PANGAEA_995496 Seawater carbonate chemistry and data collected from laboratory experiments on the tolerance of egg and yolk-sac larval yellowfin sole (Limanda aspera) to ocean warming and acidification Slesinger, Emily; Tyler, Erin F; Mundorff, Samantha; Youn, Kathryn R; Edmondson, Leo; Hurst, Thomas P Seawater carbonate chemistry and data collected from laboratory experiments on the tolerance of egg and yolk-sac larval yellowfin sole (Limanda aspera) to ocean warming and acidification 2026-06-17 PANGAEA Dataset https://doi.pangaea.de/10.1594/PANGAEA.995496 Investigator Emily Slesinger emily.slesinger@noaa.gov Investigator Yan Yang yangyan@xmu.edu.cn Earth Science Type of study Species, unique identification Species, unique identification (Semantic URI) Species, unique identification (URI) Identification Treatment: temperature Treatment Stage Temperature, water Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Citrate synthase activity, unit per protein mass b-hydroxyacyl-CoA dehydrogenase activity, unit per protein mass Lactate dehydrogenase activity, unit per protein mass Degree days Fish, standard length Fish, standard length, standard deviation Myotome height Myotome height, standard deviation Dry mass Dry mass, standard deviation Yolk area Yolk area, standard deviation Growth rate Yolk absorption rate Daily mortality rate Batch Oxygen consumption per individual Salinity Salinity, standard deviation Oxygen Oxygen, standard deviation Temperature, water, standard deviation Carbon, inorganic, dissolved Carbon, inorganic, dissolved, standard deviation Alkalinity, total Alkalinity, total, standard deviation pH, total scale pH, standard deviation Partial pressure of carbon dioxide, standard deviation Carbonate system computation flag Carbon dioxide Carbon dioxide, standard deviation Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Fugacity of carbon dioxide in seawater, standard deviation Bicarbonate ion Bicarbonate ion, standard deviation Carbonate ion Carbonate ion, standard deviation Aragonite saturation state Aragonite saturation state, standard deviation Calcite saturation state geoscientificInformation Animalia Chordata Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Growth/Morphology Laboratory experiment Limanda aspera Mortality/Survival Nekton North Pacific Ocean acidification Other metabolic rates Pelagos Reproduction Respiration Single species Temperate Temperature Calculated using seacarb after Nisumaa et al. (2010) Calculated using seacarb after Orr et al. (2018) Complete OA-ICC Ocean Acidification International Coordination Centre unrestricted CC-BY-4.0: Creative Commons Attribution 4.0 International English PANGAEA Data Publisher for Earth & Environmental Science https://www.pangaea.de/ Data Center Contact Michael Diepenbroek info@pangaea.de

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Bremen Bremen 28359 Germany online 25413 data points text/tab-separated-values Slesinger, Emily; Tyler, Erin F; Mundorff, Samantha; Youn, Kathryn R; Edmondson, Leo; Hurst, Thomas P (2026): Tolerance of Egg and Yolk‐Sac Larval Yellowfin Sole ( Limanda aspera ) to Ocean Warming and Acidification. Fisheries Oceanography, 35(4), 599-619, https://doi.org/10.1111/fog.70040 Slesinger, Emily; Tyler, Erin F; Mundorff, Samantha; Youn, Kathryn R; Edmondson, Leo; Hurst, Thomas P (2026): Data collected from laboratory experiments on the tolerance of egg and yolk-sac larval yellowfin sole (Limanda aspera) to ocean warming and acidification from 2022-07-01 to 2024-08-31 (NCEI Accession 0313059) [dataset]. NOAA National Centers for Environmental Information, https://doi.org/10.25921/CVEC-SA43 Nisumaa, Anne-Marin; Pesant, Stephane; Bellerby, Richard G J; Delille, Bruno; Middelburg, Jack J; Orr, James C; Riebesell, Ulf; Tyrrell, Toby; Wolf-Gladrow, Dieter A; Gattuso, Jean-Pierre (2010): EPOCA/EUR-OCEANS data compilation on the biological and biogeochemical responses to ocean acidification. Earth System Science Data, 2(2), 167-175, https://doi.org/10.5194/essd-2-167-2010 Yang, Yan; Brockmann, Patrick; Galdino, Carolina; Schindler, Uwe; Gazeau, Frédéric (2024): An update of data compilation on the biological response to ocean acidification and overview of the OA-ICC data portal. Earth System Science Data, 16(8), 3771-3780, https://doi.org/10.5194/essd-16-3771-2024 Yang, Yan; Hansson, L; Gattuso, Jean-Pierre (2016): Data compilation on the biological response to ocean acidification: an update. Earth System Science Data, 8(1), 79-87, https://doi.org/10.5194/essd-8-79-2016 Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2024): seacarb: seawater carbonate chemistry with R. R package version 3.3.3. https://cran.r-project.org/web/packages/seacarb/index.html

This dataset contains laboratory experiment data that were collected to investigate the tolerance of egg and yolk-sac larval yellowfin sole (Limanda aspera) to ocean warming and acidification. Across four independent trials, yellowfin sole embryos and larvae were incubated at one of six experimental treatments spanning three temperatures (9, 12, 15°C) and two pCO2 target levels (low; high), and a range of organismal and physiological responses were measured. Embryonic daily mortality rates and metabolic rates increased with increasing temperature but were not affected by ocean acidification. At hatch and at yolk absorption, morphometric measurements (length, dry weight, myotome height, yolk area) were temperature-sensitive, but the response differed across the four trials. There was a consistent increase in length-based growth and yolk absorption rates with increasing temperature across trials. All morphometric and rate-based measurements were not affected by ocean acidification. Yellowfin sole metabolic enzyme activities were measured at yolk absorption. Lactate dehydrogenase (anaerobic metabolism) and β-hydroxyacyl CoA dehydrogenase (fatty acid metabolism) both increased with increasing temperature, indicating elevated energy demand. Citrate synthase (aerobic metabolism) declined with increasing pCO2 levels, indicating potential metabolic suppression. Overall, embryonic and larval yellowfin sole demonstrated relatively high tolerance to ocean warming and acidification. This dataset is included in the OA-ICC data compilation maintained in the framework of the IAEA Ocean Acidification International Coordination Centre (see https://oa-icc.ipsl.fr). Original data were downloaded from NOAA National Centers for Environmental Information (see Source) by the OA-ICC data curator. In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2024) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2026-06-16. ** For all details see the full metadata description at "https://doi.pangaea.de/10.1594/PANGAEA.995496"!

http://mel.xmu.edu.cn/en/ https://cran.r-project.org/web/packages/seacarb/index.html seacarb: seawater carbonate chemistry with R. R package version 3.3.3 https://doi.org/10.1111/fog.70040 Tolerance of Egg and Yolk‐Sac Larval Yellowfin Sole ( Limanda aspera ) to Ocean Warming and Acidification https://doi.org/10.25921/CVEC-SA43 Data collected from laboratory experiments on the tolerance of egg and yolk-sac larval yellowfin sole (Limanda aspera) to ocean warming and acidification from 2022-07-01 to 2024-08-31 (NCEI Accession 0313059) https://doi.org/10.5194/essd-16-3771-2024 An update of data compilation on the biological response to ocean acidification and overview of the OA-ICC data portal https://doi.org/10.5194/essd-2-167-2010 Calculated using seacarb after Nisumaa et al. (2010) https://doi.org/10.5194/essd-2-167-2010 EPOCA/EUR-OCEANS data compilation on the biological and biogeochemical responses to ocean acidification https://doi.org/10.5194/essd-8-79-2016 Data compilation on the biological response to ocean acidification: an update https://en.wikipedia.org/wiki/Fish_measurement Fish, standard length https://www.iaea.org/services/oa-icc/science-and-collaboration/data-access-and-management OA-ICC https://www.sciencedirect.com/science/article/pii/S030442031830149X?via%3Dihub Calculated using seacarb after Orr et al. (2018) DIF 9.4 2026-06-17 2026-07-16