de.pangaea.dataset995770 und dataset PANGAEA

info@pangaea.de

https://www.pangaea.de/ information pointOfContact 2026-07-27T09:35:42Z https://doi.pangaea.de/10.1594/PANGAEA.995770 2026-07-27T09:35:40 publication García, Tomás

tomas.garcia@awi.de

https://orcid.org/0009-0008-5533-5912 information author Bedulina, Daria S

daria.bedulina@gmail.com

https://orcid.org/0000-0001-7650-0974 information author Sokolova, Inna M

inna.sokolova@uni-rostock.de

https://orcid.org/0000-0002-2068-4302 information author Lannig, Gisela

Gisela.Lannig@awi.de

https://orcid.org/0000-0002-9210-256X information author PANGAEA

info@pangaea.de

https://www.pangaea.de/ information publisher tableDigital Adult Littorina littorea were collected from the intertidal zone of Helgoland (North Sea, Germany; 54°11′ N, 7°53′ E) during November 2023 and June 2024 to investigate physiological and behavioral responses to acute warming. After collection, the organisms were transported to the Alfred Wegener Institute in Bremerhaven, Germany, and acclimated for two weeks to season-specific temperatures representative of winter (10 °C) and summer (16 °C) in aquarium systems containing filtered, recirculating, and continuously aerated seawater at a salinity of 33. Animals were fed Fucus vesiculosus ad libitum throughout the acclimation period. Following acclimation, individuals were exposed to a continuous warming ramp from the respective seasonal acclimation temperature to 34 °C at a rate of 1.5 °C h⁻¹. Samples were collected at predefined temperature intervals between 10 and 34 °C for winter-acclimated animals and between 16 and 34 °C for summer-acclimated animals.The dataset contains behavioral, metabolomic, and biochemical measurements obtained from snails at the respective experimental temperatures. Behavioral observations include the time required for antenna extension and the initiation of locomotion. Metabolomic data were obtained from whole-body soft-tissue extracts using proton nuclear magnetic resonance spectroscopy using the ultra-shielded vertical 9.4 T NMR spectrometer (Advance III HD 400 WB, Bruker-BioSpin GmbH, Germany), with a 1.7 mm triple tuned 1H-13C-15N NMR probe and a Carr-Purcell-Meiboom-Gill sequence (Bruker protocol cpmgpr1d, TOPSPIN 3.5, Bruker GmbH, Germany) with water suppression. Biochemical measurements comprise heat shock protein 70 abundance and catalase activity determined from whole-body tissue homogenates. The data were collected to characterize temperature-dependent physiological and behavioral responses of L. littorea across seasons and to identify thermal thresholds during acute warming exposure. completed García, Tomás

tomas.garcia@awi.de

https://orcid.org/0009-0008-5533-5912 information principalInvestigator Behavioral response Gastropod Laboratory experiment metabolomics oxidative stress Seasonal acclimatization warming theme 1995-01-01 creation PANGAEA

info@pangaea.de

https://www.pangaea.de/ information pointOfContact copyright copyright CC-BY-4.0: Creative Commons Attribution 4.0 International textTable und geoscientificInformation 7.883131 8.58048 53.53325 54.188876 2023-11-03T12:00:00 2024-06-27T07:00:00 Event label thematicClassification Type of study thematicClassification Species, unique identification thematicClassification Species, unique identification (Semantic URI) thematicClassification Species, unique identification (URI) thematicClassification Sample ID thematicClassification Treatment: temperature physicalMeasurement Fiber-optic thermometer, Weidmann Optocon, FOTEMP1-4 °C °C Time in seconds physicalMeasurement Body camera, Hoestr, A39; Time to show antennas s s Time in seconds physicalMeasurement Body camera, Hoestr, A39; Start of movement s s Aminobutyrate physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l Adenylates, total physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l Acetate physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l Alanine physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l Arginine physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l Betaine physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l Carnitine physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l Choline physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l Fumarate physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l Glucose physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l Glutamate physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l Glutamine physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l Glutathione physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l Homarine physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l Hypotaurine physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l Isoleucine physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l Lactate physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l Leucine physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l Maleate physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l Maltose physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l Methionine physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l O-Acetylcholine physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l O-Phosphocholine physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l Propionate physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l Pyruvate physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l Serine physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l Succinate physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l Taurine physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l Trimethylamine N-oxide physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l Valine physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l sn-Glycero-3-phosphocholine physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l beta-Alanine physicalMeasurement NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB mmol/l mmol/l Heat-shock protein 70 physicalMeasurement Protein separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE); followed by immunoblotting; An integrative control sample (a pooled sample from all individuals) was included on each gel and used as an internal reference. Hsp70 levels were expressed in arbitrary units relative to this control arbitrary units arbitrary units Catalase, unit per protein mass physicalMeasurement Microplate reader, BioTek, Synergy H1 U/mg U/mg text/tab-separated-values unknown https://doi.pangaea.de/10.1594/PANGAEA.995770?format=textfile download dataset The data set was checked for completeness, correctness, and consistency of metainformation. Validity of used methods was checked and - if applicable - precision and range of data. EVENT LABEL: Littorina_littorea_exp_summer * LATITUDE: 53.533250 * LONGITUDE: 8.580480 * DATE/TIME: 2024-06-27T07:00:00 * METHOD/DEVICE: Laboratory experiment Experimental analyse at the Alfred Wegner Institute Bremerhaven 2024-06-27T07:00:00 EVENT LABEL: Littorina_littorea_exp_winter * LATITUDE: 53.533250 * LONGITUDE: 8.580480 * DATE/TIME: 2024-04-09T07:00:00 * METHOD/DEVICE: Laboratory experiment Experimental analyse at the Alfred Wegner Institute Bremerhaven 2024-04-09T07:00:00 EVENT LABEL: Littorina_littorea_sampling_summer * LATITUDE: 54.188876 * LONGITUDE: 7.883131 * DATE/TIME: 2024-06-04T12:00:00 * METHOD/DEVICE: Biological sample Gastropods of the species Littorina littorea were collected during low tide in the Nordstrand, Helgoland by foot during low tide 2024-06-04T12:00:00 EVENT LABEL: Littorina_littorea_sampling_winter * LATITUDE: 54.188876 * LONGITUDE: 7.883131 * DATE/TIME: 2023-11-03T12:00:00 * METHOD/DEVICE: Biological sample Gastropods of the species Littorina littorea were collected during low tide in the Nordstrand, Helgoland by foot during low tide 2023-11-03T12:00:00 Related to 2026-01-01 publication https://doi.org/10.1007/s00360-026-01670-3 García, Tomás

tomas.garcia@awi.de

https://orcid.org/0009-0008-5533-5912 information author Bedulina, Daria S

daria.bedulina@gmail.com

https://orcid.org/0000-0001-7650-0974 information author Sokolova, Inna A https://orcid.org/0000-0002-2068-4302 information author Lannig, Gisela

Gisela.Lannig@awi.de

https://orcid.org/0000-0002-9210-256X information author documentDigital Journal of Comparative Physiology B-Biochemical Systemic and Environmentalphysiology