Karnatz Josefine jkarnatz@geomar.de GEOMAR - Helmholtz Centre for Ocean Research Kiel GEOMAR http://www.geomar.de https://ror.org/02h2x0161 Barthelmeß Theresa tbarthelmess@geomar.de 0000-0001-7573-4621 GEOMAR - Helmholtz Centre for Ocean Research Kiel GEOMAR http://www.geomar.de https://ror.org/02h2x0161 Sabbaghzadeh Bita bita.online2002@gmail.com 0000-0002-1232-0576 Engel Anja aengel@geomar.de 0000-0002-1042-1955 GEOMAR - Helmholtz Centre for Ocean Research Kiel GEOMAR http://www.geomar.de https://ror.org/02h2x0161 2026 Biochemical composition and phytoplankton community of the sea surface microlayer and underlying water during ELISABETH MANN BORGESE cruise EMB295 (CenBASE) in 2022 dataset bundled publication https://doi.org/10.1594/PANGAEA.993574 2026-04-17T11:01:27 This dataset comprises samples collected in the Eastern Gotland Basin (Central Baltic Sea) during the EMB295 CenBASE research cruise conducted from June 30th to July 19th 2022. Sea surface microlayer (SML) samples were collected using established methods, i.e. the glass plate and the Garrett screen. Reference samples from the underlying water (ULW) were collected at approximately 1 m depth using a manually deployed Niskin bottle. To characterize the biochemical composition of the SML and the ULW, water samples were collected twice daily for the analysis of total organic carbon (TOC), total amino acids (TAA), total combined carbohydrates (TCCHO), and surfactants (determined using phase-sensitive AC voltammetry). TOC was measured using a TOC analyser. High-performance anion-exchange chromatography coupled to pulsed amperometric detection (HPAEC-PAD) was performed for carbohydrate analysis. Amino acids were determined using a high-performance liquid chromatography (HPLC). Chlorophyll a samples were taken from the Niskin bottles at 1m depth (ULW) and analysed using a fluorometer from Turner Designs (Turner TD 10-AU005). Abundance and community composition of nano- and picophytoplankton (<=20µm) were determined by flow cytometry. Particulate samples from the sea surface were collected using a Neuston catamaran equipped with a 20 µm mesh net, sampling the upper 20 cm of the water column. Reference particulate samples from the ULW were collected using an ULW-net deployed at approximately 1 m depth. The biochemical composition of the particulate fraction was assessed through measurements of particulate organic carbon >20µm (POC>20µm), particulate amino acids >20µm (PAA>20µm), and particulate combined carbohydrates >20µm (PCCHO>20µm). POC was measured using an Euro EA elemental analyser. To determine the composition of the microphytoplankton community (>20 µm), samples were preserved with Lugol's solution and analyzed according to the Utermöhl method. Sampling stations were subsequently classified based on the abundance of microphytoplankton (>20 µm) and nano/picophytoplankton (≤20 µm). Hydrographic parameters, including salinity and temperature, were obtained from CTD measurements at a depth of 3 m to characterize the prevailing hydrological conditions. Karnatz Josefine jkarnatz@geomar.de in review Biochemial Characteristics of the Sea Surface Microlayer in the Central Baltic Sea and Potential Signatures of Cyanobacterial Blooms journal article 19.23765 20.176119 57.18723 57.450808 19.722390634529916 57.34471052547009 2022-07-06T16:29:40 2022-07-16T05:39:36 BASS Biogeochemical processes and Air-sea exchange in the Sea-Surface microlayer https://uol.de/en/bass GEOMAR - Helmholtz Centre for Ocean Research Kiel GEOMAR http://www.geomar.de https://ror.org/02h2x0161 German Research Foundation DFG http://www.dfg.de/en/ https://doi.org/10.13039/501100001659 451574234 FOR 5267 - Biogeochemical processes and Air-sea exchange in the Sea-Surface microlayer (BASS) https://gepris.dfg.de/gepris/projekt/451574234 3 CC-BY-4.0 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ Salinity | Sal | CTD | Temperature, water | Temp | phytoplankton abundance >20µm | Classification | phytoplankton abundance <20µm | Sampling | Depth zone | Chlorophyll a | Chl a | Fluorometer, Turner Designs, 10-AU | Chlorophyll a, standard deviation | Chl a std dev | Chla_rich | Picoplankton | Picopl | Flow cytometry | Nanoplankton | Nanopl | phycoerythrin_rich | flowcyto_sum | Phytoplankton, total | Phytopl tot | Aspartic acid and Asparagine | AsX | High Performance Liquid Chromatography (HPLC) | Glutamine and Glutamic acid | GlX | Serine | Ser | Glycine | Gly | Threonine | Thr | Arginine | Arg | Alanine | Ala | gamma-Aminobutyric acid | g-ABA | Tyrosine | Tyr | Valine | Val | Isoleucine | Ile | Phenylalanine | Phe | Leucine | Leu | Amino acid, total | TAA | C | Fucose | Fuc | High Performance anion-exchange chromatography | Rhamnose | Rha | Galactosamine | GalN | Arabinose | Ara | Glucosamine | GlcN | Galactose | Gal | Glucose | Glc | Mannose/Xylose | Man/Xyl | Galacturonic acid | GalA | Glucuronic acid | GlcA | Carbohydrates, total combined | TCCHO | TX-100 | Surfactants | Surfact | Voltammetry, Metrohm, 796 VA Computrace | Carbon, organic, total | TOC | TOC analyzer (Shimadzu) | Carbon, organic, total, standard deviation | TOC std dev | microphytoplankton (>20µm) classification | nanophytoplankton (<=20µm) classification | >20µm | Carbon, organic, particulate | POC | Aspartic acid | Asp | Glutamic acid | Glu | PAA>20µm_sum [nM] | Amino acids | AA | PAA>20µm_sum [nM C] | Mannose | Man | PCCHO>20µm_sum [nM] | Carbohydrates, particulate combined | PCCHO | PCCHO>20µm_sum [nM C] | Aphanizomenon_abundance [Ind/L] | Aphanizomenon sp. | Aphanocapsa_abundance [Ind/L] | Aphanocapsa spp. | Chaetoceros_abundance [Ind/L] | Chaetoceros | Cylindrotheca_abundance [Ind/L] | Cylindrotheca | Nodularia_abundance [Ind/L] | Nodularia spumigena | N. spumigena | Pseudo-nitzschia_abundance [Ind/L] | Pseudo-nitzschia | Dinophysis_abundance [Ind/L] | Dinophysis | Diplopsalis (group)_abundance [Ind/L] | Diplopsalis-type | Diplopsalis-T | microphytoplankton_sum_abundance [Ind/L] | Microphytoplankton | Microphytopl | Aphanizomenon_biomass [µg C/L] | Aphanizomenon spp., biomass | Aphanizomenon spp. biom | Aphanocapsa_biomass [µg C/L] | Aphanocapsa sp., biomass, wet mass | Aphanocapsa sp. biom wm | Chaetoceros_biomass [µg C/L] | Chaetoceros sp., biomass as carbon | Chaetoceros sp. C | Cylindrotheca_biomass [µg C/L] | Cylindrotheca sp., biomass as carbon | Cylindrotheca sp. C | Nodularia_biomass [µg C/L] | Nodularia spp., biomass | Nodularia spp. biom | Pseudo-nitzschia_biomass [µg C/L] | Pseudo-nitzschia sp., biomass as carbon | Pseudo-nitzschia sp. C | Dinophysis_biomass [µg C/L] | Dinophysis sp., biomass as carbon | Dinophysis sp. C | Diplopsalis (group)_biomass [µg C/L] | Diplopsalis spp., biomass as carbon | Diplopsalis spp. C | cyanobacteria_sum_biomass [µg C/L] | Cyanobacteria, biomass | Cyanobacteria | microphytoplankton_sum_biomass [µg C/L] | Microphytoplankton, biomass as carbon | Microphytopl C citable air-sea interface amino acids Aphanizomenon sp. Baltic Sea carbohydrates Cyanobacteria Nodularia spumigena Phytoplankton bloom sea surface microlayer Surfactants synechococcus geomar method14201 method17222 method199 method247 method6337 method6546 method7426 param100920 param122805 param127262 param128305 param132667 param144429 param150503 param155288 param15605 param15613 param182808 param182809 param182810 param182811 param182812 param182813 param182815 param182816 param183992 param184266 param184297 param18664 param18955 param190218 param190219 param26144 param27397 param2943 param4496 param4498 param4500 param4502 param4503 param4505 param4507 param4508 param4512 param4513 param4515 param4517 param4518 param500266 param503343 param503348 param514188 param520784 param52420 param524825 param52680 param527631 param532572 param536183 param54886 param56195 param58738 param58780 param716 param717 param73744 param75533 param77073 param782 param83477 pi98880 author31027 author75807 author83182 author98880 award3120 funder20472 geocode1599 geocode1600 geocode1601 inst20147 license21 project5068 ref139302 term242 term39415 term42991 term43377 term44006 term48322 term48530 term48531 term50933 term60979 term73586 term74222