PANGAEA_994609 Seawater carbonate chemistry and microbial ecosystem responses to alkalinity enhancement in the North Atlantic Subtropical Gyre Subhas, Adam V; Marx, Lukas; Reynolds, Sarah; Flohr, Anita; Mawji, Edward W; Brown, Peter J; Cael, B B Seawater carbonate chemistry and microbial ecosystem responses to alkalinity enhancement in the North Atlantic Subtropical Gyre 2026-05-13 PANGAEA Dataset https://doi.pangaea.de/10.1594/PANGAEA.994609 Investigator Adam V Subhas asubhas@whoi.edu Investigator Yan Yang yangyan@xmu.edu.cn Earth Science Event label Type of study Treatment Time in hours Experiment Nitrate and Nitrite Nitrite Phosphate Silicate Synechococcus Prochlorococcus Picoeukaryotes Low nucleic acid bacteria High nucleic acid bacteria Bacteria Particle distribution exponent Particle distribution exponent, standard deviation Volume, filter Carbon, total particulate Carbon, inorganic, particulate Nitrogen, total particulate Calcium carbonate/Total carbon ratio δ13C, total particulate carbon Net primary production of carbon Alkalinity, total Carbon, inorganic, dissolved Calcite saturation state Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) pH, total scale Temperature, water Salinity Carbonate system computation flag Carbon dioxide Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Bicarbonate ion Carbonate ion Aragonite saturation state geoscientificInformation Alkaline_Ocean_I Alkaline_Ocean_II Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria (<20 L) Calcification/Dissolution Community composition and diversity Entire community Experiment Laboratory experiment North Atlantic Ocean alkalinity enhancement Open ocean Pelagos Primary production/Photosynthesis Temperate Calculated using seacarb after Nisumaa et al. (2010) 2020-01-25 2020-02-29 Complete 23.5666 26.5 -76.15 -41.4666 OA-ICC Ocean Acidification International Coordination Centre unrestricted CC-BY-4.0: Creative Commons Attribution 4.0 International English PANGAEA Data Publisher for Earth & Environmental Science https://www.pangaea.de/ Data Center Contact Michael Diepenbroek info@pangaea.de

Leobener Str.

Bremen Bremen 28359 Germany online 1224 data points text/tab-separated-values Subhas, Adam V; Marx, Lukas; Reynolds, Sarah; Flohr, Anita; Mawji, Edward W; Brown, Peter J; Cael, B B (2022): Microbial ecosystem responses to alkalinity enhancement in the North Atlantic Subtropical Gyre. Frontiers in Climate, 4, 784997, https://doi.org/10.3389/fclim.2022.784997 GitHub - bbcael/subhasetal · GitHub [webpage]. https://github.com/bbcael/subhasetal Nisumaa, Anne-Marin; Pesant, Stephane; Bellerby, Richard G J; Delille, Bruno; Middelburg, Jack J; Orr, James C; Riebesell, Ulf; Tyrrell, Toby; Wolf-Gladrow, Dieter A; Gattuso, Jean-Pierre (2010): EPOCA/EUR-OCEANS data compilation on the biological and biogeochemical responses to ocean acidification. Earth System Science Data, 2(2), 167-175, https://doi.org/10.5194/essd-2-167-2010 Yang, Yan; Brockmann, Patrick; Galdino, Carolina; Schindler, Uwe; Gazeau, Frédéric (2024): An update of data compilation on the biological response to ocean acidification and overview of the OA-ICC data portal. Earth System Science Data, 16(8), 3771-3780, https://doi.org/10.5194/essd-16-3771-2024 Yang, Yan; Hansson, L; Gattuso, Jean-Pierre (2016): Data compilation on the biological response to ocean acidification: an update. Earth System Science Data, 8(1), 79-87, https://doi.org/10.5194/essd-8-79-2016 Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2024): seacarb: seawater carbonate chemistry with R. R package version 3.3.3. https://cran.r-project.org/web/packages/seacarb/index.html

We tested the ocean alkalinity enhancement response of two natural seawater mixed layer microbial communities in the North Atlantic Subtropical Gyre, one at the Western gyre boundary, and one in the middle of the gyre. We conducted 4-day microcosm incubation experiments at sea, spiked with three increasing amounts of alkaline sodium salts and a 13C-bicarbonate tracer at constant pCO2. We then measured a suite of dissolved and particulate parameters to constrain the chemical and biological response to these additions. This dataset is included in the OA-ICC data compilation maintained in the framework of the IAEA Ocean Acidification International Coordination Centre (see https://oa-icc.ipsl.fr). Original data were downloaded from Github (see Source) by the OA-ICC data curator. In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2024) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2026-05-12. ** For all details see the full metadata description at "https://doi.pangaea.de/10.1594/PANGAEA.994609"!

http://mel.xmu.edu.cn/en/ https://cran.r-project.org/web/packages/seacarb/index.html seacarb: seawater carbonate chemistry with R. R package version 3.3.3 https://doi.org/10.3389/fclim.2022.784997 Microbial ecosystem responses to alkalinity enhancement in the North Atlantic Subtropical Gyre https://doi.org/10.5194/essd-16-3771-2024 An update of data compilation on the biological response to ocean acidification and overview of the OA-ICC data portal https://doi.org/10.5194/essd-2-167-2010 Calculated using seacarb after Nisumaa et al. (2010) https://doi.org/10.5194/essd-2-167-2010 EPOCA/EUR-OCEANS data compilation on the biological and biogeochemical responses to ocean acidification https://doi.org/10.5194/essd-8-79-2016 Data compilation on the biological response to ocean acidification: an update https://github.com/bbcael/subhasetal GitHub - bbcael/subhasetal · GitHub https://www.iaea.org/services/oa-icc/science-and-collaboration/data-access-and-management OA-ICC DIF 9.4 2026-05-13 2026-06-17