01670-3 7.883131 8.58048 53.53325 54.188876 8.2318055 53.861063 2023-11-03T12:00:00 2024-06-27T07:00:00 Littorina_littorea_exp_summer 53.53325 8.58048 2024-06-27T07:00:00 Laboratory experiment Experimental analyse at the Alfred Wegner Institute Bremerhaven Littorina_littorea_exp_winter 53.53325 8.58048 2024-04-09T07:00:00 Laboratory experiment Experimental analyse at the Alfred Wegner Institute Bremerhaven Littorina_littorea_sampling_summer 54.188876 7.883131 2024-06-04T12:00:00 Biological sample BIOS Gastropods of the species Littorina littorea were collected during low tide in the Nordstrand, Helgoland by foot during low tide Littorina_littorea_sampling_winter 54.188876 7.883131 2023-11-03T12:00:00 Biological sample BIOS Gastropods of the species Littorina littorea were collected during low tide in the Nordstrand, Helgoland by foot during low tide 5496 CC-BY-4.0 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ Event label Event García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Event Type of study Study type type García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Study type Species, unique identification Species UID species García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Species UID Species, unique identification (Semantic URI) Species UID (Semantic URI) species García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Species UID (Semantic URI) Species, unique identification (URI) Species UID (URI) species García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Species UID (URI) Sample ID Sample ID García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Sample ID Treatment: temperature T:temp °C treatment Temperature Θ http://dbpedia.org/resource/Temperature Fiber-optic thermometer, Weidmann Optocon, FOTEMP1-4 García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 T:temp [°C] (Fiber-optic thermometer, Weid...) Time in seconds Time sec s http://en.wikipedia.org/wiki/Time Time T http://dbpedia.org/resource/Time Body camera, Hoestr, A39 García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Time to show antennas Time sec [s] (Time to show antennas, Body c...) Time in seconds Time sec s http://en.wikipedia.org/wiki/Time Time T http://dbpedia.org/resource/Time Body camera, Hoestr, A39 García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Start of movement Time sec [s] (Start of movement, Body camer...) Aminobutyrate Aminobutyrate mmol/l Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Aminobutyrate [mmol/l] (NMR spectrometer, Bruker-BioS...) Adenylates, total Adenylates mmol/l adenylates total https://www.oxforddictionaries.com/definition/american_english/total Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Adenylates [mmol/l] (NMR spectrometer, Bruker-BioS...) Acetate Acetate mmol/l acetate http://purl.obolibrary.org/obo/CHEBI_30089 Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Acetate [mmol/l] (NMR spectrometer, Bruker-BioS...) Alanine Ala mmol/l alanine http://purl.obolibrary.org/obo/CHEBI_16449 Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Ala [mmol/l] (NMR spectrometer, Bruker-BioS...) Arginine Arg mmol/l arginine http://purl.obolibrary.org/obo/CHEBI_29016 Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Arg [mmol/l] (NMR spectrometer, Bruker-BioS...) Betaine Betaine mmol/l betaine https://en.wikipedia.org/wiki/Betaine Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Betaine [mmol/l] (NMR spectrometer, Bruker-BioS...) Carnitine Carnitine mmol/l carnitine http://purl.obolibrary.org/obo/CHEBI_17126 Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Carnitine [mmol/l] (NMR spectrometer, Bruker-BioS...) Choline Choline mmol/l choline http://purl.obolibrary.org/obo/CHEBI_15354 Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Choline [mmol/l] (NMR spectrometer, Bruker-BioS...) Fumarate Fumarate mmol/l Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Fumarate [mmol/l] (NMR spectrometer, Bruker-BioS...) Glucose Glc mmol/l glucose http://purl.obolibrary.org/obo/CHEBI_17234 Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Glc [mmol/l] (NMR spectrometer, Bruker-BioS...) Glutamate Glutamate mmol/l Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Glutamate [mmol/l] (NMR spectrometer, Bruker-BioS...) Glutamine Gln mmol/l glutamine http://purl.obolibrary.org/obo/CHEBI_28300 Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Gln [mmol/l] (NMR spectrometer, Bruker-BioS...) Glutathione GSH mmol/l glutathione http://purl.obolibrary.org/obo/CHEBI_16856 Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 GSH [mmol/l] (NMR spectrometer, Bruker-BioS...) Homarine Homarine mmol/l https://pubchem.ncbi.nlm.nih.gov/compound/Homarine Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Homarine [mmol/l] (NMR spectrometer, Bruker-BioS...) Hypotaurine Hypotaurine mmol/l https://en.wikipedia.org/wiki/Hypotaurine hypotaurine http://purl.obolibrary.org/obo/CHEBI_16668 Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Hypotaurine [mmol/l] (NMR spectrometer, Bruker-BioS...) Isoleucine Ile mmol/l isoleucine http://purl.obolibrary.org/obo/CHEBI_24898 Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Ile [mmol/l] (NMR spectrometer, Bruker-BioS...) Lactate Lactate mmol/l lactate http://purl.obolibrary.org/obo/CHEBI_24996 Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Lactate [mmol/l] (NMR spectrometer, Bruker-BioS...) Leucine Leu mmol/l leucine http://purl.obolibrary.org/obo/CHEBI_25017 Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Leu [mmol/l] (NMR spectrometer, Bruker-BioS...) Maleate Maleate mmol/l maleate http://purl.obolibrary.org/obo/CHEBI_132951 Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Maleate [mmol/l] (NMR spectrometer, Bruker-BioS...) Maltose Maltose mmol/l maltose http://purl.obolibrary.org/obo/CHEBI_17306 Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Maltose [mmol/l] (NMR spectrometer, Bruker-BioS...) Methionine Met mmol/l methionine http://purl.obolibrary.org/obo/CHEBI_16811 Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Met [mmol/l] (NMR spectrometer, Bruker-BioS...) O-Acetylcholine O-Acetylcholine mmol/l Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 O-Acetylcholine [mmol/l] (NMR spectrometer, Bruker-BioS...) O-Phosphocholine O-Phosphocholine mmol/l Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 O-Phosphocholine [mmol/l] (NMR spectrometer, Bruker-BioS...) Propionate Propionate mmol/l propionate http://purl.obolibrary.org/obo/CHEBI_17272 Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Propionate [mmol/l] (NMR spectrometer, Bruker-BioS...) Pyruvate Pyruvate mmol/l pyruvate http://purl.obolibrary.org/obo/CHEBI_15361 Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Pyruvate [mmol/l] (NMR spectrometer, Bruker-BioS...) Serine Ser mmol/l serine http://purl.obolibrary.org/obo/CHEBI_17822 Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Ser [mmol/l] (NMR spectrometer, Bruker-BioS...) Succinate Succinate mmol/l succinate http://purl.obolibrary.org/obo/CHEBI_26806 Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Succinate [mmol/l] (NMR spectrometer, Bruker-BioS...) Taurine Tau mmol/l taurine http://purl.obolibrary.org/obo/CHEBI_15891 Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Tau [mmol/l] (NMR spectrometer, Bruker-BioS...) Trimethylamine N-oxide TMAO mmol/l trimethylamine N-oxide http://purl.obolibrary.org/obo/CHEBI_15724 Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 TMAO [mmol/l] (NMR spectrometer, Bruker-BioS...) Valine Val mmol/l valine http://purl.obolibrary.org/obo/CHEBI_27266 Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 Val [mmol/l] (NMR spectrometer, Bruker-BioS...) sn-Glycero-3-phosphocholine sn-Glycero-3-phosphocholine mmol/l https://pubchem.ncbi.nlm.nih.gov/compound/SN-glycero-3-phosphocholine Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 sn-Glycero-3-phosphocholine [mmol/l] (NMR spectrometer, Bruker-BioS...) beta-Alanine b-ala mmol/l beta-alanine http://purl.obolibrary.org/obo/CHEBI_16958 Molar Concentration NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 b-ala [mmol/l] (NMR spectrometer, Bruker-BioS...) Heat-shock protein 70 Hsp70 arbitrary units Protein separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE); followed by immunoblotting García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 An integrative control sample (a pooled sample from all individuals) was included on each gel and used as an internal reference. Hsp70 levels were expressed in arbitrary units relative to this control Hsp70 [arbitrary units] (An integrative control sample...) Catalase, unit per protein mass CAT/prot U/mg https://en.wikipedia.org/wiki/Catalase catalase https://www.qmul.ac.uk/sbcs/iubmb/enzyme/EC1/11/1/6.html Specific Activity http://search.ebscohost.com/login.aspx?direct=true&db=nlebk&AN=98508&site=ehost-live&ebv=EB&ppid=pp_610 Microplate reader, BioTek, Synergy H1 García Tomás tomas.garcia@awi.de 0009-0008-5533-5912 CAT/prot [U/mg] (Microplate reader, BioTek, Sy...) CurationLevelC Enhanced curation https://wiki.pangaea.de/wiki/Curation_levels Behavioral response Gastropod Laboratory experiment metabolomics oxidative stress Seasonal acclimatization warming author118219 author38738 author38740 author54409 author57529 curlevel30 event3417259 event3417260 event3417261 event3417262 geocode1599 geocode1600 geocode1601 inst21448 inst32 journal8417 license21 method10780 method15768 method19357 method19358 method19361 method19362 method8239 param105163 param125539 param16065 param185520 param186894 param186896 param186897 param186899 param186900 param186901 param186902 param186906 param186907 param186909 param186911 param186912 param186913 param186914 param186920 param186923 param186924 param186925 param186929 param186932 param45228 param510679 param521296 param521298 param524038 param524039 param524047 param537587 param565733 param565735 param565737 param567631 param567632 param567633 param567634 pi118219 ref140500 term1073587 term1073698 term1073714 term1073750 term1073752 term1073755 term1073761 term1073762 term1073768 term1073901 term1073957 term1074348 term1074350 term1074636 term2584350 term2605558 term2607001 term2607377 term2611368 term2612889 term2614814 term2623790 term2625866 term38253 term38471 term38555 term38841 term39153 term41013 term43972 term43975 term44030 term44032 term47421 term49384 term49506 term50039 term50312 term51692 term78276 term78277