Flow cytometry dataset showing the abundance of microorganisms (< 20 µm) in sectioned second year sea ice (SYI) samples taken during the MOSAiC (PS122) drift expedition in the central Arctic Ocean
type: Dataset
conforms to: http://mlcommons.org/croissant/1.0
creator: Oliver Müller
creator: Allison A Fong
creator: Lasse Mørk Olsen
  type: Person
  family name: Olsen
  given name: Lasse Mørk
creator: Clara Jule Marie Hoppe
creator: Anders Torstensson
creator: Laura Heitman
  type: Person
  family name: Heitman
  given name: Laura
  affiliation: Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven
creator: Aud Larsen
creator: Elzbieta Petelenz
  type: Person
  family name: Petelenz
  given name: Elzbieta
creator: Tatiana M Tsagaraki
creator: Kyle M J Mayers
creator: Maria Langvad
  type: Person
  family name: Langvad
  given name: Maria
creator: John Paul Balmonte
creator: Emelia Chamberlain
  type: Person
  family name: Chamberlain
  given name: Emelia
creator: Gunnar Bratbak
publisher: PANGAEA
  type: Organization
  disambiguating description: Data Publisher for Earth & Environmental Science
included in data catalog: PANGAEA
  type: DataCatalog
  disambiguating description: Data Publisher for Earth & Environmental Science
date published: 2026-04-27
additional type: dataset
description: This dataset provides the abundance of microorganisms (smaller than 20 µm) enumerated using flow cytometry (FCM) during the Multidisciplinary drifting observatory for the study of Arctic Climate (MOSAiC (PS122)) sampled from second year sea ice (SYI) core sections. For sea ice derived FCM abundance data, subsamples of 15 mL were taken from pooled ice core sections that were melted in filtered sea water and correspondingly a correction factor applied, to enumerate the abundance of microorganisms per mL of melted sea ice. Additional expedition and sampling details can be found in the ECO-overview paper (Fong et al., 2024). We thank all persons involved in the expedition of the Research Vessel Polarstern during MOSAiC in 2019-2020 (AWI_PS122_00) as listed in Nixdorf et al. (2021). […]
abstract: This dataset provides the abundance of microorganisms (smaller than 20 µm) enumerated using flow cytometry (FCM) during the Multidisciplinary drifting observatory for the study of Arctic Climate (MOSAiC (PS122)) sampled from second year sea ice (SYI) core sections. For sea ice derived FCM abundance data, subsamples of 15 mL were taken from pooled ice core sections that were melted in filtered sea water and correspondingly a correction factor applied, to enumerate the abundance of microorganisms per mL of melted sea ice. Additional expedition and sampling details can be found in the ECO-overview paper (Fong et al., 2024). We thank all persons involved in the expedition of the Research Vessel Polarstern during MOSAiC in 2019-2020 (AWI_PS122_00) as listed in Nixdorf et al. (2021). Flow cytometry (FCM) is a fast, high-throughput method to enumerate the abundance of microorganism (smaller than 20 µm). FCM uses the hydrodynamic focusing of a laminar flow to separate and line up microscopic particles. When particles pass a laser beam, the generated light scattering can be used to estimate their cell size, obtain information about cell granularity and surface characteristics and determine fluorescence from inherent pigments or applied stains, such as DNA binding ones. Photosynthetic microorganisms have auto-fluorescent pigments, such as chlorophylls which in combination with the light scattering properties (cell size) or surface properties, can be used to group them into clusters of similar or identical organism types. Heterotrophic microorganisms, including archaea, bacteria and heterotrophic nanoflagellates, and virus do not have fluorescent pigments and require staining, for example using SYBR Green to stain Nucleic Acids (DNA) in order to distinguish these cells from other organic and inorganic particles in the sample. Samples for flow cytometric analysis were taken in triplicates or quadruplicates of 1.8 mL of sample water and fixed with 36 μL 25 % glutaraldehyde (0.5 …
citation: Chlorophyll a concentrations in second year sea ice during the MOSAiC expedition (PS122) in the Central Arctic Ocean 2019-2020
citation: Sea-ice salinity, temperature, density, nutrient, oxygen and hydrogen isotope composition from the coring sites during MOSAiC leg 5 in August-September 2020
citation: Overview of the MOSAiC expedition: Ecosystem
citation: Enumeration of Marine Viruses in Culture and Natural
citation: Standards and Best Practices For Reporting Flow Cytometry Observations: a technical manual. Version 1.1
citation: MOSAiC Extended Acknowledgement
citation: Interoperable vocabulary for marine microbial flow cytometry
citation: Flow cytometric enumeration of DNA-stained oceanic planktonic protists
spatial coverage: Place
  geo: GeoShape
    box: 80.7709 -0.485 88.0708 126.3299
temporal coverage: 2019-10-28T00:00:00/2020-07-20T00:00:00
funding: Multidisciplinary drifting Observatory for the Study of Arctic Climate
  type: MonetaryGrant
  funder: Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven
funding: Multidisciplinary drifting Observatory for the Study of Arctic Climate / MOSAiC
  type: MonetaryGrant
  funder: Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven
funding: Ridges - Safe HAVens for ice-associated Flora and Fauna in a Seasonally ice-covered Arctic OCean
  type: MonetaryGrant
  funder: The Research Council of Norway
size: QuantitativeValue
  value: 5594.0
  unit text: data points
variable measured: Event label
variable measured: Campaign
variable measured: Activity description
  type: PropertyValue
  description: MOSAiC week
  subject of: DefinedTermSet
    has defined term: Activity
variable measured: DATE/TIME
  type: PropertyValue
  subject of: DefinedTermSet
    has defined term: Time
variable measured: LATITUDE
variable measured: LONGITUDE
variable measured: Device type
  type: PropertyValue
  description: Device
variable measured: Core section label
  type: PropertyValue
  description: Section label
variable measured: Core section, comment
variable measured: Depth comment
variable measured: DEPTH, ice/snow
  type: PropertyValue
  unit text: m
  subject of: DefinedTermSet
    has defined term: depth
    has defined term: ice
    has defined term: snow
    has defined term: Length
variable measured: Red only fluorescing picophytoplankton
  type: PropertyValue
  unit text: #/ml
  measurement technique: Acoustic focusing cytometer, Thermo Fisher, Attune NxT [20 mW 488 nm (blue) laser]
  subject of: DefinedTermSet
    has defined term: red
    has defined term: Thermo Fisher Scientific Attune NxT flow cytometer
variable measured: Red only fluorescing nanophytoplankton
  type: PropertyValue
  unit text: #/ml
  measurement technique: Acoustic focusing cytometer, Thermo Fisher, Attune NxT [20 mW 488 nm (blue) laser]
  subject of: DefinedTermSet
    has defined term: red
    has defined term: Thermo Fisher Scientific Attune NxT flow cytometer
variable measured: Red only fluorescing nanophytoplankton, small
  type: PropertyValue
  unit text: #/ml
  measurement technique: Acoustic focusing cytometer, Thermo Fisher, Attune NxT [20 mW 488 nm (blue) laser]
  subject of: DefinedTermSet
    has defined term: red
    has defined term: small
    has defined term: Thermo Fisher Scientific Attune NxT flow cytometer
variable measured: Red only fluorescing nanophytoplankton, large
  type: PropertyValue
  unit text: #/ml
  measurement technique: Acoustic focusing cytometer, Thermo Fisher, Attune NxT [20 mW 488 nm (blue) laser]
  subject of: DefinedTermSet
    has defined term: red
    has defined term: large
    has defined term: Thermo Fisher Scientific Attune NxT flow cytometer
variable measured: Orange and red fluorescing picophytoplankton
  type: PropertyValue
  unit text: #/ml
  measurement technique: Acoustic focusing cytometer, Thermo Fisher, Attune NxT [20 mW 488 nm (blue) laser]
  subject of: DefinedTermSet
    has defined term: orange
    has defined term: Thermo Fisher Scientific Attune NxT flow cytometer
variable measured: Orange and red fluorescing nanophytoplankton
  type: PropertyValue
  unit text: #/ml
  measurement technique: Acoustic focusing cytometer, Thermo Fisher, Attune NxT [20 mW 488 nm (blue) laser]
  subject of: DefinedTermSet
    has defined term: orange
    has defined term: Thermo Fisher Scientific Attune NxT flow cytometer
variable measured: Orange fluorescing prokaryote picophytoplankton
  type: PropertyValue
  unit text: #/ml
  measurement technique: Acoustic focusing cytometer, Thermo Fisher, Attune NxT [20 mW 488 nm (blue) laser]
  subject of: DefinedTermSet
    has defined term: Thermo Fisher Scientific Attune NxT flow cytometer
variable measured: Heterotrophic nanoflagellates
  type: PropertyValue
  unit text: #/ml
  measurement technique: Flow cytometry system, Becton Dickinson, FACSCalibur
  subject of: DefinedTermSet
    has defined term: BD Biosciences FACSCalibur Flow Cytometry System
variable measured: Heterotrophic prokaryotes
  type: PropertyValue
  unit text: #/ml
  measurement technique: Flow cytometry system, Becton Dickinson, FACSCalibur
  subject of: DefinedTermSet
    has defined term: BD Biosciences FACSCalibur Flow Cytometry System
variable measured: Heterotrophic prokaryotes with relatively low nucleic acid
  type: PropertyValue
  unit text: #/ml
  measurement technique: Flow cytometry system, Becton Dickinson, FACSCalibur
  subject of: DefinedTermSet
    has defined term: BD Biosciences FACSCalibur Flow Cytometry System
variable measured: Heterotrophic prokaryotes with relatively high nucleic acid
  type: PropertyValue
  unit text: #/ml
  measurement technique: Flow cytometry system, Becton Dickinson, FACSCalibur
  subject of: DefinedTermSet
    has defined term: BD Biosciences FACSCalibur Flow Cytometry System
variable measured: Heterotrophic prokaryotes, medium
  type: PropertyValue
  unit text: #/ml
  measurement technique: Flow cytometry system, Becton Dickinson, FACSCalibur
  subject of: DefinedTermSet
    has defined term: BD Biosciences FACSCalibur Flow Cytometry System
variable measured: Heterotrophic prokaryotes, large
  type: PropertyValue
  unit text: #/ml
  measurement technique: Flow cytometry system, Becton Dickinson, FACSCalibur
  subject of: DefinedTermSet
    has defined term: large
    has defined term: BD Biosciences FACSCalibur Flow Cytometry System
variable measured: Heterotrophic prokaryotes, very large
  type: PropertyValue
  unit text: #/ml
  measurement technique: Flow cytometry system, Becton Dickinson, FACSCalibur
  subject of: DefinedTermSet
    has defined term: large
    has defined term: BD Biosciences FACSCalibur Flow Cytometry System
keywords: flow cytometry; microbial abundance; MOSAiC20192020; MOSAiC_ECO; MOSAiC expedition; sea ice core
license: https://creativecommons.org/licenses/by/4.0/
conditions of access: unrestricted
is accessible for free: true
language: en
distribution: data matrix.tab
distribution: FileObject
  type: DataDownload
  encoding format: text/html

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affiliation: Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven

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Aud Larsen
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Kyle M J Mayers
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John Paul Balmonte
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Gunnar Bratbak
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Chlorophyll a concentrations in second year sea ice during the MOSAiC expedition (PS122) in the Central Arctic Ocean 2019-2020
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date published: 2026
additional type: dataset

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Sea-ice salinity, temperature, density, nutrient, oxygen and hydrogen isotope composition from the coring sites during MOSAiC leg 5 in August-September 2020
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creator: Mats A Granskog
date published: 2024
additional type: dataset

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Overview of the MOSAiC expedition: Ecosystem
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date published: 2024
pagination: 00135
additional type: journal article

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Enumeration of Marine Viruses in Culture and Natural
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date published: 1999
pagination: 45-52
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Standards and Best Practices For Reporting Flow Cytometry Observations: a technical manual. Version 1.1
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MOSAiC Extended Acknowledgement
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Interoperable vocabulary for marine microbial flow cytometry
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date published: 2022
pagination: 975877
is part of: Frontiers in Marine Science
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Flow cytometric enumeration of DNA-stained oceanic planktonic protists
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Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven
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Elementa - Science of the Anthropocene
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