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<gco:CharacterString>This dataset provides the abundance of microorganisms (smaller than 20 µm) enumerated using flow cytometry (FCM) during the Multidisciplinary drifting observatory for the study of Arctic Climate (MOSAiC (PS122)) sampled from first year sea ice (FYI) core sections. For sea ice derived FCM abundance data, subsamples of 15 mL were taken from pooled ice core sections that were melted in filtered sea water and correspondingly a correction factor applied, to enumerate the abundance of microorganisms per mL of melted sea ice. Additional expedition and sampling details can be found in the ECO-overview paper (Fong et al., 2024). We thank all persons involved in the expedition of the Research Vessel Polarstern during MOSAiC in 2019-2020 (AWI_PS122_00) as listed in Nixdorf et al. (2021). 
Flow cytometry (FCM) is a fast, high-throughput method to enumerate the abundance of microorganism (smaller than 20 µm). FCM uses the hydrodynamic focusing of a laminar flow to separate and line up microscopic particles. When particles pass a laser beam, the generated light scattering can be used to estimate their cell size, obtain information about cell granularity and surface characteristics and determine fluorescence from inherent pigments or applied stains, such as DNA binding ones. Photosynthetic microorganisms have auto-fluorescent pigments, such as chlorophylls which in combination with the light scattering properties (cell size) or surface properties, can be used to group them into clusters of similar or identical organism types. Heterotrophic microorganisms, including archaea, bacteria and heterotrophic nanoflagellates, and virus do not have fluorescent pigments and require staining, for example using SYBR Green to stain Nucleic Acids (DNA) in order to distinguish these cells from other organic and inorganic particles in the sample. 
Samples for flow cytometric analysis were taken in triplicates or quadruplicates of 1.8 mL of sample water and fixed with 36 μL 25 % glutaraldehyde (0.5 % final concentration) at 4 °C in the dark for approximately 2 hours, then flash frozen in liquid nitrogen and stored at -80 °C until analysis. The abundance of pico- and nano-sized phytoplankton and heterotrophic nanoflagellates (HNF) were determined using an Attune® NxT, Acoustic Focusing Cytometer (Invitrogen by Thermo Fisher Scientific) with a 20 mW 488 nm (blue) laser. Autotrophic pico-and nano-sized plankton were counted directly after thawing and the various groups discriminated based on their red fluorescence (BL3) vs. orange fluorescence (BL2), red fluorescence (BL3) vs. side scatter (SSC) and orange fluorescence (BL2) vs. side scatter (SSC). For HNF analysis, the samples were stained with SYBR Green I for 2 h in the dark and 1-2 mL were subsequently measured at a flow rate of 500 µl min-1 following the protocol of Zubkov et al. 2007. Following the Zubkov protocol, HNF are enumerated using a fixed gate and in case of sea ice samples, there is an overlap between HNA-bacteria with very high fluorescence and HNF, which is not possible to disentangle with current methodology. The abundance of virus and bacteria was determined using a FACS Calibur (Becton Dickinson) flow cytometer with a 15 mW 480 nm (blue) laser. Prior analysis of virus and bacteria, samples were first thawed, diluted x10 and x100 with 0.2 μm filtered TE buffer (Tris 10 mM, EDTA 1 mM, pH 8), stained with a green fluorescent nucleic acid dye (SYBR Green I ; Molecular Probes, Eugene, Oregon, USA) and then incubated for 10 min at 80°C in a water bath (Marie et al. 1999). Stained samples were counted at a flow rate of around 60 µL min-1 and different groups discriminated on a biparametric plot of green florescence (BL1) vs. side scatter (SSC). This allowed to distinguish virus particles of different sizes, and different bacterial groups including low nuclear acid (LNA) and high nuclear acid (HNA) bacteria. Names of size groups of photosynthetic and heterotrophic organisms are in accordance to "Standards and Best Practices For Reporting Flow Cytometry Observations: a technical manual (Version 1.1)" (Neeley et al., 2023). A short summary is listed here: RedPico = picophytoplankton (1-2 µm); RedNano = Nanophytoplankton (2-20µm), which includes subgroups RedNano_small (2-5 µm), RedNano_large (5-20 µm); OraPico = Nanophytoplankton with more orange fluorescence; OraNano = Cryptophytes; OraPicoProk = Synechococcus; HetNano = heterotrophic nanoflagellates; HetProk = bacteria (and when present archaea); HetLNA = low nucleic acid (LNA) containing bacteria; HetHNA = high nucleic acid (HNA) containing bacteria with the subgroups HetProk_medium = HNA-bacteria subgroup with less fluorescence signal, HetProk_large = HNA-bacteria subgroup with more fluorescence signal and HetProk_verylarge = HNA-bacteria subgroup with very strong fluorescence signal.</gco:CharacterString>
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<gco:CharacterString>EVENT LABEL: PS122/1_8-2 * LATITUDE: 85.849566 * LONGITUDE: 120.581447 * DATE/TIME: 2019-11-18T08:14:00 * ELEVATION: -4405.7 m * O2A Registry URI: https://registry.o2a-data.de/items/5065 * CAMPAIGN: PS122/1 * BASIS: Polarstern * METHOD/DEVICE: Ice corer</gco:CharacterString>
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<gco:CharacterString>EVENT LABEL: PS122/1_9-6 * LATITUDE: 86.016025 * LONGITUDE: 120.743665 * DATE/TIME: 2019-11-25T04:50:00 * ELEVATION: -4406.1 m * O2A Registry URI: https://registry.o2a-data.de/items/5065 * CAMPAIGN: PS122/1 * BASIS: Polarstern * METHOD/DEVICE: Ice corer</gco:CharacterString>
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<gco:CharacterString>EVENT LABEL: PS122/2_17-3 * LATITUDE: 86.655367 * LONGITUDE: 112.905059 * DATE/TIME: 2019-12-23T07:49:00 * O2A Registry URI: https://registry.o2a-data.de/items/5065 * LOCATION: Arctic Ocean * CAMPAIGN: PS122/2 * BASIS: Polarstern * METHOD/DEVICE: Ice corer</gco:CharacterString>
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<gco:CharacterString>temp: temp; CH4: temp on this core; salo18: salo18; halo the club music: halo, much shorter but with skeleton layer; iso: iso; eco1a: eco1a; eco1b: eco1b; eco1c: prod; bromo: hey; eco1d: algae; tex: tex; dic: hey; rep: rep; backup : backup; eco2a: eco2a; plastic: plastic; eco2b: eco; eco2c: eco2c; meio1: meio1; rho: density; gypsium: gypsium; methox: meth; salo18 syi: syi</gco:CharacterString>
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<gco:CharacterString>ch4: temp on this one; halo: no pic; salo18: salo; iso: iso; bromo: photo; eco1a: eco1a; eco1b: eco1b; eco1c: eç; eco1d: eco1d; tex: tex; rep: 2zip; eco2a: cc; eco2b: e;  dic: c; meio: m; eco2c: e; eco1a: lead</gco:CharacterString>
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<gco:CharacterString>temp: bagged as ch4;  ch4: baggecd ch4; salo18: saal.k</gco:CharacterString>
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<gco:CharacterString>T: ds fyi temp core packed as halo; halo: halo core ds fyi +temp on it; salo18: salo18 core ds fyi; rep: repository core ds fyi; chla eco1a: chla eco1a core at ds fyi; bac: backup core ds fyi; prod eco1b: prod eco1b core ds fyi; pigs eco1c: pigs eco1c core ds fyi; algae eco1d: eco1d core at ds fyi</gco:CharacterString>
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<gco:CharacterString>EVENT LABEL: PS122/3_32-63 * LATITUDE: 86.251998 * LONGITUDE: 15.032578 * DATE/TIME: 2020-03-21T08:19:00 * O2A Registry URI: https://registry.o2a-data.de/items/5065 * LOCATION: Arctic Ocean * CAMPAIGN: PS122/3 * BASIS: Polarstern * METHOD/DEVICE: Ice corer</gco:CharacterString>
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<gco:CharacterString>BROMO: FYI DS; SALO18: FYI DS; ECO1A: FYI DS; ECO1B: FYI DS; DIC: FYI DS; DMSP: FYI DS; CH4: FYI DS</gco:CharacterString>
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<gco:CharacterString>EVENT LABEL: PS122/3_34-4 * LATITUDE: 85.365388 * LONGITUDE: 13.091900 * DATE/TIME: 2020-03-30T12:06:00 * O2A Registry URI: https://registry.o2a-data.de/items/5065 * LOCATION: Arctic Ocean * CAMPAIGN: PS122/3 * BASIS: Polarstern * METHOD/DEVICE: Ice corer</gco:CharacterString>
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<gco:CharacterString>TEX: fyi; PLT: fyi; REP: fyi repository; BAC: fyi backup; DIC: fyi dic; ECO1A: fyi; PP1: fyi pool; PP2: fyipool; PP3: fyipool; DNA3: RNA core fyi</gco:CharacterString>
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<gco:CharacterString>EVENT LABEL: PS122/3_35-11 * LATITUDE: 84.528096 * LONGITUDE: 14.130862 * DATE/TIME: 2020-04-06T09:03:00 * O2A Registry URI: https://registry.o2a-data.de/items/5065 * LOCATION: Arctic Ocean * CAMPAIGN: PS122/3 * BASIS: Polarstern * METHOD/DEVICE: Ice corer</gco:CharacterString>
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<gco:CharacterString>BROMO: FYI DS; SALO18: FYI DS; HALO: FYI DS; DIC: FYI DS; ECO1A: FYI DS; Be1: FYI DS; ECO1B: FYI DS; Be2: FYI DS; ECO2A: FYI DS; ECO2B: FYI DS; ECO1C: FYI DS; PP1: FYI DS; PP2: FYI DS; PP3: FYI DS; Be3: FYI DS; ECO2C: FYI DS; MEIO1: FYI DS; Be4: FYI DS</gco:CharacterString>
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<gco:CharacterString>EVENT LABEL: PS122/3_38-24 * LATITUDE: 83.917637 * LONGITUDE: 15.562051 * DATE/TIME: 2020-04-27T10:22:00 * O2A Registry URI: https://registry.o2a-data.de/items/5065 * LOCATION: Arctic Ocean * CAMPAIGN: PS122/3 * BASIS: Polarstern * METHOD/DEVICE: Ice corer</gco:CharacterString>
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<gco:CharacterString>BROMO: FYI DS; SACKHOLE: FYI DS. Two holes, 64 and 68 cm depth; SALO18: FYI DS; DMSP: FYI DS; ECO1A: FYI DS; ECO1B: FYI DS; DIC: FYI DS; ECO1C: FYI DS; HALO: FYI DS; ECO2A: FYI DS; ECO2B: FYI DS; ECO2C: fyi:eco2c; TEX: fyi:tex; REP: fyi:rep; MEIO1: fyi meio; RHO: FYI DS; BAC: FYI DS; PP1: FYI DS; PP2: FYI DS; PP3: FYI DS; PLA: FYI DS; OPT: FYI DS</gco:CharacterString>
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<gco:CharacterString>EVENT LABEL: PS122/3_39-7 * LATITUDE: 83.886210 * LONGITUDE: 18.240578 * DATE/TIME: 2020-05-04T07:49:00 * O2A Registry URI: https://registry.o2a-data.de/items/5065 * LOCATION: Arctic Ocean * CAMPAIGN: PS122/3 * BASIS: Polarstern * METHOD/DEVICE: Ice corer</gco:CharacterString>
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<gco:CharacterString>BROMO: FYI DS; SALO18: FYI DS; DIC: FYI DS; ECO1A: FYI DS; ECO1B: FYI DS; ECO1C: FYI DS; SACKHOLE: FYI DS; HALO: FYI DS; ECO2A: FYI DS; ECO2B: FYI DS; ECO2C: FYI DS; TEX: FYI DS; MEIO1: FYI DS; REP: FYI DS; OPT: FYI DS; BAC: FYI DS; Be1: FYI DS; Be2: FYI DS; Be3: FYI DS; Be4: FYI DS; PP1: FYI DS; PP2: FYI DS; PP3: FYI DS; RNA: FYI DS</gco:CharacterString>
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<gco:CharacterString>EVENT LABEL: PS122/4_44-134 * LATITUDE: 81.974590 * LONGITUDE: 9.350731 * DATE/TIME: 2020-06-22T10:20:45 * ELEVATION: -805.7 m * O2A Registry URI: https://registry.o2a-data.de/items/5065 * LOCATION: Arctic Ocean * CAMPAIGN: PS122/4 * BASIS: Polarstern * METHOD/DEVICE: Ice corer</gco:CharacterString>
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<gco:CharacterString>EVENT LABEL: PS122/4_46-18 * LATITUDE: 81.673447 * LONGITUDE: 5.171660 * DATE/TIME: 2020-07-06T17:10:00 * ELEVATION: -754.2 m * O2A Registry URI: https://registry.o2a-data.de/items/5065 * LOCATION: Arctic Ocean * CAMPAIGN: PS122/4 * BASIS: Polarstern * METHOD/DEVICE: Ice corer</gco:CharacterString>
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<gco:CharacterString>EVENT LABEL: PS122/4_47-16 * LATITUDE: 81.413024 * LONGITUDE: 0.281517 * DATE/TIME: 2020-07-13T14:15:15 * ELEVATION: -2138.4 m * O2A Registry URI: https://registry.o2a-data.de/items/5065 * LOCATION: Arctic Ocean * CAMPAIGN: PS122/4 * BASIS: Polarstern * METHOD/DEVICE: Ice corer</gco:CharacterString>
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<gco:CharacterString>FYI ice coring at original floe site. Plot located 5 m from 06 July 2020 coring plot.</gco:CharacterString>
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