de.pangaea.dataset990505 und dataset PANGAEA

info@pangaea.de

https://www.pangaea.de/ information pointOfContact 2026-04-30T20:48:44Z https://doi.pangaea.de/10.1594/PANGAEA.990505 2026-02-16T14:03:19 publication https://doi.org/10.1594/PANGAEA.990505 Bunting, Imogen C

imogenbunting@gmail.com

https://orcid.org/0009-0009-5407-0163 information author D'Archino, Roberta author Barr, Neill G author Bury, Sarah J author Desmond, Matthew J author Hepburn, Christopher D author Kok, Yun Yi author Krieger, Erik C author Le, Duong Minh author Cornwall, Christopher Edward

Christopher.cornwall@uwa.edu.au

https://orcid.org/0000-0002-6154-4082 information author PANGAEA

info@pangaea.de

https://www.pangaea.de/ information publisher tableDigital In this study, juvenile sporophytes were cultured using parental material from five geographically and genetically distinct populations of the giant kelp Macrocystis pyrifera from the northern and southern edges of the species' range in Aotearoa New Zealand. These sporophytes were exposed to simulated 22-day marine heatwaves at either 18°C or 22°C, or a 14°C control. The sporophytes were then kept at 14°C for a further 21 days to observe whether they showed signs of recovery. The experiment also assessed whether the long-term storage of gametophytes under red light could affect sporophyte performance; this was achieved by comparing two cultures from the same population (Wellington) that were cultured either with or without long-term storage under red light. The experiment was carried out in Wellington, New Zealand between June and July 2024. Survival was assessed visually at the end of the heatwave and recovery phases. Length was measured with a ruler, and wet weight was measured with a balance, before and after the heatwave and after the recovery phase, and relative growth rates were calculated based on both metrics. Maximum quantum yield was measured using a Walz Diving-PAM blue light fluorometer before and after the heatwave and after the recovery phase. Blade tissue samples were taken at the end of the heatwave and recovery phases; these were oven dried, ground, and analysed using a mass spectrometer to determine total carbon and nitrogen content and δ13C and δ15N stable isotope ratios. It was found that the 22°C heatwave treatment caused 64% mortality and a significant reduction in linear growth rates across all kelp cultures, as well as a non-significant but noticeable reduction in maximum quantum yield. However, all cultures were comparatively resistant to the 18°C treatment, with just 23% mortality during the heatwave. Chemical composition of sporophytes was altered in both heatwave treatments, with total carbon content, carbon:nitrogen ratio, and δ15N values increasing significantly. There was little evidence that long-term storage of kelp gametophytes under red light impacts sporophyte survival or growth. Although survival rates and chemical composition differed between some populations, there was no consistent evidence overall for significant differences in thermal tolerance between northern and southern M. pyrifera cultures. completed Bunting, Imogen C

imogenbunting@gmail.com

https://orcid.org/0009-0009-5407-0163 information principalInvestigator giant kelp Kelp Laboratory experiment Macroalgae marine heatwaves (MHWs) New Zealand thermal tolerance theme 1995-01-01 creation PANGAEA

info@pangaea.de

https://www.pangaea.de/ information pointOfContact CoRE Coastal People : Southern Skies theme 1995-01-01 creation PANGAEA

info@pangaea.de

https://www.pangaea.de/ information pointOfContact copyright copyright CC-BY-4.0: Creative Commons Attribution 4.0 International textTable und geoscientificInformation 166.78183 174.83595 -47.3045 -40.71819 2020-05-03T00:00:00 2023-04-01T00:00:00 Event label thematicClassification Type of study thematicClassification Date/time start, experiment thematicClassification Date/time end, experiment thematicClassification Sampling date/time, experiment thematicClassification Species, unique identification thematicClassification Reference in WoRMS Species, unique identification (Semantic URI) thematicClassification Reference in WoRMS Species, unique identification (URI) thematicClassification Reference in WoRMS Treatment: temperature description thematicClassification Simulated heatwave at either 18°C or 22°C, or a 14°C control, for 22 days, followed by recovery at 14°C for 21 days. Tank number thematicClassification Tank block number thematicClassification Tanks arranged into four blocks of three; each block had a separate illumination source. Header number thematicClassification Water pumped from headers into experimental tanks. Specimen identification thematicClassification Survived heatwave thematicClassification Visual description; Y indicates sporophytes that survived to the conclusion of the 22-day heatwave; N indicates sporophytes that died during the heatwave. Survived recovery thematicClassification Visual description; Y indicates sporophytes that survived to the conclusion of the experiment; N indicates sporophytes that died during the recovery phase; S indicates sporophytes that were sacrificed for chemical analysis immediately after the heatwave. Macrocystis pyrifera, blade length physicalMeasurement Ruler tape mm mm Macrocystis pyrifera, growth rate, blade length physicalMeasurement %/day %/day Macrocystis pyrifera, biomass, wet mass physicalMeasurement Fine balance, OHAUS Corporation, Scout SPX2202 g g Macrocystis pyrifera, growth rate, biomass, wet mass physicalMeasurement %/day %/day Maximum quantum yield of photosystem II thematicClassification Pulse-Amplitude-Modulated (PAM) fluorometer, WALZ GmbH, DIVING-PAM-II/B for blue light; Saturating pulse intensity was 5500 μmol photons m²/s; pulse duration was 0.8 s. Macrocystis pyrifera, biomass as carbon physicalMeasurement Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Advantage with ConFlo IV interface; coupled to elemental analyzer, Thermo Fisher Scientific, Flash 2000; Blade tissue samples dried in an Isotherm convection oven (Esco Lifesciences Group) at 75°C , then homogenised by grinding. Analyses used either a ZeroBlank autosampler (Costech International) for low-weight samples, or a MAS 200 R autosampler (ThermoFisher Scientific) for larger samples. For low weight samples and standards, the Flash 2000 EA was configured in low volume (IVA Analysentechnik). Reference materials were used to determine stable isotope values following Paul et al. (2007). Precision was determined by the repeat analysis of the working laboratory standard DL-Leucine (DL-2-Amino-4-methylpentanoic acid, C6H13NO2, Lot 127H1084, Sigma). Data from the analysis of IPE4-4 and Kaolinite lab standards were used as a further check on precision. % % Macrocystis pyrifera, biomass as nitrogen physicalMeasurement Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Advantage with ConFlo IV interface; coupled to elemental analyzer, Thermo Fisher Scientific, Flash 2000; Blade tissue samples dried in an Isotherm convection oven (Esco Lifesciences Group) at 75°C , then homogenised by grinding. Analyses used either a ZeroBlank autosampler (Costech International) for low-weight samples, or a MAS 200 R autosampler (ThermoFisher Scientific) for larger samples. For low weight samples and standards, the Flash 2000 EA was configured in low volume (IVA Analysentechnik). Reference materials were used to determine stable isotope values following Paul et al. (2007). Precision was determined by the repeat analysis of the working laboratory standard DL-Leucine (DL-2-Amino-4-methylpentanoic acid, C6H13NO2, Lot 127H1084, Sigma). Data from the analysis of IPE4-4 and Kaolinite lab standards were used as a further check on precision. % % Macrocystis pyrifera, carbon/nitrogen ratio thematicClassification Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Advantage with ConFlo IV interface; coupled to elemental analyzer, Thermo Fisher Scientific, Flash 2000; Blade tissue samples dried in an Isotherm convection oven (Esco Lifesciences Group) at 75°C , then homogenised by grinding. Analyses used either a ZeroBlank autosampler (Costech International) for low-weight samples, or a MAS 200 R autosampler (ThermoFisher Scientific) for larger samples. For low weight samples and standards, the Flash 2000 EA was configured in low volume (IVA Analysentechnik). Reference materials were used to determine stable isotope values following Paul et al. (2007). Precision was determined by the repeat analysis of the working laboratory standard DL-Leucine (DL-2-Amino-4-methylpentanoic acid, C6H13NO2, Lot 127H1084, Sigma). Data from the analysis of IPE4-4 and Kaolinite lab standards were used as a further check on precision. Macrocystis pyrifera, δ13C physicalMeasurement Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Advantage with ConFlo IV interface; coupled to elemental analyzer, Thermo Fisher Scientific, Flash 2000; Blade tissue samples dried in an Isotherm convection oven (Esco Lifesciences Group) at 75°C , then homogenised by grinding. Analyses used either a ZeroBlank autosampler (Costech International) for low-weight samples, or a MAS 200 R autosampler (ThermoFisher Scientific) for larger samples. For low weight samples and standards, the Flash 2000 EA was configured in low volume (IVA Analysentechnik). Reference materials were used to determine stable isotope values following Paul et al. (2007). Sample δ13C values were two-point normalised using isotopic data from the daily analysis of NIST 8573 USGS40 and L-Valine #2 USGS74. Precision was determined by the repeat analysis of the working laboratory standard DL-Leucine (DL-2-Amino-4-methylpentanoic acid, C6H13NO2, Lot 127H1084, Sigma). Data from the analysis of USGS65 Glycine (values of both δ15N and δ13C) and L-Valine #2 USGS74 (value of δ15N only) were used to check accuracy and precision. Data from the analysis of IPE4-4 and Kaolinite lab standards were used as a further check on precision. ‰ ‰ Macrocystis pyrifera, δ15N physicalMeasurement Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Advantage with ConFlo IV interface; coupled to elemental analyzer, Thermo Fisher Scientific, Flash 2000; Blade tissue samples dried in an Isotherm convection oven (Esco Lifesciences Group) at 75°C , then homogenised by grinding. Analyses used either a ZeroBlank autosampler (Costech International) for low-weight samples, or a MAS 200 R autosampler (ThermoFisher Scientific) for larger samples. For low weight samples and standards, the Flash 2000 EA was configured in low volume (IVA Analysentechnik). Reference materials were used to determine stable isotope values following Paul et al. (2007). Sample δ15N values were two-point normalised using isotopic data from the daily analysis of NIST 8573 USGS40 L-glutamic acid and NIST 8548 IAEA-N2 Ammonium sulphate. Precision was determined by the repeat analysis of the working laboratory standard DL-Leucine (DL-2-Amino-4-methylpentanoic acid, C6H13NO2, Lot 127H1084, Sigma). Data from the analysis of USGS65 Glycine (values of both δ15N and δ13C) and L-Valine #2 USGS74 (value of δ15N only) were used to check accuracy and precision. Data from the analysis of IPE4-4 and Kaolinite lab standards were used as a further check on precision. ‰ ‰ Macrocystis pyrifera, biomass as carbon physicalMeasurement Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Advantage with ConFlo IV interface; coupled to elemental analyzer, Thermo Fisher Scientific, Flash 2000; Larger tissue samples were divided into two subsamples that were analysed separately. % % Macrocystis pyrifera, biomass as nitrogen physicalMeasurement Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Advantage with ConFlo IV interface; coupled to elemental analyzer, Thermo Fisher Scientific, Flash 2000; Larger tissue samples were divided into two subsamples that were analysed separately. % % Macrocystis pyrifera, carbon/nitrogen ratio thematicClassification Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Advantage with ConFlo IV interface; coupled to elemental analyzer, Thermo Fisher Scientific, Flash 2000; Larger tissue samples were divided into two subsamples that were analysed separately. Macrocystis pyrifera, δ13C physicalMeasurement Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Advantage with ConFlo IV interface; coupled to elemental analyzer, Thermo Fisher Scientific, Flash 2000; Larger tissue samples were divided into two subsamples that were analysed separately. ‰ ‰ Macrocystis pyrifera, δ15N physicalMeasurement Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Advantage with ConFlo IV interface; coupled to elemental analyzer, Thermo Fisher Scientific, Flash 2000; Larger tissue samples were divided into two subsamples that were analysed separately. ‰ ‰ text/tab-separated-values unknown https://doi.pangaea.de/10.1594/PANGAEA.990505?format=textfile download dataset The data set was checked for completeness, correctness, and consistency of metainformation. Validity of used methods was checked and - if applicable - precision and range of data. EVENT LABEL: Break_Sea_Sound * LATITUDE: -45.577160 * LONGITUDE: 166.781830 * DATE/TIME: 2020-12-18T00:00:00 * ELEVATION: -3.0 m * LOCATION: Fiordland, New Zealand * METHOD/DEVICE: Sampling by hand Kelp sori collected by hand while diving, stored in sealed plastic bags. Gametophytes stored under red light. 2020-12-18T00:00:00 EVENT LABEL: D'Urville_Island * LATITUDE: -40.718190 * LONGITUDE: 173.966550 * DATE/TIME: 2020-06-09T00:00:00 * ELEVATION: -10.0 m * LOCATION: Marlborough, New Zealand * METHOD/DEVICE: Sampling by hand Kelp sori collected by hand while diving, stored in sealed plastic bags. Gametophytes stored under red light. 2020-06-09T00:00:00 EVENT LABEL: Otago_Karitane * LATITUDE: -45.638320 * LONGITUDE: 170.671750 * DATE/TIME: 2020-05-03T00:00:00 * ELEVATION: -4.0 m * LOCATION: Otago, New Zealand * METHOD/DEVICE: Sampling by hand Kelp sori collected by hand while diving, stored in sealed plastic bags. Gametophytes stored under red light. 2020-05-03T00:00:00 EVENT LABEL: Port_Pegasus * LATITUDE: -47.304500 * LONGITUDE: 167.645830 * DATE/TIME: 2020-09-22T00:00:00 * ELEVATION: -4.0 m * LOCATION: Stewart Island, New Zealand * METHOD/DEVICE: Sampling by hand Kelp sori collected by hand while diving, stored in sealed plastic bags. Gametophytes stored under red light. 2020-09-22T00:00:00 EVENT LABEL: Scorching_Bay_Red * LATITUDE: -41.297420 * LONGITUDE: 174.835950 * DATE/TIME: 2020-10-10T00:00:00 * ELEVATION: -4.0 m * LOCATION: Wellington, New Zealand * METHOD/DEVICE: Sampling by hand Kelp sori collected by hand while diving, stored in sealed plastic bags. Gametophytes stored under red light. 2020-10-10T00:00:00 EVENT LABEL: Scorching_Bay_White * LATITUDE: -41.297420 * LONGITUDE: 174.835950 * DATE/TIME: 2023-04-01T00:00:00 * ELEVATION: -3.0 m * LOCATION: Wellington, New Zealand * METHOD/DEVICE: Sampling by hand Kelp sori collected by hand while diving, stored in sealed plastic bags. Gametophytes stored under white light only. 2023-04-01T00:00:00 Related to 2026-01-01 publication https://doi.org/10.1186/s44419-025-00002-z Bunting, Imogen C

imogenbunting@gmail.com

https://orcid.org/0009-0009-5407-0163 information author D'Archino, Roberta author Barr, Neill G author Bury, Sarah J author Desmond, Matthew J author Hepburn, Christopher D author Kok, Yun Yi author Krieger, Erik C author Le, Duong Minh author Cornwall, Christopher Edward

Christopher.cornwall@uwa.edu.au

https://orcid.org/0000-0002-6154-4082 information author documentDigital Ocean Ecosystems 2(1) 1 References 2011-01-01 publication Enríquez, Susana

susana.enriquezdominguez@gmail.com

https://orcid.org/0000-0001-5776-902X information author Borowitzka, Michael A author documentHardcopy In Suggett, D.J., Prášil, O. and Borowitzka, M.A. (Eds) Chlorophyll a fluorescence in aquatic sciences: Methods and applications. Springer. Accessed 1 May 2024. References 1976-01-01 publication https://doi.org/10.1017/S0025315400018907 Kain, Joanna M author Jones, N S author documentDigital Journal of the Marine Biological Association of the United Kingdom 56(2) 267-290 References 2019-01-01 publication https://doi.org/10.3354/meps12900 Mabin, Christopher J T https://orcid.org/0000-0002-2311-5893 information author Johnson, Craig R https://orcid.org/0000-0002-9511-905X information author Wright, Jeffrey T

jeffrey.wright@utas.edu.au

author documentDigital Marine Ecology Progress Series 614 1-19 References 2007-01-01 publication https://doi.org/10.1002/rcm.3185 Paul, Debajyoti author Skrzypek, Grzegorz https://orcid.org/0000-0002-5686-2393 information author Fórizs, István author documentDigital Rapid Communications in Mass Spectrometry 21(18) 3006-3014