PANGAEA_995588 Seawater carbonate chemistry and calcifying fluid chemistry of Crassostrea virginica Unzueta-Martínez, Andrea; Delaney, Jennifer A; Morkeski, K; Ross, Abby; Wang, Zhaohui Aleck; Girguis, Peter R Seawater carbonate chemistry and calcifying fluid chemistry of Crassostrea virginica 2026-06-18 PANGAEA Dataset https://doi.pangaea.de/10.1594/PANGAEA.995588 Investigator Andrea Unzueta-Martínez andrea_unzuetamartinez@fas.harvard.edu Investigator Yan Yang yangyan@xmu.edu.cn Earth Science Type of study Species, unique identification Treatment Local Time Replicates Salinity Salinity, standard deviation Temperature, water Temperature, water, standard deviation pH, NBS scale pH, standard deviation Carbon, inorganic, dissolved Carbon, inorganic, dissolved, standard deviation Alkalinity, total Alkalinity, total, standard deviation Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Partial pressure of carbon dioxide, standard deviation Carbonate ion Carbonate ion, standard deviation Bicarbonate ion Bicarbonate ion, standard deviation Carbon dioxide Carbon dioxide, standard deviation Calcite saturation state Calcite saturation state, standard deviation Aragonite saturation state Aragonite saturation state, standard deviation Calcifying fluid, pH Calcifying fluid, pH, standard deviation Calcifying fluid, dissolved inorganic carbon Calcifying fluid, dissolved inorganic carbon, standard deviation Calcifying fluid, total alkalinity Calcifying fluid, total alkalinity, standard deviation Calcifying fluid, partial pressure of carbon dioxide Calcifying fluid, partial pressure of carbon dioxide, standard deviation Calcifying fluid, carbonate ion Calcifying fluid, carbonate ion, standard deviation Calcifying fluid, bicarbonate ion Calcifying fluid, bicarbonate ion, standard deviation Calcifying fluid, carbon dioxide Calcifying fluid, carbon dioxide, standard deviation Calcifying fluid, calcite saturation state Calcifying fluid, calcite saturation state, standard deviation Calcifying fluid, aragonite saturation state Calcifying fluid, aragonite saturation state, standard deviation Carbonate system computation flag pH, total scale Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) geoscientificInformation Acid-base regulation Animalia Barnstable_OA Benthic animals Benthos Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Crassostrea virginica Entire community Experiment Laboratory experiment Mollusca North Atlantic Ocean acidification Single species Temperate Potentiometric Calculated with seacarb package in R Calculated using seacarb after Nisumaa et al. (2010) Complete 41.7104 41.7104 -70.3051 -70.3051 OA-ICC Ocean Acidification International Coordination Centre unrestricted CC-BY-4.0: Creative Commons Attribution 4.0 International English PANGAEA Data Publisher for Earth & Environmental Science https://www.pangaea.de/ Data Center Contact Michael Diepenbroek info@pangaea.de

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Bremen Bremen 28359 Germany online 1030 data points text/tab-separated-values Unzueta-Martínez, Andrea; Delaney, Jennifer A; Morkeski, K; Ross, Abby; Wang, Zhaohui Aleck; Girguis, Peter R (2026): Coexpression among eastern oyster host and microbiome genes suggests coordinated regulation of calcifying fluid chemistry. Proceedings of the National Academy of Sciences of the United States of America, 123(11), e2521539123, https://doi.org/10.1073/pnas.2521539123 Nisumaa, Anne-Marin; Pesant, Stephane; Bellerby, Richard G J; Delille, Bruno; Middelburg, Jack J; Orr, James C; Riebesell, Ulf; Tyrrell, Toby; Wolf-Gladrow, Dieter A; Gattuso, Jean-Pierre (2010): EPOCA/EUR-OCEANS data compilation on the biological and biogeochemical responses to ocean acidification. Earth System Science Data, 2(2), 167-175, https://doi.org/10.5194/essd-2-167-2010 Yang, Yan; Brockmann, Patrick; Galdino, Carolina; Schindler, Uwe; Gazeau, Frédéric (2024): An update of data compilation on the biological response to ocean acidification and overview of the OA-ICC data portal. Earth System Science Data, 16(8), 3771-3780, https://doi.org/10.5194/essd-16-3771-2024 Yang, Yan; Hansson, L; Gattuso, Jean-Pierre (2016): Data compilation on the biological response to ocean acidification: an update. Earth System Science Data, 8(1), 79-87, https://doi.org/10.5194/essd-8-79-2016 Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2024): seacarb: seawater carbonate chemistry with R. R package version 3.3.3. https://cran.r-project.org/web/packages/seacarb/index.html GitHub - Andrea-Unzueta-Martinez/oyster-host-microbiome-transcriptomics: Count matrices and scripts for oyster host and microbiome transcriptomic analyses. · GitHub [webpage]. https://github.com/Andrea-Unzueta-Martinez/oyster-host-microbiome-transcriptomics

To better understand oysters' molecular mechanisms and the role of their calcifying-fluid microbes, we conducted experiments that simulated a tidal cycle, measured calcifying fluid pH and total dissolved inorganic carbon, and characterized host and microbial gene expression via transcriptomics. This dataset is included in the OA-ICC data compilation maintained in the framework of the IAEA Ocean Acidification International Coordination Centre (see https://oa-icc.ipsl.fr). Original data were extracted from tables in the related paper (see Related to) by the OA-ICC data curator. In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2024) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2026-06-18. ** For all details see the full metadata description at "https://doi.pangaea.de/10.1594/PANGAEA.995588"!

http://mel.xmu.edu.cn/en/ https://cran.r-project.org/web/packages/seacarb/index.html seacarb: seawater carbonate chemistry with R. R package version 3.3.3 https://doi.org/10.1073/pnas.2521539123 Coexpression among eastern oyster host and microbiome genes suggests coordinated regulation of calcifying fluid chemistry https://doi.org/10.5194/essd-16-3771-2024 An update of data compilation on the biological response to ocean acidification and overview of the OA-ICC data portal https://doi.org/10.5194/essd-2-167-2010 Calculated using seacarb after Nisumaa et al. (2010) https://doi.org/10.5194/essd-2-167-2010 EPOCA/EUR-OCEANS data compilation on the biological and biogeochemical responses to ocean acidification https://doi.org/10.5194/essd-8-79-2016 Data compilation on the biological response to ocean acidification: an update https://github.com/Andrea-Unzueta-Martinez/oyster-host-microbiome-transcriptomics GitHub - Andrea-Unzueta-Martinez/oyster-host-microbiome-transcriptomics: Count matrices and scripts for oyster host and microbiome transcriptomic analyses. · GitHub https://www.iaea.org/services/oa-icc/science-and-collaboration/data-access-and-management OA-ICC DIF 9.4 2026-06-18 2026-07-17