PANGAEA_992922 Seawater carbonate chemistry and microbial community dynamics over large spatial and environmental gradients in a subtropical ocean basin Anderson, Sean R; Silliman, Katherine; Barbero, Leticia; Gomez, Fabian A; Stauffer, Beth A; Schnetzer, Astrid; Kelble, Christopher R; Thompson, Luke R Seawater carbonate chemistry and microbial community dynamics over large spatial and environmental gradients in a subtropical ocean basin 2026-03-11 PANGAEA Dataset https://doi.pangaea.de/10.1594/PANGAEA.992922 Investigator Sean R Anderson seanceltics34@gmail.com Investigator Yan Yang yangyan@xmu.edu.cn Earth Science Type of study Sample code/label Group Replicate Station label Subregion LATITUDE LONGITUDE DEPTH, water Water depth description Water volume, filtered Salinity Temperature, water Oxygen Phosphate Silicate Nitrate Nitrite Ammonium Carbon, inorganic, dissolved Alkalinity, total Carbonate ion Aragonite saturation state pH, total scale Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Deoxyribonucleic acid Fluorescence Ratio Comment Carbonate system computation flag Carbon dioxide Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Bicarbonate ion Calcite saturation state geoscientificInformation Coast and continental shelf Community composition and diversity Entire community Field observation North Atlantic Open ocean Pelagos Temperate Tropical Calculated using seacarb after Nisumaa et al. (2010) Complete 18.836 30.111 79.618 97.639 2.0 m (DEPTH, water) 3326.0 m (DEPTH, water) OA-ICC Ocean Acidification International Coordination Centre unrestricted CC-BY-4.0: Creative Commons Attribution 4.0 International English PANGAEA Data Publisher for Earth & Environmental Science https://www.pangaea.de/ Data Center Contact Michael Diepenbroek info@pangaea.de

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Bremen Bremen 28359 Germany online 18138 data points text/tab-separated-values Anderson, Sean R; Silliman, Katherine; Barbero, Leticia; Gomez, Fabian A; Stauffer, Beth A; Schnetzer, Astrid; Kelble, Christopher R; Thompson, Luke R (2026): Microbial community dynamics over large spatial and environmental gradients in a subtropical ocean basin. Applied and Environmental Microbiology, 92(2), e01889-25, https://doi.org/10.1128/aem.01889-25 Nisumaa, Anne-Marin; Pesant, Stephane; Bellerby, Richard G J; Delille, Bruno; Middelburg, Jack J; Orr, James C; Riebesell, Ulf; Tyrrell, Toby; Wolf-Gladrow, Dieter A; Gattuso, Jean-Pierre (2010): EPOCA/EUR-OCEANS data compilation on the biological and biogeochemical responses to ocean acidification. Earth System Science Data, 2(2), 167-175, https://doi.org/10.5194/essd-2-167-2010 Yang, Yan; Brockmann, Patrick; Galdino, Carolina; Schindler, Uwe; Gazeau, Frédéric (2024): An update of data compilation on the biological response to ocean acidification and overview of the OA-ICC data portal. Earth System Science Data, 16(8), 3771-3780, https://doi.org/10.5194/essd-16-3771-2024 Yang, Yan; Hansson, L; Gattuso, Jean-Pierre (2016): Data compilation on the biological response to ocean acidification: an update. Earth System Science Data, 8(1), 79-87, https://doi.org/10.5194/essd-8-79-2016 Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2024): seacarb: seawater carbonate chemistry with R. R package version 3.3.3. https://cran.r-project.org/web/packages/seacarb/index.html DNA sampling in the Gulf of Mexico on GOMECC-4 [webpage]. https://zenodo.org/records/13102580 Barbero, Leticia; Stefanick, Andrew; Hooper, James; Wanninkhof, Rik; Baringer, Molly O; Smith, Ian; Zhang, Jia-Zhong; Martín-Mayor, Macarena; Byrne, Robert H; Langdon, Chris; Stauffer, Beth A (2024): Carbon-related, hydrographic data collected during th NOAA Ship Ronald H. Brown 4th Gulf of Mexico Ecosystems and Carbon Cycle (GOMECC-4) cruise in Gulf of Mexico and North Atlantic Ocean from 2021-09-13 to 2021-10-21 (NCEI Accession 0294320) [dataset]. NOAA National Centers for Environmental Information, https://doi.org/10.25921/4TWF-PP50 Silliman, Katherine; Anderson, Sean R; Thompson, Luke R (2024): eDNA from Gulf of Mexico Ecosystems and Carbon Cruise 2021 (GOMECC-4) [dataset]. United States Geological Survey, https://doi.org/10.15468/SM6FPZ

Microbes are fundamental to ocean ecosystem function, yet they remain understudied across broad spatial and environmental scales in dynamic regions like the Gulf of America/Gulf of Mexico (GOM). We employed DNA metabarcoding to characterize prokaryotes (16S V4–V5) and protists (18S V9) across 51 stations, spanning 16 inshore–offshore transects and three depths. This dataset is included in the OA-ICC data compilation maintained in the framework of the IAEA Ocean Acidification International Coordination Centre (see https://oa-icc.ipsl.fr). Original data were extracted from tables in the related paper (see Related to) by the OA-ICC data curator. In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2024) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2026-03-11. ** For all details see the full metadata description at "https://doi.pangaea.de/10.1594/PANGAEA.992922"!

http://en.wikipedia.org/wiki/ISO_6709 LATITUDE http://en.wikipedia.org/wiki/ISO_6709 LONGITUDE http://mel.xmu.edu.cn/en/ https://cran.r-project.org/web/packages/seacarb/index.html seacarb: seawater carbonate chemistry with R. R package version 3.3.3 https://doi.org/10.1128/aem.01889-25 Microbial community dynamics over large spatial and environmental gradients in a subtropical ocean basin https://doi.org/10.15468/SM6FPZ eDNA from Gulf of Mexico Ecosystems and Carbon Cruise 2021 (GOMECC-4) https://doi.org/10.25921/4TWF-PP50 Carbon-related, hydrographic data collected during th NOAA Ship Ronald H. Brown 4th Gulf of Mexico Ecosystems and Carbon Cycle (GOMECC-4) cruise in Gulf of Mexico and North Atlantic Ocean from 2021-09-13 to 2021-10-21 (NCEI Accession 0294320) https://doi.org/10.5194/essd-16-3771-2024 An update of data compilation on the biological response to ocean acidification and overview of the OA-ICC data portal https://doi.org/10.5194/essd-2-167-2010 Calculated using seacarb after Nisumaa et al. (2010) https://doi.org/10.5194/essd-2-167-2010 EPOCA/EUR-OCEANS data compilation on the biological and biogeochemical responses to ocean acidification https://doi.org/10.5194/essd-8-79-2016 Data compilation on the biological response to ocean acidification: an update https://www.iaea.org/services/oa-icc/science-and-collaboration/data-access-and-management OA-ICC https://zenodo.org/records/13102580 DNA sampling in the Gulf of Mexico on GOMECC-4 DIF 9.4 2026-03-11 2026-04-14