PANGAEA_995770
Behavioral, metabolomic, heat shock protein 70, and catalase responses of Littorina littorea to acute experimental warming in Helgoland (North Sea, Germany) during November 2023 and June 2024
García, Tomás; Bedulina, Daria S; Sokolova, Inna M; Lannig, Gisela
Behavioral, metabolomic, heat shock protein 70, and catalase responses of Littorina littorea to acute experimental warming in Helgoland (North Sea, Germany) during November 2023 and June 2024
2026-07-27
PANGAEA
Dataset
https://doi.pangaea.de/10.1594/PANGAEA.995770
Investigator
Tomás
García
tomas.garcia@awi.de
Earth Science
Event label
Type of study
Species, unique identification
Species, unique identification (Semantic URI)
Species, unique identification (URI)
Sample ID
Treatment: temperature
Time in seconds
Aminobutyrate
Adenylates, total
Acetate
Alanine
Arginine
Betaine
Carnitine
Choline
Fumarate
Glucose
Glutamate
Glutamine
Glutathione
Homarine
Hypotaurine
Isoleucine
Lactate
Leucine
Maleate
Maltose
Methionine
O-Acetylcholine
O-Phosphocholine
Propionate
Pyruvate
Serine
Succinate
Taurine
Trimethylamine N-oxide
Valine
sn-Glycero-3-phosphocholine
beta-Alanine
Heat-shock protein 70
Catalase, unit per protein mass
geoscientificInformation
Behavioral response
Biological sample
Gastropod
Laboratory experiment
Littorina_littorea_exp_summer
Littorina_littorea_exp_winter
Littorina_littorea_sampling_summer
Littorina_littorea_sampling_winter
metabolomics
oxidative stress
Seasonal acclimatization
warming
Fiber-optic thermometer, Weidmann Optocon, FOTEMP1-4
Body camera, Hoestr, A39
NMR spectrometer, Bruker-BioSpin, Advance III HD 400 WB
Protein separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE); followed by immunoblotting
Microplate reader, BioTek, Synergy H1
2023-11-03
2024-06-27
Complete
53.53325
54.188876
7.883131
8.58048
unrestricted
CC-BY-4.0: Creative Commons Attribution 4.0 International
English
PANGAEA
Data Publisher for Earth & Environmental Science
https://www.pangaea.de/
Data Center Contact
Michael
Diepenbroek
info@pangaea.de
Leobener Str.
Bremen
Bremen
28359
Germany
online
5496 data points
text/tab-separated-values
García, Tomás; Bedulina, Daria S; Sokolova, Inna A; Lannig, Gisela (2026): Seasonal impact on the thermal tolerance window of a keystone coastal grazer, the common periwinkle Littorina littorea. Journal of Comparative Physiology B-Biochemical Systemic and Environmentalphysiology, https://doi.org/10.1007/s00360-026-01670-3
Adult Littorina littorea were collected from the intertidal zone of Helgoland (North Sea, Germany; 54°11′ N, 7°53′ E) during November 2023 and June 2024 to investigate physiological and behavioral responses to acute warming. After collection, the organisms were transported to the Alfred Wegener Institute in Bremerhaven, Germany, and acclimated for two weeks to season-specific temperatures representative of winter (10 °C) and summer (16 °C) in aquarium systems containing filtered, recirculating, and continuously aerated seawater at a salinity of 33. Animals were fed Fucus vesiculosus ad libitum throughout the acclimation period. Following acclimation, individuals were exposed to a continuous warming ramp from the respective seasonal acclimation temperature to 34 °C at a rate of 1.5 °C h⁻¹. Samples were collected at predefined temperature intervals between 10 and 34 °C for winter-acclimated animals and between 16 and 34 °C for summer-acclimated animals.The dataset contains behavioral, metabolomic, and biochemical measurements obtained from snails at the respective experimental temperatures. Behavioral observations include the time required for antenna extension and the initiation of locomotion. Metabolomic data were obtained from whole-body soft-tissue extracts using proton nuclear magnetic resonance spectroscopy using the ultra-shielded vertical 9.4 T NMR spectrometer (Advance III HD 400 WB, Bruker-BioSpin GmbH, Germany), with a 1.7 mm triple tuned 1H-13C-15N NMR probe and a Carr-Purcell-Meiboom-Gill sequence (Bruker protocol cpmgpr1d, TOPSPIN 3.5, Bruker GmbH, Germany) with water suppression. Biochemical measurements comprise heat shock protein 70 abundance and catalase activity determined from whole-body tissue homogenates. The data were collected to characterize temperature-dependent physiological and behavioral responses of L. littorea across seasons and to identify thermal thresholds during acute warming exposure. ** For all details see the full metadata description at "https://doi.pangaea.de/10.1594/PANGAEA.995770"!
http://en.wikipedia.org/wiki/Time
Time in seconds
https://doi.org/10.1007/s00360-026-01670-3
Seasonal impact on the thermal tolerance window of a keystone coastal grazer, the common periwinkle Littorina littorea
https://en.wikipedia.org/wiki/Catalase
Catalase, unit per protein mass
https://en.wikipedia.org/wiki/Hypotaurine
Hypotaurine
https://pubchem.ncbi.nlm.nih.gov/compound/Homarine
Homarine
https://pubchem.ncbi.nlm.nih.gov/compound/SN-glycero-3-phosphocholine
sn-Glycero-3-phosphocholine
DIF
9.4
2026-07-27
2026-07-27