PANGAEA_995157 Benthic prokaryotic cell densities from Polarstern expedition PS138 to the Central Arctic Ocean during August-September 2023 Bienhold, Christina; Kohlenbach, Katharina Benthic prokaryotic cell densities from Polarstern expedition PS138 to the Central Arctic Ocean during August-September 2023 2026-06-11 PANGAEA Dataset https://doi.pangaea.de/10.1594/PANGAEA.995157 Investigator Christina Bienhold Christina.Bienhold@awi.de Earth Science Event label Latitude of event Longitude of event Date/Time of event Elevation of event Sample ID Replicate, biological Replicate, technical Depth, sediment/rock, top/minimum Depth, sediment/rock, bottom/maximum Prokaryotes, abundance as single cells Comment geoscientificInformation Central Arctic Ocean Deep sea Eurasian Basin Multicorer with television PS138 PS138_130-1 PS138_15-1 PS138_156-1 PS138_181-1 PS138_208-1 PS138_35-1 PS138_59-1 PS138_76-1 PS138_98-1 sediment biogeochemistry Acridine Orange Direct Counting (AODC) Polarstern 2023-08-09 2023-09-18 Complete 82.908155 89.945032 31.548253 130.170545 access rights needed CC-BY-4.0: Creative Commons Attribution 4.0 International (License comes into effect after moratorium ends) English PANGAEA Data Publisher for Earth & Environmental Science https://www.pangaea.de/ Data Center Contact Michael Diepenbroek info@pangaea.de

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Bremen Bremen 28359 Germany online 770 data points text/tab-separated-values Boetius, Antje; Bienhold, Christina (2024): The Expedition PS138 of the Research Vessel POLARSTERN to the Arctic Ocean in 2023. Berichte zur Polar- und Meeresforschung = Reports on Polar and Marine Research, 788, 288 pp, https://doi.org/10.57738/BzPM_0788_2024 Boetius, Antje; Damm, Ellen (1998): Benthic oxygen uptake, hydrolytic potentials and microbial biomass at the Arctic continental slope. Deep Sea Research Part I: Oceanographic Research Papers, 45(2-3), 239-275, https://doi.org/10.1016/S0967-0637(97)00052-6 Hoffmann, Katy; Hassenrück, Christiane; Salman-Carvalho, Verena; Holtappels, Moritz; Bienhold, Christina (2017): Response of bacterial communities to different detritus compositions in Arctic deep-sea sediments. Frontiers in Microbiology, 8, 266, https://doi.org/10.3389/fmicb.2017.00266 Meyer-Reil, L A (1983): Benthic response to sedimentation events during autumn to spring at a shallow water station in the Western Kiel Bight. Marine Biology, 77(3), 247-256, https://doi.org/10.1007/BF00395813

Undisturbed sediment samples were obtained with a TV-guided multiple corer (TV-MUC). Upon retrieval of the MUC, the overlying water was carefully removed from each core and the upper few centimeters of sediment were cut using a steel plate and a custom-made plastic ring into the following depth horizons: 0-1 cm, 1-5 cm, 5-10 cm. For the determination of prokaryotic cell abundances, 2 ml sediment were fixed in 9 ml formaldehyde/seawater at a final concentration of 2-4% formaldehyde and stored at 4°C. Benthic prokaryotic cell abundances were determined using the Acridine Orange Direct Count (AODC) method (Meyer-Reil, 1983, doi:10.1007/BF00395813) following the protocol described in (Boetius and Damm, 1998, doi:10.1016/S0967-0637(97)00052-6; Hoffmann et al., 2017, doi:10.3389/fmicb.2017.00266). For 0-1 cm: 3 biological replicates, and 2 technical replicate filters per sample were counted. A total of 50 grids or at least 500 cells were counted per filter. For 1-5 cm: 2 biological replicates, and 2 technical replicate filters per sample were counted. A total of 50 grids or at least 250 cells were counted per filter.For 5-10 cm: 1 biological replicate, and 2 technical replicate filters per sample were counted. A total of 50 grids or at least 100 cells were counted per filter. Because cell numbers were so low in the deeper sediment layers, these numbers need to be treated with caution. ** For all details see the full metadata description at "https://doi.pangaea.de/10.1594/PANGAEA.995157"!

https://doi.org/10.1007/BF00395813 Benthic response to sedimentation events during autumn to spring at a shallow water station in the Western Kiel Bight https://doi.org/10.1016/S0967-0637(97)00052-6 Benthic oxygen uptake, hydrolytic potentials and microbial biomass at the Arctic continental slope https://doi.org/10.17815/jlsrf-3-163 Polarstern https://doi.org/10.3389/fmicb.2017.00266 Response of bacterial communities to different detritus compositions in Arctic deep-sea sediments https://doi.org/10.57738/BzPM_0788_2024 The Expedition PS138 of the Research Vessel POLARSTERN to the Arctic Ocean in 2023 https://en.wikipedia.org/wiki/Acridine_orange Acridine Orange Direct Counting (AODC) DIF 9.4 2026-06-11 2026-07-29