<?xml version="1.0" encoding="UTF-8"?><resource xsi:schemaLocation="http://datacite.org/schema/kernel-4 http://schema.datacite.org/meta/kernel-4.3/metadata.xsd" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns="http://datacite.org/schema/kernel-4"><identifier identifierType="DOI">10.1594/PANGAEA.990365</identifier><creators><creator><creatorName>Grandjean, Juliette</creatorName><givenName>Juliette</givenName><familyName>Grandjean</familyName><affiliation affiliationIdentifierScheme="ROR" affiliationIdentifier="https://ror.org/0496vr396">Flanders Marine Institute</affiliation><affiliation affiliationIdentifierScheme="ROR" affiliationIdentifier="https://ror.org/00cv9y106">Ghent University</affiliation></creator><creator><creatorName>González‑Aravena, Marcelo</creatorName><givenName>Marcelo</givenName><familyName>González‑Aravena</familyName><affiliation affiliationIdentifierScheme="ROR" affiliationIdentifier="https://ror.org/022gs0c53">Instituto Antártico Chileno, Punta Arenas, Chile</affiliation></creator><creator><creatorName>Gallardo, Pablo</creatorName><givenName>Pablo</givenName><familyName>Gallardo</familyName><affiliation affiliationIdentifierScheme="ROR" affiliationIdentifier="https://ror.org/049784n50">Universidad de Magallanes</affiliation></creator><creator><creatorName>Dubois, Philippe</creatorName><givenName>Philippe</givenName><familyName>Dubois</familyName><affiliation affiliationIdentifierScheme="ROR" affiliationIdentifier="https://ror.org/01r9htc13">Université Libre de Bruxelles</affiliation></creator><creator><creatorName>Catarino, Ana Isabel</creatorName><givenName>Ana Isabel</givenName><familyName>Catarino</familyName><nameIdentifier schemeURI="http://orcid.org/" nameIdentifierScheme="ORCID">0000-0002-8796-0869</nameIdentifier><affiliation affiliationIdentifierScheme="ROR" affiliationIdentifier="https://ror.org/0496vr396">Flanders Marine Institute</affiliation><affiliation affiliationIdentifierScheme="ROR" affiliationIdentifier="https://ror.org/01r9htc13">Université Libre de Bruxelles</affiliation></creator></creators><titles><title>Seawater carbonate chemistry and spine regeneration, coelomic fluid parameters and RNA/DNA ratio of sea‑urchin arbacia dufresnii</title></titles><publisher>PANGAEA</publisher><publicationYear>2026</publicationYear><subjects><subject>Acid-base regulation</subject><subject>Animalia</subject><subject>Arbacia dufresnii</subject><subject>Benthic animals</subject><subject>Benthos</subject><subject>Coast and continental shelf</subject><subject>Containers and aquaria (20-1000 L or &lt; 1 m**2)</subject><subject>Echinodermata</subject><subject>Gene expression (incl. proteomics)</subject><subject>Growth/Morphology</subject><subject>Laboratory experiment</subject><subject>Other studied parameter or process</subject><subject>Single species</subject><subject>South Atlantic</subject><subject>Temperate</subject><subject subjectScheme="Parameter">Type of study</subject><subject subjectScheme="Parameter">Species, unique identification</subject><subject subjectScheme="Parameter">Species, unique identification (Semantic URI)</subject><subject subjectScheme="Parameter">Species, unique identification (URI)</subject><subject subjectScheme="Parameter">Identification</subject><subject subjectScheme="Parameter">Code</subject><subject subjectScheme="Parameter">Treatment: pH</subject><subject subjectScheme="Parameter">pH, total scale</subject><subject subjectScheme="Parameter">Date/time analysis, experiment</subject><subject subjectScheme="Parameter">Carbonate ion</subject><subject subjectScheme="Parameter">Calcite saturation state</subject><subject subjectScheme="Parameter">Aragonite saturation state</subject><subject subjectScheme="Parameter">Calcium</subject><subject subjectScheme="Parameter">Magnesium</subject><subject subjectScheme="Parameter">Ion activity, calcium, seawater</subject><subject subjectScheme="Parameter">Ion activity, magnesium, seawater</subject><subject subjectScheme="Parameter">Ion activity, carbonate, seawater</subject><subject subjectScheme="Parameter">Magnesium carbonate</subject><subject subjectScheme="Parameter">Logarithm</subject><subject subjectScheme="Parameter">Equilibrium constant, magnesium calcite dissolution</subject><subject subjectScheme="Parameter">Ion activity product</subject><subject subjectScheme="Parameter">High magnesium calcite saturation state</subject><subject subjectScheme="Parameter">Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)</subject><subject subjectScheme="Parameter">Coelomic fluid, pH</subject><subject subjectScheme="Parameter">Calcium ions, coelomic fluid</subject><subject subjectScheme="Parameter">Magnesium, coelomic fluid</subject><subject subjectScheme="Parameter">Coelomic fluid, magnesium/calcium ratio</subject><subject subjectScheme="Parameter">Gender</subject><subject subjectScheme="Parameter">RNA/DNA ratio</subject><subject subjectScheme="Parameter">Diameter</subject><subject subjectScheme="Parameter">Mass</subject><subject subjectScheme="Parameter">Height</subject><subject subjectScheme="Parameter">Calcium carbonate, mass</subject><subject subjectScheme="Parameter">Number</subject><subject subjectScheme="Parameter">Salinity</subject><subject subjectScheme="Parameter">Temperature, water</subject><subject subjectScheme="Parameter">Alkalinity, total</subject><subject subjectScheme="Parameter">Carbonate system computation flag</subject><subject subjectScheme="Parameter">Carbon dioxide</subject><subject subjectScheme="Parameter">Fugacity of carbon dioxide (water) at sea surface temperature (wet air)</subject><subject subjectScheme="Parameter">Bicarbonate ion</subject><subject subjectScheme="Parameter">Carbon, inorganic, dissolved</subject><subject subjectScheme="Method">Experiment</subject><subject subjectScheme="Method">Calculated using seacarb after Nisumaa et al. (2010)</subject><subject subjectScheme="Project">Ocean Acidification International Coordination Centre (OA-ICC)</subject></subjects><contributors><contributor contributorType="DataCollector"><contributorName>Yang, Yan</contributorName><givenName>Yan</givenName><familyName>Yang</familyName><nameIdentifier schemeURI="http://orcid.org/" nameIdentifierScheme="ORCID">0000-0003-4900-5606</nameIdentifier></contributor></contributors><dates><date dateType="Collected">2008-11-03T00:00:00/2009-09-09T00:00:00</date></dates><resourceType resourceTypeGeneral="Dataset">Dataset</resourceType><relatedIdentifiers><relatedIdentifier relatedIdentifierType="DOI" relationType="References">10.1007/s00300-025-03437-y</relatedIdentifier><relatedIdentifier relatedIdentifierType="DOI" relationType="IsDerivedFrom">10.14284/714</relatedIdentifier><relatedIdentifier relatedIdentifierType="DOI" relationType="References">10.5194/essd-2-167-2010</relatedIdentifier><relatedIdentifier relatedIdentifierType="DOI" relationType="References">10.5194/essd-16-3771-2024</relatedIdentifier><relatedIdentifier relatedIdentifierType="DOI" relationType="References">10.5194/essd-8-79-2016</relatedIdentifier><relatedIdentifier relatedIdentifierType="URL" relationType="References">https://cran.r-project.org/web/packages/seacarb/index.html</relatedIdentifier></relatedIdentifiers><sizes><size>10649 data points</size></sizes><formats><format>text/tab-separated-values</format></formats><rightsList><rights rightsURI="https://creativecommons.org/licenses/by/4.0/" schemeURI="https://spdx.org/licenses/" rightsIdentifierScheme="SPDX" rightsIdentifier="CC-BY-4.0">Creative Commons Attribution 4.0 International</rights></rightsList><descriptions><description descriptionType="Abstract">The goal of this work was to assess the effects of Ocean Acidification (OA) on Arbacia dufresnii, a sub-Antarctic sea urchin species. Adult specimens were exposed to three pH treatments: 7.4, 7.7, and 8.0 (control), for 21 up to 23 days. We assessed spine regeneration, a proxy of calcification, by cutting spines at the base of the shaft and evaluating the magnesium content, height, and weight of the regenerated part. The coelomic fluid was sampled for pH assessment and magnesium and calcium content analysis. The RNA/DNA ratio, a proxy of metabolic activity, was assessed in the gonads and body walls.<br/>This dataset is included in the OA-ICC data compilation maintained in the framework of the IAEA Ocean Acidification International Coordination Centre (see https://oa-icc.ipsl.fr). Original data were downloaded from Marine Data Archive, VLIZ, Belgium (see Source) by the OA-ICC data curator. In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2024) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2026-01-30.</description></descriptions><geoLocations><geoLocation><geoLocationPoint><pointLongitude>-70.9333</pointLongitude><pointLatitude>-53.6166</pointLatitude></geoLocationPoint></geoLocation></geoLocations></resource>