Oliver Müller
Müller
Oliver
Allison A Fong
Fong
Allison A
Lasse Mørk Olsen
Olsen
Lasse Mørk
Clara Jule Marie Hoppe
Hoppe
Clara Jule Marie
Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven
AWI
Anders Torstensson
Torstensson
Anders
Laura Heitman
Heitman
Laura
Aud Larsen
Larsen
Aud
Elzbieta Petelenz
Petelenz
Elzbieta
Tatiana M Tsagaraki
Tsagaraki
Tatiana M
Kyle M J Mayers
Mayers
Kyle M J
Maria Langvad
Langvad
Maria
John Paul Balmonte
Balmonte
John Paul
Emelia Chamberlain
Chamberlain
Emelia
Gunnar Bratbak
Bratbak
Gunnar
Flow cytometry dataset showing the abundance of microorganisms (< 20 µm) in sectioned first year sea ice (FYI) samples taken during the MOSAiC (PS122) drift expedition in the central Arctic Ocean
PANGAEA
Data Publisher for Earth & Environmental Science
dataset
This dataset provides the abundance of microorganisms (smaller than 20 µm) enumerated using flow cytometry (FCM) during the Multidisciplinary drifting observatory for the study of Arctic Climate (MOSAiC (PS122)) sampled from first year sea ice (FYI) core sections. For sea ice derived FCM abundance data, subsamples of 15 mL were taken from pooled ice core sections that were melted in filtered sea water and correspondingly a correction factor applied, to enumerate the abundance of microorganisms per mL of melted sea ice. Additional expedition and sampling details can be found in the ECO-overview paper (Fong et al., 2024). We thank all persons involved in the expedition of the Research Vessel Polarstern during MOSAiC in 2019-2020 (AWI_PS122_00) as listed in Nixdorf et al. (2021). […]
This dataset provides the abundance of microorganisms (smaller than 20 µm) enumerated using flow cytometry (FCM) during the Multidisciplinary drifting observatory for the study of Arctic Climate (MOSAiC (PS122)) sampled from first year sea ice (FYI) core sections. For sea ice derived FCM abundance data, subsamples of 15 mL were taken from pooled ice core sections that were melted in filtered sea water and correspondingly a correction factor applied, to enumerate the abundance of microorganisms per mL of melted sea ice. Additional expedition and sampling details can be found in the ECO-overview paper (Fong et al., 2024). We thank all persons involved in the expedition of the Research Vessel Polarstern during MOSAiC in 2019-2020 (AWI_PS122_00) as listed in Nixdorf et al. (2021). Flow cytometry (FCM) is a fast, high-throughput method to enumerate the abundance of microorganism (smaller than 20 µm). FCM uses the hydrodynamic focusing of a laminar flow to separate and line up microscopic particles. When particles pass a laser beam, the generated light scattering can be used to estimate their cell size, obtain information about cell granularity and surface characteristics and determine fluorescence from inherent pigments or applied stains, such as DNA binding ones. Photosynthetic microorganisms have auto-fluorescent pigments, such as chlorophylls which in combination with the light scattering properties (cell size) or surface properties, can be used to group them into clusters of similar or identical organism types. Heterotrophic microorganisms, including archaea, bacteria and heterotrophic nanoflagellates, and virus do not have fluorescent pigments and require staining, for example using SYBR Green to stain Nucleic Acids (DNA) in order to distinguish these cells from other organic and inorganic particles in the sample. Samples for flow cytometric analysis were taken in triplicates or quadruplicates of 1.8 mL of sample water and fixed with 36 μL 25 % glutaraldehyde (0.5 % final concentration) at 4 °C in the dark for approximately 2 hours, then flash frozen in liquid nitrogen and stored at -80 °C until analysis. The abundance of pico- and nano-sized phytoplankton and heterotrophic nanoflagellates (HNF) were determined using an Attune® NxT, Acoustic Focusing Cytometer (Invitrogen by Thermo Fisher Scientific) with a 20 mW 488 nm (blue) laser. Autotrophic pico-and nano-sized plankton were counted directly after thawing and the various groups discriminated based on their red fluorescence (BL3) vs. orange fluorescence (BL2), red fluorescence (BL3) vs. side scatter (SSC) and orange fluorescence (BL2) vs. side scatter (SSC). For HNF analysis, the samples were stained with SYBR Green I for 2 h in the dark and 1-2 mL were subsequently measured at a flow rate of 500 µl min-1 following the protocol of Zubkov et al. 2007. Following the Zubkov protocol, HNF are enumerated using a fixed gate and in case of sea ice samples, there is an overlap between HNA-bacteria with very high fluorescence and HNF, which is not possible to disentangle with current methodology. The abundance of virus and bacteria was determined using a FACS Calibur (Becton Dickinson) flow cytometer with a 15 mW 480 nm (blue) laser. Prior analysis of virus and bacteria, samples were first thawed, diluted x10 and x100 with 0.2 μm filtered TE buffer (Tris 10 mM, EDTA 1 mM, pH 8), stained with a green fluorescent nucleic acid dye (SYBR Green I ; Molecular Probes, Eugene, Oregon, USA) and then incubated for 10 min at 80°C in a water bath (Marie et al. 1999). Stained samples were counted at a flow rate of around 60 µL min-1 and different groups discriminated on a biparametric plot of green florescence (BL1) vs. side scatter (SSC). This allowed to distinguish virus particles of different sizes, and different bacterial groups including low nuclear acid (LNA) and high nuclear acid (HNA) bacteria. Names of size groups of photosynthetic and heterotrophic organisms are in accordance to "Standards and Best Practices For Reporting Flow Cytometry Observations: a technical manual (Version 1.1)" (Neeley et al., 2023). A short summary is listed here: RedPico = picophytoplankton (1-2 µm); RedNano = Nanophytoplankton (2-20µm), which includes subgroups RedNano_small (2-5 µm), RedNano_large (5-20 µm); OraPico = Nanophytoplankton with more orange fluorescence; OraNano = Cryptophytes; OraPicoProk = Synechococcus; HetNano = heterotrophic nanoflagellates; HetProk = bacteria (and when present archaea); HetLNA = low nucleic acid (LNA) containing bacteria; HetHNA = high nucleic acid (HNA) containing bacteria with the subgroups HetProk_medium = HNA-bacteria subgroup with less fluorescence signal, HetProk_large = HNA-bacteria subgroup with more fluorescence signal and HetProk_verylarge = HNA-bacteria subgroup with very strong fluorescence signal.
Tina Brenneis
Brenneis
Tina
Giulia Castellani
Castellani
Giulia
Jessie Creamean
Creamean
Jessie
Elise Sayana Droste
Droste
Elise Sayana
Lena Eggers
Eggers
Lena
Jessie Gardner
Gardner
Laura Heitmann
Heitmann
Musheng Lan
Lan
Musheng
Jian Ren
Ren
Jian
Pauline Snoeijs-Leijonmalm
Snoeijs-Leijonmalm
Pauline
Susanne Spahic
Spahic
Susanne
Anders Svenson
Svenson
Anja Terbrüggen
Terbrüggen
Anja
Adam Ulfsbo
Ulfsbo
Adam
Martina Vortkamp
Vortkamp
Martina
Yanpei Zhuang
Zhuang
Yanpei
Chlorophyll a concentrations in first year sea ice during the MOSAiC expedition (PS122) in the Central Arctic Ocean 2019-2020
Evgenii Salganik
Salganik
Evgenii
Laura M Whitmore
Whitmore
Laura M
Dorothea Bauch
Bauch
Dorothea
Ulrike Dietrich
Dietrich
Ulrike
Marcel Nicolaus
Nicolaus
Marcel
Nikolai Kolabutin
Kolabutin
Nikolai
Yuhong Li
Yuhong
Kai-Uwe Ludwichowski
Ludwichowski
Kai-Uwe
Andreas Marent
Marent
Andreas
Moein Mellat
Mellat
Moein
Hanno Meyer
Meyer
Hanno
Daiki Nomura
Nomura
Daiki
Katrin Schmidt
Schmidt
Katrin
Egor Shimanchuk
Shimanchuk
Egor
Linda Thielke
Thielke
Linda
Sinhué Torres-Valdés
Torres-Valdés
Sinhué
Alison L Webb
Webb
Alison L
Mikaela Weiner
Weiner
Mikaela
Mats A Granskog
Granskog
Mats A
Sea-ice salinity, temperature, density, nutrient, oxygen and hydrogen isotope composition from the coring sites during MOSAiC leg 5 in August-September 2020
Nicole Aberle
Aberle
Nicole
Carin J Ashjian
Ashjian
Carin J
Philipp Assmy
Assmy
Philipp
Youcheng Bai
Bai
Youcheng
Dorothee C E Bakker
Bakker
Dorothee C E
Kevin R Barry
Barry
Kevin R
Stefan Bertilsson
Bertilsson
Stefan
William Boulton
Boulton
William
Jeff S Bowman
Bowman
Jeff S
Deborah Bozzato
Bozzato
Deborah
Moritz Buck
Buck
Moritz
Robert G Campbell
Campbell
Robert G
Jianfang Chen
Chen
Jianfang
Melissa Chierici
Chierici
Melissa
Astrid Cornils
Cornils
Astrid
Ellen Damm
Damm
Ellen
Klaus Dethloff
Dethloff
Klaus
Oliver Ebenhöh
Ebenhöh
Sarah Lena Eggers
Sarah Lena
Anja Engel
Engel
Hauke Flores
Flores
Hauke
Agneta Fransson
Fransson
Agneta
Stephan Frickenhaus
Frickenhaus
Stephan
Cecilia E Gelfman
Gelfman
Cecilia E
Martin Graeve
Graeve
Martin
Charlotte Havermans
Havermans
Charlotte
Céline Heuzé
Heuzé
Céline
Nicole Hildebrandt
Hildebrandt
Thomas C J Hill
Hill
Thomas C J
Mario Hoppema
Hoppema
Mario
Antonia Immerz
Immerz
Antonia
Haiyan Jin
Jin
Haiyan
Boris P Koch
Koch
Boris P
Xianyu Kong
Kong
Xianyu
Alexandra Claudia Kraberg
Kraberg
Alexandra Claudia
Benjamin Allen Lange
Lange
Benjamin Allen
Benoit Lebreton
Lebreton
Benoit
Eva Leu
Leu
Eva
Brice Loose
Loose
Brice
Wieslaw Maslowski
Maslowski
Wieslaw
Camille Mavis
Mavis
Camille
Katja Metfies
Metfies
Katja
Thomas Mock
Mock
Thomas
Barbara Niehoff
Niehoff
Barbara
Eva-Maria Nöthig
Nöthig
Eva-Maria
Marc Oggier
Oggier
Marc
Ellen Oldenburg
Oldenburg
Ilka Peeken
Peeken
Ilka
Donald K Perovich
Perovich
Donald K
Ovidiu Popa
Popa
Ovidiu
Benjamin Rabe
Rabe
Benjamin
Markus Rex
Rex
Markus
Annette Rinke
Rinke
Annette
Sebastian D Rokitta
Rokitta
Sebastian D
Björn Rost
Rost
Björn
Serdar Sakinan
Sakinan
Serdar
Fokje L Schaafsma
Schaafsma
Fokje L
Hendrik Schäfer
Schäfer
Hendrik
Katyanne M Shoemaker
Shoemaker
Katyanne M
Matthew D Shupe
Shupe
Matthew D
Jacqueline Stefels
Stefels
Jacqueline
Ran Tao
Tao
Ran
Andrew Toseland
Toseland
Andrew
Maria A van Leeuwe
van Leeuwe
Maria A
Rolf Gradinger
Gradinger
Rolf
Overview of the MOSAiC expedition: Ecosystem
Elementa - Science of the Anthropocene
journal article
Dominique Marie
Marie
Dominique
Corina P D Brussaard
Brussaard
Corina P D
Runar Thyrhaug
Thyrhaug
Runar
Daniel Vaulot
Vaulot
Daniel
Enumeration of Marine Viruses in Culture and Natural
Applied and Environmental Microbiology
Aimee Neeley
Neeley
Aimee
Inia Soto
Soto
Inia
Christopher W Proctor
Proctor
Christopher W
Standards and Best Practices For Reporting Flow Cytometry Observations: a technical manual. Version 1.1
UNESCO/IOC
report
Uwe Nixdorf
Nixdorf
Uwe
Anja Sommerfeld
Sommerfeld
Ronald Kwok
Kwok
Ronald
Gunnar Spreen
Spreen
Manfred Wendisch
Wendisch
Manfred
Andreas Herber
Herber
Marius Hirsekorn
Hirsekorn
Marius
Verena Mohaupt
Mohaupt
Verena
Katharina Weiss-Tuider
Weiss-Tuider
Katharina
Bjela König
König
Bjela
Dirk Mengedoht
Mengedoht
Dirk
Julia Regnery
Regnery
Julia
Peter Gerchow
Gerchow
Peter
Daniela Ransby
Ransby
Daniela
Thomas Krumpen
Krumpen
Anne Morgenstern
Morgenstern
Anne
Christian Haas
Haas
Christian
Torsten Kanzow
Kanzow
Torsten
Frank R Rack
Rack
Frank R
Vladimir Saitzev
Saitzev
Vladimir
Vladimir Sokolov
Sokolov
Alexander Makarov
Makarov
Alexander
Stefan Schwarze
Schwarze
Thomas Wunderlich
Wunderlich
Karsten Wurr
Wurr
Karsten
Antje Boetius
Boetius
Antje
MOSAiC Extended Acknowledgement
Zenodo
other
Melilotus Thyssen
Thyssen
Melilotus
Gérald Grégori
Grégori
Gérald
Véronique Créach
Créach
Véronique
Soumaya Lahbib
Lahbib
Soumaya
Mathilde Dugenne
Dugenne
Mathilde
Hedy M Aardema
Aardema
Hedy M
Luis Felipe Artigas
Artigas
Luis Felipe
Bangqin Huang
Huang
Bangqin
Aude Barani
Barani
Aude
Laureen Beaugeard
Beaugeard
Laureen
Amel Bellaaj-Zouari
Bellaaj-Zouari
Amel
Alfred Beran
Beran
Alfred
Raffaella Casotti
Casotti
Raffaella
Yolanda Del Amo
Del Amo
Yolanda
Michel Denis
Denis
Michel
George BJ Dubelaar
Dubelaar
George BJ
Sonja Endres
Endres
Sonja
Lumi Haraguchi
Haraguchi
Lumi
Bengt Karlson
Karlson
Bengt
Christophe Lambert
Lambert
Christophe
Arnaud Louchart
Louchart
Arnaud
Gwenaëlle Moncoiffé
Moncoiffé
Gwenaëlle
David Pecqueur
Pecqueur
David
François Ribalet
Ribalet
François
Machteld Rijkeboer
Rijkeboer
Machteld
Tina Silovic
Silovic
Ricardo Silva
Silva
Ricardo
Sophie Marro
Marro
Sophie
Heidi M Sosik
Sosik
Heidi M
Marc Sourisseau
Sourisseau
Glen A Tarran
Tarran
Glen A
Nicolas Van Oostende
Van Oostende
Nicolas
Li Zhao
Zhao
Shan Zheng
Zheng
Shan
Interoperable vocabulary for marine microbial flow cytometry
Frontiers in Marine Science
M V Zubkov
Zubkov
M V
P H Burkill
Burkill
P H
J N Topping
Topping
J N
Flow cytometric enumeration of DNA-stained oceanic planktonic protists
Journal of Plankton Research
AFMOSAiC-1_00
Multidisciplinary drifting Observatory for the Study of Arctic Climate
Multidisciplinary drifting Observatory for the Study of Arctic Climate / MOSAiC
Ridges - Safe HAVens for ice-associated Flora and Fauna in a Seasonally ice-covered Arctic OCean
The Research Council of Norway
RCN
data points
Event label
Campaign
Activity description
MOSAiC week
Activity
DATE/TIME
Time
LATITUDE
LONGITUDE
Device type
Device
Core section label
Section label
Core section, comment
Depth comment
DEPTH, ice/snow
depth
ice
snow
Length
Red only fluorescing picophytoplankton
#/ml
Acoustic focusing cytometer, Thermo Fisher, Attune NxT [20 mW 488 nm (blue) laser]
red
Thermo Fisher Scientific Attune NxT flow cytometer
Red only fluorescing nanophytoplankton
Red only fluorescing nanophytoplankton, small
small
Red only fluorescing nanophytoplankton, large
large
Orange and red fluorescing picophytoplankton
orange
Orange and red fluorescing nanophytoplankton
Orange fluorescing prokaryote picophytoplankton
Heterotrophic nanoflagellates
Flow cytometry system, Becton Dickinson, FACSCalibur
BD Biosciences FACSCalibur Flow Cytometry System
Heterotrophic prokaryotes
Heterotrophic prokaryotes with relatively low nucleic acid
Heterotrophic prokaryotes with relatively high nucleic acid
Heterotrophic prokaryotes, medium
Heterotrophic prokaryotes, large
Heterotrophic prokaryotes, very large
flow cytometry; Ice core; microbial abundance; MOSAiC20192020; MOSAiC_ECO; MOSAiC expedition
unrestricted
text/tab-separated-values
text/html