Bienhold Christina Christina.Bienhold@awi.de 0000-0003-2269-9468 Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven AWI https://www.awi.de/ https://ror.org/032e6b942 Max-Planck-Institut für Marine Mikrobiologie MPI-MM http://www.mpi-bremen.de/ https://ror.org/02385fa51 Kohlenbach Katharina 2026 Benthic prokaryotic cell densities from Polarstern expedition PS138 to the Central Arctic Ocean during August-September 2023 dataset https://doi.org/10.1594/PANGAEA.995157 2026-06-11T07:58:21 Undisturbed sediment samples were obtained with a TV-guided multiple corer (TV-MUC). Upon retrieval of the MUC, the overlying water was carefully removed from each core and the upper few centimeters of sediment were cut using a steel plate and a custom-made plastic ring into the following depth horizons: 0-1 cm, 1-5 cm, 5-10 cm. For the determination of prokaryotic cell abundances, 2 ml sediment were fixed in 9 ml formaldehyde/seawater at a final concentration of 2-4% formaldehyde and stored at 4°C. Benthic prokaryotic cell abundances were determined using the Acridine Orange Direct Count (AODC) method (Meyer-Reil, 1983, doi:10.1007/BF00395813) following the protocol described in (Boetius and Damm, 1998, doi:10.1016/S0967-0637(97)00052-6; Hoffmann et al., 2017, doi:10.3389/fmicb.2017.00266). For 0-1 cm: 3 biological replicates, and 2 technical replicate filters per sample were counted. A total of 50 grids or at least 500 cells were counted per filter. For 1-5 cm: 2 biological replicates, and 2 technical replicate filters per sample were counted. A total of 50 grids or at least 250 cells were counted per filter.For 5-10 cm: 1 biological replicate, and 2 technical replicate filters per sample were counted. A total of 50 grids or at least 100 cells were counted per filter. Because cell numbers were so low in the deeper sediment layers, these numbers need to be treated with caution. Boetius Antje antje.boetius@awi.de https://www.awi.de/en/about-us/organisation/staff/single-view/antje-boetius.html 0000-0003-2117-4176 Bienhold Christina Christina.Bienhold@awi.de 0000-0003-2269-9468 2024 The Expedition PS138 of the Research Vessel POLARSTERN to the Arctic Ocean in 2023 report Berichte zur Polar- und Meeresforschung = Reports on Polar and Marine Research 788 https://doi.org/10.57738/BzPM_0788_2024 288 pp Boetius Antje antje.boetius@awi.de https://www.awi.de/en/about-us/organisation/staff/single-view/antje-boetius.html 0000-0003-2117-4176 Damm Ellen ellen.damm@awi.de https://www.awi.de/en/about-us/organisation/staff/single-view/ellen-damm.html 0000-0002-1487-1283 1998 Benthic oxygen uptake, hydrolytic potentials and microbial biomass at the Arctic continental slope Deep Sea Research Part I: Oceanographic Research Papers 45(2-3) https://doi.org/10.1016/S0967-0637(97)00052-6 239-275 Hoffmann Katy khoffman@mpi-bremen.de 0000-0002-7714-4219 Hassenrück Christiane christiane.hassenrueck@io-warnemuende.de 0000-0003-1909-1726 Salman-Carvalho Verena vcarvalh@mpi-bremen.de Holtappels Moritz moritz.holtappels@awi.de https://www.awi.de/en/about-us/organisation/staff/single-view/moritz-holtappels.html 0000-0003-3682-1903 Bienhold Christina Christina.Bienhold@awi.de 0000-0003-2269-9468 2017 Response of bacterial communities to different detritus compositions in Arctic deep-sea sediments journal article Frontiers in Microbiology 8 https://doi.org/10.3389/fmicb.2017.00266 266 Meyer-Reil L A 1983 Benthic response to sedimentation events during autumn to spring at a shallow water station in the Western Kiel Bight journal article Marine Biology 77(3) https://doi.org/10.1007/BF00395813 247-256 31.548253 130.170545 82.908155 89.945032 84.2815635 85.93606166666667 2023-08-09T18:21:23 2023-09-18T23:05:43 -4375.0 -3726.0 irregular surface PS138_15-1 84.037314 31.548253 -3992.0 2023-08-09T18:21:23 84.023533 31.849 -3991.0 2023-08-09T21:44:56 PS138 PS138 ArcWatch-1 ArcWatch-1 Tromsø, Norway 2023-08-02T00:00:00 2023-08-02 Bremerhaven, Germany 2023-10-01T00:00:00 2023-10-01 Polarstern https://doi.org/10.17815/jlsrf-3-163 DBLK 8013132 Boetius, Antje Boetius, Antje doi:10.57738/BzPM_0788_2024 20230015 hdl:10013/epic.78df01ab-0199-44db-a12f-6255f4f00a5b https://www.awi.de/fileadmin/user_upload/MET/PolarsternExpeditionMaps/PS138.png doi:10.1594/PANGAEA.963843 Polarstern https://doi.org/10.17815/jlsrf-3-163 DBLK 8013132 Multicorer with television TVMUC hdl:10013/sensor.5913e741-27df-4bf4-b77b-18787119bc8d IceStation #1 PS138_35-1 84.918369 80.248855 -3736.0 2023-08-15T23:47:20 84.909741 80.239592 -3734.0 2023-08-16T03:37:35 PS138 PS138 ArcWatch-1 ArcWatch-1 Tromsø, Norway 2023-08-02T00:00:00 2023-08-02 Bremerhaven, Germany 2023-10-01T00:00:00 2023-10-01 Polarstern https://doi.org/10.17815/jlsrf-3-163 DBLK 8013132 Boetius, Antje Boetius, Antje doi:10.57738/BzPM_0788_2024 20230015 hdl:10013/epic.78df01ab-0199-44db-a12f-6255f4f00a5b https://www.awi.de/fileadmin/user_upload/MET/PolarsternExpeditionMaps/PS138.png doi:10.1594/PANGAEA.963843 Polarstern https://doi.org/10.17815/jlsrf-3-163 DBLK 8013132 Multicorer with television TVMUC hdl:10013/sensor.5913e741-27df-4bf4-b77b-18787119bc8d IceStation #2 PS138_59-1 84.690446 107.306687 -3726.0 2023-08-20T15:30:05 84.688536 107.307584 -3731.0 2023-08-20T18:30:42 PS138 PS138 ArcWatch-1 ArcWatch-1 Tromsø, Norway 2023-08-02T00:00:00 2023-08-02 Bremerhaven, Germany 2023-10-01T00:00:00 2023-10-01 Polarstern https://doi.org/10.17815/jlsrf-3-163 DBLK 8013132 Boetius, Antje Boetius, Antje doi:10.57738/BzPM_0788_2024 20230015 hdl:10013/epic.78df01ab-0199-44db-a12f-6255f4f00a5b https://www.awi.de/fileadmin/user_upload/MET/PolarsternExpeditionMaps/PS138.png doi:10.1594/PANGAEA.963843 Polarstern https://doi.org/10.17815/jlsrf-3-163 DBLK 8013132 Multicorer with television TVMUC hdl:10013/sensor.5913e741-27df-4bf4-b77b-18787119bc8d IceStation #3 PS138_76-1 82.908155 130.026053 -4157.0 2023-08-24T08:20:48 82.914075 130.170545 -4157.0 2023-08-24T11:32:35 PS138 PS138 ArcWatch-1 ArcWatch-1 Tromsø, Norway 2023-08-02T00:00:00 2023-08-02 Bremerhaven, Germany 2023-10-01T00:00:00 2023-10-01 Polarstern https://doi.org/10.17815/jlsrf-3-163 DBLK 8013132 Boetius, Antje Boetius, Antje doi:10.57738/BzPM_0788_2024 20230015 hdl:10013/epic.78df01ab-0199-44db-a12f-6255f4f00a5b https://www.awi.de/fileadmin/user_upload/MET/PolarsternExpeditionMaps/PS138.png doi:10.1594/PANGAEA.963843 Polarstern https://doi.org/10.17815/jlsrf-3-163 DBLK 8013132 Multicorer with television TVMUC hdl:10013/sensor.5913e741-27df-4bf4-b77b-18787119bc8d IceStation #4 PS138_98-1 85.04653 130.005473 -4298.0 2023-08-27T17:40:15 85.049786 130.166157 -4298.0 2023-08-27T20:58:08 PS138 PS138 ArcWatch-1 ArcWatch-1 Tromsø, Norway 2023-08-02T00:00:00 2023-08-02 Bremerhaven, Germany 2023-10-01T00:00:00 2023-10-01 Polarstern https://doi.org/10.17815/jlsrf-3-163 DBLK 8013132 Boetius, Antje Boetius, Antje doi:10.57738/BzPM_0788_2024 20230015 hdl:10013/epic.78df01ab-0199-44db-a12f-6255f4f00a5b https://www.awi.de/fileadmin/user_upload/MET/PolarsternExpeditionMaps/PS138.png doi:10.1594/PANGAEA.963843 Polarstern https://doi.org/10.17815/jlsrf-3-163 DBLK 8013132 Multicorer with television TVMUC hdl:10013/sensor.5913e741-27df-4bf4-b77b-18787119bc8d IceStation #5 PS138_130-1 88.494016 111.904368 -4328.0 2023-09-03T08:31:04 88.495868 111.805971 -4327.0 2023-09-03T11:45:04 PS138 PS138 ArcWatch-1 ArcWatch-1 Tromsø, Norway 2023-08-02T00:00:00 2023-08-02 Bremerhaven, Germany 2023-10-01T00:00:00 2023-10-01 Polarstern https://doi.org/10.17815/jlsrf-3-163 DBLK 8013132 Boetius, Antje Boetius, Antje doi:10.57738/BzPM_0788_2024 20230015 hdl:10013/epic.78df01ab-0199-44db-a12f-6255f4f00a5b https://www.awi.de/fileadmin/user_upload/MET/PolarsternExpeditionMaps/PS138.png doi:10.1594/PANGAEA.963843 Polarstern https://doi.org/10.17815/jlsrf-3-163 DBLK 8013132 Multicorer with television TVMUC hdl:10013/sensor.5913e741-27df-4bf4-b77b-18787119bc8d IceStation #6 PS138_156-1 89.945032 47.191598 -4238.0 2023-09-08T17:00:00 89.939416 49.438945 -4239.0 2023-09-08T20:39:58 PS138 PS138 ArcWatch-1 ArcWatch-1 Tromsø, Norway 2023-08-02T00:00:00 2023-08-02 Bremerhaven, Germany 2023-10-01T00:00:00 2023-10-01 Polarstern https://doi.org/10.17815/jlsrf-3-163 DBLK 8013132 Boetius, Antje Boetius, Antje doi:10.57738/BzPM_0788_2024 20230015 hdl:10013/epic.78df01ab-0199-44db-a12f-6255f4f00a5b https://www.awi.de/fileadmin/user_upload/MET/PolarsternExpeditionMaps/PS138.png doi:10.1594/PANGAEA.963843 Polarstern https://doi.org/10.17815/jlsrf-3-163 DBLK 8013132 Multicorer with television TVMUC hdl:10013/sensor.5913e741-27df-4bf4-b77b-18787119bc8d IceStation #7 PS138_181-1 87.90777 59.046679 -4359.0 2023-09-13T19:21:28 87.896869 58.602243 -4375.0 2023-09-13T23:03:51 PS138 PS138 ArcWatch-1 ArcWatch-1 Tromsø, Norway 2023-08-02T00:00:00 2023-08-02 Bremerhaven, Germany 2023-10-01T00:00:00 2023-10-01 Polarstern https://doi.org/10.17815/jlsrf-3-163 DBLK 8013132 Boetius, Antje Boetius, Antje doi:10.57738/BzPM_0788_2024 20230015 hdl:10013/epic.78df01ab-0199-44db-a12f-6255f4f00a5b https://www.awi.de/fileadmin/user_upload/MET/PolarsternExpeditionMaps/PS138.png doi:10.1594/PANGAEA.963843 Polarstern https://doi.org/10.17815/jlsrf-3-163 DBLK 8013132 Multicorer with television TVMUC hdl:10013/sensor.5913e741-27df-4bf4-b77b-18787119bc8d IceStation #8 PS138_208-1 85.494519 60.076459 -3886.0 2023-09-18T20:18:04 85.489135 60.133681 -3882.0 2023-09-18T23:05:43 PS138 PS138 ArcWatch-1 ArcWatch-1 Tromsø, Norway 2023-08-02T00:00:00 2023-08-02 Bremerhaven, Germany 2023-10-01T00:00:00 2023-10-01 Polarstern https://doi.org/10.17815/jlsrf-3-163 DBLK 8013132 Boetius, Antje Boetius, Antje doi:10.57738/BzPM_0788_2024 20230015 hdl:10013/epic.78df01ab-0199-44db-a12f-6255f4f00a5b https://www.awi.de/fileadmin/user_upload/MET/PolarsternExpeditionMaps/PS138.png doi:10.1594/PANGAEA.963843 Polarstern https://doi.org/10.17815/jlsrf-3-163 DBLK 8013132 Multicorer with television TVMUC hdl:10013/sensor.5913e741-27df-4bf4-b77b-18787119bc8d IceStation #9 770 CC-BY-4.0 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ Event label Event Event Latitude of event Latitude Latitude Longitude of event Longitude Longitude Date/Time of event Date/Time Date/Time Elevation of event Elevation m Elevation [m] Sample ID Sample ID Bienhold Christina Christina.Bienhold@awi.de 0000-0003-2269-9468 Sample ID Replicate, biological Repl bio Bienhold Christina Christina.Bienhold@awi.de 0000-0003-2269-9468 Repl bio Replicate, technical Repl tech Bienhold Christina Christina.Bienhold@awi.de 0000-0003-2269-9468 Repl tech Depth, sediment/rock, top/minimum Depth sed top m depth http://purl.obolibrary.org/obo/PATO_0001595 sediment http://purl.obolibrary.org/obo/ENVO_00002007 rock http://purl.obolibrary.org/obo/ENVO_00001995 minimum Length L http://dbpedia.org/resource/Length Bienhold Christina Christina.Bienhold@awi.de 0000-0003-2269-9468 Depth sed top [m] Depth, sediment/rock, bottom/maximum Depth sed bot m depth http://purl.obolibrary.org/obo/PATO_0001595 sediment http://purl.obolibrary.org/obo/ENVO_00002007 rock http://purl.obolibrary.org/obo/ENVO_00001995 maximum Length L http://dbpedia.org/resource/Length Bienhold Christina Christina.Bienhold@awi.de 0000-0003-2269-9468 Depth sed bot [m] Prokaryotes, abundance as single cells Prok cell abund 10**9 #/cm**3 abundance https://www.oxforddictionaries.com/definition/american_english/abundance Acridine Orange Direct Counting (AODC) https://en.wikipedia.org/wiki/Acridine_orange Bienhold Christina Christina.Bienhold@awi.de 0000-0003-2269-9468 Prok cell abund [10**9 #/cm**3] Comment Comment Bienhold Christina Christina.Bienhold@awi.de 0000-0003-2269-9468 Comment CurationLevelC Enhanced curation https://wiki.pangaea.de/wiki/Curation_levels citable Central Arctic Ocean Deep sea Eurasian Basin sediment biogeochemistry author117656 author26820 author31616 author34022 author44791 author518 author58847 author58848 author924 basis1 campaign3394968 campaign49189 curlevel30 event3093181 event3093208 event3093238 event3093258 event3093282 event3093323 event3093350 event3093380 event3093414 geocode1599 geocode1600 geocode1601 geocode8128 inst1398 inst32 journal10805 journal16572 journal16729 journal4349 license21 method11340 method6863 param103534 param1570 param16065 param518755 param518756 param528801 param528802 pi31616 ref128610 ref140023 ref3505 ref86887 term1073130 term1073131 term1073132 term22636 term37889 term38492 term38538 term41019 term43863 term51223 term51266 term73914 term76329 topotype6