/* DATA DESCRIPTION: Citation: Gao, Guang; Qu, Liming; Ge, Jingke; Gao, Kunshan (2026): Seawater carbonate chemistry and physiological data of the diatom Thalassiosira weissflogii employed in studying the diel effects of ocean acidification [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.995743 (DOI registration in progress) Abstract: We comparatively investigated the physiological and transcriptional performances of a diatom Thalassiosira weissflogii acclimated to OA (pHt drop of 0.35–0.41) between day and night periods. This dataset is included in the OA-ICC data compilation maintained in the framework of the IAEA Ocean Acidification International Coordination Centre (see https://oa-icc.ipsl.fr). Original data were provided by the author of the related paper (see Related to) to the OA-ICC data curator. In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2024) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2026-07-01. Keyword(s): Bottles or small containers/Aquaria (<20 L); Chromista; Gene expression (incl. proteomics); Growth/Morphology; Heterokontophyta; Laboratory experiment; Laboratory strains; Light; Not applicable; Ocean acidification; Pelagos; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species; Thalassiosira weissflogii Related to: Gao, Guang; Qu, Liming; Ge, Jingke; Gao, Kunshan (2026): Divergent responses of the diatom Thalassiosira weissflogii to ocean acidification during light and dark periods. Limnology and Oceanography Letters, 11(3), e70128, https://doi.org/10.1002/lol2.70128 Source: Gao, Guang; Qu, Liming; Ge, Jingke; Gao, Kunshan (2026): Diel effects of ocean acidification on diatom [dataset]. Mendeley Data, https://doi.org/10.17632/CJWPPWD3M5.1 References: Nisumaa, Anne-Marin; Pesant, Stephane; Bellerby, Richard G J; Delille, Bruno; Middelburg, Jack J; Orr, James C; Riebesell, Ulf; Tyrrell, Toby; Wolf-Gladrow, Dieter A; Gattuso, Jean-Pierre (2010): EPOCA/EUR-OCEANS data compilation on the biological and biogeochemical responses to ocean acidification. Earth System Science Data, 2(2), 167-175, https://doi.org/10.5194/essd-2-167-2010 Yang, Yan; Brockmann, Patrick; Galdino, Carolina; Schindler, Uwe; Gazeau, Frédéric (2024): An update of data compilation on the biological response to ocean acidification and overview of the OA-ICC data portal. Earth System Science Data, 16(8), 3771-3780, https://doi.org/10.5194/essd-16-3771-2024 Yang, Yan; Hansson, L; Gattuso, Jean-Pierre (2016): Data compilation on the biological response to ocean acidification: an update. Earth System Science Data, 8(1), 79-87, https://doi.org/10.5194/essd-8-79-2016 Related code / software: Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2024): seacarb: seawater carbonate chemistry with R. R package version 3.3.3. https://cran.r-project.org/web/packages/seacarb/index.html Project(s): Ocean Acidification International Coordination Centre (OA-ICC) (URI: https://www.iaea.org/services/oa-icc/science-and-collaboration/data-access-and-management) Parameter(s): Type of study (Study type) * PI: Gao, Guang (https://orcid.org/0000-0002-9011-9640, gaog@hhit.edu.cn) Species, unique identification (Species UID) * PI: Gao, Guang (https://orcid.org/0000-0002-9011-9640, gaog@hhit.edu.cn) Treatment (Treat) * PI: Gao, Guang (https://orcid.org/0000-0002-9011-9640, gaog@hhit.edu.cn) * COMMENT: AC=ambient, HC=high CO2 Growth rate [1/h] (µ) * PI: Gao, Guang (https://orcid.org/0000-0002-9011-9640, gaog@hhit.edu.cn) * COMMENT: per 12 h, dark period Growth rate [1/h] (µ) * PI: Gao, Guang (https://orcid.org/0000-0002-9011-9640, gaog@hhit.edu.cn) * COMMENT: per 12 h, light period Growth rate [1/day] (µ) * PI: Gao, Guang (https://orcid.org/0000-0002-9011-9640, gaog@hhit.edu.cn) Respiration rate, oxygen, per cell [fmol/#/h] (Resp O2/cell) * PI: Gao, Guang (https://orcid.org/0000-0002-9011-9640, gaog@hhit.edu.cn) * COMMENT: Dark period Respiration rate, oxygen, per cell [fmol/#/h] (Resp O2/cell) * PI: Gao, Guang (https://orcid.org/0000-0002-9011-9640, gaog@hhit.edu.cn) * COMMENT: Light period Net photosynthesis rate, oxygen, per cell [fmol/#/h] (PN O2/cell) * PI: Gao, Guang (https://orcid.org/0000-0002-9011-9640, gaog@hhit.edu.cn) Gross photosynthesis rate, oxygen, per cell [pmol/#/h] (PG O2/cell) * PI: Gao, Guang (https://orcid.org/0000-0002-9011-9640, gaog@hhit.edu.cn) Gene abundance [#] (GA) * PI: Gao, Guang (https://orcid.org/0000-0002-9011-9640, gaog@hhit.edu.cn) * COMMENT: upregulated differential expression genes (DEGs) Gene abundance [#] (GA) * PI: Gao, Guang (https://orcid.org/0000-0002-9011-9640, gaog@hhit.edu.cn) * COMMENT: downregulated differential expression genes (DEGs) pH, total scale (pHT) * PI: Gao, Guang (https://orcid.org/0000-0002-9011-9640, gaog@hhit.edu.cn) * COMMENT: light Alkalinity, total [µmol/kg] (AT) * PI: Gao, Guang (https://orcid.org/0000-0002-9011-9640, gaog@hhit.edu.cn) * COMMENT: light Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) [µatm] (pCO2water_SST_wet) * PI: Gao, Guang (https://orcid.org/0000-0002-9011-9640, gaog@hhit.edu.cn) * METHOD/DEVICE: Calculated using CO2SYS * COMMENT: light Carbon, inorganic, dissolved [µmol/kg] (DIC) * PI: Gao, Guang (https://orcid.org/0000-0002-9011-9640, gaog@hhit.edu.cn) * METHOD/DEVICE: Calculated using CO2SYS * COMMENT: light Bicarbonate ion [µmol/kg] ([HCO3]-) * PI: Gao, Guang (https://orcid.org/0000-0002-9011-9640, gaog@hhit.edu.cn) * METHOD/DEVICE: Calculated using CO2SYS * COMMENT: light Carbonate ion [µmol/kg] ([CO3]2-) * PI: Gao, Guang (https://orcid.org/0000-0002-9011-9640, gaog@hhit.edu.cn) * METHOD/DEVICE: Calculated using CO2SYS * COMMENT: light Carbon dioxide [µmol/kg] (CO2) * PI: Gao, Guang (https://orcid.org/0000-0002-9011-9640, gaog@hhit.edu.cn) * METHOD/DEVICE: Calculated using CO2SYS * COMMENT: light Carbonate system computation flag (CSC flag) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: light Carbon dioxide [µmol/kg] (CO2) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: light Fugacity of carbon dioxide (water) at sea surface temperature (wet air) [µatm] (fCO2water_SST_wet) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: light Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) [µatm] (pCO2water_SST_wet) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: light Bicarbonate ion [µmol/kg] ([HCO3]-) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: light Carbonate ion [µmol/kg] ([CO3]2-) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: light Carbon, inorganic, dissolved [µmol/kg] (DIC) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: light Aragonite saturation state (Omega Arg) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: light Calcite saturation state (Omega Cal) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: light pH, total scale (pHT) * PI: Gao, Guang (https://orcid.org/0000-0002-9011-9640, gaog@hhit.edu.cn) * COMMENT: dark Alkalinity, total [µmol/kg] (AT) * PI: Gao, Guang (https://orcid.org/0000-0002-9011-9640, gaog@hhit.edu.cn) * COMMENT: dark Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) [µatm] (pCO2water_SST_wet) * PI: Gao, Guang (https://orcid.org/0000-0002-9011-9640, gaog@hhit.edu.cn) * METHOD/DEVICE: Calculated using CO2SYS * COMMENT: dark Carbon, inorganic, dissolved [µmol/kg] (DIC) * PI: Gao, Guang (https://orcid.org/0000-0002-9011-9640, gaog@hhit.edu.cn) * METHOD/DEVICE: Calculated using CO2SYS * COMMENT: dark Bicarbonate ion [µmol/kg] ([HCO3]-) * PI: Gao, Guang (https://orcid.org/0000-0002-9011-9640, gaog@hhit.edu.cn) * METHOD/DEVICE: Calculated using CO2SYS * COMMENT: dark Carbonate ion [µmol/kg] ([CO3]2-) * PI: Gao, Guang (https://orcid.org/0000-0002-9011-9640, gaog@hhit.edu.cn) * METHOD/DEVICE: Calculated using CO2SYS * COMMENT: dark Carbon dioxide [µmol/kg] (CO2) * PI: Gao, Guang (https://orcid.org/0000-0002-9011-9640, gaog@hhit.edu.cn) * METHOD/DEVICE: Calculated using CO2SYS * COMMENT: dark Carbonate system computation flag (CSC flag) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: dark Carbon dioxide [µmol/kg] (CO2) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: dark Fugacity of carbon dioxide (water) at sea surface temperature (wet air) [µatm] (fCO2water_SST_wet) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: dark Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) [µatm] (pCO2water_SST_wet) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: dark Bicarbonate ion [µmol/kg] ([HCO3]-) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: dark Carbonate ion [µmol/kg] ([CO3]2-) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: dark Carbon, inorganic, dissolved [µmol/kg] (DIC) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: dark Aragonite saturation state (Omega Arg) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: dark Calcite saturation state (Omega Cal) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: dark License: Creative Commons Attribution 4.0 International (CC-BY-4.0) (URI: https://creativecommons.org/licenses/by/4.0/) Status: Curation Level: Enhanced curation (URI: https://wiki.pangaea.de/wiki/Curation_levels) Size: 487 data points */ Study type Species UID Treat (AC=ambient, HC=high CO2) µ [1/h] (per 12 h, dark period) µ [1/h] (per 12 h, light period) µ [1/day] Resp O2/cell [fmol/#/h] (Dark period) Resp O2/cell [fmol/#/h] (Light period) PN O2/cell [fmol/#/h] PG O2/cell [pmol/#/h] GA [#] (upregulated differential expr...) GA [#] (downregulated differential ex...) pHT (light) AT [µmol/kg] (light) pCO2water_SST_wet [µatm] (light, Calculated using CO2SYS) DIC [µmol/kg] (light, Calculated using CO2SYS) [HCO3]- [µmol/kg] (light, Calculated using CO2SYS) [CO3]2- [µmol/kg] (light, Calculated using CO2SYS) CO2 [µmol/kg] (light, Calculated using CO2SYS) CSC flag (light, Calculated using seaca...) CO2 [µmol/kg] (light, Calculated using seaca...) fCO2water_SST_wet [µatm] (light, Calculated using seaca...) pCO2water_SST_wet [µatm] (light, Calculated using seaca...) [HCO3]- [µmol/kg] (light, Calculated using seaca...) [CO3]2- [µmol/kg] (light, Calculated using seaca...) DIC [µmol/kg] (light, Calculated using seaca...) Omega Arg (light, Calculated using seaca...) Omega Cal (light, Calculated using seaca...) pHT (dark) AT [µmol/kg] (dark) pCO2water_SST_wet [µatm] (dark, Calculated using CO2SYS) DIC [µmol/kg] (dark, Calculated using CO2SYS) [HCO3]- [µmol/kg] (dark, Calculated using CO2SYS) [CO3]2- [µmol/kg] (dark, Calculated using CO2SYS) CO2 [µmol/kg] (dark, Calculated using CO2SYS) CSC flag (dark, Calculated using seacar...) CO2 [µmol/kg] (dark, Calculated using seacar...) fCO2water_SST_wet [µatm] (dark, Calculated using seacar...) pCO2water_SST_wet [µatm] (dark, Calculated using seacar...) [HCO3]- [µmol/kg] (dark, Calculated using seacar...) [CO3]2- [µmol/kg] (dark, Calculated using seacar...) DIC [µmol/kg] (dark, Calculated using seacar...) Omega Arg (dark, Calculated using seacar...) Omega Cal (dark, Calculated using seacar...) laboratory Thalassiosira weissflogii AC-1 0.462210898 0.579 1.041210898 8.16 2283.28 290.39 1952.96 1725.71 217.82 9.43 8 9.52 292.04 293.03 1741.76 219.89 1971.16 3.44 5.30 8.04 2274.55 398.21 2010.20 1820.59 176.67 12.93 8 13.04 400.06 401.42 1835.68 178.17 2026.89 2.79 4.29 laboratory Thalassiosira weissflogii AC-2 0.466242666 0.614 1.080242666 8.16 2283.28 290.39 1952.96 1725.71 217.82 9.43 8 9.52 292.04 293.03 1741.76 219.89 1971.16 3.44 5.30 8.04 2274.55 398.21 2010.20 1820.59 176.67 12.93 8 13.04 400.06 401.42 1835.68 178.17 2026.89 2.79 4.29 laboratory Thalassiosira weissflogii AC-3 0.491104678 0.585 1.076104678 8.16 2283.28 290.39 1952.96 1725.71 217.82 9.43 8 9.52 292.04 293.03 1741.76 219.89 1971.16 3.44 5.30 8.04 2274.55 398.21 2010.20 1820.59 176.67 12.93 8 13.04 400.06 401.42 1835.68 178.17 2026.89 2.79 4.29 laboratory Thalassiosira weissflogii HC-1 0.428036783 0.677 1.105036783 7.80 2295.88 761.68 2142.18 2005.37 112.07 24.73 8 24.91 764.62 767.22 2018.84 112.76 2156.51 1.76 2.72 7.64 2294.91 1165.70 2203.82 2086.66 79.31 37.85 8 38.08 1168.82 1172.80 2098.98 79.76 2216.82 1.25 1.92 laboratory Thalassiosira weissflogii HC-2 0.376298215 0.627 1.003298215 7.80 2295.88 761.68 2142.18 2005.37 112.07 24.73 8 24.91 764.62 767.22 2018.84 112.76 2156.51 1.76 2.72 7.64 2294.91 1165.70 2203.82 2086.66 79.31 37.85 8 38.08 1168.82 1172.80 2098.98 79.76 2216.82 1.25 1.92 laboratory Thalassiosira weissflogii HC-3 0.418036783 0.647 1.065036783 7.80 2295.88 761.68 2142.18 2005.37 112.07 24.73 8 24.91 764.62 767.22 2018.84 112.76 2156.51 1.76 2.72 7.64 2294.91 1165.70 2203.82 2086.66 79.31 37.85 8 38.08 1168.82 1172.80 2098.98 79.76 2216.82 1.25 1.92 laboratory Thalassiosira weissflogii AC-1 140.6211947 176.3 1623.3 1.7996 8.16 2283.28 290.39 1952.96 1725.71 217.82 9.43 8 9.52 292.04 293.03 1741.76 219.89 1971.16 3.44 5.30 8.04 2274.55 398.21 2010.20 1820.59 176.67 12.93 8 13.04 400.06 401.42 1835.68 178.17 2026.89 2.79 4.29 laboratory Thalassiosira weissflogii AC-2 130.6748887 165.2 1722.6 1.8878 8.16 2283.28 290.39 1952.96 1725.71 217.82 9.43 8 9.52 292.04 293.03 1741.76 219.89 1971.16 3.44 5.30 8.04 2274.55 398.21 2010.20 1820.59 176.67 12.93 8 13.04 400.06 401.42 1835.68 178.17 2026.89 2.79 4.29 laboratory Thalassiosira weissflogii AC-3 127.1294543 180.2 1658.3 1.8385 8.16 2283.28 290.39 1952.96 1725.71 217.82 9.43 8 9.52 292.04 293.03 1741.76 219.89 1971.16 3.44 5.30 8.04 2274.55 398.21 2010.20 1820.59 176.67 12.93 8 13.04 400.06 401.42 1835.68 178.17 2026.89 2.79 4.29 laboratory Thalassiosira weissflogii HC-1 151.5198330 196.1 1902.3 2.0984 7.80 2295.88 761.68 2142.18 2005.37 112.07 24.73 8 24.91 764.62 767.22 2018.84 112.76 2156.51 1.76 2.72 7.64 2294.91 1165.70 2203.82 2086.66 79.31 37.85 8 38.08 1168.82 1172.80 2098.98 79.76 2216.82 1.25 1.92 laboratory Thalassiosira weissflogii HC-2 147.7447277 197.8 1821.3 2.0191 7.80 2295.88 761.68 2142.18 2005.37 112.07 24.73 8 24.91 764.62 767.22 2018.84 112.76 2156.51 1.76 2.72 7.64 2294.91 1165.70 2203.82 2086.66 79.31 37.85 8 38.08 1168.82 1172.80 2098.98 79.76 2216.82 1.25 1.92 laboratory Thalassiosira weissflogii HC-3 155.4225085 180.3 1756.6 1.9369 7.80 2295.88 761.68 2142.18 2005.37 112.07 24.73 8 24.91 764.62 767.22 2018.84 112.76 2156.51 1.76 2.72 7.64 2294.91 1165.70 2203.82 2086.66 79.31 37.85 8 38.08 1168.82 1172.80 2098.98 79.76 2216.82 1.25 1.92 laboratory Thalassiosira weissflogii HC-L vs AC-L 4074 2871 laboratory Thalassiosira weissflogii HC-D vs AC-D 1908 1907 laboratory Thalassiosira weissflogii AC-L vs AC-D 20470 13390 laboratory Thalassiosira weissflogii HC-L vs HC-D 21042 10239 laboratory Thalassiosira weissflogii HC-L vs AC-D 18055 12575