Smykala, Mike; Engelen, Bert; Matthiessen, Birte; Hillebrand, Helmut; Lõhmus, Kertu; Pieck, Daniela; Moorthi, Stefanie: Chlorophyll a, extracellular polymeric substance concentration and 16S rRNA gene copy numbers in sediments in response to a marine heatwave [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.994659 (dataset in review)
Abstract:
This dataset originates from a laboratory mesocosm experiment investigating the effects of simulated marine heatwaves (MHWs) on benthic microbial communities from sublittoral sediments of Spiekeroog (Wadden Sea, Germany). Surface sediments were sieved (1 mm) and incubated under controlled light and temperature conditions before exposure to three temperature treatments (19 °C control, 23 °C, and 25 °C). Following a gradual warming phase, MHW conditions were maintained for six days, followed by a recovery phase at ambient temperature. Sediment samples were collected repeatedly throughout the experiment. Surface sediments (upper 2 cm) were collected using syringe cores. Pooled samples were analyzed for chlorophyll a as a proxy for microphytobenthos biomass using ethanol extraction and spectrophotometric pigment analysis. Extracellular polymeric substances (EPS) were quantified using EDTA extraction followed by phenol–sulfuric acid carbohydrate analysis. DNA was extracted from sediment subsamples using a Qiagen PowerSoil kit. Prokaryotic abundance was estimated by quantitative PCR targeting the 16S rRNA gene (primers 519F/907R), using an Escherichia coli 16S rRNA gene standard curve. The dataset includes chlorophyll a concentrations (µg g⁻¹ dry sediment), EPS carbohydrate concentrations, and prokaryotic 16S rRNA gene copy numbers for all sampling times, elevations, and treatments.
Keyword(s):
Project(s):
Funding:
German Research Foundation (DFG), grant/award no. 379417748: FOR 2716: Spatial community ecology in highly dynamic landscapes: from island biogeography to metaecosystems
Coverage:
Latitude: 53.749384 * Longitude: 7.748740
Date/Time Start: 2024-06-26T09:00:00 * Date/Time End: 2024-06-26T09:00:00
Minimum DEPTH, sediment/rock: 0.01 m * Maximum DEPTH, sediment/rock: 0.01 m
Event(s):
MHW_mesocosm * Latitude: 53.749384 * Longitude: 7.748740 * Date/Time: 2024-06-26T09:00:00 * Location: Spiekeroog, German Bight, North Sea * Method/Device: Mesocosm experiment (MESO)
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Smykala, Mike | |||
| 2 | Type of study | Study type | Smykala, Mike | |||
| 3 | Mesocosm label | Meso label | Smykala, Mike | |||
| 4 | Treatment: temperature | T:temp | °C | Smykala, Mike | ||
| 5 | Day of experiment | DOE | day | Smykala, Mike | ||
| 6 | DEPTH, sediment/rock | Depth sed | m | Smykala, Mike | Geocode | |
| 7 | Depth, top/min | Depth top | m | Smykala, Mike | ||
| 8 | Depth, bottom/max | Depth bot | m | Smykala, Mike | ||
| 9 | Extrapolymeric substances | EPS | µg/g | Smykala, Mike | Microplate reader, BioTek, Synergy MX | |
| 10 | 16S rRNA gene, copy number, per unit sediment dry mass | 16S rRNA gene/sed dm | #/g | Smykala, Mike | quantitative polymerase chain reaction (qPCR) primer: 519F/907R; LightCycler 480, Roche | |
| 11 | Chlorophyll a, per unit sediment mass | Chl a/sed | µg/g | Smykala, Mike | Microplate reader, BioTek, Synergy MX |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
500 data points